International Journal of Biological Markers最新文献

{"title":"Corrigendum to \"The diagnostic value of midkine as a novel serum biomarker in alpha-fetoprotein-negative hepatocellular carcinoma\".","authors":"","doi":"10.1177/03936155251380644","DOIUrl":"https://doi.org/10.1177/03936155251380644","url":null,"abstract":"","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":" ","pages":"3936155251380644"},"PeriodicalIF":2.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145253447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The autophagy-related gene <i>PEA15</i> is a potential prognostic biomarker for early-stage endometrial carcinoma.","authors":"Qiao Qin, Guoqing Li, Jing Cai, Sha Hu, Liqiong Cai","doi":"10.1177/03936155251383945","DOIUrl":"https://doi.org/10.1177/03936155251383945","url":null,"abstract":"<p><p>BackgroundThe Cancer Genome Atlas (TCGA) molecular classification has advanced risk stratification for endometrial carcinoma but has demonstrated comparable survival outcomes between the microsatellite instability (MSI) and copy-number low (CN-L) subtypes. In this study, we aimed to identify potential autophagy-related molecular signatures to increase the precision of TCGA-based prognostic stratification in early-stage endometrial carcinoma.MethodsUnivariate Cox regression analysis of the TCGA-Uterine Corpus Endometrial Carcinoma cohort was used to identify autophagy-related genes associated with survival outcomes in patients with endometrial carcinoma. The candidates were analyzed by the Kaplan-Meier method. Multivariate Cox regression was used to assess whether PEA15 served as an independent prognostic factor, especially for the MSI and CN-L subtypes. We examined the correlation between PEA15 protein expression and patient survival through immunohistochemical analysis of tissue microarrays from our institutional cohort of stage I endometrial cancer patients.ResultsUnivariate analysis revealed that <i>NRG3, PEA15, DNAJB1, BAK1, DRAM1, KLHL24, ATF6, CDKN2A, MBTPS2,</i> and <i>UVRAG</i> were significantly associated with survival outcomes in early-stage endometrial carcinoma patients. Multivariate analysis established PEA15 as an independent prognostic factor. Immunohistochemical analysis of tissue microarrays revealed that elevated PEA15 expression was significantly correlated with poorer overall survival and disease-free survival. Both univariate and multivariate Cox regression confirmed high PEA15 expression as an independent prognostic factor for recurrence in patients with stage I endometrioid adenocarcinoma.ConclusionsThe autophagy-related gene PEA15 is an independent prognostic biomarker in early-stage endometrial carcinoma, improving risk stratification between the MSI and CN-L subtypes. Immunohistochemical detection has clinical potential for molecular classification, offering opportunities for personalized postoperative management strategies.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":" ","pages":"3936155251383945"},"PeriodicalIF":2.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction: Over-expression of long non-coding RNA ZEB2-AS1 may predict poor prognosis and promote the migration, invasion, and epithelial-mesenchymal transition of tumor cells in non-small cell lung cancer.","authors":"","doi":"10.1177/03936155251347004","DOIUrl":"10.1177/03936155251347004","url":null,"abstract":"","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":" ","pages":"194"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The diagnostic value of midkine as a novel serum biomarker in alpha-fetoprotein-negative hepatocellular carcinoma.","authors":"Liuyi Lu, Chunling Zhu, Liling Yi, Siting Li, Mengli Fan, Jiayao Lin, Qiliu Peng","doi":"10.1177/03936155251358265","DOIUrl":"10.1177/03936155251358265","url":null,"abstract":"<p><p>ObjectiveThe objective of this study is to investigate the correlation between the expression levels of midkine (MDK) in the serum of patients with hepatocellular carcinoma (HCC) and various clinical features, and to evaluate the diagnostic efficacy of MDK in HCC cases that are negative for alpha-fetoprotein (AFP).MethodsSerum samples from 330 patients were collected from electronic cases and divided into three groups: HCC, benign liver disease, and healthy people. Serum MDK levels in all three groups were detected by ELISA. Correlation analysis was conducted to evaluate the relationship between serum MDK and liver function indexes and other traditional tumor markers in the HCC group. The receiver operating characteristic curve was used to analyze the diagnostic utility of MDK in HCC and AFP-negative HCC. In addition, univariate and multivariate analyses were performed to determine the correlation between MDK level and tumor