Horticulturae最新文献

{"title":"Combined Transcriptomic and Metabolomic Analyses Reveal the Mechanisms by Which the Interaction Between Sulfur and Nitrogen Affects Garlic Yield and Quality","authors":"Licai Ren, Xudong Pan, Yang Deng, Zheng Ge, Shiyuan Li, Dong Su, Guoqian Zhao, Hui Tang, Xiangfei Wang","doi":"10.3390/horticulturae10111203","DOIUrl":"https://doi.org/10.3390/horticulturae10111203","url":null,"abstract":"Nitrogen and sulfur are essential macronutrients in plant growth and development, and their interaction profoundly influences gene expression, metabolic activities, and adaptability in plants, directly affecting plant growth and yield. Garlic (Allium sativum L.) is a crop of significant economic and medicinal value. However, despite the critical role of the nitrogen–sulfur interaction in garlic’s adaptability, yield, and quality, the specific mechanisms underlying these effects remain unclear. In this study, transcriptomic and metabolomic analyses were employed to investigate the effects of combined sulfur and nitrogen application on garlic bulb tissues. The results show that the combined application of sulfur and nitrogen significantly increased the diameter and weight of garlic bulbs by 14.96% and 35.47%, respectively. The content of alliin increased by 28.48%, while the levels of abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), and gibberellin (GA) increased by 15.82%, 12.94%, 32.34%, and 48.13%, respectively. Additionally, the activities of alliinase, superoxide dismutase (SOD), and catalase (CAT) were enhanced by 7.93%, 4.48%, and 19.74%, respectively. Moreover, the application of sulfur and nitrogen significantly reduced the malondialdehyde (MDA) content and peroxidase (POD) activity in garlic bulbs by 29.66% and 9.42%, respectively, thereby improving garlic’s adaptability and growth potential. Transcriptomic analysis revealed differentially expressed genes in several key pathways, including plant hormone signal transduction, RNA degradation, glutathione metabolism, amino acid biosynthesis, and glycerophospholipid metabolism. Metabolomic analysis identified 80 differentially abundant metabolites primarily consisting of amino acids, indole carboxylic acids, and fatty acids. The integrated transcriptomic and metabolomic analyses highlighted the pivotal roles of glutathione metabolism, glycerophospholipid metabolism, and amino acid biosynthesis pathways in the synergistic effects of sulfur and nitrogen. This study not only provides critical scientific evidence for understanding the mechanisms underlying the nitrogen–sulfur interaction’s impact on the yield and quality of garlic but also offers a scientific basis for optimizing nutrient management strategies to enhance garlic yield and quality.","PeriodicalId":507445,"journal":{"name":"Horticulturae","volume":"10 11","pages":"1203-1203"},"PeriodicalIF":0.0,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2311-7524/10/11/1203/pdf?version=1731674220","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and Physiological Analyses of Resistant and Susceptible Pepper (Capsicum annuum) to Verticillium dahliae Inoculum","authors":"Xinmin Huang, Li-Ming He, Huimin Tan, Jiayi Liu, Qiucheng Qiu, Qidi Sun, Lejun Ouyang, Hanbing Han, Qinqin He","doi":"10.3390/horticulturae10111160","DOIUrl":"https://doi.org/10.3390/horticulturae10111160","url":null,"abstract":"Pepper (Capsicum annuum) is a globally important vegetable, and Verticillium wilt is an important disease affecting peppers and is caused by Verticillium dahliae, which can severely reduce yields. However, the molecular mechanisms underlying the responses of pepper to infection by V. dahliae are largely unknown. We performed physiological and transcriptome analysis using resistant and susceptible pepper cultivars inoculated with V. dahliae. Compared to the susceptible cultivar MS66, the resistant cultivar MS72 retained higher chlorophyll content and lower malondialdehyde content after inoculation. At 3 days after inoculation (DAI), compared with MS66, 534 differentially expressed genes (DEGs) were identified in MS72. At 5 DAI, 2392 DEGs were identified in MS72 compared with MS66. The DEGs in MS72 were mainly enriched in the cell wall and photosynthesis-related