bioRxiv - Physiology最新文献_第6页

The transaminase-omega-amidase pathway is a redox switch in glutamine metabolism that generates alpha-ketoglutarate 转氨酶-ω-酰胺酶途径是谷氨酰胺代谢的氧化还原开关，可产生α-酮戊二酸

bioRxiv - Physiology Pub Date : 2024-08-29 DOI: 10.1101/2024.08.28.610061

Niklas Herrle, Pedro Felipe Malacarne, Timothy Warwick, Alfredo Cabrera-Orefice, Yiheng Chen, Maedeh Gheisari, Souradeep Chatterjee, Matthias S. Leisegang, Tamim Sarakpi, Sarah Wionski, Melina Lopez, Ina Koch, Marcus Kessler, Sabine Klein, Frank Erhard Uschner, Jonel Trebicka, Steffen Brunst, Ewgenij Proschak, Stefan Guenther, Monica Rosas-Lemus, Nina Baumgarten, Stephan Klatt, Thimoteus Speer, Ilka Wittig, Marcel H. Schulz, J. Brent Richards, Ralf Gilsbach, Travis T. Denton, Ingrid Fleming, Luciana Hannibal, Ralf P. Brandes, Flavia Rezende

{"title":"The transaminase-omega-amidase pathway is a redox switch in glutamine metabolism that generates alpha-ketoglutarate","authors":"Niklas Herrle, Pedro Felipe Malacarne, Timothy Warwick, Alfredo Cabrera-Orefice, Yiheng Chen, Maedeh Gheisari, Souradeep Chatterjee, Matthias S. Leisegang, Tamim Sarakpi, Sarah Wionski, Melina Lopez, Ina Koch, Marcus Kessler, Sabine Klein, Frank Erhard Uschner, Jonel Trebicka, Steffen Brunst, Ewgenij Proschak, Stefan Guenther, Monica Rosas-Lemus, Nina Baumgarten, Stephan Klatt, Thimoteus Speer, Ilka Wittig, Marcel H. Schulz, J. Brent Richards, Ralf Gilsbach, Travis T. Denton, Ingrid Fleming, Luciana Hannibal, Ralf P. Brandes, Flavia Rezende","doi":"10.1101/2024.08.28.610061","DOIUrl":"https://doi.org/10.1101/2024.08.28.610061","url":null,"abstract":"Oxidative stress is caused by short-lived molecules and metabolic changes belong to the fastest cellular responses. Here we studied how the endothelial cell metabolome reacts to acute oxidative challenges (menadione or H2O2) to identify redox-sensitive metabolic enzymes. H2O2 selectively increased alpha-ketoglutaramate (alphaKGM), a largely uncharacterized metabolite produced by glutamine transamination and a yet unrecognized intermediate of endothelial glutamine catabolism. The enzyme nitrilase-like 2 omega-amidase (NIT2) converts alphaKGM to alpha-ketoglutarate (alphaKG). Reversible oxidation of specific cysteine in NIT2 by H2O2 inhibited its catalytic activity. Furthermore, a variant in the NIT2 gene that decreases its expression is associated with high plasma alphaKGM level in humans. Endothelial-specific knockout mice of NIT2 exhibited increased levels of alphaKGM and impaired angiogenesis. Knockout of NIT2 impaired endothelial cell proliferation and sprouting and induced senescence. In conclusion, we show that the glutamine transaminase-omega-amidase pathway is a metabolic switch in which NIT2 is the redox-sensitive enzyme. The pathway is modulated in humans and functionally important for endothelial glutamine metabolism.","PeriodicalId":501557,"journal":{"name":"bioRxiv - Physiology","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective autophagy of ribosomes balances a tradeoff between starvation survival and growth resumption 核糖体的选择性自噬平衡了饥饿生存和恢复生长之间的权衡

bioRxiv - Physiology Pub Date : 2024-08-29 DOI: 10.1101/2024.08.28.609383

Joel Tuomaala, Devanarayanan Siva Sankar, Julie Perey, Sacha Psalmon, Nicholas Stroustrup, Joern Dengjel, Benjamin D Towbin

