Development Growth & Differentiation最新文献_第8页

Crosstalk between the mTOR and Hippo pathways mTOR和Hippo通路之间的串扰

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-20 DOI: 10.1111/dgd.12867

Daichi Honda, Misako Okumura, Takahiro Chihara

引用次数: 3

Ex vivo expansion of primary cells from limb tissue of Pleurodeles waltl 侧耳侧壁肢组织原代细胞的体外扩增

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-20 DOI: 10.1111/dgd.12866

Md. Mahmudul Hasan, Reiko Sekiya, Tao-Sheng Li

{"title":"Ex vivo expansion of primary cells from limb tissue of Pleurodeles waltl","authors":"Md. Mahmudul Hasan, Reiko Sekiya, Tao-Sheng Li","doi":"10.1111/dgd.12866","DOIUrl":"10.1111/dgd.12866","url":null,"abstract":"Pleurodeles waltl is coming to light as a model animal, especially in regeneration studies, but deep studies on the molecular mechanisms have been limited due to the absence of primary tissue cells for wide usage. Therefore, we aimed to grow primary cells from limb tissue of P. waltl for in vitro experiments. Limb tissues were cut into small pieces and seeded as “explants” on culture dishes coated with fibronectin and gelatin. Compared to the control without coating, both fibronectin and gelatin supported quicker outgrowth of cells from explants and faster cell adhesion, and fibronectin showed significantly better performance than gelatin. Interestingly, the doubling time of cells on fibronectin- and gelatin-coated surfaces was almost the same (42.39 ± 2.79 h vs. 42.91 ± 3.69 h) and was not significantly different from that on non-coated plates (49.64 ± 3.63 h). The cryopreserved cells were successfully recovered and showed a multiplication capacity that was similar to that of fresh cells. Senescent cells were barely detected even after long-term sub-culture (>15 passages). Moreover, enhanced fluorescence of MitoSOX™ Red in cells under H2O2 exposure confirmed the respondence to chemical stimuli. Collectively, our results show that we are able to grow enough good-quality cells from P. waltl limb tissue for in vitro experiments, and fibronectin coating provides the best biocompatible environment for cell outgrowth and attachment.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"65 5","pages":"255-265"},"PeriodicalIF":2.5,"publicationDate":"2023-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9649283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mathematical modeling for developmental processes 发展过程的数学建模

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-16 DOI: 10.1111/dgd.12856

Yoh Iwasa

{"title":"Mathematical modeling for developmental processes","authors":"Yoh Iwasa","doi":"10.1111/dgd.12856","DOIUrl":"10.1111/dgd.12856","url":null,"abstract":"We review several mathematical models and concepts in developmental biology that have been established over the last decade. (1) Feedback vertex set: Ascidian embryos contain cells of seven types, and cell fate is controlled by ~100 interacting genes. The “feedback vertex set” of the directed graph of the gene regulatory network consists of a small number of genes. By experimentally manipulating them, we can differentiate cells into any cell type. (2) Tissue deformation: Describing morphological changes in tissues and relating them to gene expression and other cellular processes is key in understanding morphogenesis. Expansion and anisotropy of the tissue are described by a “deformation tensor” at each location. A study on chick limb bud formation revealed that both the volume growth rate and anisotropy in deformation differed significantly between locations and stages. (3) Mechanobiology: Forces operating on each cell may alter cell shape and gene expression, which may subsequently exert forces on their surroundings. Measurements of force, tissue shape, and gene expression help us understand autonomous tissue deformation. (4) Adaptive design of development: An optimal growth schedule in fluctuating environments explains the growth response to starvation in Drosophila larvae. Adaptive placement of morphogen sources makes development robust to noises.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"65 5","pages":"272-281"},"PeriodicalIF":2.5,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12856","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9642773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A geometrical perspective on development 发展的几何视角

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-16 DOI: 10.1111/dgd.12855

Archishman Raju, Eric D. Siggia

引用次数: 3

Step-by-step protocol for alternative injury models in newt cardiac regeneration 蝾螈心脏再生替代损伤模型的分步方案

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-08 DOI: 10.1111/dgd.12854

Hiromi Ikuta, Hitoshi Uemasu, Mitsuki Kyakuno, Yukio Satoh, Noriyuki Namba, Takashi Takeuchi, Toshinori Hayashi

{"title":"Step-by-step protocol for alternative injury models in newt cardiac regeneration","authors":"Hiromi Ikuta, Hitoshi Uemasu, Mitsuki Kyakuno, Yukio Satoh, Noriyuki Namba, Takashi Takeuchi, Toshinori Hayashi","doi":"10.1111/dgd.12854","DOIUrl":"10.1111/dgd.12854","url":null,"abstract":"Although the heart is one of the most important organs for animal survival, its regenerative capacity varies among animal species. Notably, adult mammals cannot regenerate their hearts after damage such as acute myocardial infarction. In contrast, some vertebrate animals can regenerate the heart throughout their lives. Cross-species comparative studies are important to understand the full picture of cardiac regeneration in vertebrates. Among the animal species able to regenerate the heart, some urodele amphibians, such as newts, possess a remarkable capacity for this process. Standardized methods of inducing cardiac regeneration in the newt are needed as a platform for studies comparing newts and other animal models. The procedures presented here describe amputation and cryo-injury techniques for the induction of cardiac regeneration in Pleurodeles waltl, an emerging model newt species. Both procedures consist of simplified steps that require no special equipment. We additionally show some examples of the regenerative process obtained using these procedures. This protocol has been developed for P. waltl. However, these methods are also expected to be applicable to other newt and salamander species, facilitating comparative research with other model animals.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"65 5","pages":"266-271"},"PeriodicalIF":2.5,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10006400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Appetizer on soft matter physics concepts in mechanobiology 力学生物学中软物质物理概念的开胃菜

