npj Biological Physics and Mechanics最新文献

Mechanical signatures in cancer metastasis. 癌症转移的机械特征。

npj Biological Physics and Mechanics Pub Date : 2025-01-01 Epub Date: 2025-02-04 DOI: 10.1038/s44341-024-00007-x

Ayushi Agrawal, Yousef Javanmardi, Sara A Watson, Bianca Serwinski, Boris Djordjevic, Wenbin Li, Amir R Aref, Russell W Jenkins, Emad Moeendarbary

引用次数: 0

Exploring effects of platelet contractility on the kinetics, thermodynamics, and mechanisms of fibrin clot contraction. 探讨血小板收缩对纤维蛋白凝块收缩的动力学、热力学和机制的影响。

npj Biological Physics and Mechanics Pub Date : 2025-01-01 Epub Date: 2025-02-24 DOI: 10.1038/s44341-025-00011-9

Evgenii Kliuchnikov, Alina D Peshkova, Minh Quan Vo, Kenneth A Marx, Rustem I Litvinov, John W Weisel, Prashant K Purohit, Valeri Barsegov

{"title":"Exploring effects of platelet contractility on the kinetics, thermodynamics, and mechanisms of fibrin clot contraction.","authors":"Evgenii Kliuchnikov, Alina D Peshkova, Minh Quan Vo, Kenneth A Marx, Rustem I Litvinov, John W Weisel, Prashant K Purohit, Valeri Barsegov","doi":"10.1038/s44341-025-00011-9","DOIUrl":"10.1038/s44341-025-00011-9","url":null,"abstract":"Mechanisms of blood clot contraction - platelet-driven fibrin network remodeling, are not fully understood. We developed a detailed computational ClotDynaMo model of fibrin network with activated platelets, whose clot contraction rate for normal 450,000/µl human platelets depends on serum viscosity η, platelet filopodia length l, and weakly depends on filopodia traction force f and filopodia extension-retraction speed v. Final clot volume is independent of η, but depends on v, f and l. Analysis of ClotDynaMo output revealed a 2.24 TJ/mol clot contraction free energy change, with ~67% entropy and ~33% internal energy changes. The results illuminate the \"optimal contraction principle\" that maximizes volume change while minimizing energy cost. An 8-chain continuum model of polymer elasticity containing platelet forces, captures clot contractility as a function of platelet count, η and l. The ClotDynaMo and continuum models can be extended to include red blood cells, variable platelet properties, and mechanics of fibrin network.","PeriodicalId":501703,"journal":{"name":"npj Biological Physics and Mechanics","volume":"2 1","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Speckle fluctuations reveal dynamics of microparticles in fibrin scaffolds in a model of bacterial infection. 斑点波动揭示了细菌感染模型中纤维蛋白支架中微粒的动力学。

npj Biological Physics and Mechanics Pub Date : 2025-01-01 Epub Date: 2025-06-03 DOI: 10.1038/s44341-025-00019-1

Nichaluk Leartprapun, Anton Deti, Brandon C Matthews, Ziqian Zeng, Nathaniel Hai, Seemantini K Nadkarni

