Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale最新文献

Phytochemical characterization and evaluation of the antibacterial potential of Solenostemma oleifolium (Nectoux) Bullock & E.A. Bruce ex Maire essential oil Solenostemma oleifolium (Nectoux) Bullock & E.A. Bruce ex Maire 精油的植物化学特征和抗菌潜力评估

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale Pub Date : 2024-06-05 DOI: 10.4081/jbr.2024.12321

Daouia K. Benmaarouf, Bernard China, Baya Djallout, Soumiya Boulahlib, Hamza Aliboudhar, Diana C.G.A. Pinto, S. Zenia, M. Ben-Mahdi

{"title":"Phytochemical characterization and evaluation of the antibacterial potential of Solenostemma oleifolium (Nectoux) Bullock & E.A. Bruce ex Maire essential oil","authors":"Daouia K. Benmaarouf, Bernard China, Baya Djallout, Soumiya Boulahlib, Hamza Aliboudhar, Diana C.G.A. Pinto, S. Zenia, M. Ben-Mahdi","doi":"10.4081/jbr.2024.12321","DOIUrl":"https://doi.org/10.4081/jbr.2024.12321","url":null,"abstract":"Solenostemma oleifolium is a tropical plant growing in the Algerian desert that is traditionally used to treat several illnesses, including infection. We investigate essential oil components from leaves of Solenostemma oleifolium (EOSO) and its antibacterial activity. Using Gas Chromatography and Mass Spectrometry (GC-MS), twenty compounds were identified in EOSO, including linalool (57.10%), terpineol (12.95%), trans-geraniol (12.65%), and nerol (4.67%). Nuclear magnetic resonance (NMR) analysis allowed us to confirm linalool as the main component of EOSO. Antibacterial activity was tested by agar diffusion and microdilution methods for minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). For EOSO, the inhibition diameters ranged from 16.79 to 39.84 mm, the MIC ranged from 1.066 to 8.54 mg mL-1 and the MBC ranged from 20 to 100 mg mL-1. For linanool, the inhibition diameters ranged from 11.1 to 31.87 mm, the MIC ranged from 2.68 to 14.3 mg mL-1 and, the MBC ranged from 40 to 100 mg mL-1.EOSO and linalool exhibited significant antibacterial activity against all the tested bacteria. This study confirmed the antibacterial activity of the S. oleifolium essential oil and that linalool is the principal constituent of the EOSO.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"50 s25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141383149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Field monitoring and genetic diversity of the large copper butterfly Lycaena dispar (Lepidoptera: Lycaenidae) 大型铜蝶 Lycaena dispar（鳞翅目：鳞蝶科）的实地监测和遗传多样性

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale Pub Date : 2024-05-21 DOI: 10.4081/jbr.2024.12411

Jeong Sun Park, Seung Hyun Lee, Jee-Young Pyo, H. Jeong, Sung-Soo Kim, Iksoo Kim

{"title":"Field monitoring and genetic diversity of the large copper butterfly Lycaena dispar (Lepidoptera: Lycaenidae)","authors":"Jeong Sun Park, Seung Hyun Lee, Jee-Young Pyo, H. Jeong, Sung-Soo Kim, Iksoo Kim","doi":"10.4081/jbr.2024.12411","DOIUrl":"https://doi.org/10.4081/jbr.2024.12411","url":null,"abstract":"\u0000The large copper butterfly Lycaena dispar (Haworth, 1803) (Lepidoptera: Lycaenidae), which is found across Europe and Asia, has been categorized as a near-threatened species (NT) in South Korea from 2012 mainly due to limited distribution. The species has been infrequently observed outside its traditional range in the northwestern region, but no nationwide survey has yet been conducted. In addition, the population genetic data of L. dispar, which is informative to evaluate its vulnerability, remains unknown. In this study, we visited 36 sites spread across all South Korean provinces to verify the distributional range of the species. Mitochondrial cytochrome oxidase subunit I (COI) was also partially sequenced for 53 individuals from nine sites. We observed L. dispar at 15 sites in six provinces, including the two previously known provinces, indicating a southward range expansion. The in-field monitoring and genetic data collectively suggested that L. dispar does not have a limited distribution nor is it isolated, indicating that it should be reclassified as less vulnerable. Our study demonstrates that the combination of field and genetic data can provide a more reliable assessment of the stability of a species.\u0000","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"59 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141116974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unleashing the potential of millets: a comprehensive review of its nutritional, therapeutic, and genomic attributes 释放黍子的潜力：对其营养、治疗和基因组特性的全面审查

