Journal of Investigational Allergology and Clinical Immunology最新文献

{"title":"Jun o 1, a Cross-reactive Juniperus oxycedrus Allergen With Unique Structural and Immunogenic Properties.","authors":"Sandra Sivill, Marcos Viñuela, Irene Real-Arévalo, Miriam Morán-Morales, Lucia Moreno-Serna, Celia López-Sanz, Emilio Nuñez-Borque, Rodrigo Jiménez-Saiz, Jose C Hernández-Walias, Sarai Martínez-Cano, Juan M Igea, Jose A Lemus Calderon, Ana M Navarro-Pulido, Carmen Rondon, Enrique Fernandez-Caldas, Fernando Pineda, Jose L Subiza, Salvador Iborra, Jose F Cantillo","doi":"10.18176/jiaci.1186","DOIUrl":"https://doi.org/10.18176/jiaci.1186","url":null,"abstract":"<p><strong>Background and objective: </strong>Juniperus oxycedrus, from the Cupressaceae family, is widespread in the Mediterranean. However, the allergenic relevance of its pollen remains underexplored. Objective: This study aimed to characterize Jun o 1, an allergen of J oxycedrus that is homologous to major allergens in related species.</p><p><strong>Methods: </strong>Native Jun o 1, its nonglycosylated Escherichia coli-expressed form (rJun o 1), and Cup a 1 were purified by affinity chromatography. Jun o 1 was characterized by SDS-PAGE, 2D-electrophoresis, Western blot (WB), and mass spectrometry and compared with the homologous cypress allergen, Cup a 1. Enzymatically and chemically deglycosylated Jun o 1 was also analyzed. Specific IgE binding and cross-reactivity were evaluated using ELISA, WB, and mast cell activation assays with sera from 57 Spanish cypress-allergic patients. IgG binding was tested using sera from rJun o 1-immunized mice.</p><p><strong>Results: </strong>Jun o 1 exists as multiple isoforms and assembles into oligomers, whereas Cup a 1 comprises multiple isoforms without oligomerization. IgE binding to Jun o 1, including mast cell activation, is critically influenced by the combined presence of protein and carbohydrate determinants. Compared to Cup a 1, Jun o 1 exhibited greater IgE-binding inhibition for J oxycedrus extract in sera from Cupressaceae-sensitized allergic patients from this tree's autochthonous regions. The highest rate of monosensitization in this group was to J oxycedrus.</p><p><strong>Conclusion: </strong>Jun o 1 is a relevant and potential major allergen in cypress allergy. Structural and immunogenic similarities with Cup a 1 lead to cross-reactivity, although it also exhibits unique properties, resulting from combined IgE-reactive protein and glycan determinants.</p>","PeriodicalId":50173,"journal":{"name":"Journal of Investigational Allergology and Clinical Immunology","volume":" ","pages":"0"},"PeriodicalIF":4.8,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Retrospective Multicenter Cohort Study on the Effectiveness of Triple Inhaled Therapy in Severe Asthma.","authors":"David Dacal Rivas, Nagore Blanco Cid, Daniel Pérez Ortiz, Mar Mosteiro Añon, Coral Gonzalez Fernández, Jose M Alvarez Torres, Abel Pallares Sanmartin, Abraham Ali-Munive, Carlos A Torres-Duque, Raquel Ruiz Ferreras, Andrés Blanco Hortas, Carmen Vidal, Luis Pérez de Llano","doi":"10.18176/jiaci.1181","DOIUrl":"https://doi.org/10.18176/jiaci.1181","url":null,"abstract":"<p><strong>Background and objectives: </strong>Triple therapy (TT) is effective for asthma patients whose disease remains uncontrolled with dual therapy. However, relevant questions remain unanswered. Objectives: To evaluate the long-term effectiveness of TT in a real-world setting; to determine the extent to which it prevents escalation to oral corticosteroids (OCS), azithromycin, or biologics; and to identify factors related to failure.</p><p><strong>Methods: </strong>Retrospective multicenter cohort study. Data were collected at baseline, 16-24 weeks, 52 weeks, and at the last visit. Follow-up extended to treatment escalation or the last visit. Effectiveness of TT was defined as no treatment escalation plus asthma control (Asthma Control Test [ACT] ≥20 and no severe exacerbations in the preceding 12 months).</p><p><strong>Results: </strong>A total of 390 patients were analyzed (median follow-up 40.0 months). Of these, 83 (22.5%) escalated treatment; TT was effective in 54% at the last visit. Complete remission (control plus FEV1 ≥80%) was achieved in 20.6%. Severe exacerbations, OCS load, symptoms, and FEV1 improved significantly. The factors associated with failure of TT in the previous year were smoking history (HR 1.84), lack of asthma control at 16-24 weeks (HR 3.41), bronchiectasis (HR 1.91), baseline ACT ≤15 (HR 1.81), ≥2 severe exacerbations in the previous year (HR 2.01), and low eosinophil count (HR 2.58). FEV1 declined >30 mL/year in 25.3% of patients in whom TT was effective.</p><p><strong>Conclusions: </strong>TT achieved sustained control in a significant proportion of patients whose disease was not controlled with dual therapy. Bronchiectasis, smoking history, greater clinical severity at initiation of TT, and lack of control at 16-24 weeks predicted poorer outcome.