{"title":"Speckle fluctuations reveal dynamics of microparticles in fibrin scaffolds in a model of bacterial infection.","authors":"Nichaluk Leartprapun, Anton Deti, Brandon C Matthews, Ziqian Zeng, Nathaniel Hai, Seemantini K Nadkarni","doi":"10.1038/s44341-025-00019-1","DOIUrl":"10.1038/s44341-025-00019-1","url":null,"abstract":"Fibrin plays an important role in both immune response and pathogen virulence during bacterial infection. Pathogens such as staphylococci interact with fibrin through dynamic processes, involving binding, entrapment, and release from fibrin scaffolds, through which they experience an evolving cascade of nano- to microscale dynamics that span broad timescales. Yet, the biophysical dynamics that unfold between invading bacteria and host fibrin are not well understood. Here, we present a non-invasive optical approach based on speckle fluctuation to characterize the multiscale dynamics of microparticles in fibrin scaffolds. Using microparticles of varying sizes and surface chemistry to emulate spherical, non-motile bacterial cells, we demonstrate real-time monitoring of bacteria-fibrin interactions during fibrin clot formation and fibrinolysis in purified fibrins scaffolds and clinical plasma clots. Our approach circumvents the need for sophisticated position tracking equipment, making it potentially applicable to a broad range of experimental systems for biophysical investigation of bacteria-extracellular network interactions.","PeriodicalId":501703,"journal":{"name":"npj Biological Physics and Mechanics","volume":"2 1","pages":"15"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12133583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physical principles and mechanisms of cell migration. 细胞迁移的物理原理和机制。

npj Biological Physics and Mechanics Pub Date : 2025-01-01 Epub Date: 2025-01-16 DOI: 10.1038/s44341-024-00008-w

Roberto Alonso-Matilla, Paolo P Provenzano, David J Odde

引用次数: 0

Cellular mechanisms of traumatic brain injury. 创伤性脑损伤的细胞机制。

npj Biological Physics and Mechanics Pub Date : 2025-01-01 Epub Date: 2025-06-03 DOI: 10.1038/s44341-025-00020-8

Gia Kang, Eng Kuan Moo, Rohan Banton, Oren E Petel, Andrew R Harris

{"title":"Cellular mechanisms of traumatic brain injury.","authors":"Gia Kang, Eng Kuan Moo, Rohan Banton, Oren E Petel, Andrew R Harris","doi":"10.1038/s44341-025-00020-8","DOIUrl":"10.1038/s44341-025-00020-8","url":null,"abstract":"Mild traumatic brain injury (mTBI) is an acute injury with immediate and medium-term symptom presentation. However, our mechanistic understanding of mTBI and how mechanical loading of soft cellular tissues leads to injury is limited. The aim of this review is to introduce this interdisciplinary field to non-experts and provide an overview of our current understanding of how mechanical trauma contributes to cellular injury. Here, we compare the significance of various measures of mechanical loading including strain magnitude, strain rate, loading mode, and frequency, and their relative significance for cell and tissue injury in in vitro and ex vivo experimental models reported in the literature. Interestingly, while it is difficult to define a precise injury threshold value based on strain magnitude alone, cellular injury is commonly observed at strain rates of >0.1 s-1, higher than rates observed in many normal cell functions (< 0.01 s-1). We explore the role of the plasma membrane, cytoskeleton, and specialized structures in maintaining cell integrity during traumatic injury.","PeriodicalId":501703,"journal":{"name":"npj Biological Physics and Mechanics","volume":"2 1","pages":"16"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12133593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relaxation of mucosal fibronectin fibers in late gut inflammation following neutrophil infiltration in mice. 小鼠中性粒细胞浸润后的晚期肠道炎症中粘膜纤维连接蛋白的松弛。

npj Biological Physics and Mechanics Pub Date : 2025-01-01 Epub Date: 2025-02-04 DOI: 10.1038/s44341-024-00006-y

Ronja Rappold, Konstantinos Kalogeropoulos, Gianna La Regina, Ulrich Auf dem Keller, Emma Slack, Viola Vogel

