PhytoFrontiers™最新文献_第4页

Disease Progress and Detection of a California Resistance-Breaking Strain of Tomato Spotted Wilt Virus in Tomato with LAMP and CRISPR-Cas12a Assays 利用 LAMP 和 CRISPR-Cas12a 检测番茄斑点萎蔫病病毒在番茄中的病害进展和加州抗性突破菌株

PhytoFrontiers™ Pub Date : 2024-01-24 DOI: 10.1094/phytofr-05-23-0058-fi

Tatsiana Shymanovich, A. Saville, Noor Mohammad, Qingshan Wei, David Rasmussen, Kirsten A. Lahre, D. Rotenberg, A. Whitfield, J. Ristaino

{"title":"Disease Progress and Detection of a California Resistance-Breaking Strain of Tomato Spotted Wilt Virus in Tomato with LAMP and CRISPR-Cas12a Assays","authors":"Tatsiana Shymanovich, A. Saville, Noor Mohammad, Qingshan Wei, David Rasmussen, Kirsten A. Lahre, D. Rotenberg, A. Whitfield, J. Ristaino","doi":"10.1094/phytofr-05-23-0058-fi","DOIUrl":"https://doi.org/10.1094/phytofr-05-23-0058-fi","url":null,"abstract":"Use of tomato cultivars with the Sw-5 resistance gene cluster has led to the occurrence of resistance-breaking (RB) tomato spotted wilt virus (TSWV) strains globally, including California and, recently, North Carolina and Texas. We documented disease on tomato infected with either an RB strain from California (CA-RB) or a wild type (CA-WT) strain of TSWV on tomato with (cultivar Mountain Merit) or without (cultivar Mountain Fresh Plus) the Sw-5b resistance gene and detected virus incidence over time using microneedle RNA extractions and LAMP. We developed a LAMP/Cas12a assay for detection of the CA-C118Y mutation in a CA-RB strain and tested the assay with field samples. Disease in the susceptible cultivar was less severe with CA-RB than with the CA-WT strain. In contrast, the resistant cultivar had little disease when inoculated with the CA-WT strain but exhibited stunting of greater than 50% when inoculated with the CA-RB strain. In the susceptible tomatoes, the detection rates over time by LAMP reaction were higher in CA-WT than in CA-RB-inoculated plants. In resistant tomato, CA-RB remained detectable by TSWV LAMP over 14 days, whereas the WT strain was undetectable. A two-step LAMP/Cas12a assay differentiated the two strains in 1 h. Our methods were validated with samples from TSWV-infected North Carolina fields. A phylogeny of NSm gene sequences that included North Carolina field samples revealed two independent origins of the North Carolina RB isolates. The LAMP/Cas12 assay showed excellent detection of the CA-C118Y mutation. The TSWV LAMP/Cas12a assay is adaptable for in-field applications on either a smart phone platform or heat block. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":"54 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139602116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-Genome Sequence of Colletotrichum brevisporum Causing Anthracnose on Papaya (Carica papaya) 引起木瓜炭疽病的 Colletotrichum brevisporum 的全基因组序列

PhytoFrontiers™ Pub Date : 2024-01-17 DOI: 10.1094/phytofr-07-23-0080-a

Min Yang, Chenping Zhou, R. Kuang, Xiaming Wu, Yu-Si Wei

{"title":"Whole-Genome Sequence of Colletotrichum brevisporum Causing Anthracnose on Papaya (Carica papaya)","authors":"Min Yang, Chenping Zhou, R. Kuang, Xiaming Wu, Yu-Si Wei","doi":"10.1094/phytofr-07-23-0080-a","DOIUrl":"https://doi.org/10.1094/phytofr-07-23-0080-a","url":null,"abstract":"Colletotrichum spp. are the causal agents of papaya anthracnose, which seriously affects the quality and economic value of papaya fruits. Among them, Colletotrichum brevisporum is a new and important causal pathogen of anthracnose in papaya. The infection mechanisms of C. brevisporum are still unclear, and the genome sequence of C. brevisporum has not been released. In order to systemically explore the interaction between papaya and C. brevisporum, we sequenced the whole genome of the C. brevisporum strain C1, which was isolated from an infected papaya fruit in Guangdong Province, China. The assembly consists of 18 scaffolds with a genome size of 62.66 Mb. Furthermore, we identified genes that may be associated with pathogenicity, such as carbohydrate-active enzymes, secreted proteins, and secondary metabolite gene clusters. This genome resource will provide a valuable resource for future studies on the pathogenesis of C. brevisporum and comparative genomic analyses of the Colletotrichum genus. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139616953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete Genome Resource of a Fungal Antagonistic Strain: Pseudomonas tohonis 真菌拮抗菌株的完整基因组资源：陶氏假单胞菌

