Anatomical Record最新文献

{"title":"New evidence from Le Moustier 1: computer-assisted reconstruction and morphometry of the skull.","authors":"M S Ponce de León,&nbsp;C P Zollikofer","doi":"10.1002/(sici)1097-0185(19990401)254:4<474::aid-ar3>3.3.co;2-v","DOIUrl":"https://doi.org/10.1002/(sici)1097-0185(19990401)254:4<474::aid-ar3>3.3.co;2-v","url":null,"abstract":"<p><p>In this study, we present a new computerized reconstruction of the Le Moustier 1 Neanderthal skull and discuss its significance for Neanderthal growth and variability. Because of the precarious state of preservation of the original material, we applied entirely noninvasive methods of fossil reconstruction and morphometry, using a combination of computed tomography, computer graphics, and stereolithography. After electronic restoration, the isolated original pieces were recomposed on the computer screen using external and internal anatomical clues to position the bone fragments and mirror images to complete missing parts. The inferred effects of general compressive deformation that occurred during fossilization were corrected by virtual decompression of the skull. The resulting new reconstruction of the Le Moustier 1 skull shows morphologic features close to the typical Neanderthal adult state. Residual asymmetry of skeletal parts can be traced to in vivo skeletal modification: the left mandibular joint shows signs of a healed condylar fracture, and the anatomy of the occipital region suggests mild plagiocephaly. Using micro-CT analysis, the left incus could be recovered from the matrix filling of the middle ear cavity. Its morphometric dimensions are similar to those of the La Ferrassie III incus. The morphometric characteristics of the inner ear deviate substantially from the condition reported as typical for Neanderthals and fall within the range of modern human variability.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"254 4","pages":"474-89"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21074689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial cristae diversity in human Leydig cells: a revised look at cristae morphology in these steroid-producing cells.","authors":"F P Prince","doi":"10.1002/(SICI)1097-0185(19990401)254:4<534::AID-AR8>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(19990401)254:4<534::AID-AR8>3.0.CO;2-#","url":null,"abstract":"<p><p>Mitochondria of steroid-producing cells are integrally involved with steroidogenesis. For decades, the mitochondrial morphology of Leydig cells, as with other steroid-producing cells, has been known to differ from typical mitochondria in that the cristae are predominately \"tubular.\" In a few species, humans being one example, the cristae have often been further categorized as \"tubular and/or lamellar,\" without further elaboration. In the present study, mitochondria of human Leydig cells were examined with the purpose of providing a more detailed description of \"cristae\" morphology in these steroid-producing cells. The cristae are found to be rather diverse in morphology, consisting of elements of anastomosing tubules in continuity with small cisternal regions as well as with stacked arrays of lamellae, referred to as \"lamellar associations.\" The tubular elements are found to branch in a tripartite fashion and sometimes to expand into small cisternal elements at these junctures. The lamellar associations are a distinctive feature of cristae in human Leydig cells and consist of two to eight closely apposed lamellae with a consistent gap of approximately 4 nm between the membranes of apposing lamellae. Such a close association of cellular membranes is highly suggestive of an integral transmembrane linkage. Although the lamellar associations often appear isolated, evidence is present of a continuity of this compartment of the cristae with the tubular elements. The connections (termed \"initial segments\") of the various forms of the cristae to the inner mitochondrial membrane are typically via tubules. Mitochondria exhibiting a central region of matrix delineated by one or more cup-shaped lamellae are also present. The pleomorphic structure of mitochondrial cristae in human Leydig cells reemphasizes our present lack of knowledge of how subcellular structure relates to steroidogenesis.