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale Pub Date : 2024-05-07 DOI: 10.4081/jbr.2024.12131

Biju Vadakkemukadiyil Chellappan, Rajendran Peramaiyan

{"title":"Unleashing the potential of millets: a comprehensive review of its nutritional, therapeutic, and genomic attributes","authors":"Biju Vadakkemukadiyil Chellappan, Rajendran Peramaiyan","doi":"10.4081/jbr.2024.12131","DOIUrl":"https://doi.org/10.4081/jbr.2024.12131","url":null,"abstract":"Millets are cereal grains whose farming dates back thousands of years and have been farmed and consumed by a wide variety of cultures around the world. In recent times, there has been a growing interest in millets due to their medicinal characteristics and nutritional advantages. Millets have a substantial nutritional content and can provide a wide range of beneficial health effects. These foods have a relatively low glycemic index in addition to their high levels of dietary fiber, proteins, vitamins, and minerals. In this review, the most recent information regarding the potential effects of millets on the management of diabetes, the health of the heart, antioxidant activity, anti-cancer capacities, and other therapeutic benefits is investigated. In addition, the report provides an in-depth analysis of the current genome resources that are associated with millets, as well as a summary of the key genetic discoveries that have been made. The report also emphasizes the need for further research to maximize the potential of millets through the utilization of genetic resources and breeding techniques to develop high-nutrient-rich and climate-resilient varieties.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"44 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141003265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteolytic system parameters in the brain of rats with hyperhomocysteinemia 高同型半胱氨酸血症大鼠大脑中的蛋白水解系统参数

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale Pub Date : 2024-04-24 DOI: 10.4081/jbr.2024.12232

T. Synelnyk, Nataliia Raksha, Oleksandra Kostiuk, Olga Kharchenko, Sofia Rymsha, Viktoria Korol, Anatoliy Korol, Oksana Bernyk, O. Maievskyi

{"title":"Proteolytic system parameters in the brain of rats with hyperhomocysteinemia","authors":"T. Synelnyk, Nataliia Raksha, Oleksandra Kostiuk, Olga Kharchenko, Sofia Rymsha, Viktoria Korol, Anatoliy Korol, Oksana Bernyk, O. Maievskyi","doi":"10.4081/jbr.2024.12232","DOIUrl":"https://doi.org/10.4081/jbr.2024.12232","url":null,"abstract":"Hyperhomocysteinemia (HHcy) is now being actively studied as a potential risk factor and/or biomarker for numerous pathological conditions, including brain diseases. This study aimed to analyze the proteolytic processes in the brains of rats with HHcy. Total proteolytic activity, metal-dependent, and serine proteases activities, the content of matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinases-1, cytokines, serine proteases, total protein and medium and low molecular-weight substances (MLMWS), were evaluated. HHcy was induced by DL-homocysteine thiolactone (HTL) daily intragastric administration (200 mg·kg–1 of body weight) to young and adult albino non-linear male rats for 8 weeks following rat sacrifice and brain harvesting. It was established that HHcy causes an increase in total proteolytic activity and a rise in MLMWS levels in rat brains. Serine protease activity increased to a greater extent compared to metal-dependent one, and bigger changes were observed in young rats. Rise in MMP-9 and -10 levels (in young animals), a decline in MMP-3 and -8 levels, and a decrease in the content of interleukin-1β, interferon-γ, interleukin-4 and tumor necrosis factor-α (the last two in young animals) was also detected. No significant changes were found in serine protease content. Therefore, proteolysis intensification in the brain of rats with HHcy is more likely caused by protease up-regulation through mechanisms stimulated by homocysteine, HTL, and oxidative stress, without involving pro-inflammatory signaling pathways.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"9 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140660169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between placental bacterial PCR results and histological chorioamnionitis: a prospective study on 41 placentas 胎盘细菌 PCR 结果与组织学绒毛膜羊膜炎之间的相关性：对 41 例胎盘的前瞻性研究

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale Pub Date : 2024-04-18 DOI: 10.4081/jbr.2024.11700

