C. Salazar, F. Landeros, E. Bustos, Paulina B. Bravo, C. Pérez, J. Becerra, Darcy Ríos, M. Uribe
{"title":"Propagation and Bulblet Enhancement of Rhodophiala pratensis from Seeds Germinated In Vitro","authors":"C. Salazar, F. Landeros, E. Bustos, Paulina B. Bravo, C. Pérez, J. Becerra, Darcy Ríos, M. Uribe","doi":"10.7764/RCIA.V46I1.1847","DOIUrl":"https://doi.org/10.7764/RCIA.V46I1.1847","url":null,"abstract":"Rhodophiala pratensis is an endemic geophyte from Chile with great importance to the national market of cut flowers and potted and garden plants. However, many geophytes have conservation issues and are considered endangered species. Efficient and controlled propagation techniques have been useful for the commercial production of this species and for its conservation. A micropropagation protocol using seeds was developed to determine an optimum method to increase bulb production rates and plant production. The effect of sucrose levels in the medium on the growth of bulbs was tested in vitro (30, 60 and 90 g L -1 ). Then, the effects on shoot and bulb induction of the cytokinin BAP at concentrations of 1.0, 2.0 and 4.0 mg L -1 with the auxin NAA at concentrations of 0.1, 1.0 and 2.0 mg L -1 were analyzed. The germination rate after eight weeks increased to 86% with very low contamination (7%). Bulb growth increased when high levels of sucrose were added to the medium; the optimum sucrose concentration was 90 g L -1 . Acclimatization was successful, with a plant survival rate of 63% and an average bulb growth of 0.5 cm. This is the first study to demonstrate the possibility of propagating R. pratensis seeds in vitro.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46537140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Pizarro-Araya, F. M. Alfaro, Rodrigo A. Muñoz-Rivera, Juan E. Barriga-Tuñón, L. Letelier
{"title":"Arthropods of forestry and medical-veterinary importance in the Limarí basin (Coquimbo region, Chile)","authors":"J. Pizarro-Araya, F. M. Alfaro, Rodrigo A. Muñoz-Rivera, Juan E. Barriga-Tuñón, L. Letelier","doi":"10.7764/RCIA.V46I1.1907","DOIUrl":"https://doi.org/10.7764/RCIA.V46I1.1907","url":null,"abstract":"The Limari River valley, located in the Coquimbo Region of Chile, is an important area for agricultural production that pertains to the transverse valleys ecoregion, known as Norte Chico. In recent decades, the continuous expansion of agriculture towards the dryland areas has favored the introduction and establishment of potential pests, such as arthropods, that may affect forestry crops and transmit zoonotic diseases. We analyzed the arthropod assemblages that were of forestry and medical-veterinary importance in the Limari basin. After reviewing historical data, specimen collections, and the available literature, a total of 10 families, 39 genera, and 51 species of arthropods of forestry importance were recorded, 43.1% of which were of quarantine importance. Xylophagous groups of quarantine importance to the US included Cryptotermes brevis (Kalotermitidae), Neoterius mystax (Bostrichidae), Tyndaris planata (Buprestidae), Rhyephenes humeralis (Curculionidae) and Phoracantha recurva (Cerambycidae). Other quarantine insects of forestry importance recorded in the area included were Micrapate scabrata and Polycaon chilensis (Bostrichidae). In addition, the groups of medical-veterinary importance were represented by 11 families, 14 genera, and 18 species, 9 of which were arachnids and 9 were insects. Among the identified arachnids, the most important genera were Loxosceles (Sicariidae), Latrodectus , Steatoda (Theridiidae) and Rhipicephalus (Ixodidae), whereas among the identified insects, the most important were Triatoma and Mepraia (Reduviidae), Pediculus (Pediculidae), and Ceratophyllus (Ceratophyllidae). We hope this study serves as a basis for the development of forest pest monitoring and control programs as well as an understanding of the potential risks posed by zoonotic agents and their implications for public health.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46894283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Nuñez-Guerrero, Elizabeth Salazar-Vázquez, J. Páez-Lerma, R. Rodríguez‐Herrera, N. Soto-Cruz
{"title":"Physiological characterization of two native yeasts in pure and mixed culture using fermentations of agave juice","authors":"M. Nuñez-Guerrero, Elizabeth Salazar-Vázquez, J. Páez-Lerma, R. Rodríguez‐Herrera, N. Soto-Cruz","doi":"10.7764/RCIA.V46I1.1880","DOIUrl":"https://doi.org/10.7764/RCIA.V46I1.1880","url":null,"abstract":"Yeast cells are subjected to diverse environmental conditions during the alcoholic fermentation of agave juice, causing different kinetic behaviors. Agave juice was used as culture medium to evaluate the kinetic behavior of Saccharomyces cerevisiae ITD-00185 and Torulaspora delbrueckii ITD-00014a, as pure and mixed cultures, under different inoculum sizes (1×10 5 , 1×10 6 , 1×10 7 and 1×10 8 cells mL -1 ), and combined pH levels (3.5, 4.0 and 4.5) and temperatures (18 °C, 28 °C and 38 °C). Saccharomyces cerevisiae