International Journal of Developmental Biology最新文献_第2页

Cyclooxygenase-2 plays a crucial role during myocardial patterning of developing chick. 环氧合酶-2在雏鸡心肌发育过程中起着至关重要的作用。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2022-01-01 DOI: 10.1387/ijdb.220153sb

Bhaval K Parmar, Urja R Verma, Juhi A Vaishnav, Suresh Balakrishnan

{"title":"Cyclooxygenase-2 plays a crucial role during myocardial patterning of developing chick.","authors":"Bhaval K Parmar, Urja R Verma, Juhi A Vaishnav, Suresh Balakrishnan","doi":"10.1387/ijdb.220153sb","DOIUrl":"https://doi.org/10.1387/ijdb.220153sb","url":null,"abstract":"Cyclooxygenase-2 (COX-2), a member of the Cyclooxygenase family, initiates the biosynthesis of prostanoids that regulates various cellular functions. Our pilot attempt revealed that the administration of etoricoxib, an inhibitor specific for COX-2, induces abnormal looping in the chicken heart. The present study attempts to reveal the mechanistic details of etoricoxib-induced abnormal cardiac looping. The activity of COX-2 was inhibited by administering 3.5 μg of etoricoxib into the egg's air cell on day zero of incubation. The gene and protein expression patterns of key mediators of heart development were then analyzed on day 2 (HH12) and day 3 (HH20). Reduced COX-2 activity altered the expressions of upstream regulators of organogenesis like Wnt11, BMP4, and SHH in the etoricoxib-exposed embryos. The observed expression shifts in the downstream regulators of myocardial patterning (MYOCD, HAND2, GATA4, GATA5, and GATA6) in the treated embryos corroborate the above results. In addition, the reduction in COX-2 activity hampered cardiomyocyte proliferation with a concomitant increase in the apoptosis rate. In conclusion, the collective effect of altered expression of signaling molecules of myocardial patterning and compromised cardiomyocyte turnover rate could be the reason behind the looping defects observed in the heart of etoricoxib-treated chick embryos.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":"66 7-8-9","pages":"373-381"},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9555128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evidence of Swim secretion and association with extracellular matrix in the Drosophila embryo. 果蝇胚胎中游泳分泌及其与细胞外基质关联的证据。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2022-01-01 DOI: 10.1387/ijdb.210205cz

Valeria Kaltezioti, Katerina M Vakaloglou, Aristidis S Charonis, Christos G Zervas

{"title":"Evidence of Swim secretion and association with extracellular matrix in the Drosophila embryo.","authors":"Valeria Kaltezioti, Katerina M Vakaloglou, Aristidis S Charonis, Christos G Zervas","doi":"10.1387/ijdb.210205cz","DOIUrl":"https://doi.org/10.1387/ijdb.210205cz","url":null,"abstract":"Secreted wingless-interacting protein (Swim) is the Drosophila ortholog gene of the mammalian Tubulointerstitial Nephritis Antigen like 1 (TINAGL1), also known as lipocalin-7 (LCN7), or adrenocortical zonation factor 1 (AZ-1). Swim and TINAGL1 proteins share a significant homology, including the somatomedin B and the predictive inactive C1 cysteine peptidase domains. In mammals, both TINAGL1 and its closely related homolog TINAG have been identified in basement membranes, where they may function as modulators of integrin-mediated adhesion. In Drosophila, Swim was initially identified in the eggshell matrix and was subsequently detected in the culture medium of S2 cells. Further biochemical analysis indicated that Swim binds to wingless (wg) in a lipid-dependent manner. This observation, together with RNAi-knockdown studies, suggested that Swim is an essential cofactor of wg-signalling. However, recent elegant genetic studies ruled out the possibility that Swim is required alone to facilitate wg-signalling in Drosophila, because flies without Swim are viable and fertile. Here, we use the UAS/Gal4 expression system together with confocal imaging to analyze the in vivo localization of a chimeric Swim-GFP in the developing Drosophila embryo. Our data fully support the notion that Swim is an extracellular matrix component that is secreted upon ectopic expression and preferentially associates with the basement membranes of various organs and with the specialized tendon matrix at the muscle attachment sites (MAS). Interestingly, the accumulation of Swim at the MAS does not require integrins. In conclusion, Swim is an extracellular matrix component, and Swim may exhibit overlapping functions in concert with other undefined components.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":"66 1-2-3","pages":"235-241"},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39706101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Zebrafish research in Greece: swimming against the current. 希腊的斑马鱼研究:逆流而行。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2022-01-01 DOI: 10.1387/ijdb.210129db

