International Journal of Biological Markers最新文献

{"title":"The association of hepatitis C virus infection and thyroid disease: A systematic review and meta-analysis.","authors":"Hongpeng Wang,&nbsp;Yixiu Liu,&nbsp;Yanguang Zhao","doi":"10.1177/17246008211056959","DOIUrl":"https://doi.org/10.1177/17246008211056959","url":null,"abstract":"<p><p>Previous studies have reported that hepatitis C virus (HCV) infection may increase the risk of thyroid disease (TD) even thyroid cancer (TC), but quantitative assessments of risk were rare and the results were not consistent. The purpose of this study was to evaluate the impact of HCV infection on TD and TC, and provide clues to explore the relationship between HCV infection and TD and TC. The literature retrieval was performed up to August 20th, 2021 in the database of PubMed, Cochrane library, Web of Science, China National Knowledge Infrastructure and Wang Fang. The risk of HCV for TD or TC was expressed with odds ratio (OR) and 95% confidence intervals (CI). Subgroup analysis was used to explore the source of heterogeneity. Six articles (three studies published as article and three studies published as abstract) were included in this meta-analysis, with a total of 5398 controls and 1925 cases of hepatitis C. The results of meta-analysis found that HCV infection were significantly associated with an increased risk of TD (sum OR = 1.80, 95% CI = 1.54-2.10, <i>P</i> <  0.001, <i>I</i>² = 74.3%) and TC (sum OR = 16.36, 95% CI = 4.65-57.62, <i>P</i> < 0.001, <i>I</i>² = 0%). HCV infection may increase the risk of TD and TC. More work is needed in the future to establish a causal role, however an awareness of the possibility of increased risk of TD and TC may lead to earlier diagnosis and better outcomes in patients with hepatitis C.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":"36 4","pages":"3-9"},"PeriodicalIF":2.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39773417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circ_0039411 promotes papillary thyroid carcinoma development through mediating the miR-423-5p/SOX4 signaling.","authors":"Xiaohui Wen,&nbsp;Jingyan Du,&nbsp;Xun Wang","doi":"10.1177/17246008211043128","DOIUrl":"https://doi.org/10.1177/17246008211043128","url":null,"abstract":"<p><strong>Background: </strong>Papillary thyroid carcinoma is the most frequent histological subtype of thyroid cancer with a high incidence. We aimed to explore the function of circular RNA_0039411 (circ_0039411) and its associated mechanism in papillary thyroid carcinoma progression.</p><p><strong>Methods: </strong>Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot assay were conducted to determine the expression of RNA and protein, respectively. The colony formation ability, migration, invasion, and apoptosis were analyzed by colony formation assay, transwell migration assay, transwell invasion assay, and flow cytometry. Cell glycolytic metabolism was analyzed using fluorescence-based glucose assay kit and fluorescence-based lactate assay kit. Dual-luciferase reporter assay and RNA-Pull-Down Assay were performed to validate the binding between microRNA-423-5p (miR-423-5p) and circ_0039411 or SRY-box transcription factor 4 (SOX4). The xenograft tumor model was used to assess the role of circ_0039411 in the tumor growth in vivo.</p><p><strong>Results: </strong>Circ_0039411 was highly expressed in papillary thyroid carcinoma tissues and cell lines compared with adjacent normal tissues and NTHY-ORI3.1 cells. Circ_0039411 interference suppressed the colony formation ability, migration, invasion, and glycolysis but promoted the apoptosis of papillary thyroid carcinoma cells. MiR-423-5p was a target of circ_0039411 in papillary thyroid carcinoma cells. Circ_0039411 knockdown-mediated effects in papillary thyroid carcinoma cells were largely overturned by the silence of miR-423-5p. MiR-423-5p bound to the 3' untranslated region (3'UTR) of SOX4. SOX4 overexpression largely reversed circ_0039411 silencing-mediated effects in papillary thyroid carcinoma cells. Circ_0039411 positively regulated SOX4 expression by sponging miR-423-5p in papillary thyroid carcinoma cells. Circ_0039411 silencing notably suppressed the growth of xenograft tumors in vivo.