Acta Protozoologica最新文献

Diagnostic performance of loop-mediated isothermal amplification (LAMP) and Ultra-sensitive PCR in diagnosis of malaria in western Saudi Arabia 环介导等温扩增(LAMP)和超敏PCR在沙特阿拉伯西部疟疾诊断中的应用

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2023-06-23 DOI: 10.4467/16890027ap.23.002.17903

Rasha H. Solimam, A. Martín-Ramírez, J. Rubio, E. Khalifa, Bushra E. Hussein, Maha M. Wahab, M. Lanza, Yousry A. Hawash

{"title":"Diagnostic performance of loop-mediated isothermal amplification (LAMP) and Ultra-sensitive PCR in diagnosis of malaria in western Saudi Arabia","authors":"Rasha H. Solimam, A. Martín-Ramírez, J. Rubio, E. Khalifa, Bushra E. Hussein, Maha M. Wahab, M. Lanza, Yousry A. Hawash","doi":"10.4467/16890027ap.23.002.17903","DOIUrl":"https://doi.org/10.4467/16890027ap.23.002.17903","url":null,"abstract":"Malaria diagnosis continues to be one of the most important steps in the cycle of control specially in endemic countries with low parasitic load infections. Loop-mediated isothermal amplification (LAMP) and ultrasensitive PCR (Us-PCR) are two promising candidates for malaria diagnosis. A cross sectional study performed at King Faisal Hospital, Taif KSA involved patients suffering from signs and symptoms suggesting of malaria, 35 blood samples diagnosed by Nested Multiplex PCR as a reference method (13 P. falciparum, 17 P. vivax, 3 mixed P. falciparum and P. vivax) plus two negative controls were selected to be included in this study to analyse the performance of two LAMP methods (LAMP OptiGene® and LAMP WarmStart®) and two ultrasensitive PCRs (Us-PCR TARE-2 and Us-PCR Var-ATS). LAMP OptiGene® and LAMP WarmStart® performances were identical and better than the performance of Us PCR TARE 2 and Us-PCR var-ATS for P. falciparum, achieving 93.75% sensitivity, 100% specificity and 97.17% accuracy versus 87.5% sensitivity, 100% specificity and 94.29% accuracy for the Us PCR TARE 2 and 81.25% sensitivity, 94.74% specificity and 88.57% accuracy for the Us PCR var-ATS respectively. In P. vivax diagnosis LAMP OptiGene® performed excellently with 100% sensitivity, specificity, and accuracy while LAMP WarmStart® and Us-PCR Cox1 achieved 100% sensitivity, specificity 93.33% and 97.14% accuracy. The study results highlighted the benefits of using LAMP techniques for field diagnosis of malaria in different settings where the need for a more sensitive and reliable molecular tool is mandatory but at the same time removing the high cost, long turnaround time and the need of highly specialized trained technicians to perform more sophisticated molecular techniques.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43170044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On Papulifères, putative ciliate cysts of diverse morphologies, with new observations from the plankton of the Chukchi Sea (Arctic Ocean) 基于楚科奇海(北冰洋)浮游生物新观测的多种形态的假定纤毛虫囊

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2023-04-26 DOI: 10.4467/16890027ap.23.001.17690