PhytoFrontiers™ Pub Date : 2024-01-12 DOI: 10.1094/phytofr-09-23-0124-a

Wan-Zhen Luo, Hengguang Zhu, Hong-Yue Qi, Jiangqing Song, Dan-Dan Zhang, X. Dai, Mai-He-Mu-Ti Mijiti, Jiemin Chen, Dongfei Han, Dan Wang

引用次数: 0

Phthalic Acid and Its Role in Roots of Melon Plants (Cucumis melo) 邻苯二甲酸及其在甜瓜（Cucumis melo）根中的作用

PhytoFrontiers™ Pub Date : 2024-01-12 DOI: 10.1094/phytofr-04-23-0046-r

S. Marquez, Kevin M. Crosby, Bhimanagouda S. Patil, Amir M. H. Ibrahim, Carlos A. Avila, H. Pessoa, Jashbir Singh

{"title":"Phthalic Acid and Its Role in Roots of Melon Plants (Cucumis melo)","authors":"S. Marquez, Kevin M. Crosby, Bhimanagouda S. Patil, Amir M. H. Ibrahim, Carlos A. Avila, H. Pessoa, Jashbir Singh","doi":"10.1094/phytofr-04-23-0046-r","DOIUrl":"https://doi.org/10.1094/phytofr-04-23-0046-r","url":null,"abstract":"Melon is an important crop worldwide. However, this crop has many problems, such as high production costs, pests, diseases, and sensitivity to abiotic stresses. Among the diseases that negatively impact its production, vine decline disease (VDD), caused by the fungus Monosporascus cannonballus, is very dangerous because it affects the plant when the fruit is almost ready to be harvested, which causes huge losses to growers. In addition, this disease is widely spread in areas where melons are grown all over the world, and its control through soil fumigation is costly and polluting. Thus, cheap and sustainable ways to combat it must be found. Therefore, this study was conducted with the objectives of identifying and quantifying phenolic compounds produced during the interaction of melon plants with the fungus M. cannonballus, which can be useful in controlling VDD, ameliorating cultural practices of this crop, and consequently reducing production costs. Two varieties were grown and inoculated with the fungus M. cannonballus: TAM-Uvalde (susceptible) and USDA PI 124104 (resistant). Their roots were sampled before inoculating with the fungus (0 h) and 24, 48, and 72 h after inoculation. The root chemicals were then analyzed using high-performance liquid chromatography. Our results indicate that phthalic acid was induced in the roots after inoculation in both varieties, which suggests that it is produced by the plant as a defensive compound. Also, the production of phthalic acid varied over time according to the variety used, which suggests its allelopathic function. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":"47 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139533011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete Genome Resource of Xanthomonas oryzae pv. leersiae Strain YXol-1 Isolated From Plateau Japonica Rice in Yunnan Province, China 从中国云南省高原粳稻中分离出的黄单胞菌（Xanthomonas oryzae pv. leersiae）菌株 YXol-1 的完整基因组资源

PhytoFrontiers™ Pub Date : 2024-01-03 DOI: 10.1094/phytofr-10-23-0139-a

X. A, Le Mei, Yayun Yang, Cui-feng Tang, Feifei Zhang, Chao Dong, Bin Wang, Pengcheng Liu, Xifeng Chen, Bojun Ma, Luyuan Dai

引用次数: 0

Genome Resource of Bacillus subtilis KRS015, a Potential Biocontrol Agent for Verticillium dahliae 枯草芽孢杆菌 KRS015--一种潜在的大丽轮枝菌生物控制剂--的基因组资源

PhytoFrontiers™ Pub Date : 2023-12-17 DOI: 10.1094/phytofr-08-23-0114-a

Jiangqing Song, Jiemin Chen, Dan-Dan Zhang, Ran Li, Zhiqiang Kong, Hengguang Zhu, X. Dai, Dongfei Han, Dan Wang

引用次数: 0

Phenotypic fungicide resistance and cross-resistance among Nothopassalora personata populations Nothopassalora personata 种群对杀真菌剂的表型抗性和交叉抗性

PhytoFrontiers™ Pub Date : 2023-12-11 DOI: 10.1094/phytofr-10-23-0137-r

Hope Renfroe-Becton, Jonathan Croft, Charles Davis, Joe Varn, Albert Culbreath, David Langston, Dan Anco