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"254 4","pages":"534-41"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21074532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degeneration of the seminiferous epithelium following epididymal obstruction in prepubertal rats.","authors":"C J Flickinger,&nbsp;M L Baran,&nbsp;S S Howards,&nbsp;J C Herr","doi":"10.1002/(SICI)1097-0185(19990101)254:1<76::AID-AR10>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(19990101)254:1<76::AID-AR10>3.0.CO;2-#","url":null,"abstract":"<p><p>The development of the testes was studied in rats following prepubertal obstruction of the epididymis. Male rats received bilateral ligation of the corpus epididymidis or a sham operation at 10 days of age, and temporal changes in testicular morphology and weights of reproductive organs were determined at intervals spanning sexual maturation. Development of the testes was normal through 35 days of age. The initial histological changes in the testes of ligated animals, observed at 56 days, included an increased diameter of the seminiferous tubule lumen, depletion of spermatids, and the presence of multinucleate spermatids. Subsequently, germ cells were greatly depleted in the testes of 91- and 128-day-old rats with ligated epididymides. After puberty, testicular weight and volume declined relative to corresponding sham-operated animals. On the other hand, the weights of the epididymides in ligated animals prior to puberty significantly exceeded those of sham-operated rats but weighed significantly less than those of rats in the sham group after sexual maturation. Testicular alterations occurred after increases in the weights of the epididymides. Testicular changes may have contributed to rather than resulted from an autoimmune response to spermatozoa because testicular alterations preceded increases in antisperm autoantibodies.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"254 1","pages":"76-86"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20798930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress fiber networks in sinus endothelial cells in the rat spleen.","authors":"K Uehara,&nbsp;M Miyoshi","doi":"10.1002/(SICI)1097-0185(19990101)254:1<22::AID-AR4>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(19990101)254:1<22::AID-AR4>3.0.CO;2-#","url":null,"abstract":"The wall of the splenic sinus is well known to be a critical site in the control of the blood‐cell passage through the splenic cord. However, there is little information on what mechanism controls the blood‐cell passage between the sinus endothelial cells adhered with intercellular junctions.","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"254 1","pages":"22-7"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20799019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Programmed cell death in the development of the mouse external auditory canal.","authors":"K Nishizaki,&nbsp;M Anniko,&nbsp;Y Orita,&nbsp;Y Masuda,&nbsp;T Yoshino,&nbsp;S Kanda,&nbsp;J Sasaki","doi":"10.1002/(SICI)1097-0185(199811)252:3<378::AID-AR5>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199811)252:3<378::AID-AR5>3.0.CO;2-#","url":null,"abstract":"<p><p>Programmed cell death (PCD) is an essential event for development. The purpose of this work was to ascertain how PCD, in vivo designated apoptosis, is involved in the development of the external auditory canal. We performed a time sequence study of the distribution of apoptosis during the development of external auditory canal (EAC) of the mouse. ICR mice ranging in age from embryonic day 11.5 (E11.5) to 12 days after birth (DAB) were used in the present study. A part of each head including both ears was removed and was processed according to its purpose. Light and electron microscopy for morphological studies and TUNEL method (Gavrieli et al. [1992] J Cell Biol., 119:493-501) for histochemical studies were used. On E11.5, distinct TUNEL-positive staining occurred in the branchial arch. Between E15.5 and 1DAB, TUNEL-positive cells were observed throughout the EAC and the number of these cells decreased with age. On E15.5 and E16.5, numerous TUNEL-positive cells were observed in a cavity remained in the epithelial plate. Transmission electron microscopy revealed that these cells had the features of apoptosis. From 3-12 DAB, no apoptosis was observed in the EAC except for the terminal differentiation of the skin of the EAC. Apoptosis was not observed during recanalization of the EAC, but occurred during the formation of the epithelial plate. The investigation established that PCD is involved in the formation of the epithelial plate, whereas only cornification of the epithelium of the EAC is associated with recanalization.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"252 3","pages":"378-82"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199811)252:3<378::AID-AR5>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20722335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Demonstration of extracellular acid phosphatase activity in the involuting, antimesometrial decidua in fed and acutely fasted mice by combined cytochemistry and electron microscopy.","authors":"S G Katz","doi":"10.1002/(SICI)1097-0185(199809)252:1<1::AID-AR1>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199809)252:1<1::AID-AR1>3.0.CO;2-#","url":null,"abstract":"<p><p>An ultrastructural cytochemical study of acid phosphatase activity in the antimesometrial decidua on days 9-11 of pregnancy was performed in fed and acutely fasted mice. Specimens were fixed in a buffered mixture of paraformaldehyde and glutaraldehyde and were incubated in a buffered medium containing sodium beta-glycerophosphate