</p><p><strong>Conclusion: </strong>Circ_0039411 promoted the malignant behaviors of papillary thyroid carcinoma cells partly depending on the regulation of the miR-423-5p/SOX4 axis.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":"36 4","pages":"10-20"},"PeriodicalIF":2.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39698095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BAX gene (-248 G > A) polymorphism in a sample of patients diagnosed with thyroid cancer in the Federal District, Brazil.","authors":"Ligia C A Cardoso-Duarte,&nbsp;Caroline F Fratelli,&nbsp;Alexandre S R Pereira,&nbsp;Jéssica Nayane Gomes de Souza,&nbsp;Renata de Souza Freitas,&nbsp;Rafael Martins de Morais,&nbsp;Alaor Barra Sobrinho,&nbsp;Calliandra M Sousa Silva,&nbsp;Jamila Reis de Oliveira,&nbsp;Diêgo Madureira de Oliveira,&nbsp;Izabel Cristina R Silva","doi":"10.1177/17246008211057576","DOIUrl":"https://doi.org/10.1177/17246008211057576","url":null,"abstract":"<p><strong>Introduction: </strong>Papillary thyroid cancer corresponds to approximately 1% of all carcinomas; nevertheless, it is the most prevalent endocrine neoplasm in the world. Studies reveal that the <i>BAX</i> (-248 G > A) polymorphism may be associated with negative regulation of <i>BAX</i> gene transcription activity, causing a decrease in its protein expression.</p><p><strong>Objective: </strong>The present study aimed to describe the genotype and allele frequencies of <i>BAX</i> single nucleotide polymorphisms (-248 G > A) (rs4645878) in the research patients, and to associate its presence with susceptibility to papillary thyroid cancer.</p><p><strong>Methods: </strong>This case-control study was conducted with 30 patients with papillary thyroid cancer. For the evaluation of genetic polymorphisms, the polymerase chain reaction-restriction fragment length polymorphism technique was employed. Allele and genotype frequencies were estimated using the SPSS program, and significant associations were considered when p < 0.05.</p><p><strong>Results: </strong>There was a significant genotypic difference between papillary thyroid cancer and the control group (p = 0.042). The GG genotype provided a protective factor for papillary thyroid cancer (p = 0.012, odds ratio (OR) = 0.313; confidence interval (CI) = 0.123-0.794). Likewise the G allele was a protective factor for papillary thyroid cancer (p = 0.009; OR = 0.360; CI = 0.163-0.793). The <i>BAX</i> gene polymorphism (-248 G > A) was associated with papillary thyroid cancer.</p><p><strong>Conclusion: </strong><i>BAX</i> (-248 G > A) GG genotype carriers, or at least one mutated allele, was associated with papillary thyroid cancer in the Brazilian population studied, and the G allele presence is considered a protective factor against papillary thyroid cancer occurrence.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":"36 4","pages":"21-26"},"PeriodicalIF":2.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39749718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SOX2 as a prognostic marker and a potential molecular target in cervical cancer: A meta-analysis.","authors":"Dandan Yuan,&nbsp;Jian Wang,&nbsp;Mingyu Yan,&nbsp;Yaohui Xu","doi":"10.1177/17246008211042899","DOIUrl":"https://doi.org/10.1177/17246008211042899","url":null,"abstract":"<p><strong>Background: </strong>Sex determining region Y-box 2 (SOX2) has been reported as a potential therapeutic target for cancer. However, the role of SOX2 in cervical cancer remains largely undetermined. This study was performed to evaluate the correlation of SOX2 with clinical characteristics and prognosis in cervical cancer.</p><p><strong>Methods: </strong>Multiple databases were systematically searched for eligible publications. The combined odds ratios (ORs) or hazard ratios (HRs) with the corresponding 95% confidence intervals (CIs) were used to assess the effect sizes.