</p>","PeriodicalId":50173,"journal":{"name":"Journal of Investigational Allergology and Clinical Immunology","volume":" ","pages":"0"},"PeriodicalIF":4.8,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consensus Review: Predicting Recurrence in Chronic Rhinosinusitis With Nasal Polyps: Multidisciplinary Insights From the RELAPSE Project.","authors":"Isam Alobid, Sandra Domínguez, Christian Calvo-Henriquez, Carlos Colás, Beatriz Fernández-Parra, Ruperto González-Pérez","doi":"10.18176/jiaci.1176","DOIUrl":"https://doi.org/10.18176/jiaci.1176","url":null,"abstract":"<p><p>Chronic rhinosinusitis with nasal polyps (CRSwNP) is a disease with a high incidence of postoperative recurrence despite medical and surgical treatments. However, a universal definition of recurrence and its determinants is lacking. Our objective was to establish a clear definition of recurrence and reach consensus on the contributing factors. A consensus study was conducted using the Delphi method. The literature on recurrence of CRSwNP was reviewed and assessed by a multidisciplinary scientific committee. The committee designed a 2-round online Delphi questionnaire addressing recurrence in CRSwNP. As for consensus, ≥70% of panelists scored 1-3 (disagreement) or 7-9 (agreement). The survey involved 70 physicians (69 completed both rounds) with expertise in the management of CRSwNP (47 ear, nose, and throat [ENT] specialists and 22 allergists). Consensus was reached on the characteristics defining recurrence (87% overall agreement, 95% agreement among allergists, and 83% agreement among ENT specialists). Finally, a list of 10 key prognostic factors was agreed upon. The top 5 were presence of nonsteroidal anti-inflammatory drug-exacerbated respiratory disease, eosinophilic CRSwNP, asthma, extent of surgery, and eosinophils in nasal polyposis (>50/high-power field). This study identified 10 key prognostic factors for recurrence and clarified the definition of recurrence in CRSwNP as the reappearance or worsening of sinonasal symptoms (>3 points on a visual analog scale and/or ≥12 points in the 22-item Sino-Nasal Outcome Test) and/or objective evidence of inflammation (nasal polyposis grade ≥1 or other endoscopic or radiological signs of persistent mucosal inflammation) after initial postoperative improvement (baseline postoperative assessment 1 month after surgery, recurrence evaluated 6 months thereafter), excluding persistence and suboptimal surgical outcome.</p>","PeriodicalId":50173,"journal":{"name":"Journal of Investigational Allergology and Clinical Immunology","volume":" ","pages":"0"},"PeriodicalIF":4.8,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147787879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Drug Target in Allergic Diseases: Bruton Tyrosine Kinase.","authors":"Moisés Labrador-Horrillo, Bruno Cenni, Marta Ferrer Puga","doi":"10.18176/jiaci.1183","DOIUrl":"https://doi.org/10.18176/jiaci.1183","url":null,"abstract":"<p><p>Though first recognized as a signaling molecule in B cells, Bruton tyrosine kinase (BTK) has been shown to play a crucial role in signal transduction in innate and adaptive immune cells. BTK is an attractive therapeutic target, given its diverse role in immune regulation. Development of the first-generation BTK inhibitor (BTKi), ibrutinib, revolutionized the treatment of B-cell malignancies. Since its approval, newer-generation BTKis with improved pharmacological properties have been developed, with higher selectivity for BTK and fewer off-target effects than ibrutinib. BTK is essential for IgE-driven allergic responses and may influence IgE antibody production by B cells. Activation of BTK via the high-affinity Fc receptor for IgE, FcεRI, results in mast cell and basophil degranulation and release of histamine and other inflammatory mediators. BTKis are a potential novel therapeutic option in patients with mast cell-driven autoimmune and allergic diseases. This review summarizes the current evidence for BTK in urticaria (chronic spontaneous and chronic inducible urticaria [CSU/CIndU]), asthma, food allergy, and atopic dermatitis (AD). It also highlights the efficacy of BTKis in urticaria and allergic diseases where FcεRI/IgE is the pathogenic target. Based on limited available data, BTK does not seem to have a prominent role in the pathogenesis of AD, and its role in asthma is unclear, thus necessitating further clinical research. Remibrutinib and rilzabrutinib have shown the most promise in allergic and autoimmune diseases to date; remibrutinib is approved in adults with CSU, and rilzabrutinib is approved in adults with persistent or chronic immune thrombocytopenia.