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-01 DOI: 10.1111/dgd.12853

Yuting Lou

引用次数: 0

Molecular functions of the double-sided and inverted ubiquitin-interacting motif found in Xenopus tropicalis cryptochrome 6 热带爪蟾隐花6双面和反向泛素相互作用基序的分子功能

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-05-01 DOI: 10.1111/dgd.12852

Keiko Okano, Hiroaki Otsuka, Marika Nakagawa, Toshiyuki Okano

{"title":"Molecular functions of the double-sided and inverted ubiquitin-interacting motif found in Xenopus tropicalis cryptochrome 6","authors":"Keiko Okano, Hiroaki Otsuka, Marika Nakagawa, Toshiyuki Okano","doi":"10.1111/dgd.12852","DOIUrl":"10.1111/dgd.12852","url":null,"abstract":"Cryptochromes (CRYs) are multifunctional molecules that act as a circadian clock oscillating factor, a blue-light sensor, and a light-driven magnetoreceptor. Cry genes are classified into several groups based on the evolutionary relationships. Cryptochrome 6 gene (Cry6) is present in invertebrates and lower vertebrates such as amphibians and fishes. Here we identified a Cry6 ortholog in Xenopus tropicalis (XtCry6). XtCRY6 retains a conserved long N-terminal extension (termed CRY N-terminal extension; CNE) that is not found in any CRY in the other groups. A structural prediction suggested that CNE contained unique structures; a tetrahelical fold structure topologically related to KaiA/RbsU domain, overlapping nuclear- and nucleolar-localizing signals (NLS/NoLS), and a novel motif (termed DI-UIM) overlapping a double-sided ubiquitin-interacting motif (DUIM) and an inverted ubiquitin-interacting motif (IUIM). Potential activities of the NLS/NoLS and DI-UIM were examined to infer the molecular function of XtCRY6. GFP-NLS/NoLS fusion protein exogenously expressed in HEK293 cells was mostly observed in the nucleolus, while GFP-XtCRY6 was observed in the cytoplasm. A glutathione S-transferase (GST) pull-down assay suggested that the DI-UIM physically interacts with polyubiquitin. Consistently, protein docking simulations implied that XtCRY6 DI-UIM binds two ubiquitin molecules in a relationship of a twofold rotational symmetry with the symmetry axis parallel or perpendicular to the DI-UIM helix. These results strongly suggested that XtCRY6 does not function as a circadian transcriptional repressor and that it might have another function such as photoreceptive molecule regulating light-dependent protein degradation or gene expression through a CNE-mediated interaction with ubiquitinated proteins in the cytoplasm and/or nucleolus.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"65 4","pages":"203-214"},"PeriodicalIF":2.5,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/dgd.12852","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9884241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Live imaging observation of elevation of the anterior palatal shelf in mouse embryos 小鼠胚胎前腭架抬高的实时成像观察

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-04-25 DOI: 10.1111/dgd.12851

Arata Nagasaka, Koji Sakiyama, Yasuhiko Bando, Go Onozawa, Osamu Amano

{"title":"Live imaging observation of elevation of the anterior palatal shelf in mouse embryos","authors":"Arata Nagasaka, Koji Sakiyama, Yasuhiko Bando, Go Onozawa, Osamu Amano","doi":"10.1111/dgd.12851","DOIUrl":"10.1111/dgd.12851","url":null,"abstract":"The mammalian secondary palate develops through complex processes including palatal shelf growth, elevation, and fusion. Palatal shelf elevation is a process accompanied by large-scale morphological changes over a short period. The elevation pattern changes along the anterior–posterior axis; the anterior region elevates by the “flip-up” model, and the middle and posterior regions reorient through the “flow” model. However, the mechanisms of both models are unclear because of the rapid progression of the elevation in utero. To observe palatal elevation in real time in detail, we aimed to establish a live imaging method using explants of the anterior region of the palatal shelf in mouse embryos before the beginning of elevation. Changes in the degree of shelf orientation were measured, which showed that the palatal shelf continuously changed shape toward the lingual side. The changes in the angle between the lingual and buccal bases of the palatal shelf were different; the morphological change at the lingual side resulted in a more acute angle, and the change at the buccal side resulted in a more obtuse angle. The morphological changes of the lingual and buccal sides occurred nearly simultaneously, suggesting that the anterior region of the palatal shelf in vitro elevated according to the “flip-up” model. This live imaging method enables the continuous observation of palatal shelf elevation and provides new insights into palatogenesis.","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":"65 4","pages":"224-229"},"PeriodicalIF":2.5,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9528718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in developmental biology using next-generation sequencing (NGS): Workshop for next-generation researchers 下一代测序技术在发育生物学中的应用进展:下一代研究人员研讨会

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-03-24 DOI: 10.1111/dgd.12850

Makoto Kashima

引用次数: 0

Meeting report: The Third Franco-Japanese Developmental Biology Meeting 会议报告:第三届法日发育生物学会议

IF 2.5 4区生物学

Development Growth & Differentiation Pub Date : 2023-03-15 DOI: 10.1111/dgd.12849

Yu Ieda, Naoto Ueno

引用次数: 0