Biochimica et Biophysica Acta-Bioenergetics最新文献

{"title":"Mitochondrial reactive oxygen species production in lungs of rats with different susceptibilities to hyperoxia-induced acute lung injury","authors":"Pardis Taheri ,&nbsp;Devanshi D. Dave ,&nbsp;Abraham Taye ,&nbsp;Anne V. Clough ,&nbsp;Elizabeth R. Jacobs ,&nbsp;Ranjan K. Dash ,&nbsp;Said H. Audi","doi":"10.1016/j.bbabio.2025.149561","DOIUrl":"10.1016/j.bbabio.2025.149561","url":null,"abstract":"<div><div>Adult rats exposed to hyperoxia (&gt;95 % O₂) die within 60–72 h from respiratory failure. However, when preconditioned with either &gt;95 % O₂ for 48 h followed by 24 h in room air (H-T) or 60 % O₂ for 7 days (H-S), they acquire tolerance or susceptibility to hyperoxia, respectively. The aim was to quantify H₂O₂ production rate and identify sources in isolated lung mitochondria and isolated perfused lungs (IPLs) of normoxia, H-T, and H-S rats. Mitochondria were isolated from lungs, and H₂O₂ production rates were quantified in the presence of pyruvate-malate or succinate, with and without inhibitors of mitochondrial complex I (CI), complex II (CII), and/or H₂O₂ scavenging systems. Lung rate of H₂O₂ release was quantified in IPLs with and without CII inhibitor. Results from isolated mitochondria show that CII is the main H₂O₂ source, and that both H₂O₂ production rate and scavenging capacity were ~48 % lower in H-S mitochondria compared to normoxia. Results from IPLs show that CII is also the dominant H₂O₂ source from lung tissue, and that H₂O₂ release rate was lower in H-T lungs compared to normoxia and H-S lungs. These results suggest that for H-S rats, both mitochondrial rate of H₂O₂ production and scavenging capacity were significantly lower than those in normoxia mitochondria and may contribute to their increased hyperoxia susceptibility. The lower H₂O₂ release rate from H-T IPLs, along with no change in mitochondrial H₂O₂ production rate, is consistent with higher antioxidant capacity in the lungs of H-T rats, which may contribute to their hyperoxia tolerance.</div></div>","PeriodicalId":50731,"journal":{"name":"Biochimica et Biophysica Acta-Bioenergetics","volume":"1866 4","pages":"Article 149561"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chamber oxygen concentration impacts mitochondrial function and hydrogen peroxide appearance in permeabilized human skeletal muscle fibers","authors":"Bradley A. Ruple ,&nbsp;Soung Hun Park ,&nbsp;Jesse C. Craig ,&nbsp;Matthew T. Lewis ,&nbsp;Joel D. Trinity ,&nbsp;Russell S. Richardson ,&nbsp;Ryan M. Broxterman","doi":"10.1016/j.bbabio.2025.149568","DOIUrl":"10.1016/j.bbabio.2025.149568","url":null,"abstract":"<div><div>Skeletal muscle mitochondrial respiration is commonly assessed ex vivo using permeabilized fibers in media with high oxygen (O₂) concentrations to ensure that O₂ availability does not limit respiration. However, high O₂ concentrations also increase the production of reactive O₂ species that can negatively affect respiration. In this study, we tested the hypotheses that permeabilized fiber mitochondria in a high, compared to low, O₂ concentration would (i) not be different at maximal state 3 respiration rate (V_max), (ii) have lower submaximal respiration rates at submaximal O₂ concentrations, and (iii) have greater total cumulative hydrogen peroxide (H₂O₂) appearance. We continuously monitored mitochondrial state 3 respiration and H₂O₂ appearance rates using high-resolution respirometry in permeabilized skeletal muscle fibers (12 untrained participants; 22 ± 4 yrs) with either control (~127 mmHg; CON) or high (~327 mmHg; HIGH) partial pressures of O₂ (PO₂). V_max was not different between conditions (HIGH: 80.7 ± 16.7 vs. CON: 82.3 ± 18.7 pmol/s/mg, <em>p</em> = 0.695). The PO₂ at 80 % V_max (P₈₀) was greater in HIGH (73.9 ± 25.5 vs. 28.0 ± 7.1 mmHg, <em>p</em> &lt; 0.001) and respiration rates at 5–60 mmHg PO₂ were lower for HIGH than CON (all <em>p</em> &lt; 0.001). Additionally, the total cumulative H₂O₂ appearance was greater in HIGH than CON (<em>n</em> = 11; 51.5 ± 23.2 vs. 18.3 ± 10.3 pmol/mg, <em>p</em> &lt; 0.001), and this difference was directly correlated with the difference in P₈₀ (<em>r</em> = 0.655, <em>p</em> = 0.029). The current findings support that a high O₂ concentration, by itself, does not appear to affect V_max in the permeabilized skeletal muscle fiber preparation, but the corollary increase in H₂O₂ exposure may diminish mitochondrial state 3 respiratory function.