{"title":"Phenotypic fungicide resistance and cross-resistance among Nothopassalora personata populations","authors":"Hope Renfroe-Becton, Jonathan Croft, Charles Davis, Joe Varn, Albert Culbreath, David Langston, Dan Anco","doi":"10.1094/phytofr-10-23-0137-r","DOIUrl":"https://doi.org/10.1094/phytofr-10-23-0137-r","url":null,"abstract":"Late leaf spot, caused by Nothopassalora personata, is an economically important disease of peanut which routinely requires preventative fungicide application. The objectives of this study were to quantify the risk of phenotypic fungicide resistance and potential cross-resistance among Nothopassalora personata populations. A total of 59 samples of N. personata isolates were collected from 28 grower or research fields in SC, GA, and VA. Phenotypic resistance of isolates was evaluated against 14 fungicides and a nontreated control, with active ingredients from the quinone outside inhibitor (QoI), demethylation inhibitor (DMI), and succinate-dehydrogenase inhibitor (SDHI) classes. Overall, lesion development ≥ 15% was observed with nearly all isolates for at least one of the active ingredients tested. Correlations between active ingredients, penthiopyrad and pydiflumetofen, as well as bixafen plus flutriafol and pydiflumetofen, and their lesion development risk indicates potential N. personata populations with SDHI cross-resistance. While these data do not confirm the presence of genetic resistance markers, results paired with observed reduced efficacy in the field reinforce the strong need for an integrated approach in managing late leaf spot. In areas with high probabilities for resistance to several modes of action, longer rotations out of peanut, varieties with greater levels of resistance, earlier planting, and continued incorporation of active ingredients with multi-site mode of action in fungicide programs are advised for preserving fungicide efficacy or reducing selection pressure.","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":"71 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139183475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome sequence resource of a taro bacterial soft rot pathogen Dickeya fangzhongdai ZXC1 芋头细菌软腐病病原体 Dickeya fangzhongdai ZXC1 的基因组序列资源

PhytoFrontiers™ Pub Date : 2023-12-11 DOI: 10.1094/phytofr-07-23-0090-a

Zhongqiao Chen, Weihan Gu, Congcong Xie, Huagui Gao, Shufen Huang, Lian-hui Zhang, L. Liao

{"title":"Genome sequence resource of a taro bacterial soft rot pathogen Dickeya fangzhongdai ZXC1","authors":"Zhongqiao Chen, Weihan Gu, Congcong Xie, Huagui Gao, Shufen Huang, Lian-hui Zhang, L. Liao","doi":"10.1094/phytofr-07-23-0090-a","DOIUrl":"https://doi.org/10.1094/phytofr-07-23-0090-a","url":null,"abstract":"Dickeya fangzhongdai ZXC1 is a newly identified highly virulent causal agent of taro bacterial soft rot disease isolated from the taro sample showing typical soft rot symptoms at Shaoguan, Guangdong Province, China. The pathogen produces plant cell wall degrading enzymes which macerate the taro tube tissues. In this study, we report the whole genome sequencing analysis of strain ZXC1. The results showed that strain ZXC1 has one circular DNA chromosome of 5,129,951 bp with 56.59% G+C content. In addition to sharing a conserved zms gene cluster that encodes the genes for biosynthesis of phytotoxin zeamines, and 11 copies of predicted pectate lyases genes, the strain ZXC1 genome contains more prophage loci and higher number of type IV secretion system (T4SS) and type IV secretion system (T6SS) gene clusters than the D. fangzhongdai strains isolated from pears or phalaenopsis, which may account for its strong virulence phenotype. The data from this study present a valuable resource for elucidation of the pathogenic mechanisms of D. fangzhongdai, and may aid in developing new disease control approaches to safeguard taro production.","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":"25 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139183959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Ralstonia pseudosolanacearum diversity and screening tomato, pepper, and eggplant resistance to manage bacterial wilt in South Asia 鉴定假丝酵母菌（Ralstonia pseudosolanacearum）的多样性，筛选番茄、辣椒和茄子的抗性，以管理南亚的细菌性枯萎病

PhytoFrontiers™ Pub Date : 2023-12-11 DOI: 10.1094/phytofr-10-23-0136-r

N. Subedi, Tabitha Cowell, Matthew L Cope-Arguello, P. Paul, G. Cellier, Hashem Bkayrat, Nicholas Bonagura, Agela Cadatal, Rachel Chen, Ariana Enriquez, Rama Parasar, Lisa Repetto, Aracely Hernandez Rivas, Mahnoor Shahbaz, Kaitlin White, Tiffany Lowe-Power, S. A. Miller