Gene Ontology terms, as well as in pathways such as cutin, suberin, wax biosynthesis, phenylpropanoid biosynthesis, and photosynthesis. Using weighted gene co-expression network analysis, 36 hub genes involved in the resistance response were identified, including the transcription factor bHLH93 (Capana04g000815), defense-like protein 1 (MSTRG.5904), and miraculin-like (Capana10g002167). Our findings contribute to a more comprehensive understanding of the response mechanism of pepper to V. dahliae inoculation, providing new avenues for improving pepper resistance through breeding programs.","PeriodicalId":507445,"journal":{"name":"Horticulturae","volume":"10 11","pages":"1160-1160"},"PeriodicalIF":0.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147880433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and Characterization of Lasiodiplodia pseudotheobromae Associated with Stem Wilt on Ficus hirta (Vahl) and Its Fungicidal Sensitivity","authors":"Chunping He, He Wu, Yue Hu, Rui Li, Jinjing Lin, Ying Lü, Zi-wei Gu, Shibei Tan, Yanqiong Liang","doi":"10.3390/horticulturae10101069","DOIUrl":"https://doi.org/10.3390/horticulturae10101069","url":null,"abstract":"Ficus hirta Vahl is an important medicinal and edible plant in southern China. Typical green wilting on leaves and brown necrotic spots on the stems were observed since mid-June 2022 in an F. hirta plantation in Danzhou, Hainan (China). The disease rapidly developed, causing stem withering and plant death. The disease incidence varied from 45 to 85% prevalence, and the average disease index was 47 in the period of outbreak during June to December. Relevant hypothetical fungi were isolated from naturally infected wilt tissues, and their pathogens were preliminarily confirmed to be Lasiodiplodia pseudotheobromae through hypothetical fungal culturing, morphological characteristic observations, and pathogenicity testing on F. hirta plants. The phylogenetic tree constructed based on partial ITS, translation elongation factor (TEF1-α), and the β-tubulin gene (TUB2) further confirmed the identity of the pathogen as L. pseudotheobromae. Further research on the biological characteristics of L. pseudotheobromae showed that the optimal temperature for the growth of L. pseudotheobromae was PDA medium, with a temperature of 30 °C and pH of 6. Peptone and fructose were the optimal nitrogen and carbon sources for it. In vitro efficacy testing showed that among eleven fungicides, fluazinam and prochloraz had the highest mycelial growth inhibition, with an EC50 of 0.0477 µg/mL and 0.0996 µg/mL, respectively. And the two fungicides showed significant control on the stem wilt of F. hirta in a pot. To our knowledge, this is the first comprehensive report on the pathogen identification and biological characteristics of L. pseudotheobromae infecting the stem wilt of F. hirta in China. Our results provide important information for developing effective management measures and controlling this disease.","PeriodicalId":507445,"journal":{"name":"Horticulturae","volume":"10 10","pages":"1069-1069"},"PeriodicalIF":0.0,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of BraSWEET12 in Regulating Flowering through Sucrose Transport in Flowering Chinese Cabbage","authors":"Qinqin He, Li-Ming He, Zongqin Feng, Yin Liu, Yunyi Xiao, Jinfeng Liu, Hanbing Han, Xinmin Huang","doi":"10.3390/horticulturae10101037","DOIUrl":"https://doi.org/10.3390/horticulturae10101037","url":null,"abstract":"We assessed the flowering Chinese cabbage (Brassica rapa var. parachinensis), a specialty vegetable found in southern China. The sugar content of the stem tip is closely related to bolting and flowering. Sugar Will Eventually be Exported Transporters (SWEETs) are bidirectional sugar transporter proteins involved in numerous plant growth and development processes. The expression of BraSWEET12 is positively correlated with sugar content. However, it is unclear whether BraSWEET12 is involved in bolting and flowering. In this study, we identified and characterized BraSWEET12. BraSWEET12 in flowering Chinese cabbage contains 288 amino acids and is located on the cell membrane as a sucrose transporter protein. BraSWEET12 is highly expressed in the petals and stem