Acta Protozoologica最新文献_第3页

Molecular Characterization of Two Myxosporean Species, Henneguya namae Haldar et al. 1983 and Myxobolus sophorae Jayasri, 1982 (Myxosporea: Myxobolidae) 两种粘孢子虫的分子特性，Henneguya namae Haldar et al. 1983和Myxobolus sophorae Jayasri, 1982(粘孢子目:粘孢子科)

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2020-01-01 DOI: 10.4467/16890027ap.20.003.12159

Anupma Garg, A. Chaudhary, Abhishek Gupta, Abhinav Kumar, B. Sharma, H. S. Singh

{"title":"Molecular Characterization of Two Myxosporean Species, Henneguya namae Haldar et al. 1983 and Myxobolus sophorae Jayasri, 1982 (Myxosporea: Myxobolidae)","authors":"Anupma Garg, A. Chaudhary, Abhishek Gupta, Abhinav Kumar, B. Sharma, H. S. Singh","doi":"10.4467/16890027ap.20.003.12159","DOIUrl":"https://doi.org/10.4467/16890027ap.20.003.12159","url":null,"abstract":"In Indian freshwater fish myxosporean infections are among the most cosmopolitan parasites, they are relatively well studied morphologically but their phylogenetic relationships were unclear and the genetic data is limited only to a few species. The study aims to present molecular data for two myxosporean species, Henneguya namae Haldar et al. 1983 and Myxobolus sophorae Jayasri, 1982 collected from Indian freshwater fish, the elongate glass-perchlet Chanda nama (=Ambassis nama) and pool barb Puntius sophore, respectively. In the present study molecular data are provided for H. namae and M. sophorae using nested PCR. The obtained partial 18S rDNA gene sequences were analyzed using maximum likelihood (ML) and Bayesian inference (BI) methods. The 18S rDNA gene sequences of H. namae showed similarity with the sequences of H. chaudhuryi, Henneguya sp. RA-2015, H. voronini and H. setiuensis about 72.1 to 78% and M. sophorae with Myxobolus ticto was about 90% respectively. The aim of this paper was to identify H. namae and M. sophorae morphologically and using molecular methods.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"59 1","pages":"39-53"},"PeriodicalIF":0.5,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70978203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Spirostomum teres: A Long Term Study of an Anoxic-Hypolimnion Population Feeding upon Photosynthesizing Microorganisms 圆螺旋体:一个以光合作用微生物为食的缺氧-低阴离子种群的长期研究

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2020-01-01 DOI: 10.4467/16890027ap.20.002.12158

Miroslav Macek, Ximena Sánchez Medina, A. Picazo, Dana Pestová, F. Reyes, Jorge Hernández, J. Alcocer, M. M. Ibarra, A. Camacho

引用次数: 6

A New Testate Amoeba, Matsakision ogawaraensis sp. nov. (Silicofilosea: Incertae sedis Euglyphida) from Lake Shore Sand of Northern Japan 日本北部湖岸沙发现的一种新遗存变形虫，Matsakision ogawaraensis sp. 11(硅藻海:Incertae sedis Euglyphida)

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2020-01-01 DOI: 10.4467/16890027AP.20.008.12676

Y. Aoki, Yoichiro Sogame, M. Wanner, Y. Mazei, S. Shimano

引用次数: 1

Antifreeze Water-Rich Dormant Cysts of the Terrestrial Ciliate Colpoda cucullus Nag-1 at −65 ℃: Possible Involvement of Ultra-Antifreeze Polysaccharides - 65℃条件下陆生纤毛虫Colpoda cucullus Nag-1富防冻水休眠囊:超防冻多糖可能参与

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2020-01-01 DOI: 10.4467/16890027ap.20.011.13266