metastasis, and clinicopathological features.ResultsMDK is significantly elevated in negative HCC (<i>P</i> = 0.001). The serum expression level of MDK in patients with HCC was found to be positively correlated with various indicators of liver injury (<i>P</i> < 0.05). Notably, elevated MDK expression was significantly associated with the China liver cancer staging system (CNLC) stage (<i>P</i> = 0.003) and complications (<i>P</i> = 0.000). Furthermore, the CNLC stage significantly impacted patient survival and metastasis (<i>P</i> = 0.016). Specifically, we propose that high levels of MDK expression are closely linked to poor tumor prognosis.ConclusionThe risk of tumor metastasis and the likelihood of poor prognosis in patients with HCC are significantly elevated in those exhibiting high levels of MDK. MDK could serve as a novel serum diagnostic marker for patients who are negative for AFP.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":" ","pages":"166-174"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144644039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benign-malignant breast nodule discrimination model based on age, tumor-associated autoantibody, ultrasonography and mammography.","authors":"Xiaorong Yang, Tianqing Yan, Yanchun Wang, Ying Tong, Xiaolu Ma, Lin Guo, Renquan Lu","doi":"10.1177/03936155251363994","DOIUrl":"10.1177/03936155251363994","url":null,"abstract":"<p><strong>Background: </strong>Several studies have suggested an association between tumor-associated autoantibodies (TAAbs) and breast cancer. However, most research has focused on imaging techniques, with few studies examining the combined use of TAAbs and imaging to distinguish benign and malignant breast nodules.</p><p><strong>Methods: </strong>Our study included 197 women with breast nodules. We collected clinical data, preoperative breast ultrasonography, and mammography results. Serum TAAbs were detected using enzyme-linked immunosorbent assay. Logistic regression analysis assessed the ability of factors to distinguish benign from malignant nodules. Receiver operating characteristic curves were plotted, and predictive models were constructed.</p><p><strong>Results: </strong>Five TAAbs (BRCA2, TP53, ATAD2, NY-ESO-1, CAGE) exhibited significant differences (<i>p</i> < 0.001) between benign and malignant breast nodules, with specificity of 95.83% (area under the curve  = 0.722). Pearson's χ² results revealed a close association between the levels of 5-TAAbs and breast cancer stages. Additionally, preoperative 5-TAAbs combined with ultrasonography and mammography increased diagnostic accuracy of benign nodules by 10.42% and reduced misdiagnosis of malignant nodules by 14.85%. Multivariate logistic analysis identified age (<i>p</i> < 0.001), preoperative 5-TAAbs (<i>p</i> = 0.004), breast ultrasonography (<i>p</i> = 0.01), and mammography (<i>p</i> < 0.001) as independent factors for malignant nodules. Combining 5-TAAbs with age and imaging improved differentiation, achieving sensitivity of 85.42% and specificity of 94.06%.</p><p><strong>Conclusion: </strong>Tumor-associated autoantibodies, especially the 5-TAAbs, have certain clinical value in the early diagnosis of breast cancer and can serve as an effective auxiliary tool for preoperative breast ultrasonography and mammography in patients with breast nodules.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":" ","pages":"147-157"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144818132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SH3GL1 mediates B7-H3 recycling and enhances the immune escape in non-small cell lung cancer.","authors":"Sai Te Er Nu Er Lan, Chunling Liu","doi":"10.1177/03936155251367774","DOIUrl":"10.1177/03936155251367774","url":null,"abstract":"<p><p>PurposeThis study aimed to investigate the role of SH3GL1 in regulating B7-H3 expression and its impact on immune escape in non-small cell lung cancer (NSCLC).MethodsSH3GL1 and B7-H3 expression levels were analyzed in The Cancer Genome Atlas datasets and NSCLC cell lines using quantitative reverse transcription polymerase chain reaction and Western blot. SH3GL1 overexpression was performed to assess its effect on B7-H3 expression. Co-immunoprecipitation (Co-IP) and immunofluorescence (IF) were used to confirm the interaction and co-localization of SH3GL1 and B7-H3. Flow cytometry and confocal microscopy were employed to study B7-H3 endocytosis and recycling. The functional impact of SH3GL1 on immune escape was evaluated through T cell co-culture assays and in vivo tumor models.ResultsSH3GL1 and B7-H3 were significantly upregulated in NSCLC clinical samples and cell lines. SH3GL1 overexpression increased B7-H3 protein levels and promoted its recycling to the cell surface by redirecting B7-H3 away from lysosomal degradation. Co-IP and IF confirmed the physical interaction and co-localization of SH3GL1 and B7-H3. In vitro, SH3GL1 overexpression suppressed T cell proliferation, cytotoxicity, and activation while increasing immunosuppressive cytokines. In vivo, SH3GL1 overexpression accelerated tumor growth, increased Treg infiltration, and enhanced B7-H3 expression in tumor tissues.ConclusionSH3GL1 impacts B7-H3 expression and promotes immune escape in NSCLC by enhancing B7-H3 recycling and suppressing T cell function. These findings highlight SH3GL1 as a potential therapeutic target to overcome immune escape in NSCLC.