Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale最新文献

{"title":"Reduced levels of genomic damage in young martial artists","authors":"Alessandro Nota, Mattia Lambertini, A. Santovito","doi":"10.4081/jbr.2024.11678","DOIUrl":"https://doi.org/10.4081/jbr.2024.11678","url":null,"abstract":"The impact of physical activity on the levels of genomic damage is still poorly understood. In this work, we aimed to investigate the influence of a constant martial arts training on the levels of DNA damage. Moreover, we explored the possible association between genomic damage and single-gene polymorphisms on metabolic and DNA repair genes. To do this, we compared the frequency of micronuclei and other nuclear aberrations in the buccal mucosa cells of 35 healthy, young martial artists with 35 healthy, young sedentary controls. Additionally, we genotyped all participants for three metabolic and two DNA repair genes to evaluate the impact of the relative single-gene polymorphisms on DNA damage. Genomic damage was significantly lower in athletes than in sedentary controls, as evidenced by a decrease in both micronuclei and total aberrations. Instead, single-gene polymorphisms did not significantly alter the frequencies of aberrations. These findings suggest that training may have a protective effect against genomic damage, supporting the health benefits provided by physical activity. The influence of other factors should be considered, and further studies focusing on disciplines with different training regimes are necessary to evaluate thoroughly their influence on genomic damage.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"19 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140259911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the effect of brown rice, tuna, cucumber, and pineapple treatment on proteinuria and albuminuria in Wistar Rats strain with chronic kidney disease","authors":"Tasnim Tasnim, Selpirahmawati Saranani","doi":"10.4081/jbr.2024.11883","DOIUrl":"https://doi.org/10.4081/jbr.2024.11883","url":null,"abstract":"\u0000This study aims to determine how nutrition therapy with brown rice, tuna fish, pineapple, and cucumber affects proteinuria and albuminuria in male Wistar rats with chronic kidney disease (CKD). In this study, 16 male Wistar rats with CKD were divided into four groups. The result states that the third treatment, consisting of 341 g/kg BW (Body Weight)/day brown rice, 15 g/kg BW/day tuna fish, 2 g/kg BW/day pineapple, and 2 g/kg BW/day cucumber could reduce both proteinuria (p < 0.01) and albuminuria (p < 0.01) in rats. However, the first (250 g brown rice, 13 g/kg BW/day tuna fish, 1 g/kg BW/day pineapple, 1 g/kg BW/day cucumber) and second treatment (296 g/kg BW/day brown rice, 14 g/kg BW/day tuna fish, 1.5 g/kg BW/day pineapple, 1.5 g/kg BW/day cucumber) could not reduce the levels of proteinuria and albuminuria in rats. In conclusion, giving a balanced diet containing 341 g/kg BW/day of brown rice, 15 g/kg BW/day of tuna fish, 2 g/kg BW/day of pineapple, and 2 g/kg BW/day of cucumber for CKD can maintain body fluid and increase the re-absorption of proteins and albumin. Therefore, the third nutrition formula can be recommended to people with kidney disease after clinical tests on humans.\u0000","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"63 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140077665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring dimensions and shapes of edentulous patients consulting the prosthodontics centre at Ibn Sina University Hospital in Rabat, Morocco: a cross-sectional study","authors":"M. Azhari, Abdelkoui Anissa, Yahya Melainin, O. Bentahar, Faiza Benfdil","doi":"10.4081/jbr.2024.11507","DOIUrl":"https://doi.org/10.4081/jbr.2024.11507","url":null,"abstract":"This cross-sectional study conducted at the Prosthodontics Centre, Ibn Sina University Hospital in Rabat, Morocco, investigates the dimensions and shapes characterizing maxillary and mandibular edentulous arches in a patient population seeking consultation. The objective is to provide insights crucial for optimizing outcomes in prosthetic therapy, informing decisions on impression trays, occlusion planes, prosthetic teeth, and implant attributes. Sixty-seven edentulous Moroccan patients (40 men, 27 women), aged 31 to 90, were recruited at the Prosthodontics Centre. Maxillary and mandibular casts were meticulously crafted, and precise measurements of anterior and posterior width and length were acquired for both arches. Arch shapes were categorized into O-shape, V-shape, and U-shape. Data were analyzed using Microsoft® Excel 2013 and SPSS software (IBM SPSS Statistics 23). Analysis revealed maxillary arch lengths ranging from 40 to 58 mm, maxillary arch widths from 38 to 58 mm, mandibular arch lengths from 33 to 56 mm, and mandibular arch widths from 30 to 60 mm. The predominant arch shape was the ovoid, constituting 34% (n=23) of maxillary cases and 54% (n=36) of mandibular cases. This study provides novel insights into the dimensions and shapes of maxillary and mandibular edentulous arches in patients seeking consultation at Ibn Sina University Hospital, Rabat, Morocco. The findings offer valuable guidance for prosthetic therapy decisions, emphasizing the importance of integrating established references and arch classifications for optimal outcomes in the treatment of edentulous patients in Morocco.