环介导等温扩增(LAMP)和超敏PCR在沙特阿拉伯西部疟疾诊断中的应用

IF 1.5 4区 生物学 Q4 MICROBIOLOGY
Rasha H. Solimam, A. Martín-Ramírez, J. Rubio, E. Khalifa, Bushra E. Hussein, Maha M. Wahab, M. Lanza, Yousry A. Hawash
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引用次数: 0

摘要

疟疾诊断仍然是控制周期中最重要的步骤之一,特别是在寄生虫感染率低的流行国家。环介导等温扩增(LAMP)和超灵敏PCR(Us-PCR)是两种很有前途的疟疾诊断候选者。Taif KSA费萨尔国王医院进行的一项横断面研究涉及患有疟疾迹象和症状的患者,选择35份通过嵌套多重PCR作为参考方法诊断的血液样本(13份恶性疟原虫、17份间日疟原虫、3份混合恶性疟原虫和间日疟原虫)和两份阴性对照纳入本研究,以分析两种LAMP方法(LAMP OptiGene®和LAMP WarmStart®)和两种超灵敏PCR(Us PCR TARE-2和Us PCR Var-ATS)的性能。LAMP OptiGene®和LAMP WarmStart®对恶性疟原虫的性能与Us PCR TARE 2和Us PCR var ATS的性能相同且更好,分别达到93.75%的灵敏度、100%的特异性和97.17%的准确率,而Us PCR TARE 2和81.25%的灵敏度、100%的特异性和94.29%的准确率,特异性94.74%,准确率88.57%。在间日疟原虫的诊断中,LAMP OptiGene®表现出色,具有100%的敏感性、特异性和准确性,而LAMP WarmStart®和Us PCR Cox1实现了100%的灵敏度、特异性93.33%和97.14%的准确性。研究结果强调了在不同环境中使用LAMP技术进行疟疾现场诊断的好处,在不同环境下,必须需要更敏感和可靠的分子工具,但同时消除了高成本、长周转时间和需要训练有素的高度专业化技术人员来执行更复杂的分子技术的问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Diagnostic performance of loop-mediated isothermal amplification (LAMP) and Ultra-sensitive PCR in diagnosis of malaria in western Saudi Arabia
Malaria diagnosis continues to be one of the most important steps in the cycle of control specially in endemic countries with low parasitic load infections. Loop-mediated isothermal amplification (LAMP) and ultrasensitive PCR (Us-PCR) are two promising candidates for malaria diagnosis. A cross sectional study performed at King Faisal Hospital, Taif KSA involved patients suffering from signs and symptoms suggesting of malaria, 35 blood samples diagnosed by Nested Multiplex PCR as a reference method (13 P. falciparum, 17 P. vivax, 3 mixed P. falciparum and P. vivax) plus two negative controls were selected to be included in this study to analyse the performance of two LAMP methods (LAMP OptiGene® and LAMP WarmStart®) and two ultrasensitive PCRs (Us-PCR TARE-2 and Us-PCR Var-ATS). LAMP OptiGene® and LAMP WarmStart® performances were identical and better than the performance of Us PCR TARE 2 and Us-PCR var-ATS for P. falciparum, achieving 93.75% sensitivity, 100% specificity and 97.17% accuracy versus 87.5% sensitivity, 100% specificity and 94.29% accuracy for the Us PCR TARE 2 and 81.25% sensitivity, 94.74% specificity and 88.57% accuracy for the Us PCR var-ATS respectively. In P. vivax diagnosis LAMP OptiGene® performed excellently with 100% sensitivity, specificity, and accuracy while LAMP WarmStart® and Us-PCR Cox1 achieved 100% sensitivity, specificity 93.33% and 97.14% accuracy. The study results highlighted the benefits of using LAMP techniques for field diagnosis of malaria in different settings where the need for a more sensitive and reliable molecular tool is mandatory but at the same time removing the high cost, long turnaround time and the need of highly specialized trained technicians to perform more sophisticated molecular techniques.
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来源期刊
Acta Protozoologica
Acta Protozoologica 生物-微生物学
CiteScore
2.00
自引率
0.00%
发文量
8
审稿时长
>12 weeks
期刊介绍: Acta Protozoologica - International Journal on Protistology - is a quarterly journal that publishes current and comprehensive, experimental, and theoretical contributions across the breadth of protistology, and cell biology of Eukaryote microorganisms including: behaviour, biochemistry and molecular biology, development, ecology, genetics, parasitology, physiology, photobiology, systematics and phylogeny, and ultrastructure. It publishes original research reports, critical reviews of current research written by invited experts in the field, short communications, book reviews, and letters to the Editor.
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