</div></div>","PeriodicalId":50731,"journal":{"name":"Biochimica et Biophysica Acta-Bioenergetics","volume":"1866 4","pages":"Article 149568"},"PeriodicalIF":2.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144842126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conformational dynamics of a histidine molecular switch in a cation/proton antiporter","authors":"Cristina Pecorilla ,&nbsp;Anton Altmeyer ,&nbsp;Outi Haapanen ,&nbsp;Yongchan Lee ,&nbsp;Volker Zickermann ,&nbsp;Vivek Sharma","doi":"10.1016/j.bbabio.2025.149563","DOIUrl":"10.1016/j.bbabio.2025.149563","url":null,"abstract":"<div><div>Multisubunit Mrp (<u>m</u>ultiple <u>r</u>esistance and <u>p</u>H adaptation) type sodium proton antiporters are indispensable for the growth of alkali and salt tolerant bacteria and archaea. They share sequence and structural similarity with the membrane domain of respiratory complex I, a key mitochondrial enzyme. The molecular mechanism of complex I and Mrp antiporters has remained largely unknown and is the subject of intense debate. Here, by combining site-directed mutagenesis with large-scale molecular dynamics simulations, we explore the conformational dynamics of a key histidine residue in the MrpA subunit of the antiporter. We show that point mutations perturbing the conformational mobility of the histidine sidechain directly affect the transport activity of the antiporter. We identify that protonation state variations in conserved lysine residues around the histidine drive hydrogen bonding rearrangements and hydration changes coupled to sidechain and backbone conformational dynamics. Finally, we develop detailed and testable mechanistic models of proton transfer in Mrp antiporter and complex I, in which the histidine switch functions as a unique gating element.</div></div>","PeriodicalId":50731,"journal":{"name":"Biochimica et Biophysica Acta-Bioenergetics","volume":"1866 4","pages":"Article 149563"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deletion of AC8 in glioma cells elevates oxidative phosphorylation by system-wide remodeling of the mitochondrial proteome","authors":"Emil Jakobsen ,&nbsp;Jacob M. Bech ,&nbsp;Jens V. Andersen ,&nbsp;Emil W. Westi ,&nbsp;Martin R. Larsen ,&nbsp;Niels H. Skotte ,&nbsp;José M.A. Moreira ,&nbsp;Blanca I. Aldana ,&nbsp;Lasse K. Bak","doi":"10.1016/j.bbabio.2025.149567","DOIUrl":"10.1016/j.bbabio.2025.149567","url":null,"abstract":"<div><div>The Warburg effect is the reprogramming of cancer cells towards glycolytic metabolism, likely producing and releasing lactate into the tumor microenvironment. This lactate has been suggested to partly drive tumor growth by signaling through the lactate receptor, GPR81. Thus, reprogramming cancer cells away from glycolytic activity may be beneficial for cancer treatment. Here, we show that deletion of <em>ADCY8</em> (coding for adenylyl cyclase 8; AC8) employing the CRISPR-Cas9 technology in U87MG glioma cells, changes the proteome of these cells through a system-wide transformation in expression of mitochondrial proteins. These changes shift the metabolic balance towards oxidative phosphorylation, as shown by an increase in oxygen consumption, an elevation in tricarboxylic acid cycle flux, and a concomitant decrease in glycolytic flux. This metabolic shift is likely driven by the absence of AC8-mediated transcriptional regulation and may suggest that inhibition of AC8 activity could hold therapeutic potential in the treatment of cancer.