displayed high fermentation capacities at all inoculum concentrations assayed. However, T. delbrueckii required a high inoculum concentration (≥1×10 7 cells mL -1 ) to perform at fermentation levels similar to S. cerevisiae . Low temperatures (18 °C) slowed fermentation, while high temperatures (38 °C) adversely affected the development of the yeast strains, especially T. delbrueckii . The best temperature was 28 °C in all fermentations. The pH level had a strong effect on the performance of the coculture, since the fermentation kinetics suggested a synergistic effect at pH 4.5, while an antagonistic effect was postulated at pH 3.5. In all of the mixed culture cases, a positive effect at 28 °C, especially at pH 4.0 and 4.5, was demonstrated by greater levels of sugar consumption and ethanol production (~20%, p<0.05) compared to fermentations of the S. cerevisiae monoculture. The coculture results allow us to postulate that a complex interaction exists between the two yeasts, which could be synergistic or antagonistic, as the environmental conditions change.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47693291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Ochoa, A. Hernández, J. Delgado, Mariana Beltran, L. M. Tapia, Omegar Hernandez, E. Cerna
{"title":"First report of avocado tree wilt by Pythium sp. amazonianum in Mexico","authors":"Y. Ochoa, A. Hernández, J. Delgado, Mariana Beltran, L. M. Tapia, Omegar Hernandez, E. Cerna","doi":"10.7764/RCIA.V45I3.1905","DOIUrl":"https://doi.org/10.7764/RCIA.V45I3.1905","url":null,"abstract":"In August and September 2014, different avocado orchards ( Persea americana Mill. Var. Hass) were sampled in four municipalities (Tancitaro, Uruapan, San Juan Nuevo Parangaricutiro and Periban) of the “Franja aguacatera” in Michoacan State, Mexico. Samples were collected from avocado trees showing symptoms of the “Tristeza del aguacate” disease. Strains of Pythium sp. were obtained consistently from symptomatic tissue isolated in the selective medium V8®-Agar-PARPH. Morphological identification was made through taxonomic keys, and molecular identification was obtained by sequencing Internal Transcribed Spacer (ITS) gene. Koch’s postulates were corroborated by pathogenicity tests carried out on P. americana seedlings with six true leaves, identifying the strain of P. sp. amazonianum as the causal agent of the “Tristeza del aguacate” disease. This is the first report that associates the “Tristeza del aguacate” with P. sp. amazonianum in Michoacan, Mexico.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41382266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. T. Oliveira, Gilka T Silva, Williane P. S. Diniz, Everthon F Figueredo, I. B. Santos, Danubia Ramos Moreira de Lima, Maria Caroline Quecine Verdi, J. Kuklinsky-Sobral, F. J. Freire
{"title":"Diazotrophic bacteria isolated from Brachiaria spp.: genetic and physiological diversity","authors":"J. T. Oliveira, Gilka T Silva, Williane P. S. Diniz, Everthon F Figueredo, I. B. Santos, Danubia Ramos Moreira de Lima, Maria Caroline Quecine Verdi, J. Kuklinsky-Sobral, F. J. Freire","doi":"10.7764/RCIA.V45I3.1949","DOIUrl":"https://doi.org/10.7764/RCIA.V45I3.1949","url":null,"abstract":"Grass from the genus Brachiaria spp. predominates in pastures with low fertile soils. This scenario highlights the importance of the association with microorganisms to foster plant growth, which becomes essential to the successful establishment of this forage in such environments. This study aimed to evaluate the genetic variability and identify the mechanisms of plant growth promotion, in vitro, of bacteria associated with Brachiaria decumbens Stapf. and Brachiaria humidicola (Rendle.) Schweickerdt in Pernambuco, Brazil. We evaluated 20 isolates of diazotrophic bacteria obtained from the endophyte or rhizosphere communities. The genetic characteristics were determined via sequencing the 16S rRNA region, which allowed us to identify ten different bacterial genera: Bacillus sp., Burkholderia sp., Enterobacter sp., Klebsiella sp., Microbacterium sp., Pantoea sp., Ralstonia sp., Rhizobium sp., Sinomonas sp., and Sphingomonas sp., with a specificity of the genus Rhizobium sp. to Brachiaria decumbens Stapf.. The phenotypic and functional characteristics revealed that 100% of the bacterial strains produced indol-3-acetic acid (IAA) with the addition of L-tryptophan, and 60% presented IAA production independent of the L-tryptophan pathway. We also detected that 70% of the isolated bacteria possessed the capacity to solubilize phosphorus. The analysis of the enzymatic output revealed that 30% of the bacterial isolates produced cellulase, 60% produced pectate lyase, 15% produced polygalacturonase, and 30% produced amylase. We also detected the production of N-acyl homoserine lactones in 65% of bacterial strains. In summary, our results showed that plants of B. decumbens Stapf. and B. humidicola (Rendle.) Schweickerdt interacted with different bacterial genera capable of promoting plant growth.