tips of flowering Chinese cabbage and is upregulated by gibberellin and low temperatures. Overexpression of BraSWEET12 in Arabidopsis can increase sucrose content at the stem tip, upregulate the expression of AtAP1 and AtLFY, and advance the flowering time. Subsequently, our results indicate that BraSWEET12 is involved in sucrose accumulation at the stem tip of flowering Chinese cabbage and plays a crucial role in flowering regulation. These results provide a reference for elucidating the regulatory mechanisms underlying flowering Chinese cabbage bolting and flowering.","PeriodicalId":507445,"journal":{"name":"Horticulturae","volume":"10 10","pages":"1037-1037"},"PeriodicalIF":0.0,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Sampling Strategies and Genetic Diversity Analysis of Red Beet Germplasm Resources Using SSR Markers","authors":"Xiangjia Wu, Zhi Pi, Shengnan Li, Zedong Wu","doi":"10.3390/horticulturae10091008","DOIUrl":"https://doi.org/10.3390/horticulturae10091008","url":null,"abstract":"By using 14 SSR primer pairs, we here analyzed and compared the amplification results of 534 DNA samples from six red sugar beet germplasm resources under three treatments. These data were used to explore the sampling strategy for the aforementioned resources. Based on the sampling strategy results, 21 SSR primer pairs were used to evaluate the genetic diversity of 47 red sugar beet germplasm resources. The six population genetic parameters used for exploring the sampling strategy unveiled that individual plants within the population had a relatively large genetic distance. The genetic parameters Ne, I, and Nei’s of the randomly mixed sampling samples increased rapidly between 10 and 30 plants before decreasing. Therefore, when SSR technology was used to analyze the genetic diversity of the red sugar beet germplasm resources, the optimal sampling gradient for each population was the adoption of a random single-plant mixed sampling sample of no less than 10 plants and no more than 30 plants. The 21 SSR primer pairs were used to detect genetic diversity in 30 random mixed samples of 47 resources. The average polymorphic information content (PIC) was 0.5738, the average number of observed alleles (Na) was 4.1905, the average number of effective alleles (Ne) was 2.8962, the average Shannon’s information index (I) was 1.1299, the average expected heterozygosity (Nei’s) was 0.6127, and the average expected heterozygosity (He) was 0.6127. The genetic distance of the 47 germplasm resources ranged from 0.0225 to 0.551 (average: 0.316). According to the population structure analysis, the most suitable K value was six, which indicated the presence of six populations. Based on the clustering analysis results, the 47 germplasm resources were segregated into six groups, with obvious clustering and some germplasm resources noted for having groups with close genetic relationships. We here established a more accurate and scientific sampling strategy for analyzing the genetic diversity of red sugar beet germplasm resources by using SSR molecular markers. The findings provide a reference for collecting and preserving red sugar beet germplasms and protecting their genetic diversity.","PeriodicalId":507445,"journal":{"name":"Horticulturae","volume":"10 9","pages":"1008-1008"},"PeriodicalIF":0.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2311-7524/10/9/1008/pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amino Acid Transporters on Amino Acid Absorption, Transport and Distribution in Crops","authors":"Lu Liu, Xianchang Yu, Yan Yan, Chaoxing He, Jun Wang, Mintao Sun, Yansu Li","doi":"10.3390/horticulturae10090999","DOIUrl":"https://doi.org/10.3390/horticulturae10090999","url":null,"abstract":"Amino acid transporters mediate amino acid transport within and between cells and are the main mediators of nitrogen distribution in plants, which is crucial for maintaining plant growth and development. Many amino acid transporters have been identified in different plant species. In this review, we discuss the functions of amino acid transporters in plant absorption and amino acid transportation from root to shoot, which results in crop yield and quality improvement. Moreover, we summarize the role