</div></div>","PeriodicalId":50731,"journal":{"name":"Biochimica et Biophysica Acta-Bioenergetics","volume":"1866 4","pages":"Article 149567"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ATP requirements for growth reveal the bioenergetic impact of mitochondrial symbiosis","authors":"William F. Martin","doi":"10.1016/j.bbabio.2025.149564","DOIUrl":"10.1016/j.bbabio.2025.149564","url":null,"abstract":"<div><div>Studies by microbiologists in the 1970s provided robust estimates for the energy supply and demand of a prokaryotic cell. The amount of ATP needed to support growth was calculated from the chemical composition of the cell and known enzymatic pathways that synthesize its constituents from known substrates in culture. Starting in 2015, geneticists and evolutionary biologists began investigating the bioenergetic role of mitochondria at eukaryote origin and energy in metazoan evolution using their own, widely trusted—but hitherto unvetted—model for the costs of growth in terms of ATP per cell. The more recent model contains, however, a severe and previously unrecognized error that systematically overestimates the ATP cost of amino acid synthesis up to 200-fold. The error applies to all organisms studied by such models and leads to conspicuously false inferences, for example that the synthesis of an average amino acid in humans requires 30 ATP, which no biochemistry textbook will confirm. Their ATP ‘cost’ calculations would require that <em>E. coli</em> obtains ~100 ATP per glucose and that mammals obtain ~240 ATP per glucose, untenable propositions that invalidate and void all evolutionary inferences so based. By contrast, established methods for estimating the ATP cost of microbial growth show that the first mitochondrial endosymbionts could have easily doubled the host's available ATP pool, provided (i) that genes for growth on environmental amino acids were transferred from the mitochondrial symbiont to the archaeal host, and (ii) that the host for mitochondrial origin was an autotroph using the acetyl-CoA pathway. Stated in simple terms, the significance of these findings are this: Life is a chemical reaction. It requires energy release in order to proceed. The currency of energy in cells is adenosine triphosphate, ATP. Five decades ago, microbiologists were able to measure and understand the amount of ATP that cells require to grow. New studies by evolutionary biologists have appeared in the meantime that brush aside the older microbiological findings, using their own methods to calculate the ATP cost of growth instead. Science is, however, an imperfect undertaking. The new studies contain a major error, similar to conflating centimeters with yards. The error affects many publications and their conclusions. Using the old methods, we can still meaningfully study the role of energy in evolution, including the origin of complex, nucleus-bearing cells.</div></div>","PeriodicalId":50731,"journal":{"name":"Biochimica et Biophysica Acta-Bioenergetics","volume":"1866 4","pages":"Article 149564"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential effects of the D1/S264V mutation in photosystem II with either PsbA1 or PsbA3 on QB, non-heme Iron, and the associated hydrogen-bond network","authors":"Kosuke Tada ,&nbsp;Kaho Yamagata ,&nbsp;Kazumi Koyama ,&nbsp;Julien Sellés ,&nbsp;Alain Boussac ,&nbsp;Miwa Sugiura","doi":"10.1016/j.bbabio.2025.149557","DOIUrl":"10.1016/j.bbabio.2025.149557","url":null,"abstract":"<div><div>The role of the D1/S264 residue and the role of its environment in the proton-coupled electron transfer reaction on the acceptor side of Photosystem II were investigated. To this end, D1/S264V mutants were constructed in the thermophilic cyanobacterium <em>Thermosynechococcus elongatus</em>, with D1 being either PsbA1 or PsbA3. The PSII mutants were investigated using EPR spectroscopy, thermoluminescence, (time-resolved) absorption changes measurements, and oximetry. While the mutation had minor effects in PsbA1-PSII, the S264V