T. Matsuoka, Yoichiro Sogame, Rikiya Nakamura, Yuya Hasegawa, M. Arikawa, F. Suizu

{"title":"Antifreeze Water-Rich Dormant Cysts of the Terrestrial Ciliate Colpoda cucullus Nag-1 at −65 ℃: Possible Involvement of Ultra-Antifreeze Polysaccharides","authors":"T. Matsuoka, Yoichiro Sogame, Rikiya Nakamura, Yuya Hasegawa, M. Arikawa, F. Suizu","doi":"10.4467/16890027ap.20.011.13266","DOIUrl":"https://doi.org/10.4467/16890027ap.20.011.13266","url":null,"abstract":"We found that the water-rich (osmolality below 0.052 Osm/l) wet resting cysts of the soil ciliate Colpoda cucullus Nag-1 were tolerant to extremely low temperature (−65℃). When cell fluid obtained from the resting cysts was cooled at −65℃, small particles of ice crystals did not grow into large ice crystals. At −65℃, the cysts shrank due to an outflow of water, because a vapor pressure difference was produced between the cell interior and freezing surrounding medium. The osmolality of these shrunk cells was estimated 0.55 Osm/l, and the freezing point depression of the shrunk cell fluid was estimated to be 1.02℃. Hence, the antifreeze ability of wet cysts at −65℃can not be explained by freezing point depression due to elevation of cytoplasmic osmolality. The cytoplasm of resting cysts was vividly stained red with periodic acid-Schiff (PAS) and stained purple with toluidine blue. On the other hand, the excystment-induced cysts were not stained with PAS, and exhibited a loss of the antifreeze activity. PAS staining of SDSPAGE gel obtained from encysting Colpoda cells showed that a large amount of PAS-positive macromolecules accumulated as the encystment stage progressed. These results suggest that antifreeze polysaccharides may be involved in the antifreeze activity of C. cucullus Nag-1 dormant forms.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70978473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Immunocytochemical Analysis of α-Tubulin Distribution Before and After Rapid Axopodial Contraction in the Centrohelid Raphidocystis contractilis Raphidocystis快速轴足收缩前后α-微管蛋白分布的免疫细胞化学分析

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2020-01-01 DOI: 10.4467/16890027ap.20.001.12157

Risa Ikeda, M. Kurokawa, Momoka Murai, Noboru Saito, M. Ando

{"title":"Immunocytochemical Analysis of α-Tubulin Distribution Before and After Rapid Axopodial Contraction in the Centrohelid Raphidocystis contractilis","authors":"Risa Ikeda, M. Kurokawa, Momoka Murai, Noboru Saito, M. Ando","doi":"10.4467/16890027ap.20.001.12157","DOIUrl":"https://doi.org/10.4467/16890027ap.20.001.12157","url":null,"abstract":"The centrohelid Raphidocystis contractilis is a heliozoan that has many radiating axopodia, each containing a bundle of microtubules. Although the rapid contraction of the axopodia at nearly a video rate (30 frames/s) is induced by mechanical stimuli, the mechanism underlying this phenomenon in R. contractilis has not yet been elucidated. In the present study, we described for the first time an adequate immunocytochemical fixation procedure for R. contractilis and the cellular distribution of α-tubulin before and after rapid axopodial contraction. We developed a flow-through chamber equipped with a micro-syringe pump that allowed the test solution to be injected at a flow rate below the threshold required to induce rapid axopodial contraction. Next, we used this injection method for evaluating the effects of different combinations of two fixatives (paraformaldehyde or glutaraldehyde) and two buffers (phosphate buffer or PHEM) on the morphological structure of the axopodia. A low concentration of glutaraldehyde in PHEM was identified as an adequate fixative for immunocytochemistry. The distribution of α-tubulin before and after rapid axopodial contraction was examined using immunocytochemistry and confocal laser scanning fluorescence microscopy. Positive signals were initially detected along the extended axopodia from the tips to the bases and were distributed in a non-uniform manner within the axopodia. Conversely, after the induction of a rapid axopodial contraction, these positive signals accumulated in the peripheral region of the cell. These results indicated that axopodial microtubules disassemble into fragments and/ or tubulin subunits during rapid axopodial contraction. Therefore, we hypothesize that the mechanism of extremely rapid axopodial contraction accompanied by cytoskeletal microtubule degradation in R. contractilis involves microtubule-severing at multiple sites.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"59 1","pages":"1-12"},"PeriodicalIF":0.5,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70978145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evidence of Stress Recovery in Free-Living Ciliate Colpoda cucullus: The Repair Capability of Resting Cysts to Damage Caused by Gamma Irradiation 自由生活的瓜小蠊应激恢复的证据：静息囊对γ射线损伤的修复能力