</p><p><strong>Results: </strong>A total of 17 studies with 1906 participants were identified. SOX2 expression was higher in cervical cancer than in the normal control group (OR = 10.83, 95% CI = 6.64-17.67, <i>P</i> < 0.001), while no significant difference was observed between cervical cancer and cervical intraepithelial neoplasia. SOX2 expression was not associated with age, tumor stage, and lymph node metastasis, but was correlated with tumor grade (grade 2-3 vs. grade 1: OR = 4.59, 95% CI = 2.76-7.62, <i>P</i> < 0.001) and tumor size (≥4 cm vs. ≤4 cm: OR = 1.66, 95% CI = 1.05-2.60, <i>P</i> = 0.028). Based on multivariate Cox analysis, SOX2 expression was not correlated with overall survival, but was closely associated with poor recurrence-free survival (HR = 5.83, 95% CI = 1.35-25.16, <i>P</i> = 0.018) and progress-free survival HR = 2.29, 95% CI = 1.01-5.19, <i>P</i> = 0.046).</p><p><strong>Conclusion: </strong>SOX2 may serve as a novel prognostic factor and a promising molecular target for cervical cancer.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":"36 4","pages":"45-53"},"PeriodicalIF":2.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39578067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The diagnostic value of the combination of carcinoembryonic antigen, squamous cell carcinoma-related antigen, CYFRA 21-1, neuron-specific enolase, tissue polypeptide antigen, and progastrin-releasing peptide in small cell lung cancer discrimination.","authors":"Zhimao Chen,&nbsp;Xiangzheng Liu,&nbsp;Xueqian Shang,&nbsp;Kang Qi,&nbsp;Shijie Zhang","doi":"10.1177/17246008211049446","DOIUrl":"https://doi.org/10.1177/17246008211049446","url":null,"abstract":"<p><strong>Background: </strong>The diagnostic value of six tumor markers was investigated and the appropriate combinations of those tumor markers to discriminate small cell lung cancer was explored.</p><p><strong>Methods: </strong>Patients suspected with lung cancer (1938) were retrospectively analyzed. Candidate tumor markers from carcinoembryonic antigen (CEA), squamous cell carcinoma-related antigen (SCC), cytokeratin 19 fragment 21-1 (CYFRA 21-1), neuron-specific enolase (NSE), tissue polypeptide antigen (TPA), and progastrin releasing peptide (ProGRP) were selected to construct a logistic regression model. The receiver operating characteristic curve was used for evaluating the diagnostic value of the tumor markers and the predictive model.</p><p><strong>Results: </strong>ProGRP had the highest positive rate (72.3%) in diagnosed small cell lung cancer, followed by neuron-specific enolase (68.3%), CYFRA21-1 (50.5%), carcinoembryonic antigen (45.5%), tissue polypeptide antigen (30.7%), and squamous cell carcinoma-related antigen (5.9%). The predictive model for small cell lung cancer discrimination was established, which yielded the highest area under the curve (0.888; 95% confidence interval: 0.846-0.929), with a sensitivity of 71.3%, a specificity of 95.0%, a positive predictive value of 49.0%, and a negative predictive value of 98.0%.</p><p><strong>Conclusions: </strong>Combining tumor markers can improve the efficacy for small cell lung cancer discrimination. A predictive model has been established in small cell lung cancer differential diagnosis with preferable efficacy.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":"36 4","pages":"36-44"},"PeriodicalIF":2.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39565118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relevance analysis of GSTP1 rs1695 and lung cancer in the Chinese Han population.","authors":"Jiang Xiao,&nbsp;Yulu Wang,&nbsp;Zhimin Wang,&nbsp;Yao Zhang,&nbsp;Yutao Li,&nbsp;Chang Xu,&nbsp;Man Xiao,&nbsp;Haijian Wang,&nbsp;Shicheng Guo,&nbsp;Li Jin,&nbsp;Jiucun Wang,&nbsp;Yang Bao,&nbsp;Yan Shang,&nbsp;Junjie Wu","doi":"10.1177/17246008211039236","DOIUrl":"https://doi.org/10.1177/17246008211039236","url":null,"abstract":"<p><strong>Background: </strong>This study explored the relevance between rs1695 and susceptibility to the lung cancer in the Chinese Han population. Stratification analysis was conducted on the basis of age, gender, smoking status, tumor-related family history, and pathological type to observe relations between rs1695 and susceptibility to lung cancer in the subgroups.