mutation in PsbA3-PSII had significant consequences: <em>i</em>) thermoluminescence data show inefficient electron transfer from Q_A⁻ to Q_B; <em>ii</em>) re-oxidation of Q_A⁻ was slowed, by at least a factor of 10; <em>iii</em>) the herbicides inhibit weakly O₂ evolution; <em>iv</em>) no Fe²⁺Q_B⁻ EPR signal was detected in dark-adapted PSII; instead, <em>v</em>) a large Fe³⁺ signal was present with <em>vi</em>) modified EPR properties; <em>vii</em>) no Q_A⁻Fe²⁺Q_B⁻ biradical signal was observed after illumination at 198 K following a flash illumination, confirming the inefficient formation of Q_B⁻; <em>viii</em>) either no proton uptake coupled to non-heme iron reduction occurred or with a very slow rate compared to PsbA3-PSII; <em>ix</em>) changes were noted in the electrochromic response associated with Q_A⁻ formation; and <em>x</em>) increased production of singlet oxygen, both with and without herbicides. The S264V mutation in PsbA3-PSII leads to a significant decrease in the energy gap between the Q_A⁻Q_B and Q_AQ_B⁻ states. The effects listed above are discussed regarding the differences between PsbA1-PSII and PsbA3-PSII as those related to the sulfoquinovosyldiacylglycerol, the water molecules and the H-bond network.</div></div>","PeriodicalId":50731,"journal":{"name":"Biochimica et Biophysica Acta-Bioenergetics","volume":"1866 3","pages":"Article 149557"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three's company: Membrane waltz among organelles","authors":"Valentin Guyard,&nbsp;Francesca Giordano","doi":"10.1016/j.bbabio.2025.149555","DOIUrl":"10.1016/j.bbabio.2025.149555","url":null,"abstract":"<div><div>The study of membrane contact sites (MCS) has profoundly transformed our understanding of inter-organelle communication. These sites, where the membranes of two organelles are closely apposed, facilitate the transfer of small molecules such as lipids and ions. They are especially crucial for the maintenance of the structure and function of organelles like mitochondria and lipid droplets, which are largely excluded from vesicular trafficking. The significant advancements in imaging techniques, and molecular and cell biology research have shown that MCS are more complex than what originally thought and can involve more than two organelles. This has revealed the intricate nature and critical importance of these subcellular connections.</div><div>Here, we provide an overview of newly described three-way inter-organelles associations, and the proteins involved in these MCS. We highlight the roles these contacts play in key cellular processes such as lipid droplet biogenesis and mitochondrial division. Additionally, we discuss the latest advances in super-resolution imaging that enable the study of these complex three-way interactions. Ongoing research, driven by technological innovations, promises to uncover further insights into their roles in fundamental cellular processes and their implications for health and disease.</div></div>","PeriodicalId":50731,"journal":{"name":"Biochimica et Biophysica Acta-Bioenergetics","volume":"1866 3","pages":"Article 149555"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenine nucleotide-dependent Ca2+ buffering by mitochondria in an inorganic phosphate-free medium","authors":"Anna.B. Nikiforova,&nbsp;Maxim.V. Molchanov,&nbsp;Alexey G. Kruglov","doi":"10.1016/j.bbabio.2025.149559","DOIUrl":"10.1016/j.bbabio.2025.149559","url":null,"abstract":"<div><div>Inorganic phosphate (P_i) is essential for Ca²⁺ buffering by mitochondria. Adenine nucleotides (AN) are known to strongly increase the Ca²⁺-retention capacity (CRC) of mitochondria even in the absence of P_i in the medium. Several mechanisms can explain this phenomenon. Here we examined these mechanisms in detail in isolated rat liver mitochondria. We found that, in P_i-free medium, AN dose-dependently increased the CRC. The F_OF₁-ATP synthase (F-ATPase) inhibitor oligomycin decreased the CRC and the Ca²⁺ uptake rate to a minor