of amino acid transporters in response to environmental stimuli and the influence of N and C metabolic processes. We also elaborate on potential future research directions.","PeriodicalId":507445,"journal":{"name":"Horticulturae","volume":"10 9","pages":"999-999"},"PeriodicalIF":0.0,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LvbHLH13 Regulates Anthocyanin Biosynthesis by Activating the LvMYB5 Promoter in Lily (Lilium ‘Viviana’)","authors":"Wenzhong An, Yibo Sun, Zhenhua Gao, Xiaoye Liu, Qi Guo, Shaokun Sun, Minghui Zhang, Yutong Han, Muhammad Irfan, Lijing Chen, Di Ma","doi":"10.3390/horticulturae10090926","DOIUrl":"https://doi.org/10.3390/horticulturae10090926","url":null,"abstract":"Anthocyanins, constituents of flavonoid compounds prevalent in plants, possess significant value in both plant development and human nutrition. The regulation of anthocyanin biosynthesis primarily involves the orchestration of MYB, bHLH, and WD40 transcription factors. Consequently, the bHLH family assumes a pivotal role in modulating plant developmental processes. In the present investigation, a transcription factor, denoted as LvbHLH13, was identified as a positive regulator of anthocyanin pigmentation in lily petals. LvbHLH13 is classified within the IIId subgroup of Arabidopsis bHLH proteins. Functional analyses involving the transient expression and gene silencing of LvbHLH13 revealed its capacity to enhance and diminish anthocyanin accumulation, respectively, by modulating the LvMYB5 expression, thereby influencing the downstream structural gene expression. The overexpression of LvbHLH13 resulted in an increase in the expression of the downstream structural genes related to anthocyanin synthesis, whereas silencing of LvbHLH13 correspondingly decreased the expression. Yeast one-hybrid and EMSA assays demonstrated the interaction between LvbHLH13 and the LvMYB5 promoter, leading to the activation of anthocyanin biosynthesis. A further luciferase (LUC) analysis corroborated the stimulatory effect of LvbHLH13 on the LvMYB5 promoter sequence. Consequently, LvbHLH13 assumed a crucial role in lily-petal pigmentation. A yeast two-hybrid analysis revealed that LvbHLH13 diverged from typical bHLH transcription factor behavior as it did not form a complex with MYB to regulate anthocyanin biosynthesis. This discrepancy could be attributed to the deletion of the N-terminal conserved sequence of LvbHLH13. This study provides a new bHLH candidate and bHLH-MYB partner to explore the anthocyanin regulatory network in further research and provides new opportunities for breeding lilies with various anthocyanin contents. These findings lay a theoretical foundation for subsequent investigations into lily flower coloring mechanisms.","PeriodicalId":507445,"journal":{"name":"Horticulturae","volume":"10 9","pages":"926-926"},"PeriodicalIF":0.0,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2311-7524/10/9/926/pdf?version=1724999218","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147913361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in Spectral Reflectance, Photosynthetic Performance, Chlorophyll Fluorescence, and Growth of Mini Green Romaine Lettuce According to Various Light Qualities in Indoor Cultivation","authors":"Joo Hwan Lee, Yong Beom Kwon, In-Lee Choi, Hyuk Sung Yoon, Jidong Kim, Yong-Duk Kim, Ho-Min Kang","doi":"10.3390/horticulturae10080860","DOIUrl":"https://doi.org/10.3390/horticulturae10080860","url":null,"abstract":"Light quality can be stated to be the identity of an artificial light source, and although the response of light quality may vary depending on the crop, the effect is clearly visible and can produce various results depending on the combination of an artificial light source. This study investigated the spectral reflectance, photosynthetic performance, and chlorophyll fluorescence of mini green romaine lettuce based on light quality. Most parameters related to spectral reflectance showed the best results under blue light, and photosynthetic performance was more effective with mixed light than with single-colored light, among which blue + red (BR)-LED was the most suitable. Red light was ineffective, showing mostly low results in parameters of spectral reflectance