Dimitris Beis

{"title":"Zebrafish research in Greece: swimming against the current.","authors":"Dimitris Beis","doi":"10.1387/ijdb.210129db","DOIUrl":"https://doi.org/10.1387/ijdb.210129db","url":null,"abstract":"The zebrafish is a vertebrate model extensively used in Developmental Biology and Human Disease modeling, as it shares high genetic and physiological similarities with humans. It has become the second most popular animal model, after mice, with several advantages over the latter: zebrafish are easily housed and cared for; the cost of installing and maintaining a zebrafish facility is significantly lower than for mice; and they reproduce often and develop quickly. Using zebrafish complies with the 3Rs principles of laboratory animal use. Zebrafish embryos develop externally and are transparent, allowing for in vivo non-invasive imaging. There are many transgenic and mutant lines available that mimic most human diseases, including reporter lines for most signaling pathways. There are also several reverse genetic tools to functionally verify genes or variants of unknown significance, identified in Genome-Wide Association Studies (GWAS) or using Next Generation Sequencing (NGS) approaches. In addition, the model emerges as an invaluable whole animal platform for various stages of drug discovery efforts by exploring the possibility of creating high-throughput phenotypic-driven screens. These include phenotypic screenings, determinations of general and/or specific toxicity (cardiac, renal, hepatotoxicity etc.), and mechanism of action studies. Finally, zebrafish are able to retain their capacity to regenerate most organs during their entire life span, making them a well-established model for the study of organ regeneration. The European Zebrafish Society consists of more than 180 research labs throughout Europe. In Greece however, zebrafish use remains rather limited. Here I present here a brief historical overview of zebrafish research in Greece.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":"66 1-2-3","pages":"155-161"},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39438256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epithelial-to-mesenchymal transition of tumor cells: cancer progression and metastasis. 肿瘤细胞从上皮细胞到间质细胞的转变:癌症的进展和转移。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2022-01-01 DOI: 10.1387/ijdb.210180gk

Vasileios Vardas, Eleni Politaki, Evangelia Pantazaka, Vassilis Georgoulias, Galatea Kallergi

{"title":"Epithelial-to-mesenchymal transition of tumor cells: cancer progression and metastasis.","authors":"Vasileios Vardas, Eleni Politaki, Evangelia Pantazaka, Vassilis Georgoulias, Galatea Kallergi","doi":"10.1387/ijdb.210180gk","DOIUrl":"https://doi.org/10.1387/ijdb.210180gk","url":null,"abstract":"Detection and characterization of circulating tumor cells (CTCs) with an epithelial-to-mesenchymal transition (EMT) phenotype is very important, as it can contribute to the identification of high-risk for relapse and death patients. However, most methods underestimate CTC numbers, owing to their dependence on epithelial markers. In the current study, we evaluated the EMT phenotype in CTCs isolated from breast cancer (BC) patients, using the CellSearch system. Spiking experiments for the evaluation of the specificity and sensitivity of our method were performed using HeLa cells. Sixty-five breast cancer (BC) patients (47 early and 18 metastatic) were enrolled in the study. Vimentin is a mesenchymal marker that indicates tumoral cells acquiring invasive and malignant properties. We studied vimentin (VIM) expression using the extra channel of the CellSearch system and an anti-vimentin antibody conjugated with FITC. In our present results, we reported the percentage of circulating tumor cells that expressed vimentin in early and in metastatic breast cancer patients. Interestingly, the incidence of cells with a CK-VIM+CD45- phenotype was detected in both settings. These cells were detected in 31.4% of CK-negative (11/35) and 82.3% of CK-positive (10/12) early BC patients. The corresponding numbers for metastatic disease were 15.4% (2/13) and 100% (5/5), respectively. Our results suggest that in CTC-negative patients, potentially undetectable tumor cells could be identified using the FDA-approved CellSearch system, based on the (CK-VIM+CD45-)-phenotype, offering additional information regarding metastatic dissemination in cancer patients. Further experiments evaluating more biomarkers are necessary to elucidate the mechanisms that regulate tumorigenesis and metastasis.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":"66 1-2-3","pages":"277-283"},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39794477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Cortical interneuron development: a role for small Rho GTPases. 皮层中间神经元发育:小Rho gtpase的作用。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2022-01-01 DOI: 10.1387/ijdb.210186dk

