Advances in Biophysics最新文献_第5页

Brain neurosteroids are 4th generation neuromessengers in the brain: Cell biophysical analysis of steroid signal transduction 脑神经类固醇是大脑中的第四代神经信使:类固醇信号转导的细胞生物物理分析

Advances in Biophysics Pub Date : 2003-01-01 DOI: 10.1016/S0065-227X(03)80002-3

Suguru Kawato , Makoto Yamada , Tetsuya Kimoto

引用次数: 54

To the memory of Mr. Miyazaki 为了纪念宫崎骏先生

Advances in Biophysics Pub Date : 2003-01-01 DOI: 10.1016/S0065-227X(03)80007-2

引用次数: 0

Induced potential model of muscular contraction mechanism and myosin molecular structure 肌收缩的诱导电位模型及肌球蛋白分子结构

Advances in Biophysics Pub Date : 1999-01-01 DOI: 10.1016/S0065-227X(99)80006-9

Toshio Mitsui

{"title":"Induced potential model of muscular contraction mechanism and myosin molecular structure","authors":"Toshio Mitsui","doi":"10.1016/S0065-227X(99)80006-9","DOIUrl":"10.1016/S0065-227X(99)80006-9","url":null,"abstract":"<div>The proposed model is characterized by the constant r (Eq. 2-1), the induced potential (Fig. 1), two attached states of a myosin head (Fig. 1), the nonlinear elastic property of the crossbridge (Eq. 2-7), and the expression of <math><mtext>U</mtext><msup><mi></mi><mn>∗</mn></msup></math> (Eqs. 3-8 and 3-9), which led us to the following conclusions. <ul><li>1.1. The following various magnitudes of myosin head motion are compatible with each other: about 2 nm of the quantity called power stroke by Irving (27), which is the mean moving distance of myosin head in the isometric tension in our model, 4–5 nm of the displacement of a single myosin head during one ATP hydrolysis cycle (Molloy et al. (20)) or a few tens of nm when the actin and myosin filaments are set parallel (Tanaka et al. (21) and Kitamura et al. (42)), and more than 200 nm of the myosin head displacement in a multi-myosin head system below 22 °C (Harada et al. (19)).</li><li>2.2. There is one-to-one coupling between the ATP hydrolysis cycle and the attachment-detachment cycle of a myosin head in accordance with the generally accepted concept of chemical reactions, since the head is trapped in the spatially shifting wide potential well (Fig. 1) until εATP is exhausted. Here, an actin filament interacts with a myosin head like a single molecule.</li><li>3.3. The calculated tension dependence of muscle stiffness agrees well with the observations by Ford et al. (12), as shown in Fig. 9.</li><li>4.4. The calculated shortening velocity V of muscle as a function of <math><mtext>P</mtext><mtext>P</mtext><msub><mi></mi><mn>0</mn></msub></math> agreed very well with experimental results as shown in Fig. 13. The deviation from the Hill equation (34) observed by Edman (32) is related with <math><mtext>U</mtext><msup><mi></mi><mn>∗</mn></msup></math> being effectively infinite for fJ < κbyc0 (Fig. 10).</li><li>5.5. Calculated energy liberation rate W + H as a function of <math><mtext>P</mtext><mtext>P</mtext><msub><mi></mi><mn>0</mn></msub></math> has characteristics almost the same as the Hill equation (33), and agrees well with the experimental results as shown in Fig. 14.</li><li>6.6. The time course of tension recovery after a quick length change is determined by four parameters: κf, κb, a, and Z0. Among them, κf, κb (Eq. 2–22) and a (Eq. 4-21) are readily determined by analysis of the steady filame","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"36 ","pages":"Pages 107-158"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0065-227X(99)80006-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21327135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Contributor sketches 贡献者草图

Advances in Biophysics Pub Date : 1999-01-01 DOI: 10.1016/S0065-227X(99)90000-X

引用次数: 0

Evolution of the carabid ground beetles 瓢虫的进化

Advances in Biophysics Pub Date : 1999-01-01 DOI: 10.1016/S0065-227X(99)80005-7

Syozo Osawa , Zhi-Hui Su , Choonggon Kim , Munehiro Okamoto , Osamu Tominaga , Yûki Imura