{"title":"Selective autophagy of ribosomes balances a tradeoff between starvation survival and growth resumption","authors":"Joel Tuomaala, Devanarayanan Siva Sankar, Julie Perey, Sacha Psalmon, Nicholas Stroustrup, Joern Dengjel, Benjamin D Towbin","doi":"10.1101/2024.08.28.609383","DOIUrl":"https://doi.org/10.1101/2024.08.28.609383","url":null,"abstract":"In environments with fluctuating nutrient abundance, organisms must survive periods of starvation, yet quickly resume growth upon food encounter. A tradeoff between these objectives is well-documented in microbes, where it is caused by the need to partition the total cellular protein content between growth- and survival-enhancing proteins. However, the molecular mechanisms of growth-survival tradeoffs in multicellular animals remain largely unknown. Here, we addressed this mechanism for C. elegans by measuring the dynamic changes of its proteome during starvation using live imaging and proteomics. We found that starved animals catabolize ribosomal proteins through autophagy, which provides essential energy for survival while preserving organismal integrity. However, the resulting decline in ribosomes delayed growth resumption upon refeeding until pre-starvation ribosome levels were restored. Genetic inhibition of ribosomal autophagy had a dual effect: although it accelerated growth after short starvation, it compromised survival during prolonged starvation. These findings reveal the rate of ribosomal catabolism as a key determinant of a tradeoff between starvation survival and rapid growth resumption whose tuning may adapt animals to different starvation durations. Our research shows how the need to balance protein allocation between growth and survival constrains animal physiology, highlighting the mechanistic role of proteome resource limitation in whole-organism tradeoffs.","PeriodicalId":501557,"journal":{"name":"bioRxiv - Physiology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fetal liver neutrophils are responsible for the postnatal neutrophil surge 胎儿肝脏中性粒细胞是出生后中性粒细胞激增的罪魁祸首

bioRxiv - Physiology Pub Date : 2024-08-28 DOI: 10.1101/2024.08.26.609612

Ryo Ishiwata, Yuji Morimoto

{"title":"Fetal liver neutrophils are responsible for the postnatal neutrophil surge","authors":"Ryo Ishiwata, Yuji Morimoto","doi":"10.1101/2024.08.26.609612","DOIUrl":"https://doi.org/10.1101/2024.08.26.609612","url":null,"abstract":"Mammalian neonates experience an abrupt surge of blood neutrophil count within the first day of life. The postnatal neutrophil surge is regarded as a defensive reaction against infection; however, the mechanisms underlying this surge remain unclear.\u0000The present study demonstrates that the postnatal neutrophil surge arises from the liver neutrophil pool. In rat neonates, the neutrophil surge was evident at 6 hours after birth. The proportion and immaturity of bone marrow neutrophils remained unaltered at 6 hours but increased only after the surge had peaked. In the rat fetal and neonatal livers, we observed prenatal neutrophil accumulation and acute loss of the neutrophils coinciding with the postnatal neutrophil surge. In Lys-EGFP mice, an acute loss of liver neutrophils was observed within 12 hours of birth. This loss was characterized by a decrease in mature neutrophils and by perivascular neutrophil localization in the livers. Additionally, mouse fetuses exhibited an accumulation of the liver neutrophil pool during the late gestational period (e15-18), which was attributable to neutrophil-biased myeloid differentiation mediated by granulocyte-colony stimulating factor (G-CSF). The liver neutrophils exhibited characteristic transcriptomic alterations within three hours of birth, exemplified by an increase in the Nos2 (iNOS) gene. The administration of a non-selective NOS inhibitor or an iNOS-selective inhibitor resulted in the inhibition of the postnatal neutrophil surge in rat neonates, accompanied by the retention of liver neutrophils. These findings shed light on the previously unidentified source of the postnatal neutrophil surge and the stimulus initiating it.","PeriodicalId":501557,"journal":{"name":"bioRxiv - Physiology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal progesterone and adipose mPRε in pregnancy regulate the embryonic nutritional state 孕期母体孕酮和脂肪 mPRε 调节胚胎营养状况

bioRxiv - Physiology Pub Date : 2024-08-27 DOI: 10.1101/2024.08.26.609823

Keita Watanabe, Mayu Yamano, Junki Miyamoto, Ryuji Ohue-Kitano, Yuki Masujima, Daiki Sasahara, Yuki Mouri, Nozomu Kono, Shunsuke Inuki, Fumitaka Osakada, Kentaro Nagaoka, Junken Aoki, Yuki Sugiura, Hiroaki Ohno, Eiji Kondoh, Ikuo Kimura