H. Zaidi, Laila Lahlou, Mariem Chraybi, Amina Barkat, Sabah Elamrani, Najat Lamalmi

{"title":"Correlation between placental bacterial PCR results and histological chorioamnionitis: a prospective study on 41 placentas","authors":"H. Zaidi, Laila Lahlou, Mariem Chraybi, Amina Barkat, Sabah Elamrani, Najat Lamalmi","doi":"10.4081/jbr.2024.11700","DOIUrl":"https://doi.org/10.4081/jbr.2024.11700","url":null,"abstract":"Chorioamnionitis or intra-uterine inflammation is considered the most common infection diagnosed in labor and delivery units worldwide. It is a leading cause of maternal morbidity and mortality, as well as neonatal death and sepsis. The heterogeneity of this clinical syndrome has been recently reported. The objectives of this study were to determine: 1) placental microbiology using molecular microbiological techniques; 2) diagnostic accuracy of the clinical criteria used to identify patients with placental bacterial infection; 3) relationship between placental bacterial infection and histological chorioamnionitis. This prospective cross-sectional study included 41 women diagnosed with clinical and histological chorioamnionitis. The presence of microorganisms in the placenta was determined by conducting placental analysis using a broad range of polymerase chain reactions (PCR). Bacterial placental infection (defined as the presence of the 16S gene detected in placental tissue using the molecular technique PCR) was observed in 63% (26/41) of parturients diagnosed with histological chorioamnionitis. The traditional criteria for diagnosing clinical chorioamnionitis exhibit poor diagnostic performance in accurately identifying proven intra-amniotic infection. The molecular analysis (PCR) of the placenta has suggested that acute chorioamnionitis commonly has a bacterial origin.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":" 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140689297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sudden cardiac death in the young. From gross to molecular autopsy 年轻人的心脏性猝死。从尸体解剖到分子解剖

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale Pub Date : 2024-04-18 DOI: 10.4081/jbr.2024.12184

G. Thiene, Cristina Basso, M. De Gaspari, Stefania Rizzo

引用次数: 0

In vitro biocontrol of phytopathogenic fungi isolated from the rhizosphere of multiple crops by a native Trichoderma strain 一种本地毛霉菌株对从多种作物根瘤中分离出来的植物病原真菌的体外生物控制作用

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale Pub Date : 2024-04-16 DOI: 10.4081/jbr.2024.11664

Alondra Santos Villegas, Nuria Jiménez-Juárez, Minerva Rosas Morales, Dalia Castillo-Hernández

{"title":"In vitro biocontrol of phytopathogenic fungi isolated from the rhizosphere of multiple crops by a native Trichoderma strain","authors":"Alondra Santos Villegas, Nuria Jiménez-Juárez, Minerva Rosas Morales, Dalia Castillo-Hernández","doi":"10.4081/jbr.2024.11664","DOIUrl":"https://doi.org/10.4081/jbr.2024.11664","url":null,"abstract":"Phytopathogenic fungi associated with roots and leaves can cause significant losses in crops of commercial interest due to alterations in the growth and development of the host plants. In addition, they could contaminate fruits in the postharvest stage, provoking significant economic damage. In this regard, biocontrol by antagonistic fungi such as Trichoderma sp. (Peerson, 1974) has been shown as a viable eco-friendly solution. Accordingly, in this study, four genera of native phytopathogenic fungi, namely Fusarium (Link, 1809), Botrytis (Micheli & Peersoon, 1729), Alternaria (Nees, 1817), and Colletotrichum (Corda, 1831)], as well as of native postharvest fungi, namely Rhizopus (Anton de Bary, 1886), Mucor (Saccardo, 1887), Penicillium (Friedrich, 1809), and Aspergillus (Micheli, 1728), were isolated and identified from the rhizosphere of multiple crops of an unstudied autochthonous region in Puebla, Mexico. The isolated phytopathogens were tested in dual confrontation assays against a native Trichoderma strain with presumable antagonistic activity, finding a significant growth inhibition, reported for the first time. For the phytopathogenic fungi, the highest percentage of inhibition of radial growth (PIRG) was observed in Fusarium sp., followed by Alternaria sp., and Colletotrichum sp.; for the post-harvesting fungi, the best PIRG was found in Penicillium sp. (2), followed by Aspergillus sp., Rhizopus sp., Mucor sp., and Penicillium sp. (1). ","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"6 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140695981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

