Theoretical and Natural Science最新文献_第5页

The mechanism and eciency of mRNA-based dendritic cell immunotherapy 基于 mRNA 的树突状细胞免疫疗法的机制和有效性

Theoretical and Natural Science Pub Date : 2024-03-08 DOI: 10.54254/2753-8818/33/20240543

Jiayi Wang

引用次数: 0

Prognostic value of mitophagy gene PINK1 in triple-negative breast cancer 有丝分裂吞噬基因 PINK1 在三阴性乳腺癌中的预后价值

Theoretical and Natural Science Pub Date : 2024-03-08 DOI: 10.54254/2753-8818/33/20240908

Zengjian Tian, Shilong Yu, Yongzhao Zhu

{"title":"Prognostic value of mitophagy gene PINK1 in triple-negative breast cancer","authors":"Zengjian Tian, Shilong Yu, Yongzhao Zhu","doi":"10.54254/2753-8818/33/20240908","DOIUrl":"https://doi.org/10.54254/2753-8818/33/20240908","url":null,"abstract":"Mitochondrial autophagy plays a crucial role in the development, progression, and treatment of triple-negative breast cancer (TNBC). A total of 43 genes related to mitochondria were obtained from the scientific literature in Pubmed. To assess the expression of these genes, they were categorized into high and low-scoring groups using the ssGSEA algorithm. Additionally, the infiltration of immune cells in the combined dataset was evaluated using the CIBERSORTx algorithm. To analyze the expression of the selected genes, one-way Cox regression analysis, along with clinical staging variables, was employed. A multifactorial Cox regression model was constructed, and a forest plot was generated to assess the predictive power of the model on the actual outcomes. The accuracy of the model was confirmed by validating the expression of the PINK1 gene and its model through immunohistochemistry. TNBC cancer was categorized into two molecular subtypes (cluster1 and cluster2) based on the MRGs. Finally, a prognostic model was developed using the PINK1 gene, which demonstrated a considerable degree of accuracy. The validity of the PINK1 gene and its model was confirmed through immunohistochemistry, indirectly indicating that PINK1 might serve as a potential therapeutic target and prognostic biomarker for TNBC.","PeriodicalId":506830,"journal":{"name":"Theoretical and Natural Science","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140076972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies of SUD and MDDs effect on decision-making based on the drift diffusion model 基于漂移扩散模型的 SUD 和 MDD 对决策影响的研究

Theoretical and Natural Science Pub Date : 2024-03-08 DOI: 10.54254/2753-8818/33/20240839

Patrick Mukuo Luo

引用次数: 0

Study on breast cancer image detection and classification based on residual connected convolutional neural network (CNN) 基于残差连接卷积神经网络（CNN）的乳腺癌图像检测与分类研究

Theoretical and Natural Science Pub Date : 2024-03-08 DOI: 10.54254/2753-8818/33/20240914

Chengxiao Dai

{"title":"Study on breast cancer image detection and classification based on residual connected convolutional neural network (CNN)","authors":"Chengxiao Dai","doi":"10.54254/2753-8818/33/20240914","DOIUrl":"https://doi.org/10.54254/2753-8818/33/20240914","url":null,"abstract":"This paper presents a method for breast cancer image detection and classification based on convolutional neural networks. While traditional breast cancer detection methods carry the risk of subjectivity and misclassification, machine learning can improve the accuracy and efficiency of detection by training algorithms to automatically identify breast cancer lesions in patients. The dataset of this paper includes 7909 microscopic images of breast tumor tissues with different magnifications collected from 82 patients, which are divided into two categories: benign and malignant tumors. In this paper, residual connection is designed for convolutional neural networks, which is a cross-layer connection method that can effectively solve the gradient vanishing and gradient explosion problems in deep neural networks, and at the same time, improve the generalization ability and training speed of the model. In this paper, the dataset is divided into training set, validation set and test set according to the ratio of 6:2:2. Through training and validation, the prediction accuracy of the obtained model on the test set reaches 85.5%, which achieves good prediction results in breast cancer image detection and classification. Eventually, the model's loss converged at 0.53 and the AUC was 0.832. The research results in this paper are of great significance for the early diagnosis and treatment of breast cancer. Automatic identification and classification of breast cancer images by machine learning algorithms can reduce the subjective judgment of doctors, improve the accuracy and efficiency of detection, and provide important support for the early diagnosis and treatment of breast cancer. Meanwhile, the research method and results of this paper also provide reference and reference for image detection and classification of other malignant tumors.","PeriodicalId":506830,"journal":{"name":"Theoretical and Natural Science","volume":"42 25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140076975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of buffer pH on enzyme activity 缓冲液 pH 值对酶活性的影响

Theoretical and Natural Science Pub Date : 2024-03-08 DOI: 10.54254/2753-8818/33/20240893