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2019-07-25 DOI: 10.4467/16890027ap.19.006.10837

Yoichiro Sogame, R. Saito, R. Koizumi, Taiga Shimizu, Taiki Ono

引用次数: 6

Trichodinid Ectoparasites (Ciliophora: Peritrichia) of Non-native Pumpkinseed (Lepomis gibbosus) in Europe 欧洲非本地南瓜（Lepomis gibbosus）的外毛滴虫（Ciliophora：Peritrichia）

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2019-07-25 DOI: 10.4467/16890027AP.19.009.11418

V. Yuryshynets, M. Ondračková, Y. Kvach, G. Masson

引用次数: 4

In vitro Trypanosoma cruzi Growth Inhibition by Extremely Low-frequency Electromagnetic Fields 极低频电磁场对克鲁兹锥虫体外生长的抑制作用

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2019-07-25 DOI: 10.4467/16890027AP.19.008.11417

J. Heredia-Rojas, A. O. Fuente, R. Gomez-flores, Merary Alvarez-Rodríguez, Z. J. Molina-Garza, M. Beltcheva, Omar Heredia-Rodríguez, L. Galaviz-Silva

引用次数: 0

Morpho-molecular Characterization of the Litostomatean Predatory Ciliate Phialina pupula (Müller, 1773) Foissner, 1983 (Haptoria, Lacrymariidae) Litstomatatean捕食性小纤毛虫Phialina pupula的形态分子特征（Müller，1773）Foissner，1983（Haptoria，Lacrymaridae）

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2019-07-25 DOI: 10.4467/16890027ap.19.004.10835

Ľ. Rajter, W. Bourland, P. Vďačný

{"title":"Morpho-molecular Characterization of the Litostomatean Predatory Ciliate Phialina pupula (Müller, 1773) Foissner, 1983 (Haptoria, Lacrymariidae)","authors":"Ľ. Rajter, W. Bourland, P. Vďačný","doi":"10.4467/16890027ap.19.004.10835","DOIUrl":"https://doi.org/10.4467/16890027ap.19.004.10835","url":null,"abstract":"The morphology and phylogenetic position of a haptorian ciliate, Phialina pupula (Müller, 1773) Foissner, 1983, isolated from microaerobic sandy sediments of the floodplain area of the Boise River, Idaho, U.S.A., were studied using live observation, protargol impregnation, scanning electron microscopy, and the 18S rRNA gene as well as the ITS region. The Boise population of P. pupula is characterized by a size of about 60–130 × 20–50 μm, an elliptical macronucleus with a single micronucleus, highly refractive dumbbell-shaped inclusions scattered throughout the cytoplasm and concentrated in the anterior body half, a single subterminal/terminal contractile vacuole, about 10 μm long rod-shaped extrusomes, and an average of 15 ciliary rows. In phylogenetic analyses, the newly obtained sequences from P. pupula and Lacrymaria olor clustered within the family Lacrymariidae with full to moderate statistical support. Neither the genus Phialina nor the genus Lacrymaria was depicted monophyletic both in the single gene and multigene phylogenetic inferences. Specifically, the genus Phialina was shown as a paraphyletic assemblage containing members of the polyphyletic genus Lacrymaria. This indicates that the phialinid bauplan, i.e., an anterior body end differentiated into a head-like structure directly attached to the trunk, might represent the ground pattern in the family Lacrymariidae. On the other hand, the long highly contractile neck carrying the head-like structure probably evolved later and convergently in multiple Lacrymaria species from Phialina-like ancestors.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2019-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47867358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Cluster Analysis of Non-conserved Proteins of Trypanosoma cruzi Reference Strains displays Parity Between these Groupings (Peptidemes) and the Consensually Accepted Parasite Lineages 克氏锥虫参考菌株非保守蛋白的聚类分析显示这些类群(肽)与公认的寄生虫谱系之间的相似性

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2019-07-25 DOI: 10.4467/16890027AP.19.011.11420

F. S. Coelho, Danielle P. Vieira, A. Lopes, M. Sousa

引用次数: 0