Zouzana Kounoupa, Domna Karagogeos

{"title":"Cortical interneuron development: a role for small Rho GTPases.","authors":"Zouzana Kounoupa, Domna Karagogeos","doi":"10.1387/ijdb.210186dk","DOIUrl":"https://doi.org/10.1387/ijdb.210186dk","url":null,"abstract":"GABAergic interneurons control cortical excitation/inhibition balance and are implicated in severe neurodevelopmental disorders. In contrast to the multiplicity of signals underlying the generation and migration of cortical interneurons, the intracellular proteins mediating the response to these cues are largely unknown. We have demonstrated the unique and diverse roles of the Rho GTPases Rac1 and 3 in cell cycle and morphology in transgenic animals where Rac1 and Rac1/3 were ablated specifically in cortical interneurons. In the Rac1 mutant, progenitors delay their cell cycle exit, probably due to a prolonged G1 phase resulting in a cortex with 50% reductions in interneurons and an imbalance of excitation/inhibition in cortical circuits. This disruption in GABAergic inhibition alters glutamatergic function in the adult cortex, which could be reversed by enhancement of GABAergic functions during an early postnatal period. Furthermore, this disruption disturbs neuronal synchronization in the adult cortex. In the double mutant, additional severe cytoskeletal defects result in an 80% interneuron decrease. Both lines die postnatally from epileptic seizures. We have made progress towards characterizing the cell cycle defect in Rac1 mutant interneuron progenitors, determining the morphological and synaptic characteristics of single and double mutant interneurons and identifying some of the molecular players through which Racs exert their actions via proteomic analysis. In our present work, we review these studies and discuss open questions and future perspectives. We hope that our data will contribute to the understanding of the function of cortical interneurons, especially since preclinical models of interneuron-based cell therapies are being established.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":"66 1-2-3","pages":"35-42"},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39794479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

RNA silencing pathways in plant development and defense. RNA沉默途径在植物发育和防御中的作用。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2022-01-01 DOI: 10.1387/ijdb.210189kk

Nikoleta Kryovrysanaki, Anthony James, Martha Tselika, Eirini Bardani, Kriton Kalantidis

引用次数: 8

Developmental and regenerative biology of cardiomyocytes. 心肌细胞的发育和再生生物学。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2022-01-01 DOI: 10.1387/ijdb.210159kh

Angeliki Daiou, Katerina Petalidou, Georgios Siokatas, Eleftherios I Papadopoulos, Konstantinos E Hatzistergos

{"title":"Developmental and regenerative biology of cardiomyocytes.","authors":"Angeliki Daiou, Katerina Petalidou, Georgios Siokatas, Eleftherios I Papadopoulos, Konstantinos E Hatzistergos","doi":"10.1387/ijdb.210159kh","DOIUrl":"https://doi.org/10.1387/ijdb.210159kh","url":null,"abstract":"Current progress and challenges in understanding the molecular and cellular mechanisms of cardiomyocyte embryonic development and regeneration are reviewed in our present work. Three major topics are critically discussed: how do cardiomyocytes form in the embryo? What is the adult origin of the cells that regenerate cardiomyocytes in animal models with adult heart regeneration capabilities? Can the promise of therapeutic cardiomyocyte regeneration be realized in humans? In the first topic, we highlight current advances in understanding the developmental biology of cardiomyocytes, with emphasis on the regulative capabilities of the early embryo during specification and allocation of the cardiomyoblasts that produce the primordial heart. We place further emphasis on trabecular cardiomyocyte development from late cardiomyoblasts, neural crest cells and primordial cardiomyocytes, and their critical role in the clonal growth of the compact/septal and cortical cardiomyocyte layers in the mammalian embryo and adult zebrafish, respectively. In the second topic, we focus on the re-activation of the cortical or trabecular compaction programs as hallmarks of cardiomyocyte regenerative cells during adult zebrafish and neonatal mouse heart regeneration, respectively, and underscore the metabolic remodeling that commonly drives cardiomyocyte regeneration in these organisms. Finally, we discuss the status of preclinical and clinical-stage therapeutics for cardiomyocyte regeneration, with particular emphasis on gene therapy, as well as adult and pluripotent stem cell-based cellular cardiomyoplasty approaches. In summary, our article provides a bird's-eye view of current knowledge and potential pitfalls in the field of developmental biology-guided regenerative medicine strategies for the treatment of heart diseases.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":"66 1-2-3","pages":"59-75"},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39705317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Keratinocyte differentiation and proteolytic pathways in skin (patho) physiology. 皮肤(病理)生理学中的角质细胞分化和蛋白水解途径。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2022-01-01 DOI: 10.1387/ijdb.210161gs