Yixuan Gu

{"title":"The effect of buffer pH on enzyme activity","authors":"Yixuan Gu","doi":"10.54254/2753-8818/33/20240893","DOIUrl":"https://doi.org/10.54254/2753-8818/33/20240893","url":null,"abstract":"This study investigates the effect of pH on enzyme-catalyzed activity, with an emphasis on the involvement of wheat germ acid phosphatase in dephosphorylation processes. Temperature, pH, and enzyme concentration all have a substantial impact on enzyme activity and substrate interaction. Wheat germ acid phosphatase was chosen for this work. The activity of the enzyme in dephosphorylating p-nitrophenyl phosphate (p-NPP) into p-nitrophenol (p-NP) was evaluated using a stopped assay approach. The reaction was stopped by introducing sodium hydroxide, which ionises p-NP and produces a yellow anion visible at 405 nm. The experiment measured product production in nmol per minute and included three sections: Created a standard for the amount of p-NP with its absorbance at 405 nm; Found the ideal incubation period for maximal product generation by wheat germ acid phosphatase; Evaluated the effect of pH on enzyme activity measuring the first reaction velocity in various pH settings. The results showed that when the buffer pH diverged from the ideal value of 5.5, catalysis rates reduced dramatically, implying that high pH levels may modify the protein structure of the enzyme and reduce its catalytic effectiveness. This work emphasises the importance of pH in enzymatic activity and its potential impacts on enzyme structural integrity.","PeriodicalId":506830,"journal":{"name":"Theoretical and Natural Science","volume":"51 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140077114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diacylglycerol oil on metabolic syndrome: A Review 二酰甘油对代谢综合征的影响：综述

Theoretical and Natural Science Pub Date : 2024-03-08 DOI: 10.54254/2753-8818/33/20240389

Yilin Lyu

引用次数: 0

Gene sequencing technologies and their application in cancer research 基因测序技术及其在癌症研究中的应用

Theoretical and Natural Science Pub Date : 2024-03-08 DOI: 10.54254/2753-8818/33/20240885

Yingxun Liu

引用次数: 0

Exploration and determination of lung cancer targets using multi-omics approaches 利用多组学方法探索和确定肺癌靶点

Theoretical and Natural Science Pub Date : 2024-03-08 DOI: 10.54254/2753-8818/33/20240767

Chenyu Liang, Pinyan Rosemary Duan

引用次数: 0

The role of protein kinase A signaling in cardiomyopathy 蛋白激酶 A 信号在心肌病中的作用

Theoretical and Natural Science Pub Date : 2024-03-08 DOI: 10.54254/2753-8818/33/20240895

Acacia Yuan

{"title":"The role of protein kinase A signaling in cardiomyopathy","authors":"Acacia Yuan","doi":"10.54254/2753-8818/33/20240895","DOIUrl":"https://doi.org/10.54254/2753-8818/33/20240895","url":null,"abstract":"Cardiomyopathy, a diverse group of heart muscle diseases, poses a significant challenge to global cardiovascular health. However, the intricate molecular mechanisms underlying cardiomyopathy, especially the pivotal role of protein kinase A (PKA) signaling pathways in its pathogenesis, remains unclear. The review synthesizes current research findings and highlights the multifaceted interactions between PKA and key cellular components implicated in cardiomyopathy progression. Moreover, this paper unfolds with an exploration of the normal physiological functions of PKA in cardiac cells, emphasizing its role in regulating crucial processes such as excitation-contraction coupling and cellular energy homeostasis. The article navigates through the dysregulation of PKA signaling observed in various forms of cardiomyopathy, including hypertrophic, dilated, and restrictive subtypes. Insightful analyses into the impact of PKA-mediated phosphorylation events on cardiac contractility, ion channel function, and mitochondrial dynamics shed light on the intricate interplay between signaling cascades and disease manifestation. Future clinical trials targeting the PKA signaling pathway are required to seek more effective and precise therapeutic approaches of cardiomyopathy.","PeriodicalId":506830,"journal":{"name":"Theoretical and Natural Science","volume":"114 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140257041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research and future directions of HPV-related vaccines 人类乳头瘤病毒相关疫苗的研究和未来方向

Theoretical and Natural Science Pub Date : 2024-03-08 DOI: 10.54254/2753-8818/33/20240903

Xiaofei Wu

{"title":"Research and future directions of HPV-related vaccines","authors":"Xiaofei Wu","doi":"10.54254/2753-8818/33/20240903","DOIUrl":"https://doi.org/10.54254/2753-8818/33/20240903","url":null,"abstract":"Human papillomavirus (HPV) infection is a major cause of cervical lesions such as cervical cancer in women. Prophylactic vaccines developed against HPV have been widely used, but these vaccines are only effective against certain types of HPV and do not work in people who are already infected. Therefore, the development of therapeutic vaccines is also particularly important. The use of the modified poxvirus (MVA) as a vector for HPV vaccinations has garnered attention in recent years. It has been found that modified poxvirus vaccines are able to combat human papillomavirus infection and have significant safety and efficacy profiles. Based on the study of the distribution of HPV types in different regions and populations, this paper finds that there are differences in the prevalence of high-risk HPV subtypes in different regions, and that a large number of infected people need more effective treatments. At the same time, by comparing the advantages and disadvantages of preventive and therapeutic vaccines, we recognize that both have roles that cannot be replaced by the other in the task of fighting HPV, and that the two vaccines need to be developed together and complement each other in order to minimize the harm of HPV. Based on these findings, this paper provides a constructive reference for future targeted prevention and treatment of HPV in different regions of the world with different HPV distributions.","PeriodicalId":506830,"journal":{"name":"Theoretical and Natural Science","volume":"99 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140257065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0