{"title":"Maternal progesterone and adipose mPRε in pregnancy regulate the embryonic nutritional state","authors":"Keita Watanabe, Mayu Yamano, Junki Miyamoto, Ryuji Ohue-Kitano, Yuki Masujima, Daiki Sasahara, Yuki Mouri, Nozomu Kono, Shunsuke Inuki, Fumitaka Osakada, Kentaro Nagaoka, Junken Aoki, Yuki Sugiura, Hiroaki Ohno, Eiji Kondoh, Ikuo Kimura","doi":"10.1101/2024.08.26.609823","DOIUrl":"https://doi.org/10.1101/2024.08.26.609823","url":null,"abstract":"Sex steroid hormones such as progesterone play a pivotal role in reproductive functions and maintaining pregnancy; however, the impact of progesterone on the interaction between mother and embryo is unclear. Here, we demonstrate that the relationship between maternal progesterone and membrane progesterone receptor epsilon (mPRε) in adipose tissue regulates embryonic nutritional environment and growth after birth in mice. The activation of adipose mPRε by increased progesterone during pregnancy enhanced maternal insulin resistance through the production of prostaglandins, thereby efficiently providing glucose to embryos. The offspring of mPRε-deficient mothers exhibited metabolic dysfunction, whereas mPRε-deficient mothers with high-fat-diet-induced obesity exhibited improved insulin sensitivity. These findings establish the importance of progesterone as a nutritional regulator between mother and embryo, and suggest that mPRε modulators could be developed to treat pregnant glycemic control disorders such as gestational diabetes mellitus, as well as metabolic syndrome in offspring.","PeriodicalId":501557,"journal":{"name":"bioRxiv - Physiology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycoplasma gallisepticum (MG) infection inhibits mitochondrial respiratory function in a wild songbird 五倍子支原体（MG）感染抑制野生鸣禽的线粒体呼吸功能

bioRxiv - Physiology Pub Date : 2024-08-27 DOI: 10.1101/2024.08.26.609819

Chidambaram Ramanathan, Elina Thomas, Amberleigh E. Henschen, James S. Adelman, Yufeng Zhang

{"title":"Mycoplasma gallisepticum (MG) infection inhibits mitochondrial respiratory function in a wild songbird","authors":"Chidambaram Ramanathan, Elina Thomas, Amberleigh E. Henschen, James S. Adelman, Yufeng Zhang","doi":"10.1101/2024.08.26.609819","DOIUrl":"https://doi.org/10.1101/2024.08.26.609819","url":null,"abstract":"An animals immune function is vital for survival, but some pathogens could manipulate their hosts immune and metabolic responses. One example is Mycoplasma gallisepticum (MG), which infects both the respiratory system and conjunctiva of the eye in house finches (Haemorhous mexicanus). MG has been shown to exhibit immune- and metabolic-suppressive properties, but the physiological mechanisms underlying these properties are still unknown. Recent studies demonstrated that mitochondria could serve as powerhouses for both ATP production and immunity, notably inflammatory processes, through regulating complex II and its metabolites. Consequently, in this study, we investigate the short-term (3d post inoculation) and long-term (34d post inoculation) effects of MG infection on the hepatic mitochondrial respiration of house finches from two populations and infected with two different MG isolates. After short-term infection, MG-infected birds had significantly lower state 2 and state 4 respiration, but only when using complex II substrates. After long-term infection, MG-infected birds exhibited lower state 3 respiration with both complex I and II substrates, resulting in lower respiratory control ratio compared to uninfected controls, which aligned with the hypothesized metabolic-suppressive properties of MG. Interestingly, mitochondrial respiration showed limited differences with house finch population of origin, MG isolate, and whether birds were recovered from infection or not. We propose that MG may target mitochondrial complex II for its immune-suppressive properties during the early stages of infection and inhibit mitochondrial respiration for its metabolic-suppressive properties at later stage of infection, both of which should delay recovery of the host and extend infectious periods.","PeriodicalId":501557,"journal":{"name":"bioRxiv - Physiology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rate of force development is correlated with corticospinal excitability during explosive voluntary contractions. 在爆发性自主收缩过程中，力量发展速度与皮质脊髓兴奋性相关。

bioRxiv - Physiology Pub Date : 2024-08-27 DOI: 10.1101/2024.08.27.607589

Federico Castelli, Omar S Mian, Adam Bruton, Ashika Chembila Valappil, Ricci Hannah, Neale Anthony Tillin