引用次数: 23

Suggestions to authors 给作者的建议

Advances in Biophysics Pub Date : 1999-01-01 DOI: 10.1016/S0065-227X(99)90001-1

引用次数: 0

Biophysical studies on ATP synthase ATP合酶的生物物理研究

Advances in Biophysics Pub Date : 1999-01-01 DOI: 10.1016/S0065-227X(99)80003-3

Yasuo Kagawa

{"title":"Biophysical studies on ATP synthase","authors":"Yasuo Kagawa","doi":"10.1016/S0065-227X(99)80003-3","DOIUrl":"10.1016/S0065-227X(99)80003-3","url":null,"abstract":"<div>The isolation of ATP synthase (F0F1) (82) and F0 (83) 34 years ago finally revealed that F0F1 is a motor composed of F0 (ion-motor, abc subunits) and F1 (ATP-motor, α3β3γδε subunits) (Fig. 1). The single molecule videotape (4, 5, 65, 66) revealed that γε axis of F1 rotates counterclockwise, proceeds by each <math><mtext>2π</mtext><mtext>3</mtext></math> step, and is driven by torque of 42 pN·nm (12) with nearly 100% efficiency (5) (Fig. 4). The motor is composed of a rotor (γε-F0-c) and a stator (α3β3δ-F0-ab), and the rotor is connected to a shaft (γε). Since F0F1 is driven by Δ<math><mtext></mtext><mtext>̄</mtext></math>gmH+ (9, 10, 84), biophysical studies on stable TF0F1 (1, 7) are essential to elucidate the mechanism. These include nanomechanics (4, 5) (Fig. 4), crystallography (2, 3) (Figs. 2 and 3), NMR (51, 52), ESR (56), synchrotron analysis (3, 28), and electrophysiology (10, 25). The KmATP value of rotation is 0.8 μm, with the Vmax of 3.9 rps (5). This corresponds to the bi-site catalysis in proton transport by F0F1 (10, 70, 84). X-ray crystallography of MF1 (2) and the α3β3 oligomer of TF1 (3) (Fig. 2) together with mutation analyses revealed the role of residues in the rotation. The idea of elastic energy store is proposed in α3β3γ during the stepping time (up to a few sec) after the ATP binding. Biological studies have partially clarified the genetic and kinetic regulation of the rotation in MF1. Both theories (6, 7, 62, 64, 85) and the biological significance (17) of the intramolecular rotation of F0F1 await further studies, especially those of F0 and minor subunits.</div>","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"36 ","pages":"Pages 1-25"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0065-227X(99)80003-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21327132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Notice to authors 作者须知

Advances in Biophysics Pub Date : 1999-01-01 DOI: 10.1016/S0065-227X(99)80002-1

引用次数: 0

Multiple sequence alignment: Algorithms and applications 多序列比对:算法与应用

Advances in Biophysics Pub Date : 1999-01-01 DOI: 10.1016/S0065-227X(99)80007-0

Osamu Gotoh

{"title":"Multiple sequence alignment: Algorithms and applications","authors":"Osamu Gotoh","doi":"10.1016/S0065-227X(99)80007-0","DOIUrl":"10.1016/S0065-227X(99)80007-0","url":null,"abstract":"<div>Elucidation of interrelationships among sequence, structure, function, and evolution (FESS relationships) of a family of genes or gene products is a central theme of modern molecular biology. Multiple sequence alignment has been proven to be a powerful tool for many fields of studies such as phylogenetic reconstruction, illumination of functionally important regions, and prediction of higher order structures of proteins and RNAs. However, it is far too trivial to automatically construct a multiple alignment from a set of related sequences. A variety of methods for solving this computationally difficult problem are reviewed. Several important applications of multiple alignment for elucidation of the FESS relationships are also discussed.For a long period, progressive methods have been the only practical means to solve a multiple alignment problem of appreciable size. This situation is now changing with the development of new techniques including several classes of iterative methods. Today's progress in multiple sequence alignment methods has been made by the multidisciplinary endeavors of mathematicians, computer scientists, and biologists in various fields including biophysicists in particular. The ideas are also originated from various backgrounds, pure algorithmics, statistics, thermodynamics, and others. The outcomes are now enjoyed by researchers in many fields of biological sciences.In the near future, generalized multiple alignment may play a central role in studies of FESS relationships. The organized mixture of knowledge from multiple fields will ferment to develop fruitful results which would be hard to obtain within each area. I hope this review provides a useful information resource for future development of theory and practice in this rapidly expanding area of bioinformatics.</div>","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"36 ","pages":"Pages 159-206"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0065-227X(99)80007-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21327136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 102

Ryanodine receptor isoforms in excitation-contraction coupling Ryanodine受体在兴奋-收缩耦合中的异构体

Advances in Biophysics Pub Date : 1999-01-01 DOI: 10.1016/S0065-227X(99)80004-5

Yasuo Ogawa, Nagomi Kurebayashi, Takashi Murayama

{"title":"Ryanodine receptor isoforms in excitation-contraction coupling","authors":"Yasuo Ogawa, Nagomi Kurebayashi, Takashi Murayama","doi":"10.1016/S0065-227X(99)80004-5","DOIUrl":"10.1016/S0065-227X(99)80004-5","url":null,"abstract":"<div>Three genomically distinct isoforms of RyR are now known. RyR1 homologue is the primary isoform in skeletal muscles, whereas in cardiac muscles it is RyR2 homologue. RyR3 homologue occurs ubiquitously in many cells, but the biological function is little known, partly because of its minuscule amount in mammalian cells. The difference among RyR isoforms may not be so great in CICR activity, in other words, in the interaction of RyR isoforms with Ca2+, adenine nucleotides and caffeine. Species specificity among RyR1 homologues may be more important in the apparent difference between RyR1 and RyR3 homologues. CICR is likely to be the dominant underlying mechanism for E-C coupling in the cardiac muscle and probably in cells other than the skeletal muscle where the significance of CICR is controversial in physiological contraction. In E-C coupling of skeletal muscle (DICR), the reciprocal tight interactions between DHPR and RyR1 are critically required. The α1 subunit of DHPR was only the main target of our current interests in the interaction with RyR1; the involvement of auxiliary subunits of <math><mtext>α</mtext><msub><mi></mi><mn>2</mn></msub><mtext>δ</mtext></math> and β subunits and their mutual interactions, however, are also important. DICR and CICR in RyR1 share common properties of stimulation by concentrated solutes and modulation by luminal calcium or Ca2+, suggesting that the main difference between the two Ca2+ release mechanisms may be in the gating mechanism of the channel. Further investigations are required to understand molecular interactions during E-C coupling.</div>","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"36 ","pages":"Pages 27-64"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0065-227X(99)80004-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21327133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 79