</p><p><strong>Methods: </strong>A case-control study was performed with 974 lung cancer patients who were pathologically diagnosed and 1005 healthy cases based on physical examination to analyze the association between rs1695 and the risk of lung cancer.</p><p><strong>Results: </strong>The frequencies of the AA, GA, and GG genotypes of rs1695 were 68.4%, 28.7%, and 2.9% in cases and 64.8%, 30.8%, and 4.2% in controls, respectively. After adjustment for age, gender, smoking status, and family history, it appears that the rs1695 G allele decreases the risk of lung cancer (OR = 0.811, 95% CI 0.684-0.961, <i>P</i> = 0.016). Moreover, compared with the AA genotype, the GA + GG genotype decreased lung cancer susceptibility (OR = 0.808, 95% CI 0.663-0.985, <i>P</i> = 0.035) and the GG genotype (OR = 0.591, 95% CI 0.347-0.988, <i>P</i> = 0.048). In a stratified analysis, the risk of lung cancer in the G allele carriers decreased among the males, patients without a tumor-related family history, and patients with lung adenocarcinoma, especially in smokers.</p><p><strong>Conclusion: </strong>The polymorphism of locus rs1695 is related to the risk of lung cancer and is expected to be a target for the prediction of lung cancer.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":"36 3","pages":"48-54"},"PeriodicalIF":2.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39477823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circ_0006174 promotes colorectal cancer progression by sponging microRNA-142-3p and regulating X-linked inhibitor of apoptosis expression.","authors":"Bo Huang,&nbsp;Dejun Cui,&nbsp;Ying Ren,&nbsp;Xun Zhao,&nbsp;Fei Li,&nbsp;Wenqiang Yuan","doi":"10.1177/17246008211034178","DOIUrl":"https://doi.org/10.1177/17246008211034178","url":null,"abstract":"<p><strong>Background: </strong>Circular RNAs (circRNAs) are crucial in the regulation of gene expression and biological processes. However, in colorectal cancer, the expression characteristics and biological function of circRNA_0006174 (circ_0006174) is not fully understood. This work is aimed to investigate the biological function of circ_0006174 in colorectal cancer and its molecular mechanism.</p><p><strong>Methods: </strong>Circ_0006174, microRNA-142-3p and X-linked inhibitor of apoptosis expression levels were detected in colorectal cancer tissues and cells using quantitative real-time polymerase chain reaction analysis or Western blot. The effects of circ_0006174 on colorectal cancer cell proliferation, apoptosis, migration and invasion were detected using the cell counting kit-8 method, bromodeoxyuridine experiments, flow cytometry analysis and Transwell experiments. The targeting relationship among circ_0006174, microRNA-142-3p and X-linked inhibitor of apoptosis was analysed by bioinformatics prediction, dual-luciferase reporter experiment and RNA immunoprecipitation experiment.</p><p><strong>Results: </strong>Circ_0006174 was up-regulated in colorectal cancer tissues as well as in cell lines, and its high expression was remarkably associated with enlarged tumour volume and advanced tumour, node, metastasis stage of the patients. Circ_0006174 overexpression enhanced colorectal cancer cell proliferation, migration and invasion, and inhibited colorectal cancer cell apoptosis; while knocking down circ_0006174 caused the opposite effects. Circ_0006174 directly targeted and negatively regulated microRNA-142-3p expression, and X-linked inhibitor of apoptosis, a target gene of microRNA-142-3p, could be indirectly and positively modulated by circ_0006174.</p><p><strong>Conclusion: </strong>Circ_0006174 facilitates colorectal cancer cell proliferation, migration and invasion, and represses colorectal cancer cell apoptosis by regulating microRNA-142-3p/X-linked inhibitor of apoptosis axis.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":"36 3","pages":"3-13"},"PeriodicalIF":2.