N. Subedi, Tabitha Cowell, Matthew L Cope-Arguello, P. Paul, G. Cellier, Hashem Bkayrat, Nicholas Bonagura, Agela Cadatal, Rachel Chen, Ariana Enriquez, Rama Parasar, Lisa Repetto, Aracely Hernandez Rivas, Mahnoor Shahbaz, Kaitlin White, Tiffany Lowe-Power, S. A. Miller
{"title":"鉴定假丝酵母菌(Ralstonia pseudosolanacearum)的多样性,筛选番茄、辣椒和茄子的抗性,以管理南亚的细菌性枯萎病","authors":"N. Subedi, Tabitha Cowell, Matthew L Cope-Arguello, P. Paul, G. Cellier, Hashem Bkayrat, Nicholas Bonagura, Agela Cadatal, Rachel Chen, Ariana Enriquez, Rama Parasar, Lisa Repetto, Aracely Hernandez Rivas, Mahnoor Shahbaz, Kaitlin White, Tiffany Lowe-Power, S. A. Miller","doi":"10.1094/phytofr-10-23-0136-r","DOIUrl":null,"url":null,"abstract":"In South Asia, bacterial wilt pathogens in the Ralstonia solanacearum species complex (RSSC) impose major constraints on eggplant, tomato, and pepper production. To improve the efficacy of bacterial wilt management, the goals of this study were to (1) conduct a survey of RSSC pathogens in Bangladesh and Nepal, (2) characterize the genetic diversity of these isolates, and (3) screen 37 tomato, eggplant, and pepper accessions for resistance to six representative isolates from South Asia. We isolated 99 isolates from Bangladesh and 20 isolates from Nepal and determined that all are phylotype I isolates of the Ralstonia pseudosolanacearum species. We sequenced and assembled draft genomes for 25 isolates. Phylogenomic analyses suggest that there is a wide diversity of endemic phylotype I isolates in South Asia, and possible introductions of two clonal phylotype I lineages into Bangladesh and Nepal. We contextualize our newly described isolates based on prior reports of RSSC diversity in South Asia and global reports of RSSC pathogens on eggplant and pepper. Greenhouse trials revealed multiple tomato, eggplant, and pepper accessions that exhibit promising levels of resistance to six phylotype I isolates from South Asia.","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":"27 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization of Ralstonia pseudosolanacearum diversity and screening tomato, pepper, and eggplant resistance to manage bacterial wilt in South Asia\",\"authors\":\"N. Subedi, Tabitha Cowell, Matthew L Cope-Arguello, P. Paul, G. Cellier, Hashem Bkayrat, Nicholas Bonagura, Agela Cadatal, Rachel Chen, Ariana Enriquez, Rama Parasar, Lisa Repetto, Aracely Hernandez Rivas, Mahnoor Shahbaz, Kaitlin White, Tiffany Lowe-Power, S. A. Miller\",\"doi\":\"10.1094/phytofr-10-23-0136-r\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In South Asia, bacterial wilt pathogens in the Ralstonia solanacearum species complex (RSSC) impose major constraints on eggplant, tomato, and pepper production. To improve the efficacy of bacterial wilt management, the goals of this study were to (1) conduct a survey of RSSC pathogens in Bangladesh and Nepal, (2) characterize the genetic diversity of these isolates, and (3) screen 37 tomato, eggplant, and pepper accessions for resistance to six representative isolates from South Asia. We isolated 99 isolates from Bangladesh and 20 isolates from Nepal and determined that all are phylotype I isolates of the Ralstonia pseudosolanacearum species. We sequenced and assembled draft genomes for 25 isolates. Phylogenomic analyses suggest that there is a wide diversity of endemic phylotype I isolates in South Asia, and possible introductions of two clonal phylotype I lineages into Bangladesh and Nepal. We contextualize our newly described isolates based on prior reports of RSSC diversity in South Asia and global reports of RSSC pathogens on eggplant and pepper. Greenhouse trials revealed multiple tomato, eggplant, and pepper accessions that exhibit promising levels of resistance to six phylotype I isolates from South Asia.\",\"PeriodicalId\":508090,\"journal\":{\"name\":\"PhytoFrontiers™\",\"volume\":\"27 4\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-12-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PhytoFrontiers™\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1094/phytofr-10-23-0136-r\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PhytoFrontiers™","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1094/phytofr-10-23-0136-r","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Characterization of Ralstonia pseudosolanacearum diversity and screening tomato, pepper, and eggplant resistance to manage bacterial wilt in South Asia
In South Asia, bacterial wilt pathogens in the Ralstonia solanacearum species complex (RSSC) impose major constraints on eggplant, tomato, and pepper production. To improve the efficacy of bacterial wilt management, the goals of this study were to (1) conduct a survey of RSSC pathogens in Bangladesh and Nepal, (2) characterize the genetic diversity of these isolates, and (3) screen 37 tomato, eggplant, and pepper accessions for resistance to six representative isolates from South Asia. We isolated 99 isolates from Bangladesh and 20 isolates from Nepal and determined that all are phylotype I isolates of the Ralstonia pseudosolanacearum species. We sequenced and assembled draft genomes for 25 isolates. Phylogenomic analyses suggest that there is a wide diversity of endemic phylotype I isolates in South Asia, and possible introductions of two clonal phylotype I lineages into Bangladesh and Nepal. We contextualize our newly described isolates based on prior reports of RSSC diversity in South Asia and global reports of RSSC pathogens on eggplant and pepper. Greenhouse trials revealed multiple tomato, eggplant, and pepper accessions that exhibit promising levels of resistance to six phylotype I isolates from South Asia.