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"64 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140436900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview of glioblastoma multiforme in vitro experimental models","authors":"A. Vitale, Giuseppa D’Amico, Radha Santonocito, Gioacchino Spinnato, Martina Di Marco, Federica Scalia, C. Campanella, Giovanni Tringali, I. Giusti, V. Dolo, Francesco Cappello, Celeste Caruso Bavisotto","doi":"10.4081/jbr.2024.11920","DOIUrl":"https://doi.org/10.4081/jbr.2024.11920","url":null,"abstract":"Glioblastoma multiforme (GBM) is the most common primary brain tumor, characterized by a remarkable inner complexity and inter-tumor variability. Moreover, it is very aggressive and resistant to conventional treatments, so that it rapidly relapse. Therefore, there is an immediate need for experimental strategies to enhance our comprehension of GBM, aiming to mitigate its economic and social impact. Here, we described different in vivo and in vitro strategies currently used for the study of GBM. First, we gave a brief and general overview of the classical in vivo models, including xenograft mouse and zebrafish models and canine models, offering a wide range of advantages but also presenting a series of strong limitations. Thus, we described in vitro models, starting from more traditional 2D culture models, comparing different approaches and critically exposing the advantages and disadvantages of using one or the other methods. We also briefly described GBM 2D culture systems that allow recreating multiple cell-cell and cell-extracellular matrix contacts but still do not reflect the complexity of in vivo tumors. We finally described the intricacies of the more novel 3D in vitro models, e.g., spheroids and organoids. These sophisticated models have demonstrated exceptional suitability across a wide spectrum of applications in cancer research, ranging from fundamental scientific inquiries to applications in translational research. Their adaptability and three-dimensional architecture render them invaluable tools, offering new insights and paving the way for advancements in both basic and applied research.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"479 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139835725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary phytochemical screening, in vitro antioxidant activity and insecticidal activity of methanolic leaves extract of Cedrus atlantica from Belezma, Algeria","authors":"Ouafa Deffa, Badis Daikh","doi":"10.4081/jbr.2024.11668","DOIUrl":"https://doi.org/10.4081/jbr.2024.11668","url":null,"abstract":"This work was conceived in the context of valorisation of the endemic forest species Cedrus atlantica (Pinaceae family), widespread in the mountainous massif of eastern Algeria. This study aimed to investigate the antioxidant and insecticidal activity of the methanolic extract of the aerial part (leaves) of C. atlantica (CAMeOH). The extraction made it possible to obtain a yield of approximately 7.49% from 350 g of dry powdered plant material. The results of the phytochemical screening revealed the presence of tannins, alkaloids, terpenoids and flavonoids as major components. In vitro antioxidant evaluation was carried out using two different methods. The reducing power assay test result revealed an optical density (DO) value of 1.7±0.02 at a dose of 600 µg/mL of CAMeOH. Concerning the iron chelation activity, the recorded IC50 value was 62.12 μg/mL and R²=0.87. The insecticidal activity against the stored-food insect Tribolium confusum was evaluated using the contact treatment mode and showed a significant dose-dependent effect (P≤0.05) with a mortality rate of 98.32 ± 0.50 % at a dose of 50 mg/mL (after 96 hours of contact). The results of this study confirmed the potential antioxidant and insecticidal activity of the methanolic extract from the leaves of C. atlantica.