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39325190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum epithelial membrane protein 1 serves as a feasible biomarker in extrahepatic cholangiocarcinoma.","authors":"Xiang Li,&nbsp;Lang Yan,&nbsp;Hao Xue","doi":"10.1177/17246008211035142","DOIUrl":"https://doi.org/10.1177/17246008211035142","url":null,"abstract":"<p><strong>Background: </strong>Extrahepatic cholangiocarcinoma is a malignancy that originates from bile duct epithelium with an unfavorable prognosis. Epithelial membrane protein 1 was first discovered in 1995, functioning as an oncogene or anti-tumor gene in various cancers. However, the clinical role of epithelial membrane protein 1 extrahepatic cholangiocarcinoma remained unclear.</p><p><strong>Methods: </strong>Differentially expressed genes were identified using Gene Ontology and the Kyoto Encyclopedia and Genomes pathway analysis. Out of 183 extrahepatic cholangiocarcinoma patients and 61 healthy controls, the expression level of epithelial membrane protein 1 was detected and compared using reverse transcription-quantitative polymerase chain reaction analysis and western blot assay. Meanwhile, the diagnosis and prognosis of EMP1 in ECCA were measured by receiver operating characteristic and Kaplan-Meier analysis. Finally, the relationship between epithelial membrane protein 1 expression and clinicopathological indexes were compared to further verify the clinical role of epithelial membrane protein 1 in extrahepatic cholangiocarcinoma.</p><p><strong>Results: </strong>After analyzing data from GSE76297, GSE89749, and GSE26566GO, we found 1554 down-regulated and 1065 up-regulated genes. Through Gene Ontology and Kyoto Encyclopedia and Genomes analysis, extracellular matrix organization, extracellular structure organization, cholesterol metabolism, interleukin-17 signaling pathway, and vitamin digestion and absorption were significantly enriched and involved in targeted differentially expresses genes. Epithelial membrane protein 1 messenger ribonucleic acid was notably decreased in serum samples from extrahepatic cholangiocarcinoma patients, compared with that in healthy controls. Receiver operating characteristic analysis revealed that the area under the curve of epithelial membrane protein 1 messenger ribonucleic acid for the diagnosis of extrahepatic cholangiocarcinoma was 0.9281 (95% CI = 0.8967-0.9595). Moreover, the correlation analysis presented that epithelial membrane protein 1 expression was negatively correlated with lymph node metastasis, tumour node metastasis stage, cancer antigen 19-9 level, and carcinoembryonic antigen level.</p><p><strong>Conclusion: </strong>Aberrant expression of epithelial membrane protein 1 contributed to distinguishing extrahepatic cholangiocarcinoma patients and healthy controls, and a low expression level of epithelial membrane protein 1 indicated an unfavorable prognosis. Hence, epithelial membrane protein 1 was a feasible and credible biomarker for extrahepatic cholangiocarcinoma diagnosis and prognosis, with high accuracy, sensitivity, and specificity.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":"36 3","pages":"33-39"},"PeriodicalIF":2.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39454260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TMPRSS4 overexpression promotes the metastasis of colorectal cancer and predicts poor prognosis of stage III-IV colorectal cancer.","authors":"Xue-Feng Zhao,&nbsp;Yu-Shen Yang,&nbsp;Da-Zhi Gao,&nbsp;Young Kyu Park","doi":"10.1177/17246008211046368","DOIUrl":"https://doi.org/10.1177/17246008211046368","url":null,"abstract":"<p><strong>Purpose: </strong>To study in detail the expression pattern and prognostic significance of TMPRSS4 in colorectal cancer.</p><p><strong>Methods: </strong>The expression of TMPRSS4 protein was determined using Western blot in the colorectal cancer tissues and normal tissues. Immunohistochemistry was used to detect the TMPRSS4 expression in colorectal cancer tissues, and the clinicopathologic characteristics and prognostic significance were analyzed.