J. Dolan, E. Yang, Jong-Kuk Moon

{"title":"On Papulifères, putative ciliate cysts of diverse morphologies, with new observations from the plankton of the Chukchi Sea (Arctic Ocean)","authors":"J. Dolan, E. Yang, Jong-Kuk Moon","doi":"10.4467/16890027ap.23.001.17690","DOIUrl":"https://doi.org/10.4467/16890027ap.23.001.17690","url":null,"abstract":"In the early 20th century, Alphonse Meunier described \"Papulifères\" as a group of enigmatic forms of unknown taxonomic affinity characterized by possessing a hyaline pimple, a \"papula\". In the early 1980's Papulifères were equated with cysts of tintinnid ciliates. The most conspicuous forms, the large Fusopsis, have been widely found, and are now known to resemble the cyst of a certain species of oligotrich ciliate (i.e., Cyrtostrombidium boreale). Thus today, Papulifère forms are often assumed to be cysts of oligotrich ciliates. Here we report on 26 Papulifère forms, of more or less distinct morphologies, found in the plankton of the Chukchi Sea. We found forms resembling some of those described by Meunier, and recorded here for the first time since Meunier's reports, and others that do not resemble any of Meunier's Papulifères. Here, we first review the literature on Papulifères, then we present the surprising variety of forms we found in Chukchi Sea, and for some, we provide for the first time data on morphological variability. With this report we have expanded the catalogue of observed Papulifère forms and documented variability in the dimensions of some morphotypes. However, we urge caution in assigning a ciliate species name to any given Papulifère form in the absence of corroborating data. There is a need for observational and/or sequence-based data to elucidate the identity of Papulifère forms.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43997036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of successful Naegleria detection from environmental resources of some selected areas of Rawlakot, Azad Jammu and Kashmir, Pakistan 在巴基斯坦阿扎德查谟和克什米尔的Rawlakot一些选定地区的环境资源中成功检测到奈格里亚原虫的第一份报告

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2022-02-14 DOI: 10.4467/16890027ap.21.005.15379

Abida Akbar, Abdul Hameed, A. Alouffi, Mashal M. Almutairi, T. Tanveer, A. Matin

{"title":"First report of successful Naegleria detection from environmental resources of some selected areas of Rawlakot, Azad Jammu and Kashmir, Pakistan","authors":"Abida Akbar, Abdul Hameed, A. Alouffi, Mashal M. Almutairi, T. Tanveer, A. Matin","doi":"10.4467/16890027ap.21.005.15379","DOIUrl":"https://doi.org/10.4467/16890027ap.21.005.15379","url":null,"abstract":"Naegleria belongs to the free-living amoeba family and is well-known as a human pathogen. It is recognized as etiological agent of primary amoebic meningoencephalitis involving central nervous system which always leads to death. To date, there is not a single report demonstrating Naegleria isolation and identification from environmental sources of Rawlakot, Azad Jammu and Kashmir Pakistan, and thus the aim of this study. Naegleria was isolated on non-nutrient agar plates seeded with heat killed E. coli and confirmed by morphological properties of the both stages of cyst or trophozoites. Furthermore, PCR was conducted along with direct sequencing of the PCR product for molecular identification. PCR and sequencing data verified the amplification of Naegleria sp. (07) and Vahlkampfia sp. (01) from both water and soil samples. Interestingly two species were successfully isolated and cultured on both 30 and 45°C. To the best of our knowledge this is the first report demonstrating the Naegleria isolation and molecular characterization from environmental sources of Rawlakot, Azad Jammu and Kashmir, Pakistan. The author is anxious for further evaluation of the pathogenic potential of the identified species and explores drinking water across Pakistan to investigate its quality and frequency of FLA, which might be a possible human hazard in future.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46930120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Future prospects for investigating ciliate biodiversity 研究纤毛虫生物多样性的未来展望

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2022-01-01 DOI: 10.4467/16890027ap.22.005.16236

Ľ. Rajter, Borong Lu, Erika Rassoshanska, M. Dunthorn

引用次数: 1

Testate amoebae: a review on their multiple uses as bioindicators 遗存变形虫:作为生物指示物的多种用途综述

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2022-01-01 DOI: 10.4467/16890027ap.22.001.15671

Yasmin de Góes Cohn Freitas, Beatriz Rodrigues D’Oliveira Ramos, Yemna Gomes da Silva, Gabriela Silva Sampaio, Letícia da Silva Nascimento, C. Branco, V. Miranda