Eleni Zingkou, Georgios Pampalakis, Georgia Sotiropoulou

{"title":"Keratinocyte differentiation and proteolytic pathways in skin (patho) physiology.","authors":"Eleni Zingkou, Georgios Pampalakis, Georgia Sotiropoulou","doi":"10.1387/ijdb.210161gs","DOIUrl":"https://doi.org/10.1387/ijdb.210161gs","url":null,"abstract":"The epidermis is a stratified epithelium that forms the barrier between the organism and its environment. It is mainly composed of keratinocytes at various stages of differentiation. The stratum corneum is the outermost layer of the epidermis and is formed of multiple layers of anucleated keratinocytes called corneocytes. We aim to highlight the roles of epidermal differentiation and proteolysis in skin diseases. Skin biopsies isolated from Spink5-/- mice, the established model of Netherton syndrome (NS), and from patients with NS, seborrheic dermatitis (SD) and psoriasis, as well as healthy controls, were analyzed by histology and immunohistochemistry. Our results showed that NS, SD, and psoriasis are all characterized by abnormal epidermal differentiation, manifested by hyperplasia, hyperkeratosis, and parakeratosis. At the molecular level, abnormal differentiation is accompanied by increased expression of involucrin and decreased expression of loricrin in NS and psoriasis. Increased epidermal proteolysis associated with increased kallikrein-related peptidases (KLKs) expression is also observed in both NS and psoriatic epidermis. Furthermore, reduced expression of desmosomal proteins is observed in NS, but increased in psoriasis. Since desmosomal proteins are proteolytic substrates and control keratinocyte differentiation, their altered expression directly links epidermal proteolysis to differentiation. In conclusion, abnormal cellular differentiation and proteolysis are interconnected and underlie the pathology of NS, SD and psoriasis.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":"66 1-2-3","pages":"269-275"},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39705318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Expression of Hey marks a subset of enteroendocrine cells in the Drosophila embryonic and larval midgut. Hey的表达标志着果蝇胚胎和幼虫中肠肠内分泌细胞的一个亚群。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2022-01-01 DOI: 10.1387/ijdb.210203mm

Emilia Skafida, Christos Delidakis, Maria Monastirioti

{"title":"Expression of Hey marks a subset of enteroendocrine cells in the Drosophila embryonic and larval midgut.","authors":"Emilia Skafida, Christos Delidakis, Maria Monastirioti","doi":"10.1387/ijdb.210203mm","DOIUrl":"https://doi.org/10.1387/ijdb.210203mm","url":null,"abstract":"Hey is a conserved transcription factor of the bHLH-Orange family that participates in the response to Notch signaling in certain tissues. Whereas three Hey paralogues exist in mammalian genomes, Drosophila possesses a single Hey gene. Fly Hey is expressed in the subset of newborn neurons that receive a Notch signal to differentiate them from their sibling cells after the asymmetric division of precursors called ganglion-mother-cells. We used a polyclonal anti-Hey serum and a GFP-tagged transgenic duplication of the Hey locus to examine its expression in tissues outside the nervous system in embryos and larvae. We detected robust Hey expression in the embryonic midgut primordium at the time of birth of enteroendocrine cells, identified by expression of Prospero. Approximately half of the Pros-positive cells were also Hey positive at mid-embryogenesis. By the end of embryogenesis, most enteroendocrine cells had downregulated Hey expression, although it was still detectable at low levels after hatching. Low levels of Hey were also detected in subsets of the epithelial enterocytes at different times. Embryo enteroendocrine Hey expression was found to be Notch dependent. In late third-instar larvae, when few new enteroendocrine cells are born, novel Hey expression was detected in one cell of each sibling pair. In conclusion, Hey is strongly expressed in one of each pair of newly-born enteroendocrine cells. This is consistent with a hypothesis that embryonic enteroendocrine cells are born by an asymmetric division of a precursor, where Notch/Hey probably distinguish between the subtypes of these cells upon their differentiation.","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":"66 1-2-3","pages":"223-233"},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39706099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vascular cell-matrix adhesion in development and cancer. 血管细胞-基质粘附在发育和肿瘤中的作用。

IF 0.7 4区生物学

International Journal of Developmental Biology Pub Date : 2022-01-01 DOI: 10.1387/ijdb.210204vk

Christina Arapatzi, Georgia Rouni, Vassiliki Kostourou

引用次数: 1