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139774077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary phytochemical screening, in vitro antioxidant activity and insecticidal activity of methanolic leaves extract of Cedrus atlantica from Belezma, Algeria","authors":"Ouafa Deffa, Badis Daikh","doi":"10.4081/jbr.2024.11668","DOIUrl":"https://doi.org/10.4081/jbr.2024.11668","url":null,"abstract":"This work was conceived in the context of valorisation of the endemic forest species Cedrus atlantica (Pinaceae family), widespread in the mountainous massif of eastern Algeria. This study aimed to investigate the antioxidant and insecticidal activity of the methanolic extract of the aerial part (leaves) of C. atlantica (CAMeOH). The extraction made it possible to obtain a yield of approximately 7.49% from 350 g of dry powdered plant material. The results of the phytochemical screening revealed the presence of tannins, alkaloids, terpenoids and flavonoids as major components. In vitro antioxidant evaluation was carried out using two different methods. The reducing power assay test result revealed an optical density (DO) value of 1.7±0.02 at a dose of 600 µg/mL of CAMeOH. Concerning the iron chelation activity, the recorded IC50 value was 62.12 μg/mL and R²=0.87. The insecticidal activity against the stored-food insect Tribolium confusum was evaluated using the contact treatment mode and showed a significant dose-dependent effect (P≤0.05) with a mortality rate of 98.32 ± 0.50 % at a dose of 50 mg/mL (after 96 hours of contact). The results of this study confirmed the potential antioxidant and insecticidal activity of the methanolic extract from the leaves of C. atlantica.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"138 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139833587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview of glioblastoma multiforme in vitro experimental models","authors":"A. Vitale, Giuseppa D’Amico, Radha Santonocito, Gioacchino Spinnato, Martina Di Marco, Federica Scalia, C. Campanella, Giovanni Tringali, I. Giusti, V. Dolo, Francesco Cappello, Celeste Caruso Bavisotto","doi":"10.4081/jbr.2024.11920","DOIUrl":"https://doi.org/10.4081/jbr.2024.11920","url":null,"abstract":"Glioblastoma multiforme (GBM) is the most common primary brain tumor, characterized by a remarkable inner complexity and inter-tumor variability. Moreover, it is very aggressive and resistant to conventional treatments, so that it rapidly relapse. Therefore, there is an immediate need for experimental strategies to enhance our comprehension of GBM, aiming to mitigate its economic and social impact. Here, we described different in vivo and in vitro strategies currently used for the study of GBM. First, we gave a brief and general overview of the classical in vivo models, including xenograft mouse and zebrafish models and canine models, offering a wide range of advantages but also presenting a series of strong limitations. Thus, we described in vitro models, starting from more traditional 2D culture models, comparing different approaches and critically exposing the advantages and disadvantages of using one or the other methods. We also briefly described GBM 2D culture systems that allow recreating multiple cell-cell and cell-extracellular matrix contacts but still do not reflect the complexity of in vivo tumors. We finally described the intricacies of the more novel 3D in vitro models, e.g., spheroids and organoids. These sophisticated models have demonstrated exceptional suitability across a wide spectrum of applications in cancer research, ranging from fundamental scientific inquiries to applications in translational research. Their adaptability and three-dimensional architecture render them invaluable tools, offering new insights and paving the way for advancements in both basic and applied research.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"32 33","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139775897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regional nutritional profile and antioxidant activity of Gelidium sesquipedale from the Moroccan Atlantic coast","authors":"Samira El Majnaoui, Mounia Lekrati, Said El Broudi, A. Belmouden, Abdellah El Houari, Nadia El Kadmiri","doi":"10.4081/jbr.2024.11859","DOIUrl":"https://doi.org/10.4081/jbr.2024.11859","url":null,"abstract":"Gelidium sesquipedale is a red seaweed exploited in Morocco for its agar-agar quality. Samples were examined across the Moroccan Atlantic coast to evaluate their nutritional composition and antioxidant activity. The objective was to find out the regional impact on the biochemical properties of this seaweed. The obtained results have shown that the region factor does not affect the carbohydrate content. However, protein content was higher in the western sites (15.77% in Lahdida and 16.79% in Sidi-Rahal) compared to those from the southern sites (14.08% in Sidi El Ghazi and 14.76% in Amgriou). Regarding the phenolic contents, the results are similar in the west (5.69 and 5.38 mg gallic acid equivalent (GAE)/g respectively in Sidi-Rahal and Lahdida) but differ in the south (6.22 mg GAE /g in Amgriou and 4.78 mg GAE /g in Sidi El Ghazi) due to stranding phenomena in Amgriou. Moreover, this seaweed exhibits the highest radical scavenging capacity (DPPH) and Ferric Reducing Antioxidant Power (FRAP) values in the south (90.41 and 114.33 µg/mL in Amgriou; 103.76 and 110.5 µg/mL in Sidi El Ghazi). Hence, the exploitation of Gelidium sesquipedale harvested on the western and southern coasts of Morocco should extend beyond the sole production of agar-agar.