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":" ","pages":"175-186"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144976320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of clinicopathological features of diffuse large B-cell lymphoma with bone marrow involvement.","authors":"Yuan Jin, Xiaofang Yao, Yahui Zhou, Linmei Zhong, Yi Yao, Jie Zhou, Zhiyu Wang, Yejiang Bao, Xu Qian","doi":"10.1177/03936155251371061","DOIUrl":"10.1177/03936155251371061","url":null,"abstract":"<p><p>BackgroundDiffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin lymphoma. The aim of this study was to comprehensively analyze the clinical, cytomorphological, and flow cytometry characteristics of DLBCL patients, with a focus on bone marrow involvement (BMI), to identify novel prognostic factors.MethodsThe clinical, cytomorphological, and flow cytometry characteristics of 220 DLBCL patients were collected from January 2017 to April 2024. Univariate and multivariate Cox regression analyses were performed to identify prognostic factors for overall survival (OS). Kaplan-Meier survival curves were generated. Logistic regression analyses were used to explore associations between clinical, cytomorphologic, and immunophenotyping features.ResultsThe median age of the patients was 63 years, with 48.6% showing positive BMI. Multivariate analysis confirmed that age, lactate dehydrogenase (LDH) level, hemoglobin (HGB) level, and platelet count (PLT) were independent prognostic factors for OS. Compared with patients without BMI, patients with BMI presented significant differences in laboratory parameters, such as lower lymphocyte counts and elevated inflammatory marker levels. Cytomorphological analysis of bone marrow smears revealed associations between specific cell characteristics (e.g., large cell size, medium cytoplasmic volume, and pseudopod protrusions) and immunophenotypic markers (e.g., CD23, CD79b, and cKappa).ConclusionAge, LDH, HGB, and PLT were identified as independent prognostic factors for OS. The integration of cytomorphological and flow cytometry data may provide additional insights into disease biology. Furthermore, this study highlighted that DLBCL patients with BMI have lower lymphocyte, PLT, and elevated levels of markers such as LDH, high-sensitivity C-reactive protein and β2-microglobulin.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":" ","pages":"187-193"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144976295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circular RNA PVT1 as a potential biomarker for human cancers: A systematic review and meta-analysis.","authors":"Xiaoyan Ma, Junheng Chen, Chunbin Zhou","doi":"10.1177/03936155251362862","DOIUrl":"10.1177/03936155251362862","url":null,"abstract":"<p><p>BackgroundCircular (circ)RNAs are essential regulators in cancer development and progression. CircPVT1, derived from exon 2 (410 nucleotides) of <i>PVT1</i> gene located at 8q24, has been widely recognized as an oncogenic circRNA frequently upregulated in various human cancers. This study aimed to assess the diagnostic accuracy of circPVT1 for human cancers.MethodsArticles published up to July 2024 were searched across four databases (PubMed, EMBASE, Web of Science, and Cochrane databases). A meta-analysis was conducted under a random effects model and the diagnostic performance was evaluated using receiver operator characteristic curve analysis. Subgroup analysis of circPVT1 in different cancer types and tissues was performed.ResultsOverall, 12 studies (1246 patients) were included for diagnostic outcome synthesis. The pooled sensitivity was 0.83 (95% CI, 0.77-0.88) and specificity of 0.80 (95% CI, 0.73-0.87), with an area under the receiver operator characteristic curve of 0.89 (95% CI, 0.86-0.91), highlighting the robust diagnostic value of circPVT1. Multiple studies have revealed that circPVT1 functions as a micro RNA sequester to modulate downstream gene expression, affecting various malignant behaviors in cancers.ConclusionThis study enhances our understanding of the role and mechanism of circPVT1 in human cancers and supports its potential as a promising diagnostic biomarker for various cancer types.