{"title":"Relaxation of mucosal fibronectin fibers in late gut inflammation following neutrophil infiltration in mice.","authors":"Ronja Rappold, Konstantinos Kalogeropoulos, Gianna La Regina, Ulrich Auf dem Keller, Emma Slack, Viola Vogel","doi":"10.1038/s44341-024-00006-y","DOIUrl":"10.1038/s44341-024-00006-y","url":null,"abstract":"The continuously remodeled extracellular matrix (ECM) plays a pivotal role in gastrointestinal health and disease, yet its precise functions remain elusive. In this study, we employed laser capture microdissection combined with low-input proteomics to investigate ECM remodeling during Salmonella-driven inflammation. To complement this, we probed how fibronectin fiber tension is altered using a mechanosensitive peptide probe. While fibronectin fibers in healthy intestinal tissue are typically stretched, many lose their tension in intestinal smooth muscles only hours after infection, despite the absence of bacteria in that area. In contrast, within the mucosa, where Salmonella is present starting 12 h post infection, fibronectin fiber relaxation occurred exclusively during late-stage infection at 72 h and was localized to already existing clusters of infiltrated neutrophils. Using N-terminomics, we identified three new cleavage sites in fibronectin in the inflamed cecum. The unique, tissue layer-specific changes in the molecular compositions and ECM fiber tension revealed herein might trigger new therapeutic strategies to fight acute intestinal inflammation.","PeriodicalId":501703,"journal":{"name":"npj Biological Physics and Mechanics","volume":"2 1","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11794144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of extracellular matrix viscoelasticity in development and disease. 细胞外基质粘弹性在发育和疾病中的作用。

npj Biological Physics and Mechanics Pub Date : 2025-01-01 Epub Date: 2025-04-03 DOI: 10.1038/s44341-025-00014-6

Olivia Courbot, Alberto Elosegui-Artola

引用次数: 0

Unifying fragmented perspectives with additive deep learning for high-dimensional models from partial faceted datasets. 将碎片化视角与来自部分面数据集的高维模型的加性深度学习统一起来。

npj Biological Physics and Mechanics Pub Date : 2025-01-01 Epub Date: 2025-02-24 DOI: 10.1038/s44341-025-00009-3

Yufei Wu, Pei-Hsun Wu, Allison Chambliss, Denis Wirtz, Sean X Sun

引用次数: 0

Mechanobiology in the eye. 眼睛的机械生物学。

npj Biological Physics and Mechanics Pub Date : 2025-01-01 Epub Date: 2025-07-04 DOI: 10.1038/s44341-025-00022-6

C Ross Ethier, Samuel Herberg

{"title":"Mechanobiology in the eye.","authors":"C Ross Ethier, Samuel Herberg","doi":"10.1038/s44341-025-00022-6","DOIUrl":"10.1038/s44341-025-00022-6","url":null,"abstract":"The eye presents a very dynamic biomechanical environment, and thus ocular cells must be highly mechanosensitive and mechanoresponsive. Moreover, defects in mechanobiological pathways contribute to a number of sight-threatening ocular diseases, highlighting the importance of ocular mechanobiology. We here give a concise overview of the mechanobiology of ocular cells in the lens and cornea (and how mechanobiology plays a role in associated pathologies in these tissues), before providing a detailed review of the mechanobiology of the common blinding disease, glaucoma. Mechanical stimuli are intimately linked with the pathology of glaucoma, both in terms of altered homeostasis of the eye's internal pressure control system and in the response of neural cells to elevated pressure in the eye. A complex array of mechanosensory elements (stretch-activated ion channels, integrins, G protein-coupled receptors) work together with intersecting networks of mechanotransducing pathways in cells of both the posterior and anterior eye in glaucoma. Despite intense research efforts over the past decades, much remains unknown about the mechanobiology of glaucoma. Continued investigation of glaucomatous mechanobiology is important, as it may reveal novel targets for treating this challenging disease.","PeriodicalId":501703,"journal":{"name":"npj Biological Physics and Mechanics","volume":"2 1","pages":"18"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The synergistic potential of mechanotherapy and sonopermeation to enhance cancer treatment effectiveness. 机械治疗和超声手术的协同潜力，以提高癌症的治疗效果。

npj Biological Physics and Mechanics Pub Date : 2025-01-01 Epub Date: 2025-05-05 DOI: 10.1038/s44341-025-00017-3

Constantina Neophytou, Triantafyllos Stylianopoulos, Fotios Mpekris

引用次数: 0