{"title":"Rate of force development is correlated with corticospinal excitability during explosive voluntary contractions.","authors":"Federico Castelli, Omar S Mian, Adam Bruton, Ashika Chembila Valappil, Ricci Hannah, Neale Anthony Tillin","doi":"10.1101/2024.08.27.607589","DOIUrl":"https://doi.org/10.1101/2024.08.27.607589","url":null,"abstract":"Objective: To investigate the relationship between rate of torque development (RTD) and corticospinal excitability (denoted by motor-evoked potential; MEP) during explosive voluntary contractions. Also, to assess differences in MEP and silent period duration between different phases of explosive contraction and at maximum voluntary contraction (MVC) plateau. Methods: In 14 adults, quadriceps muscle MEP and silent period duration were measured at ~45 (early), ~115 (middle), and ~190 ms (late) from EMG onset during knee-extensor isometric explosive contractions, and at MVC plateau with superimposed transcranial magnetic stimulation (TMS). RTD was measured immediately prior to early-phase MEP during the same contractions, and these two variables were correlated across separate contractions, within participants, via repeated measures correlation (RmCorr). RTD was also measured in explosive contractions without TMS over early-, middle-, and late-phases and correlated to MEP averaged across the corresponding and preceding phases, via Pearson's r correlations, assessing relationships across participants. MEP and silent period duration were compared (ANOVA) between the different phases and at MVC plateau. Results: MEP and RTD were correlated across separate contractions within participants (RmCorr r=0.43), and in the middle phase across participants (Pearson's r=0.56). MEP and RTD were not correlated in other phases (r≥0.09). Silent period duration increased throughout the different phases of contraction and up to MVC plateau (ANOVA, p= 0.001) but MEP remained constant (ANOVA, p= 0.42). Conclusion: The correlations between MEP and RTD suggests corticospinal excitability is an important determinant of RTD. During rapid torque development and up to MVC plateau, corticospinal inhibition increases but excitability remains constant.","PeriodicalId":501557,"journal":{"name":"bioRxiv - Physiology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Test-retest reliability of TMS motor evoked responses and silent periods during explosive voluntary isometric contractions. 在爆发性自主等长收缩过程中，TMS 运动诱发反应和静默期的测试-再测试可靠性。

bioRxiv - Physiology Pub Date : 2024-08-27 DOI: 10.1101/2024.08.12.607577

Federico Castelli, Omar Sahid Mian, Adam Bruton, Ashika C Chembila Valappil, Neale Anthony Tillin

{"title":"Test-retest reliability of TMS motor evoked responses and silent periods during explosive voluntary isometric contractions.","authors":"Federico Castelli, Omar Sahid Mian, Adam Bruton, Ashika C Chembila Valappil, Neale Anthony Tillin","doi":"10.1101/2024.08.12.607577","DOIUrl":"https://doi.org/10.1101/2024.08.12.607577","url":null,"abstract":"Purpose: This study assessed the test-retest reliability of TMS motor evoked potentials (MEPs) and silent periods at early, middle, and late phases of the rising time-torque curve during explosive voluntary contractions. We also investigated how the number of consecutively averaged measurements influenced reliability. Methods: On two separate occasions 3-7 days apart, 14 adults completed 48 isometric explosives (1-s) contractions of the knee extensors, superimposed with TMS. The TMS elicited an MEP and silent period in the superficial quadriceps muscles at 45 (early), 115 (middle), or 190 ms (late) during each contraction. TMS was also superimposed at the plateau of 15 separate MVCs. Test-retest intraclass correlation coefficient (ICC) and coefficient of variation (CV) were calculated for MEPs and silent periods consecutively averaged over 3 to 15 separate contractions. Results: No one condition/phase was more reliable than another. For MEP amplitude, in all conditions except the explosive late phase, ICCs generally increased, and CV decreased, with an increase in the number of averaged contractions, and were >0.50 ICC and <15% CV within 7 contractions. For silent period, ICCs and CVs were unaffected by the number of consecutively averaged contractions and remained >0.50 ICC and <10% CV. Conclusion: Test-retest reliability of TMS responses is comparable between phases of explosive contraction and at the plateau of MVC. To maximise reliability of MEPs during explosive contractions or MVCs, we recommend future studies average data across more than the 3-5 contractions typically reported in the literature investigating MEPs at MVC plateau.","PeriodicalId":501557,"journal":{"name":"bioRxiv - Physiology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential role of taurine in acetaminophen hepatotoxic induced rats 牛磺酸在对乙酰氨基酚肝毒性大鼠中的潜在作用