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":" ","pages":"158-165"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144785858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of MEOX1 as a potential target in metabolic dysfunction-associated steatohepatitis-related liver fibrosis.","authors":"Xiaoxiao Jiao, Linying Lai, Yiting Qian, Bo Sun, Wenzhuo Yang","doi":"10.1177/03936155251335975","DOIUrl":"10.1177/03936155251335975","url":null,"abstract":"<p><p>BackgroundThe mechanisms underlying the occurrence and progression of metabolic dysfunction-associated steatohepatitis (MASH)-related liver fibrosis remains poorly understood. This study aims to identify key transcription factors involved in the development of liver fibrosis in MASH patients, thereby providing potential targets for drug discovery.MethodsMicroarray data were retrieved from liver biopsy specimens of MASH patients exhibiting varying stages of fibrosis via the Gene Expression Omnibus database. Differentially expressed transcription factors (DETFs) were identified through the application of Weighted Gene Co-expression Network Analysis. A set of in vitro and in vivo experiments were conducted to investigate the role of MEOX1 in MASH-related fibrosis. To delineate the potential mechanisms, the transcriptomic RNA sequencing (RNA-seq), Alphafold, and PyMOL were used.ResultsA total of six DETFs (MEOX1, SOX4, LEF1, SOX9, MYC, and CBX2) were identified as being positively correlated with the progression of MASH-related fibrosis. MEOX1 was increased in mouse model of MASH diet-induced liver fibrosis and hepatic stellate cells (HSCs) stimulated by transforming growth factor-β1. Knockdown of the MEOX1 markedly suppressed the activation, proliferation, and migration of HSCs. RNA-Seq analysis identified serine protease inhibitor family E member 1 (SERPINE1) as the critical target of MEOX1 within HSCs. The protein interaction sites of MEOX1 and SERPINE1 were predicted using Alphafold and PyMOL.ConclusionIn summary, as a pivotal transcription factor, MEOX1 activates HSCs via SERPINE1, thereby promoting liver fibrosis associated with MASH. Inhibition of the MEOX1-SERPINE1 pathway could offer a novel therapeutic avenue for treating MASH-related fibrosis.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":" ","pages":"133-144"},"PeriodicalIF":2.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Type IV collagen, carcinoembryonic antigen, osteopontin, and hepatocyte growth factor as biomarkers for liver metastatic colorectal cancer.","authors":"Moa Lindgren, Ingrid Ljuslinder, Pär Jonsson, Hanna Nyström","doi":"10.1177/03936155251329590","DOIUrl":"10.1177/03936155251329590","url":null,"abstract":"<p><p>IntroductionDiagnosis and monitoring of metastatic colorectal cancer (mCRC) depend on diagnostic imaging. Circulating carcinoembryonic antigen (CEA) can be analyzed but no optimal, non-invasive biomarker exists. Circulating collagen IV (COL IV) is a promising biomarker in patients with colorectal liver metastases (CLM). This study aimed to evaluate COL IV and other cancer-related and stroma-derived proteins as biomarkers for mCRC.Materials & methodsPlasma COL IV and 10 other proteins were analyzed with ELISA and Luminex multiplex assays.ResultsmCRC patients have elevated levels of circulating COL IV, CEA, interleukin-8 (IL-8), hepatocyte growth factor (HGF), cytokeratin-19 fragments (CYFRA 21-1), osteopontin (OPN), and migration inhibitory factor (MIF) compared to primary CRC (pCRC) patients. COL IV is elevated in mCRC patients compared to healthy individuals. Levels of COL IV, CEA, OPN, CYFRA 21-1, IL-8, and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) were dependent on the metastatic site. OPN, CEA, and HGF are very good at discriminating between mCRC patients and pCRC controls. COL IV is very good at distinguishing between mCRC patients and healthy controls. The combination of OPN + CEA is superior at detecting mCRC than CEA alone. High HGF and COL IV levels correlate to poor prognosis.ConclusionOPN, CEA, and HGF are potential biomarkers for mCRC. COL IV is a potential biomarker for CLM. The combination of OPN with CEA is superior to CEA alone in detecting mCRC. Levels of circulating proteins depend on metastatic localization, implying that a combination of markers is better than single markers in detecting mCRC disease. High levels of COL IV and HGF have potential prognostic value.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":" ","pages":"105-113"},"PeriodicalIF":2.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144059868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}