and cerium chloride for ultrastructural localization of acid phosphatase activity. Fed and fasted animals showed extracellular acid phosphatase reaction product in the decidual-trophoblast interface, in the region of loosely and tightly packed, mature decidual cells, and in the region of predecidual cells. Reaction product was absent in the region of nondecidualized stromal cells. Extracellular acid phosphatase activity was more conspicuous in the region of mature decidual cells in fasted mice than in fed mice, and it was apparently similar in the region of predecidual cells in both fed and fasted mice. Acid phosphatase reaction product was also observed in lysosomes in all cells studied. Because acid phosphatase activity reflects the presence of lysosomal hydrolases in general, our results suggest that there is matrix degradation by lysosomal enzymes in both fed and fasted mice. These events may be part of the process of tissue remodeling in regions of predecidual cells and mature decidual cells. However, it is also possible that, in the region of mature decidual cells, breakdown of matrix constituents is a mechanism to provide nutrients for the growing fetus. This mechanism is probably enhanced in fasted mice.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"252 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199809)252:1<1::AID-AR1>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20652979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1998 R.R. Bensley Award: Keith E. Mostov, University of California at San Francisco.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"253 4","pages":"101"},"PeriodicalIF":0.0,"publicationDate":"1998-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20653892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of colchicine on the enucleation of erythroid cells and macrophages in the liver of mouse embryos: ultrastructural and three-dimensional studies.","authors":"Y Sonoda,&nbsp;K Sasaki,&nbsp;M Suda,&nbsp;C Itano,&nbsp;H Iwatsuki","doi":"10.1002/(SICI)1097-0185(199807)251:3<290::AID-AR3>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199807)251:3<290::AID-AR3>3.0.CO;2-#","url":null,"abstract":"<p><p>Enucleation is the last event in the development of a definitive erythroid line, and extruded nuclei are phagocytosed by macrophages. Both colchicine and cytochalasin have been known to exert a great influence on the enucleation process, but the relationship between enucleation and these agents has not yet been clearly revealed in vivo. Our aim was to clarify the significance of the enucleation in liver erythropoiesis and macrophage phagocytosis by colchicine and cytochalasin administration to embryonic mice. Pregnant mice were intraperitoneally injected with colchicine or cytochalasin at 13 days of gestation. Embryonic livers were removed at intervals of 3, 6 and 12 h after injection for processing for light and electron microscopy, and, to obtain three-dimensional morphology of erythroids at enucleation, computer-aided reconstructions were performed by light microscopy. Colchicine injections had cytolytic effects on hepatocytes and macrophages, and numerous erythroblasts were observed in the process of enucleation after colchicine injection. However, the extruding nuclei were irregularly shaped, and some erythroblasts at mitosis showed extreme peripheralization of their chromosomal masses and cell membrane constriction. Enucleation behavior could also be observed in immature erythroblasts. Liver macrophages engulfed extruded nuclei and erythroblasts in mitosis. Cytochalasin injections, on the other hand, had no significant effect on embryonic livers. The progress of erythroblast mitosis was clearly stopped by colchicine injection, and numerous erythroblasts at mitosis were extruding their nuclear compartment. Following colchicine injection, erythroid enucleation also took place in immature erythroblasts, and mitotic erythroids were phagocytosed. In enucleation, more attention should be paid to hematopoietic environmental factors than to hemopoietic cell factors.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"251 3","pages":"290-6"},"PeriodicalIF":0.0,"publicationDate":"1998-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199807)251:3<290::AID-AR3>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20587004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical and morphometric studies of the fetal pancreas in diabetic pregnant rats. Effects of insulin administration.","authors":"R M Calvo,&nbsp;R Forcen,&nbsp;M J Obregon,&nbsp;F Escobar del Rey,&nbsp;G Morreale de Escobar,&nbsp;J Regadera","doi":"10.1002/(SICI)1097-0185(199806)251:2<173::AID-AR4>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199806)251:2<173::AID-AR4>3.0.CO;2-#","url":null,"abstract":"<p><strong>Background: </strong>Maternal diabetes influences fetal pancreas development. As there are some controversial reports, we studied the morphometric changes of the fetal insular pancreas and insulin immunostain of beta cells as well as the proliferative activity of insular cells in 21-day-old fetuses from control, diabetic, and insulin-treated diabetic pregnant rats.