{"title":"Testate amoebae: a review on their multiple uses as bioindicators","authors":"Yasmin de Góes Cohn Freitas, Beatriz Rodrigues D’Oliveira Ramos, Yemna Gomes da Silva, Gabriela Silva Sampaio, Letícia da Silva Nascimento, C. Branco, V. Miranda","doi":"10.4467/16890027ap.22.001.15671","DOIUrl":"https://doi.org/10.4467/16890027ap.22.001.15671","url":null,"abstract":"Testate amoebae (TA) are unicellular protozoans enclosed in a test capable of indicating a wide variety of environmental conditions. Among others, characteristics such as short life cycle, great sensitivity and worldwide distribution makes them adequate bioindicators. As a complement to physical and chemical measurements, biomonitoring can be a cheaper and fastest way of environmental monitoring. This research sought to evaluate the extent of TA use in biomonitoring and the responses given by them to environmental features. The research was conducted in Scielo, Science Direct, Online Library, Google Scholar and Capes Journal Portal and yielded 211 papers. TA bioindication is able to provide information on metal, trace element and atmospheric pollution, and to point out different trophic states, pH, and evidence on characteristics of hydrology. Further, TA can be used in paleoenvironmental reconstruction as they reflect climate, volcanic and even sea level change phenomena. Sometimes, together with other organisms in environmental analysis, they have shown to be an important complement to biomonitoring. Additionally, a functional traits approach has been recently included as a promising tool. Methodological adjustments that have been conducted throughout the years are allowing TA use to be more reliable and precise. This review provides insight on the many possible functions of TA in bioindication studies, highlighting their wide use as bioindicators.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70978586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Anti-cryptosporidial activity of Camellia sinensis (green tea extract) in experimentally infected immunocompromised mice 茶树提取物对免疫功能低下小鼠的抗隐孢子虫活性

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2022-01-01 DOI: 10.4467/16890027ap.22.002.16205

E. S. El-Wakil, Eman Ali Mohamed, E. El-wakil, T. Aboushousha, Neimat M Amer

{"title":"Anti-cryptosporidial activity of Camellia sinensis (green tea extract) in experimentally infected immunocompromised mice","authors":"E. S. El-Wakil, Eman Ali Mohamed, E. El-wakil, T. Aboushousha, Neimat M Amer","doi":"10.4467/16890027ap.22.002.16205","DOIUrl":"https://doi.org/10.4467/16890027ap.22.002.16205","url":null,"abstract":"Cryptosporidium parvum, an Apicomplexan parasite, is an important cause of diarrheal disease,especially in immunodeficient hosts. Nevertheless, there is no entirely successful therapeutic agent against cryptosporidiosis to date. Hence, this study aims to test the potential prophylactic and therapeutic effect of Camellia sinensis (green tea extract) in dexamethasone immunosuppressed mice versus the nowadays used drug, Nitazoxanide (NTZ).Parasitological and molecular methods were used to characterize Cryptosporidium oocysts before infection. Fifty bred femaleSwissAlbino mice were divided into 5 groups; group I (GI)(GTP):immunosuppressed and prophylactically treated with green tea extractfor 5 days prior to infection,group II (GII)(GTT):immunosuppressed, infected with Cryptosporidium parvum and treated with green tea extract, group III (GIII)(NT): immunosuppressed, infected and treated with NTZ, group IV (GIV)(PC): immunosuppressed and infected (Positive control), group V (GV)(NC): immunosuppressed and non-infected (Negative control).Furthermore, parasitological examination for oocysts in the stool, and histopathological examination for the small intestine and liver specimens were performed for the study groups.Cryptosporidium oocysts used for induction of infection proved to beCryptosporidium parvumgenotype 2. Moreover, a significant oocyst reduction in fecal samples correlated with an improvement of histopathological changesin the small intestinal and liver tissues in GI(GTP), GII(GTT)and GIII(NT) groups. Besides, the GII(GTT)group showed the best improvement inparasitological andhistopathological parametersamong the test groups.This study revealed that Camellia sinensis(green tea extract) has potential activity against cryptosporidiosis and could serve as a promising prophylactic and therapeutic anti-cryptosporidial agent.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70978637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A Multi-year Seasonal Study of Amoeboid Protists in Surface Water at the Margin of a Hudson River Estuary Salt Marsh 哈德逊河口盐沼边缘地表水中变形虫原生生物的多年季节性研究

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2022-01-01 DOI: 10.4467/16890027ap.22.007.16990