</p>","PeriodicalId":50173,"journal":{"name":"Journal of Investigational Allergology and Clinical Immunology","volume":" ","pages":"0"},"PeriodicalIF":4.8,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147787916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remission of Eosinophilic Otitis Media With Tezepelumab.","authors":"Diana Betancor, William Aragonés Sanzen-Baker, Virginia Vasallo, Jaime Bernaola, Jesica Santillan, Jose M Villacampa, Joaquin Sastre","doi":"10.18176/jiaci.1177","DOIUrl":"https://doi.org/10.18176/jiaci.1177","url":null,"abstract":"","PeriodicalId":50173,"journal":{"name":"Journal of Investigational Allergology and Clinical Immunology","volume":" ","pages":"0"},"PeriodicalIF":4.8,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147787901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tezepelumab in Allergic Bronchopulmonary Aspergillosis With Severe Uncontrolled Asthma: A Retrospective 4-Case Series.","authors":"Belén Sanz-Sanjosé, Daniel Laorden, David Romero, Leticia De Las Vecillas, Elena Villamañán, Javier Domínguez-Ortega, Santiago Quirce, Rodolfo Álvarez-Sala","doi":"10.18176/jiaci.1173","DOIUrl":"https://doi.org/10.18176/jiaci.1173","url":null,"abstract":"","PeriodicalId":50173,"journal":{"name":"Journal of Investigational Allergology and Clinical Immunology","volume":" ","pages":"0"},"PeriodicalIF":4.8,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147787947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitellogenin Identified as a Novel Allergen in Tenebrio molitor: Evidence from an Occupational Asthma Case.","authors":"Leyre Baptista-Serna, Manuel De Las Heras-Gozalo, Dinara Korshunova, Elena Santano, Maria J Martínez, Fernando Pineda, Jose A Cañas, Victoria Del Pozo, Joaquín Sastre","doi":"10.18176/jiaci.1175","DOIUrl":"https://doi.org/10.18176/jiaci.1175","url":null,"abstract":"","PeriodicalId":50173,"journal":{"name":"Journal of Investigational Allergology and Clinical Immunology","volume":" ","pages":"0"},"PeriodicalIF":4.8,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147700637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the Tajogaite Volcanic Eruption on Respiratory and Emotional Health in Allergic Patients: A Prospective Study.","authors":"Zulay Almeida-Sánchez, Paula Jiménez-Gallardo, Paula Aznárez-Rodríguez, Teresa Carrillo-Diaz, Felipe Rodríguez de Castro","doi":"10.18176/jiaci.1182","DOIUrl":"https://doi.org/10.18176/jiaci.1182","url":null,"abstract":"","PeriodicalId":50173,"journal":{"name":"Journal of Investigational Allergology and Clinical Immunology","volume":" ","pages":"0"},"PeriodicalIF":4.8,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147700607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regular Allergen Exposure During Oral Immunotherapy Alters the Transcriptomic Innate Immune Response After Cellular Restimulation in Children With Egg Allergy.","authors":"Victoria I Hinkkanen, Terhi Savinko, Kati Palosuo, Harri Alenius, Mika J Mäkelä, Piia Karisola","doi":"10.18176/jiaci.1079","DOIUrl":"10.18176/jiaci.1079","url":null,"abstract":"<p><strong>Background and objective: </strong>Oral immunotherapy (OIT) is a promising treatment for food allergies. However, the molecular mechanisms leading to desensitization remain unknown. Objective: To better understand the immunological mechanisms and transcriptional changes underlying desensitization to food allergens during OIT.</p><p><strong>Methods: </strong>Our cohort consisted of 40 Finnish children with egg allergy who underwent OIT. Peripheral blood mononuclear cells (PBMCs) were collected at 0, 3, and 8 months of therapy and stimulated with an egg allergen extract. Differentially expressed genes (DEGs) were identified based on quantile-normalized and batch-corrected microarray data using a linear model. Gene enrichment and Pearson correlation analyses were conducted.</p><p><strong>Results: </strong>After 8 months of therapy, 45% of patients were fully desensitized and 55% partially desensitized. Stimulation with egg yielded 49 DEGs at 0 months, 723 DEGs at 3 months, and 759 DEGs at 8 months in PBMCs after comparison with unstimulated controls. At 8 months of OIT, allergen stimulation led to down-regulation of proinflammatory pathways, as well as IL-4 and IL-13 signaling. At baseline, the immune response in the fully desensitized group was more reactive than in the partially desensitized group.</p><p><strong>Conclusions: </strong>During OIT, general immune activity is increased, especially the number of down-regulated genes, suggesting active immune suppression. Transcriptomic profiles differ between fully and partially desensitized patients, with a notably more reactive immune response in the fully desensitized group at baseline. Innate immunity seems to play a significant role in the development of desensitization during OIT.</p>","PeriodicalId":50173,"journal":{"name":"Journal of Investigational Allergology and Clinical Immunology","volume":" ","pages":"132-142"},"PeriodicalIF":4.8,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144057006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IgE-Mediated Hypersensitivity to Olaparib Diagnosed and Monitored With the Basophil Activation Test.","authors":"Ana Morales-Hidalgo, Alicia Gómez-López, Jose A Cañas, Marcela Valverde-Monge, Carlos Villalobos-Vilda, Victoria Del Pozo Abejón, Joaquín Sastre","doi":"10.18176/jiaci.1140","DOIUrl":"10.18176/jiaci.1140","url":null,"abstract":"","PeriodicalId":50173,"journal":{"name":"Journal of Investigational Allergology and Clinical Immunology","volume":" ","pages":"160-162"},"PeriodicalIF":4.8,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146144568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}