bioRxiv - Physiology Pub Date : 2024-08-26 DOI: 10.1101/2024.08.23.609394

Isaac Olamide Babalola, Habeeb Kehinde Adekunle, Muhammed Damola Adigun

{"title":"The potential role of taurine in acetaminophen hepatotoxic induced rats","authors":"Isaac Olamide Babalola, Habeeb Kehinde Adekunle, Muhammed Damola Adigun","doi":"10.1101/2024.08.23.609394","DOIUrl":"https://doi.org/10.1101/2024.08.23.609394","url":null,"abstract":"Taurine demonstrates an important cytoprotective function in the body, regulating oxidative and inflammation in various conditions. Taurine is actively synthesized in the hepatocyte, moreover, it confers protection to oxidative mediated injury in the liver. Acetaminophen, which has been shown to trigger oxidative related liver damage, is administered in its hepatotoxic dose to rats in this study. The preventive and regulatory role of taurine is investigated in this study. Twenty five adult male wistar rats were grouped into 5 distinct groups labeled the positive control group, negative control group, groups administered with taurine 12 hours prior acetaminophen administration, simultaneously with acetaminophen and an hour after acetaminophen was administered. The study indicated that taurine significantly reduced acetaminophen-induced liver injury in an in vivo rat model. Adding taurine either 12 hours before or at the time of acetaminophen treatment effectively prevented hepatocyte necrosis. Moreover, administering taurine 1 hour after the onset of acetaminophen-induced hepatotoxicity significantly improved liver injury, as evidenced by reduced hepatocyte necrosis, possibly through its unique cytoprotective properties such as antioxidant activity. Keywords: Taurine, Acetaminophen, Hepatotoxicity, Antioxidant","PeriodicalId":501557,"journal":{"name":"bioRxiv - Physiology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Affordable and Customizable Arduino-based Thermo-controller for Thermal Ecology Experiments 用于热生态实验的经济实惠且可定制的基于 Arduino 的热控制器

bioRxiv - Physiology Pub Date : 2024-08-26 DOI: 10.1101/2024.08.23.609058

Cassidy Hawk, Erik Iverson, Justin C Havird

引用次数: 0

A marsh samphire (Salicornia europaea) extract induces a strictly sex-specific, Tor-signaling-dependent extension of lifespan and exhibits anti-obesogenic properties 一种沼泽桑寄生（Salicornia europaea）提取物可诱导严格性别特异性的、依赖于 Tor 信号的寿命延长，并具有抗致肥胖特性

bioRxiv - Physiology Pub Date : 2024-08-26 DOI: 10.1101/2024.08.25.609591

Navid Tahan Zadeh, Miram Knop, Lisa Marie Ulrich, Iris Bruchhaus, Roman Lang, Kai Luersen, Gerald Rimbach, Thomas Roeder

{"title":"A marsh samphire (Salicornia europaea) extract induces a strictly sex-specific, Tor-signaling-dependent extension of lifespan and exhibits anti-obesogenic properties","authors":"Navid Tahan Zadeh, Miram Knop, Lisa Marie Ulrich, Iris Bruchhaus, Roman Lang, Kai Luersen, Gerald Rimbach, Thomas Roeder","doi":"10.1101/2024.08.25.609591","DOIUrl":"https://doi.org/10.1101/2024.08.25.609591","url":null,"abstract":"We have studied the health-promoting properties of the marsh samphire Salicornia europaea by employing the fruit fly Drosophila melanogaster. Supplementing a standard diet with 0.2% of an aqueous extract of this halophilic plant (SEE), which grows in sandy and muddy habitats in intertidal zones, extended the lifespan of two different Drosophila strains by up to a third. This effect was strictly sex-specific and only affected females. When analyzing the body composition, we found that the body fat content of SEE-treated female flies was lower compared to controls. The extract also positively impacted the lifespan of flies fed a high-fat diet but not a high-sugar diet. It is of note in this context that SEE exhibited a lipase-inhibitory activity in vitro. Moreover, SEE counteracted ageing-associated loss of intestinal barrier integrity. The sex-specific lifespan extensions induced by the SEE were entirely dependent on functional Tor signaling in the flies. Tissue-specific silencing of the Tor signalling pathway in different cellular compartments of the intestine reduced, but did not completely abolish, the lifespan-prolonging effect in females. This suggests that other organs also contribute to this effect. In contrast to the observation that Tor signalling is necessary for the life-prolonging effect, Foxo deficiency led to a reduction in the effect, but not to its complete absence. In conclusion, the SEE is a promising candidate for a health-promoting intervention, and we laid the current groundwork for identifying the compounds that mediate this effect in future studies.","PeriodicalId":501557,"journal":{"name":"bioRxiv - Physiology","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142197774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0