O. Anderson

{"title":"A Multi-year Seasonal Study of Amoeboid Protists in Surface Water at the Margin of a Hudson River Estuary Salt Marsh","authors":"O. Anderson","doi":"10.4467/16890027ap.22.007.16990","DOIUrl":"https://doi.org/10.4467/16890027ap.22.007.16990","url":null,"abstract":"Marshes bordering rivers and estuaries are productive ecosystems that interact dynamically with the adjacent water mass. This is a multi-year study (2019-2022) of seasonal changes in the density of naked amoebae in monthly samples from the surface water of the Hudson estuary near Piermont, N. Y. with relationships to key environmental variables (surface water temperature, salinity, Secchi depth representing turbidity, and enterococcus bacterial counts). During the colder months (November to March), when decayed leaves and litter from the deciduous marsh grass produced organic matter in the sediment surface, the mean abundance of active amoebae ± standard error of the mean (SEM) was higher (3.07 ± 0.99 x 104/ L, N = 7). In warmer months (May to September) the abundance of amoebae was lower (1.35 ± 0.29 x 104 / L, N = 10). A multivariate linear regression analysis was performed relating amoeba abundance to four major water mass variables, resulting in the following statistically significant equation (p = 0.03): AD = 0.121*T + 0.301*L – 0.047*S + 0.359 * C, where: AD = active amoebae density (x 104/L), T = temperature (oC), L = tide level (m), S = Secchi disc depth (cm) and C = bacterial enterococcus concentration (number/ml). In general, given the increasing evidence of the potential importance of amoeboid protists in aquatic ecosystems, further research is warranted on their role in food webs and the carbon biogeochemical cycle within heterotrophic estuarine and coastal waters.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"26 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70978915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advances in Application of Transcriptomics: Research onHeterotrophic and Autotrophic Protists 异养和自养原生生物转录组学研究进展

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2022-01-01 DOI: 10.4467/16890027ap.22.003.16206

引用次数: 1

Free-living Heterotrophic Flagellates (Protista) from Two Hypersaline Lakes in Turkey 土耳其两个高盐湖的自由生活异养鞭毛虫(原生鞭毛虫)

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2022-01-01 DOI: 10.4467/16890027ap.22.008.17111

E. Aydin, W. Lee

引用次数: 0

Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics 包囊过程中水溶性蛋白的双向电泳分析及细胞骨架动力学

IF 0.5 4区生物学

Acta Protozoologica Pub Date : 2021-03-01 DOI: 10.4467/16890027AP.20.009.13264

Yoichiro Sogame, K. Kojima, T. Takeshita, Shiho Kikuchi, Yuto Shimada, Rikiya Nakamura, M. Arikawa, S. Miyata, E. Kinoshita, F. Suizu, T. Matsuoka

{"title":"Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics","authors":"Yoichiro Sogame, K. Kojima, T. Takeshita, Shiho Kikuchi, Yuto Shimada, Rikiya Nakamura, M. Arikawa, S. Miyata, E. Kinoshita, F. Suizu, T. Matsuoka","doi":"10.4467/16890027AP.20.009.13264","DOIUrl":"https://doi.org/10.4467/16890027AP.20.009.13264","url":null,"abstract":"Assays of protein contained in water-soluble fraction of encysting cells Colpoda cucullus Nag-1 by two-dimensional electrophoresis (2-D PAGE) and mass spectrometry (MS) revealed that the amount of β-tubulin abruptly increased in 2.5–10 h after encystment induction. Judging from the results that total α-tubulin content did not decrease much until 12 h after encystment induction, the result indicates that disassembly of microtubules may occur soon after encystment is induced. Therefore, we tried to visualize dynamics of microtubules. Immunofluorescence microscopy using anti-α-tubulin antibody indicated that disassembly of axonemal microtubules of cilia became within 1.5 h after encystment induction, and resorbed in 3 days. Although the cytoplasmic microtubules failed to be visualized clearly, encystmentdependent globulation of cells was promoted by taxol, an inhibitor of disassembly of microtubules. It is possible that a temporary formation of cytoplasmic microtubules may be involved in cell globulation.\u0000\u0000The phosphorylation level of actin (43 kDa) became slightly elevated just after encystment induction. Lepidosomes, the sticky small globes surrounding encysting cells, were vividly stained with Acti-stain 555 phalloidin, suggesting that 43-kDa actin or its homologues may be contained in lepidosomes.","PeriodicalId":50883,"journal":{"name":"Acta Protozoologica","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42167271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2