{"title":"Characterization of Ralstonia pseudosolanacearum diversity and screening tomato, pepper, and eggplant resistance to manage bacterial wilt in South Asia","authors":"N. Subedi, Tabitha Cowell, Matthew L Cope-Arguello, P. Paul, G. Cellier, Hashem Bkayrat, Nicholas Bonagura, Agela Cadatal, Rachel Chen, Ariana Enriquez, Rama Parasar, Lisa Repetto, Aracely Hernandez Rivas, Mahnoor Shahbaz, Kaitlin White, Tiffany Lowe-Power, S. A. Miller","doi":"10.1094/phytofr-10-23-0136-r","DOIUrl":"https://doi.org/10.1094/phytofr-10-23-0136-r","url":null,"abstract":"In South Asia, bacterial wilt pathogens in the Ralstonia solanacearum species complex (RSSC) impose major constraints on eggplant, tomato, and pepper production. To improve the efficacy of bacterial wilt management, the goals of this study were to (1) conduct a survey of RSSC pathogens in Bangladesh and Nepal, (2) characterize the genetic diversity of these isolates, and (3) screen 37 tomato, eggplant, and pepper accessions for resistance to six representative isolates from South Asia. We isolated 99 isolates from Bangladesh and 20 isolates from Nepal and determined that all are phylotype I isolates of the Ralstonia pseudosolanacearum species. We sequenced and assembled draft genomes for 25 isolates. Phylogenomic analyses suggest that there is a wide diversity of endemic phylotype I isolates in South Asia, and possible introductions of two clonal phylotype I lineages into Bangladesh and Nepal. We contextualize our newly described isolates based on prior reports of RSSC diversity in South Asia and global reports of RSSC pathogens on eggplant and pepper. Greenhouse trials revealed multiple tomato, eggplant, and pepper accessions that exhibit promising levels of resistance to six phylotype I isolates from South Asia.","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":"27 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139184047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene Expression Clusters Suggest Potential Mechanisms of Resistance to Powdery Mildew in Hop (Humulus lupulus) 基因表达集群揭示了啤酒花（Humulus lupulus）抵抗白粉病的潜在机制

PhytoFrontiers™ Pub Date : 2023-12-01 DOI: 10.1094/phytofr-09-22-0098-r

Renée L. Eriksen, Michele S. Wiseman, A. A. Magana, Nikhilesh Dhar, Ralph L. Reed, J. Fred Stevens, D. Gent, John A. Henning

{"title":"Gene Expression Clusters Suggest Potential Mechanisms of Resistance to Powdery Mildew in Hop (Humulus lupulus)","authors":"Renée L. Eriksen, Michele S. Wiseman, A. A. Magana, Nikhilesh Dhar, Ralph L. Reed, J. Fred Stevens, D. Gent, John A. Henning","doi":"10.1094/phytofr-09-22-0098-r","DOIUrl":"https://doi.org/10.1094/phytofr-09-22-0098-r","url":null,"abstract":"Powdery mildew, caused by Podosphaera macularis, is one of the most important diseases of hop in the Pacific Northwest of the United States. Resistant cultivars exist; however, recently, the pathogen has overcome two sources of resistance in popular cultivars. Breeding for new, emerging races of the pathogen is thus a continuing priority. We used RNA-seq to understand gene expression trends in a resistant (cv. USDA Nugget) and a susceptible (cv. Symphony) hop cultivar along a time series following inoculation. We found stronger transcriptional reprogramming in the resistant cultivar than the susceptible cultivar. Transcripts that increased in expression within 12 h post-inoculation in the resistant cultivar included genes in the jasmonic acid biosynthesis pathway, as well as phenylpropanoid biosynthesis. Transcripts that had a positive correlation with time post-inoculation in the resistant cultivar included genes involved in phosphorylation, cell-surface signaling pathways, and response to jasmonic acid. We used untargeted HPLC–HR-MS/MS analysis and confirmed an increase in jasmonic acid and several phenolic compounds. We hypothesize that resistance to powdery mildew in cv. Nugget may be at least partially due to perception of the pathogen outside of the cell, and transcriptional and metabolic reprogramming is mediated by jasmonic acid signaling. At least part of that transcriptional and metabolic reprogramming involves changes to lignin production, which might reinforce the cell wall. The multi-omics approach to describe the contrasting responses of a resistant and a susceptible hop cultivar following inoculation with this important pathogen may indicate new strategies for developing resistant germplasm and control methods. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":"17 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139187912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0