Emerging Science Journal最新文献

{"title":"Immobilization of Aspergillus fumigatus α-Amylase via Adsorption onto Bentonite/Chitosan for Stability Enhancement","authors":"Y. Yandri, Hendri Ropingi, T. Suhartati, Bambang Irawan, S. Hadi","doi":"10.28991/esj-2023-07-05-023","DOIUrl":"https://doi.org/10.28991/esj-2023-07-05-023","url":null,"abstract":"Stability enhancement attempted in this study demonstrated that significant improvement in the stability of the α-amylase isolated from Aspergillus fumigatus was achieved by immobilizing the enzyme on a bentonite/chitosan hybrid matrix using the adsorption method. Centrifugation was used to isolate the α-amylase, which was then refined using (NH4)2SO4 salt precipitation and dialysis. The purity of the α-amylase improved 19.40 times when compared to that of the crude extract. The optimal temperature for free α-amylase is 50˚C, while the optimum temperature for α-amylase/bentonite/chitosan is 60˚C. The KM value of α-amylase/bentonite/chitosan was 1.69 ± 0.08 mg mL-1 substrate and the Vmax value was 52.32 ± 3.29 µmol mL-1 min-1, whereas for free α-amylase, the KM value of 2.56 ± 0.09 mg mL-1 substrate and the Vmax value of 3.78 ± 0.09 µmol mL-1 min-1 were obtained. The ΔGi value of free α-amylase is 102.68 ± 0.30 kJ mol-1 and the t½ is 21.23 ± 0.23 min, whereas the ΔGi value of α-amylase/bentonite/chitosan is 104.43 ± 0.00 kJ mol-1 and the t½ is 94.29 ± 0.91 min. The higher values of ΔGi and t½demonstrated that α-amylase/bentonite/chitosan has better stability than that of free α-amylase. Another important finding is that α-amylase /bentonite/chitosan was able to retain their activity as high as 47.61 ± 0.53% after six recycles, indicating that the enzyme has the potential to be used in industry. Doi: 10.28991/ESJ-2023-07-05-023 Full Text: PDF","PeriodicalId":502658,"journal":{"name":"Emerging Science Journal","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139327907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of COVID-19 on Family Support for Home-Schooling in Urban Areas","authors":"Osman S. Ahmed, Asma Ahmed, M. Eltahir, Salah Gad, Ali S. Ahmed, Zezit M. Noufal, Alaa Zuhir Al Rawashdeh","doi":"10.28991/esj-2023-07-05-021","DOIUrl":"https://doi.org/10.28991/esj-2023-07-05-021","url":null,"abstract":"The objective of the study is to clarify that the family participated in COVID-19 as a home-schooling family. The sample was selected according to the geographical distribution of schools in the eastern region of Khartoum. Data was collected through a questionnaire and interviews. The most important result is that there is an increasing interest from parents to support home-schooling despite the challenges of continuing learning, such as the widening digital divide in technology, previous experience, and poor network connectivity. This research paper focused on the point of view of parents in the city of Khartoum on supporting home-schooling according to social variables related to both mother and father, and the paper concluded that parents of students in private schools are more supportive of home education. Also, most of the parents of students who support home-schooling have reached their university level of education and post-university and belong to the youth age group. Although parents emphasize the importance of home-schooling during the COVID-19 infection, there are challenges associated with providing home-schooling for their children. Doi: 10.28991/ESJ-2023-07-05-021 Full Text: PDF","PeriodicalId":502658,"journal":{"name":"Emerging Science Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139329892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Therapy Protocols and Vaccines Applied in the COVID-19 Pandemic","authors":"G. Ozbey, A. Hanafiah, A. Sukri, František Zigo, J. Arvaiová","doi":"10.28991/esj-2023-07-05-022","DOIUrl":"https://doi.org/10.28991/esj-2023-07-05-022","url":null,"abstract":"Despite a plethora of research on vaccine developments and the adverse effects of the vaccineworldwide, there are several research studies on COVID-19 vaccine hesitancy or rejection. The objectives of this study were to investigate the impact of the COVID-19 vaccine on treatment and the factors that contributed to the vaccine concerns. Methods: After one month of being approved by the ethics committee, a descriptive cross-sectional research was conducted using online resources. Samples were randomly selected from the study population. The current study included adults (>18 years old) who were randomly selected from different provinces of Turkey. Results: Most participants were young adults 18–23 years old (47.9%), female (64.8%), married (36.1%), with university education (70.4%), and without medical illness (60.9%) (mean age = 28 years old). Both vaccination doses were given to 53% of the individuals. The most frequent symptom was discomfort at the injection site (0.14%), followed by asthenia muscle pain (0.01%) and edema (24.5%) at the injection site. More symptoms were reported by women than by men. Social media accounts and websites of professional organizations, namely the Dental Association, the Ministry of Health, and the World Health Organization (WHO), were the main sources of COVID-19 information. Hesitancy was induced by concerns about side effects (67.7%), safety (69.7%), lack of information (43.1%), inadequate data on the vaccine clinical trials (55.9%), lack of information on how long protective and effectiveness of vaccines (69.4%), lack of information on how effectiveness against variants (74.8%), think COVID-19 is not dangerous (69.7%), not effectiveness (22.6%) of the vaccine, not safe (32.6%), change my genetic make-up and cause it reproductive sterilite (20.2%), high number of deaths due to COVID-19 (60.8%), the presence of dangerous materials such as aluminum, mercury and others in the content of vaccines, concerns about the country producing the vaccine (38.2%), being afraid of the injection (22%), being against vaccination in general (11%), negative news in the press and social media (38.6%), being risky for only people over 60 (15.9%), having a strong immune system (38.4%), religious (4.7%) and cultural factors (7.4%), believing in natural and traditional medicine (25.4%), and being influenced by friends or family members (19.6%). Hesitancy to accept the COVID-19 vaccine was observed in subjects with hypercholosterolemia and diabetes mellitus. In conclusion, Vaccine hesitancy was primarily related to safety apprehensions. Educational and demographic traits were associated with vaccine admissibility. Doi: 10.28991/ESJ-2023-07-05-022 Full Text: PDF","PeriodicalId":502658,"journal":{"name":"Emerging Science Journal","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139328403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}