extent. Nuclear magnetic resonance (NMR) analysis showed that P_i in suspensions of oligomycin-treated mitochondria was formed due to AN hydrolysis. In the absence and presence of Ca²⁺, mitochondria accumulated small and large (50 and &gt; 1000 nmol/mg protein) amounts of Pi, respectively, without detectable accumulation of AN. The average ratio of Ca²⁺ to P_i accumulated by intact mitochondria in the presence of ADP, ATP, and ATP plus P_i was about 0.68, 1, and 1.25, respectively, or lower. These values correspond to the formation of calcium dihydrogen and hydrogen orthophosphates, and tricalcium phosphate/whitlockite in different proportions. AN increased the CRC in the presence of inhibitors of both F-ATPase and adenylate translocase, the known regulators of the permeability transition pore (PTP). The PTP inhibitor NADH did not increase the CRC in the absence of P_i. Thus, the mechanism of the AN-dependent increase in the CRC in the absence of P_i includes the F-ATPase-independent production of P_i and suppression of the PTP at the site other than F-ATPase and adenylate translocase.</div></div>","PeriodicalId":50731,"journal":{"name":"Biochimica et Biophysica Acta-Bioenergetics","volume":"1866 3","pages":"Article 149559"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “New insights into the involvement of residue D1/V185 in Photosystem II function in Synechocystis 6803 and Thermosynechococcus vestitus” [Biochim. Biophys. Acta Bioenerg. 1866 (2025) 149550]","authors":"Alain Boussac ,&nbsp;Julien Sellés ,&nbsp;Tania Tibiletti ,&nbsp;Miwa Sugiura ,&nbsp;Robert L. Burnap","doi":"10.1016/j.bbabio.2025.149558","DOIUrl":"10.1016/j.bbabio.2025.149558","url":null,"abstract":"","PeriodicalId":50731,"journal":{"name":"Biochimica et Biophysica Acta-Bioenergetics","volume":"1866 3","pages":"Article 149558"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expansion microscopy reveals thylakoid organisation alterations due to genetic mutations and far-red light acclimation","authors":"Jarne Berentsen,&nbsp;Peter R. Bos,&nbsp;Emilie Wientjes","doi":"10.1016/j.bbabio.2025.149552","DOIUrl":"10.1016/j.bbabio.2025.149552","url":null,"abstract":"<div><div>The thylakoid membrane is the site of the light-dependent reactions of photosynthesis. It is a continuous membrane, folded into grana stacks and the interconnecting stroma lamellae. The CURVATURE THYLAKOID1 (CURT1) protein family is involved in the folding of the membrane into the grana stacks. The thylakoid membrane remodels its architecture in response to light conditions, but its 3D organisation and dynamics remain incompletely understood. To resolve these details, an imaging technique is needed that provides high-resolution 3D images in a high-throughput manner. Recently, we have used expansion microscopy, a technique that meets these criteria, to visualise the thylakoid membrane isolated from spinach. Here, we show that this protocol can also be used to visualise enveloped spinach chloroplasts. Additionally, we present an improved protocol for resolving the thylakoid structure of <em>Arabidopsis thaliana</em>. Using this protocol, we show the changes in thylakoid architecture in response to long-term far-red light acclimation and due to knocking out CURT1A. We show that far-red light acclimation results in higher grana stacks that are packed closer together. In addition, the distance between stroma lamellae, which are wrapped around the grana, decreases. In the <em>curt1a</em> mutant, grana have an increased diameter and height, and the distance between grana is increased. Interestingly, in this mutant, the stroma lamellae occasionally approach the grana stacks from the top. These observations show the potential of expansion microscopy to study the thylakoid membrane architecture.</div></div>","PeriodicalId":50731,"journal":{"name":"Biochimica et Biophysica Acta-Bioenergetics","volume":"1866 3","pages":"Article 149552"},"PeriodicalIF":3.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}