</p><p><strong>Results: </strong>TMPRSS4 overexpression was associated with tumor budding, lymphovascular invasion, perineural invasion, cancerous emboli, infiltration depth, lymph node metastasis, distant metastasis, and tumor node metastasis stage (<i>P</i> < 0.05 for all). Interestingly, TMPRSS4 expression in the tumor budding, tumor emboli, lymph node, and liver metastatic tumor samples was higher than in the paired primary tumors. In contrast, TMPRSS4 overexpression is inversely correlated with both the overall survival and the disease-free survival of the patients with colorectal cancer (<i>P</i> < 0.05 for both). Also, we found that TMPRSS4 is only of significance in predicting the prognosis of stage III and IV colorectal cancer, not stage I and II.</p><p><strong>Conclusions: </strong>TMPRSS4 was shown to be involved in the whole process of metastasis from tumor budding to lymph node and/or distant metastasis in colorectal cancer and predicted the unfavorable prognosis of stage III-IV, indicating that it is a novel target for the precise treatment of colorectal cancer with lymph node or distant organ metastasis.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":"36 3","pages":"23-32"},"PeriodicalIF":2.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39475990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical characteristics and prognostic value of the <i>KRAS</i> mutation in Chinese colorectal cancer patients.","authors":"Ye Yuan,&nbsp;Yingting Liu,&nbsp;Ye Wu,&nbsp;Junling Zhang,&nbsp;Chunti Shen,&nbsp;Feng Zhang,&nbsp;Changping Wu,&nbsp;Wenwei Hu","doi":"10.1177/17246008211017152","DOIUrl":"https://doi.org/10.1177/17246008211017152","url":null,"abstract":"<p><strong>Background: </strong>The <i>KRAS</i> mutations are high-frequency somatic mutations found in colorectal cancer patients from Western and Asian countries however, with the exception of exon 2 of <i>KRAS</i>, other prevalence and prognostic values have not been adequately assessed in Asian patients. The aim of this study was to determine the mutation frequencies of whole exon mutations of <i>KRAS</i> in Chinese colorectal cancer patients and to investigate their impact on prognosis.</p><p><strong>Methods: </strong>A total of 7189 tumor tissue samples (iCohort) were subjected to next-generation sequencing for detection of <i>KRAS</i> mutations. All pathologic or likely pathologic mutations of <i>KRAS</i> were considered. In addition, clinical features and prognostic dates were collected from 145 patients at The Third Affiliated Hospital of Soochow University, China (sCohort) and used droplet digital™ polymerase chain reaction to detect <i>KRAS</i> mutations.</p><p><strong>Results: </strong>In the iCohort, 2706 patients (37.6%) were confirmed harboring <i>KRAS</i> mutations. The most frequent of these mutations were <i>G12D</i> (32.19%), <i>G12V</i> (17.96%), and <i>G13D</i> (17.59%). In the sCohort, 51 colorectal cancer patients (35.17%) had <i>KRAS</i> mutations, among which <i>KRAS G12D</i> (64.71%), <i>G13D</i> (29.41%), and <i>G14D</i> (3.92%) were high-frequency. The <i>KRAS</i> mutations were associated with shorter median overall survival than wild-type tumors (69 vs. 55 months; HR 1.80; 95% Cl 1.22, 2.64; <i>P</i>=0.0003). In the Cox multivariate analysis, age (HR 1.562; 95% Cl 1.10, 2.22; <i>P</i>=0.013), tumor differentiation (HR 0.417; 95% Cl 0.19, 0.90; <i>P</i>=0.026), and <i>KRAS</i> mutation (HR 1.897; 95% Cl 0.19, 0.90; <i>P</i>=0.001) remained independent predictors of shorter overall survival. Among the common <i>KRAS</i> mutations, <i>G12D</i> was significantly associated with shorter overall survival (HR 2.17; 95% Cl 1.31, 3.58; <i>P</i> < 0.0001) compared with <i>KRAS</i> wild-type patients.</p><p><strong>Conclusions: </strong>Our findings indicate that KRAS genes are frequently mutated, and over 30% harbored the <i>KRAS G12D</i> mutation subtype. We found that the <i>KRAS G12D</i> mutation is associated with inferior survival and is a biomarker of poor prognosis in Chinese patients. Our data emphasize the importance of molecular features in colorectal cancer patients, which could potentially be improved by <i>G12D</i>-specific related inhibitors.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":"36 2","pages":"33-39"},"PeriodicalIF":2.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/17246008211017152","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39039833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}