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46122425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Peri-urban family farming and agricultural earnings: The effect of long-term participation in an extension program in a metropolitan area","authors":"Sofia Boza, R. Jara‐Rojas","doi":"10.7764/RCIA.V45I3.1783","DOIUrl":"https://doi.org/10.7764/RCIA.V45I3.1783","url":null,"abstract":"The objective of this paper is to assess the effect of long-term participation in an extension program on peri-urban family farming earnings. For this purpose, a survey of a sample of 156 participants in the Local Development Program (PRODESAL) of the Chilean National Institute for Agricultural Development (INDAP) was conducted in the Metropolitan Region of Santiago. Half of the respondents were long-term participants with at least four yrs in the program, and the rest of the farmers were one-yr beneficiaries. A treatment regression model (TRM) with a binary endogenous variable was used to identify differences among long- and short-term farmer participation in PRODESAL. The first stage of the model is a probit regression that identifies factors influencing long-term participation in PRODESAL. The second stage has the total value of production (TVP) as a dependent variable, which captures agricultural earnings, and one of the explanatory variables is the binary endogenous regressor of the first stage of the model. The results show a positive impact of long-term participation in PRODESAL on TVP. Other variables, such as farm size, fertilization and access to credit, were significant and positively related to TVP as well. Education and social capital are shown to be variables that increase the probability of long-term participation of family farmers in the program.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48435262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Altop, I. Coskun, G. Filik, Altug Kuçukgul, Y. Bekiroğlu, H. Cayan, Emrah Güngör, A. Sahin, G. Erener
{"title":"Amino acid, mineral, condensed tannin, and other chemical contents of olive leaves (Olea europaea L.) processed via solid-state fermentation using selected Aspergillus niger strains","authors":"A. Altop, I. Coskun, G. Filik, Altug Kuçukgul, Y. Bekiroğlu, H. Cayan, Emrah Güngör, A. Sahin, G. Erener","doi":"10.7764/RCIA.V45I3.1886","DOIUrl":"https://doi.org/10.7764/RCIA.V45I3.1886","url":null,"abstract":"The present study aimed to examine the effects of solid-state fermentation (SSF) using selective A. niger strains on the amino acid, mineral, condensed tannin, and other chemical contents of olive leaves. The dried samples were divided into nonfermented (C) and fermented (F) olive leaves, and the latter were fermented by the following A. niger strains: ATCC® 9142TM (F1), ATCC® 200345TM (F2), ATCC® 52172TM (F3), and ATCC® 201572TM (F4), with three replicates for each treatment. Group F4 presented the best results, although all fermented groups generally presented higher performance than C. The total content of amino acids of the fermented olive leaves increased by 68–209% in comparison to that of C, while the cellulose content of the fermented olive leaves decreased by 7–25%. The ash, crude protein (CP), and ether extract (EE) contents increased after fermentation, but the crude fiber (CF) and nitrogen-free extract (NFE) contents decreased. The content of neutral detergent fiber (NDF) did not change, but acid detergent fiber (ADF) varied among the groups. The starch and sugar contents of all fermented groups except F1 also decreased compared to those of C. The mineral contents increased in all fermented groups, and the condensed tannin content varied according to the A. niger strain used. Thus, olive leaves fermented with different A. niger strains, especially F4, seem to have considerable potential as ruminant feed, as they are enriched with amino acids and minerals and have an improved chemical composition. However, these results should be supported and validated by animal experiments.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47858523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. M. D. O. M. Carias, J. H. Guilhen, Tiago de Souza Marçal, Adésio Ferreira, M. F. S. Ferreira
{"title":"Genetic divergence toward the selection of promising bean progenitors via mixed multivariate models","authors":"C. M. D. O. M. Carias, J. H. Guilhen, Tiago de Souza Marçal, Adésio Ferreira, M. F. S. Ferreira","doi":"10.7764/RCIA.V45I3.1902","DOIUrl":"https://doi.org/10.7764/RCIA.V45I3.1902","url":null,"abstract":"The genetic variability present in the bean ( Phaseolus vulgaris L.) germplasm that is currently used as an agricultural crop has been shown to be stable in production and is acceptable for human sustenance. Accordingly, to maintain as much of the available variability as possible, this study aimed to examine the genetic divergence in the bean using multivariate analysis to identify the sources of genetic variability and enable breeders to recognize genetic combinations that have a greater chances of success before crossings are performed. This study was