96th National Congress of the Italian Society for Experimental Biology | L’Aquila, Italy, 25-28 April 2024 意大利实验生物学学会第 96 届全国代表大会，意大利拉奎拉，2024 年 4 月 25-28 日

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale Pub Date : 2024-04-12 DOI: 10.4081/jbr.2024.12560

The Editors

引用次数: 0

Comparison and evaluation of the methods for measuring hemolytic activity of Stomolophus meleagris jellyfish tentacle extract 比较和评估测量蓑鲉触手提取物溶血活性的方法

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale Pub Date : 2024-03-29 DOI: 10.4081/jbr.2024.11682

Yongfang Wang, Yiran He, Fengling Yang, Yi Wang, Zhaoyun Peng, Liang Xiao, Marina Pozzolini, Yina Zhu

{"title":"Comparison and evaluation of the methods for measuring hemolytic activity of Stomolophus meleagris jellyfish tentacle extract","authors":"Yongfang Wang, Yiran He, Fengling Yang, Yi Wang, Zhaoyun Peng, Liang Xiao, Marina Pozzolini, Yina Zhu","doi":"10.4081/jbr.2024.11682","DOIUrl":"https://doi.org/10.4081/jbr.2024.11682","url":null,"abstract":"Hemolytic activity assessment is a widely used method to evaluate the toxicity of marine organisms, including jellyfish. However, there are some challenges associated with testing hemolytic activity. In this study, four methods were employed to compare the hemolytic activity of jellyfish tentacle extract (TE). Firstly, a microplate reader was used to compare the mouse hemoglobin solution at three different wavelengths (415 nm, 541 nm, and 576 nm), and the most sensitive wavelength was selected for further experiments. Secondly, photomicrograph counting was used to determine the number of complete red blood cells in the field of view. Thirdly, a microplate reader was used to test hemolytic activity in a 96-well plate at 415 nm. Fourthly, a Bicinchoninic Acid (BCA) kit was used to test the concentration of hemoglobin in the solution. Finally, a UV-Vis Spectrophotometer was used to test hemolytic activity at 415 nm. Among the three wavelengths tested, the absorption value was most sensitive at 415 nm. The photomicrograph counting method was able to reflect changes in the shape of Red Blood Cells (RBCs). The microplate reader method may exhibit deviations when the solution concentration is high, while external factors could influence the BCA kit when the toxin concentration in the experimental group is low. The spectrophotometer method was found to be relatively accurate and sensitive to changes. When optimizing the method, it is important to consider the applicability of the Beer-Lambert law and the concentration of solutions.","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":"42 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140368507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of selenium in autoimmune thyroiditis 硒在自身免疫性甲状腺炎中的作用

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale Pub Date : 2024-03-21 DOI: 10.4081/jbr.2024.12050

Marco Giammanco, Vincenza M.E. Bonfiglio, M. Giammanco, Francesco Carini

{"title":"The role of selenium in autoimmune thyroiditis","authors":"Marco Giammanco, Vincenza M.E. Bonfiglio, M. Giammanco, Francesco Carini","doi":"10.4081/jbr.2024.12050","DOIUrl":"https://doi.org/10.4081/jbr.2024.12050","url":null,"abstract":"\u0000Selenium (Se) is an essential trace element of fundamental importance for human health. Se is incorporated into selenoproteins (SPs) which are endowed with pleiotropic effects including antioxidant and anti-inflammatory effects and active production of thyroid hormones. It has also been suggested that Se plays a crucial role in the pathogenesis of various human diseases. The therapeutic effects of supplementation with Se have already been described in various thyroid diseases. However, there are still conflicting results regarding the optimal dose of Se to administer and the duration of treatment, efficacy, and safety. The highly beneficial effects of supplementation with Se have been observed in subjects with thyroid disease in the hyperthyroid phase. In line with these observations, clinical studies have shown that in patients with Basedow's disease (BD) and autoimmune thyroiditis (AT), treatment with a combination of antithyroid drugs and Se restores the euthyroid state faster than administration of antithyroid drugs alone. However, the efficacy of this therapeutic approach remains to be better evaluated. \u0000","PeriodicalId":506664,"journal":{"name":"Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale","volume":" 61","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140221141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0