and photosynthetic performance. In the case of chlorophyll fluorescence, the light quality influenced photomorphogenesis, resulting in increased leaf length and width with R- and quantum dot (QD)-LED, which expanded the leaf area and allowed for more external light to be captured (ABS/RC and TRo/RC). However, the ratio of electronized energy (ETo/RC) was low, and the amount of energy dissipated as heat (DIo/RC) was high. Consequently, the degree of electron acceptor reduction and overall photosynthetic performance (PIABS and PItotal) were lower compared to other light qualities. Additionally, the contrasting results of QD-LED and BR-LED were attributed to the form of red light and the presence or absence of far-red light when comparing spectra. Principal component analysis also clearly distinguished light qualities for photosynthesis and growth. Growth was increased by red (R)- and QD-LED, while photosynthetic performance was increased by BR- and blue (B)-LED.","PeriodicalId":507445,"journal":{"name":"Horticulturae","volume":"10 8","pages":"860-860"},"PeriodicalIF":0.0,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147917429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Yadav et al. Assessment of Gene Action and Identification of Heterotic Hybrids for Enhancing Yield in Field Pea. Horticulturae 2023, 9, 997","authors":"Indu Yadav, Vijay Sharma, Mukul Kumar, L. P. Yadav, Anuj Mishra, Vivek Singh, Parmdeep Singh Dhanda, Alpa Yadav, Manisha Yadav, Shalesh Kumar Singh, Kamaluddin, P. Prajapati, Prashant Kaushik, Vaishali Gangwar","doi":"10.3390/horticulturae10070749","DOIUrl":"https://doi.org/10.3390/horticulturae10070749","url":null,"abstract":"The Horticulturae Editorial Office wishes to make the following changes to the author’s paper [...]","PeriodicalId":507445,"journal":{"name":"Horticulturae","volume":"7 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141642786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Soil Management Practices on Soil Culturable Bacteriota and Species Diversity in Central European a Productive Vineyard under Warm and Dry Conditions","authors":"V. Šimanský, M. Kačániová, Martin Juriga, Natália Čmiková, Petra Borotová, Elena Aydın, E. Wójcik-Gront","doi":"10.3390/horticulturae10070753","DOIUrl":"https://doi.org/10.3390/horticulturae10070753","url":null,"abstract":"Sustainable management practices are crucial for the longevity of a monoculture vineyard, especially in the context of a changing climate. Therefore, soil management practices in a vineyard (T: tillage, T+FYM: tillage + farmyard manure, G: grass strips, G+NPK1: grass strips + rational rates of NPK, and G+NPK2: grass strips + higher rates of NPK) were tested in a temperate climate of Slovakia (Central Europe) under specific soil conditions (Rendzic Leptosol). We investigated the influence of continuous cropping on soil chemical properties and microbial communities during the dry and warm year of 2022. The results showed that the soil pH was higher by 19%, 21%, 24% and 13% in T, T+FYM, G and G+NPK1, respectively, compared to G+NPK2. The lowest soil organic matter (SOM) content was found in T, and it increased in the following order: T < T+FYM < G+NPK2 < G+NPK1 < G. Similarly, the lowest abundance of soil culturable bacteriota was found in T and it increased in the following order: T < T+FYM = G+NPK2 < G+NPK1< G. Culturable bacteriota was identified using mass spectrometry (MALDI-TOF MS Biotyper). The most numerous species group was Bacillus, followed by Lactobacillus > Staphylococcus > Pseudomonas. The most frequently isolated species were Bacillus megaterium (16.55%), Bacillus cereus (5.80%), Bacillus thuringiensis (4.87%), and Bacillus simplex (4.37%). Positive relationships between SOM and soil culturable bacteriota were found in the G and G+NPK1 treatments. Temperature also affected soil culturable bacteriota in all soil management practices, most significantly in G+NPK1. Overall, the best scenario for the sustainable management of a productive vineyard is the use of grass strips.","PeriodicalId":507445,"journal":{"name":"Horticulturae","volume":"10 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141640560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}