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"53 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140483216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytokine profile depending on titers of anti-SARS-CoV-2 IgG in the blood plasma of healthy volunteers","authors":"D. Krenytska, A. Rachkovska, V. Karbovskyy, N. Raksha, T. Vovk, O. Savchuk, Ludmila Ostapchenko, T. Halenova","doi":"10.4081/jbr.2023.11256","DOIUrl":"https://doi.org/10.4081/jbr.2023.11256","url":null,"abstract":"Post-COVID-19 conditions are the subject of extensive scientific study. It is known that system disorders may persist in people who recover from COVID-19 for 3 to 12 months (in most cases). The first line of systemic disorders is dysfunction of the immune system, especially the cytokine profile. The mechanism of the launch of immune complications is currently unknown. We hypothesized that anti-SARS-CoV-2 IgG might trigger the development of post-COVID-19 complications. Our research aimed to study the cytokine profile (including IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12β, TNF-α, IFN-γ) and the transcription factor NF-kB in the blood plasma of donor groups depending on various titers of anti-SARS-CoV-2 IgG. We determined the qualitative values of the cytokine parameters using ELISA (Enzyme Linked Immunosorbent Assay). We estimated the changes in the cytokine profiles in all donor groups. Differences between donor groups were established for each research parameter. We detected changes in NF-κB levels as a key transcription factor for the expression of inflammatory cytokines. The highest level was found in the donor group with a titer of anti-SARS-CoV-2 IgG 75 ± 5 Index (S/C). We detected some differences between donor groups in the levels of pro-inflammatory cytokines compared to a reference point. IL-1β, IL-6, IL-8, INF-γ, and TNF-α levels changed in most donor groups with titers of anti-SARS-CoV-2 IgG ≥ 10 ± 3 Index (S/C). In addition, we showed that the decrease in anti-inflammatory cytokine IL-4 in donor groups ranged from the titer of anti-SARS-CoV-2 IgG 55 ± 5 Index (S/C) to 85 ± 5 Index (S/C), but these changes for the anti-inflammatory cytokine IL-10 were not detected. We found that the cytokine profile may be affected during post-COVID-19 conditions. The cytokine profiles of the donor groups changed depending on the titers of anti-SARS-CoV-2 IgG. The obtained results testify to the fact that the antibody subpopulation may be a key reason for immune dysfunction, influencing the development of other pathological processes.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"115 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139174534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the interplay of phytohormones and polyamines in drought-stressed Cress (Lepidium sativum L.) leaves","authors":"Khalid Y. Alsharafa","doi":"10.4081/jbr.2023.11706","DOIUrl":"https://doi.org/10.4081/jbr.2023.11706","url":null,"abstract":"The study investigated prolonged drought stress effects on phytohormone and polyamine levels in cress (Lepidium sativum L.) leaves, elucidating plant responses to harsh environments. Phytohormones, 12-oxophytodienoic acid (OPDA), Jasmonic acid (JA), Jasmonyl-Isoleucine (JA-Ile), Abscisic acid (ABA), and salicylic acid (SA) increased, especially ABA that rose consistently. Indole-3-acetic acid (IAA) responded akin to ABA and SA. Cytokinins such as zeatin (ZT) and isopentenyl adenine (iP) diversified drought tolerance, ZT fell sharply, and iP remained stable. Gibberellic acid (GA3) declined, while brassinolide (BL) surged with prolonged drought. Polyamines reduced, indicating sensitivity to severe drought. Findings uncovered intricate regulatory mechanisms for plant adaptation to water scarcity. Interaction of OPDA, JA, JA-Ile, ABA, SA, IAA, ZT, iP, GA3, BL, and polyamines contributes to drought coping and physiological balance. Insights into cress leaf response and drought tolerance mechanisms contribute significantly to plant drought adaptation understanding.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"106 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139179061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}