</p><p><strong>Methods: </strong>Streptozotocin was injected into 7-day-pregnant rats (controls were not injected). Some rats were either left untreated (diabetic) or injected with insulin. Animals were killed at 21 days of gestation. Fetal pancreas were fixed in toto for the morphometry and immunohistochemistry studies using anti-insulin, anti-Ki-67 and anti-proliferating cell nuclear antigen (PCNA) antibodies.</p><p><strong>Results: </strong>Diabetic status was determined by measuring maternal and fetal serum glucose and insulin levels. The morphometric studies showed hyperplasia of the diabetic fetal insular tissue which had not been normalized by insulin therapy. Diabetes caused an increase of both insulin-positive and insulin-negative cells. The increase in insulin-positive cells was not corrected by insulin treatment, although the number of non-beta cells became normal. The nuclear area in beta cells increased in diabetic rats but was not corrected by insulin. The cytoplasmic area decreased in diabetic rats and was normalized by insulin administration. Diabetes increased the expression of the nuclear antigen Ki-67 in fetal insular pancreas, and insulin treatment returned it to the normal state.</p><p><strong>Conclusions: </strong>Maternal diabetes leads to hyperstimulation of fetal beta cells, with increased proliferative activity. Insulin administration to the dams corrects some of the changes observed.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"251 2","pages":"173-80"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199806)251:2<173::AID-AR4>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20543484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fetal development of the pituitary gland in the beagle.","authors":"F Sasaki,&nbsp;S Nishioka","doi":"10.1002/(SICI)1097-0185(199806)251:2<143::AID-AR1>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199806)251:2<143::AID-AR1>3.0.CO;2-#","url":null,"abstract":"<p><strong>Background: </strong>The pituitary gland of the dog is different from all animals and is described as \"typical\" for mammals. How might this complex pituitary gland of the dog be formed in fetal life? The current study examined the fetal development of the complex and unique dog pituitary and the ontogeny of specific cell types in the pars distalis.</p><p><strong>Methods: </strong>Adenohypophysis of the beagle, from 25 to 60 days of gestation and at 2 days of age, was studied by immunocytochemical and histological staining.</p><p><strong>Results: </strong>At 25 days gestation, the primordium (Rathke's pouch) of the adenohypophysis began to form by an upward evagination from the epithelium of the primary oral cavity. At 38 days, the pituitary gland showed the same morphology as in adult dogs, being merely smaller. Five walls of Rathke's pouch (anterior wall [A], lateral walls [L], posterior wall [P], and upper wall [U] were found at 25 days, and by 38 days they had specialized into specific regions of the adenohypophysis through complex and unique processes. The pars intermedia was derived from the U and the dorsal area of the A. The pars tuberalis was derived from the dorsal area of the A. The pars distalis was formed by more singular processes: the peripheral areas of the pars distalis were first formed by A and P; then the ventral lumen of the extensive Rathke's lumen surrounded by these areas was filled up by proliferation of cells, although the dorsal lumen remains as Rathke's lumen after 38 days. The blood capillaries may play an important role in the development of parencymal cells in the Rathke's pouch during canine fetal life. At 30 days gestation, the first adrenocorticotropic hormone cells were found in the anterior- and posterior-ventral regions (derived from middle and ventral areas of the A and the P) of the pars distalis anlage, and blood capillaries invaded the parenchymal cells from the mesencyme surrounding the anlage. At 38 days, portal vessels without capillary loops in the median eminence had appeared, and growth hormone and luteinizing hormone cells appeared in the ventral areas of A and P in the pars distalis. By 52 days, when capillary loops were seen in the portal vessels in the median eminence, these types of cells spread through the whole pars distalis.</p><p><strong>Conclusion: </strong>These areas in the epithelium of Rathke's pouch at 25 days may differentiate into specific regions of the pars distalis during subsequent fetal life, through complex processes that are characteristics to the canine species.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"251 2","pages":"143-51"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199806)251:2<143::AID-AR1>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20544235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}