conducted in a randomized block design with three replications in the agricultural year 2015. The agronomic traits evaluated were the stem diameter (DIAM) in millimeters, plant height (PH) in centimeters, number of seedsper plant (NS), protein percentage (PROT), height of the first pod (HFP) in centimeters, pod number (PN), grain mass per plant (GM) in g plant-1, grain yield (GY) in kg ha-1, and straw yield (SY) in kg ha-1. To enable selection of the most divergent genotypes, twenty different genotypes were analyzed via clustering according to the average linkage criterion (UPGMA) using a matrix of the mean standardized Euclidean distances and principal component analysis based on the values predicted via a multivariate mixed model. The results obtained in this study revealed a high degree of genetic divergence and allowed the progenies to be allocated into different groups, as well as recommended crossings for future bean breeding programs.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45597875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrés Chiang Guzmán, B. Schnettler, M. Mora, Mauricio Aguilera
{"title":"Perceived quality of and satisfaction from sweet cherries (Prunus avium L.) in China: Confirming relationships through structural equations","authors":"Andrés Chiang Guzmán, B. Schnettler, M. Mora, Mauricio Aguilera","doi":"10.7764/RCIA.V45I3.1930","DOIUrl":"https://doi.org/10.7764/RCIA.V45I3.1930","url":null,"abstract":"In recent years, Chile has been the Chinese market’s main supplier of fresh sweet cherries. The aim of this study was to develop a relational model with two working hypotheses to model Chinese consumer satisfaction with sweet cherries as a result of the quality perceived by intrinsic and extrinsic attributes. One hundred and ninety-three buyers in the Sanyuanli Market in the Chaoyang District, Beijing, were surveyed using closed-ended questions on a Likert scale. To develop a model that fulfilled the indicated objective, an exploratory factor analysis and a subsequent system of structural equations were used to confirm the relationships, presenting an adequate goodness-of-fit test to accept the model according to the literature. Among the main results, it was determined that consumer satisfaction with sweet cherries is explained by the perceived intrinsic quality, where medium size and color are the determining attributes, and the product price, Chilean origin and delivery time explain the perceived extrinsic quality (critical ratio of 2.937 and p-value of 0.003). Finally, the intrinsic quality positively and significantly influences Chinese consumer satisfaction with sweet cherries, in contrast to the results for perceived extrinsic quality, which are not statistically significant.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.7764/RCIA.V45I3.1930","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43245434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gustavo Cruz Madariaga, F. Rodríguez, P. Tapia, H. Bown
{"title":"Growth responses after a future crop tree thinning and a thinning from below in a second-growth Nothofagus pumilio forest in Tierra del Fuego, Chile","authors":"Gustavo Cruz Madariaga, F. Rodríguez, P. Tapia, H. Bown","doi":"10.7764/RCIA.V45I3.1856","DOIUrl":"https://doi.org/10.7764/RCIA.V45I3.1856","url":null,"abstract":"Nothofagus pumilio (lenga) is the most important native species for timber production in southern Patagonia both in Chile and Argentina. Thinning application to second-growth N. pumilio forests has been limited in Patagonia, probably because of the long time necessary for the investment to be recovered. In addition, experimental trials have focused mostly on thinnings from below, leaving a high residual stand density, which has led to a modest growth response. Using measurements (1995–2014) obtained from a thinning trial carried out in a second-growth N. pumilio forest located in Tierra del Fuego (Chile), the objective of this case study was to analyze whether selective thinnings with future crop trees (in the sense of Z-Baumen thinning) promote greater growth responses than those from a thinning from below. In addition, we explored the associations between climatic factors and age on N. pumilio tree growth. Trial treatments comprised a control, a thinning from below and a future crop tree thinning. The growth response after a future crop tree thinning was greater and lasted for a longer period (10 yrs) than did the thinning from below (4 yrs). The tree basal area and diameter at breast height (dbh) growth increased with dbh and annual absolute minimum temperature and decreased with age for the unthinned treatment during the period from 1977–2014 (~70–110 yrs old). Our results suggest that the future crop thinning might be a better alternative than the thinning from below for secondary N. pumilio forests in Patagonia due to the greater growth response while being a less intensive silvicultural method.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44487868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}