The Crop JournalPub Date : 2024-03-07DOI: 10.1016/j.cj.2024.02.001
Wenyu Li, Han Wang, Qiuyue Xu, Long Zhang, Yan Wang, Yongbiao Yu, Xiangkun Guo, Zhiwei Zhang, Yongbin Dong, Yuling Li
{"title":"The cytosolic isoform of triosephosphate isomerase, ZmTPI4, is required for kernel development and starch synthesis in maize (Zea mays L.)","authors":"Wenyu Li, Han Wang, Qiuyue Xu, Long Zhang, Yan Wang, Yongbiao Yu, Xiangkun Guo, Zhiwei Zhang, Yongbin Dong, Yuling Li","doi":"10.1016/j.cj.2024.02.001","DOIUrl":"https://doi.org/10.1016/j.cj.2024.02.001","url":null,"abstract":"Triosephosphate isomerase (TPI) is an enzyme that functions in plant energy production, accumulation, and conversion. To understand its function in maize, we characterized a maize TPI mutant, . In comparison to the wild type, mutants showed altered ear development, reduced kernel weight and starch content, modified starch granule morphology, and altered amylose and amylopectin content. Protein, ATP, and pyruvate contents were reduced, indicating ZmTPI4 was involved in glycolysis. Although subcellular localization confirmed ZmTPI4 as a cytosolic rather than a plastid isoform of TPI, the mutant showed reduced leaf size and chlorophyll content. Overexpression of in led to enlarged leaves and increased seed weight, suggesting a positive regulatory role of ZmTPI4 in kernel weight and starch content. We conclude that ZmTPI4 functions in maize kernel development, starch synthesis, glycolysis, and photosynthesis.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"135 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140073766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SsdchA is a novel secretory cellobiohydrolase driving pathogenicity in Sclerotinia sclerotiorum","authors":"Yangui Chen, Yijuan Ding, Siqi Zhao, Nan Yang, Zhaohui Wu, Ping Zhang, Hongmei Liao, Mengquan Dong, Yang Yu, Huafang Wan, Wei Qian","doi":"10.1016/j.cj.2024.01.016","DOIUrl":"https://doi.org/10.1016/j.cj.2024.01.016","url":null,"abstract":"The necrotrophic fungus, , employs an array of cell wall-degrading enzymes (CWDEs), including cellulase, to dismantle host cell walls. However, the molecular mechanisms through which degrades cellulose remain elusive. Here, we unveil a novel secretory cellobiohydrolase, SsdchA, characterized by a signal peptide and a Glyco_hydro_7 (GH7) domain. exhibits a robust expression of during early infection stages. Interestingly, colony morphology and growth rates remain unaffected across the wild-type, deletion strains and overexpression strains on potato dextrose agar (PDA) medium. Nevertheless, the pathogenicity and cellobiohydrolase activity decreased in the deletion strains, but enhanced in the overexpression strains. Moreover, the heterologous expression of in leads to reduced cellulose content and heightened susceptibility to . Collectively, our data underscore the pivotal role of the novel cellobiohydrolase SsdchA in the pathogenicity of","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140073689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mapping of powdery mildew resistance genes transferred to common wheat from wild emmer wheat revealed three functional Pm60 haplotypes","authors":"Wenxin Wei, Nannan Liu, Shengnan Zhang, Jing Zhang, Wei Pan, Xiaoming Xie, Zuhuan Yang, Junna Sun, Jun Ma, Zhaorong Hu, Weilong Guo, Qiaoling Luo, Jingzhong Xie, Fei He, Yinghui Li, Chaojie Xie, Qixin Sun","doi":"10.1016/j.cj.2024.01.015","DOIUrl":"https://doi.org/10.1016/j.cj.2024.01.015","url":null,"abstract":"Powdery mildew (PM), caused by f. sp (), is one of the destructive wheat diseases worldwide. Wild emmer wheat ( ssp. , WEW), a tetraploid progenitor of common wheat, is a valuable genetic resource for wheat disease resistance breeding programs. We developed three hexaploid pre-breeding lines with PM resistance genes derived from three WEW accessions. These resistant pre-breeding lines were crossed with susceptible common wheat accessions. Segregations in the F populations were 3 resistant: 1 susceptible, suggesting a single dominant allele in each resistant parent. Mapping of the resistance gene in each line indicated a single locus on the long arm of chromosome 7A, at the approximate location of previously cloned from . Sanger sequencing revealed three different haplotypes (Hap 3, Hap 5, and Hap 6). Co-segregating diagnostic markers were developed for identification and selection of each haplotype. The resistance function of each haplotype was verified by the virus-induced gene silencing (VIGS). Common wheat lines carrying each of these haplotypes were resistant to most isolates and differences in the response arrays suggested allelic variation in response.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140019373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Suppressing a mitochondrial calcium uniporter activates the calcium signaling pathway and promotes cell elongation in cotton","authors":"Yujia Duan, Xiaoguang Shang, Ruiping Tian, Weixi Li, Xiaohui Song, Dayong Zhang, Wangzhen Guo","doi":"10.1016/j.cj.2024.01.014","DOIUrl":"https://doi.org/10.1016/j.cj.2024.01.014","url":null,"abstract":"Mitochondrial calcium uniporter (MCU) is a conserved calcium ion (Ca) transporter in the mitochondrial inner membrane of eukaryotic cells. How MCU proteins regulate Ca flow and modulate plant cell development remain largely unclear. Here, we identified the gene encoding a MCU protein that negatively regulates plant development and fiber elongation in cotton (). expressed constitutively in various tissues with the higher transcripts in elongating fiber cells. Knockdown of in cotton significantly elevated the plant height and root length. The calcium signaling pathway was significantly activated and calcium sensor genes, including Ca dependent modulator of interactor of constitutively active ROP () calmodulin like protein (, calcium-dependent protein kinases (), calcineurin B-like protein (), and CBL-interacting protein kinases (), were dramatically upregulated in silenced plants. Metabolic processes were preferentially enriched, and genes related to regulation of transcription were upregulated in silenced plants. The contents of Ca and HO were significantly increased in roots and leaves of silenced plants. Fiber length and Ca and HO contents in fibers were significantly increased in silenced plants. This study indicated that plays a negative role in regulating cell elongation in cotton, thus expanding understanding in the role of MCU proteins in plant growth and development.","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139951605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Crop JournalPub Date : 2024-02-22DOI: 10.1016/j.cj.2024.01.013
Zhuang Li, Qichao Tu, Xiangguang Lyu, Qican Cheng, Ronghuan Ji, Chao Qin, Jun Liu, Bin Liu, Hongyu Li, Tao Zhao
{"title":"GmSTF accumulation mediated by DELLA protein GmRGAs contributes to coordinating light and gibberellin signaling to reduce plant height in soybean","authors":"Zhuang Li, Qichao Tu, Xiangguang Lyu, Qican Cheng, Ronghuan Ji, Chao Qin, Jun Liu, Bin Liu, Hongyu Li, Tao Zhao","doi":"10.1016/j.cj.2024.01.013","DOIUrl":"https://doi.org/10.1016/j.cj.2024.01.013","url":null,"abstract":"Plant height influences plant architecture, lodging resistance, and yield performance. It is modulated by gibberellic acid (GA) metabolism and signaling. DELLA proteins, acting as central repressors of GA signaling, integrate various environmental and hormonal signals to regulate plant growth and development in .","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139951603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Crop JournalPub Date : 2024-02-20DOI: 10.1016/j.cj.2024.01.012
Jingli Ding, Chenchen Ji, Lu Yu, Chuang Wang, Guangda Ding, Sheliang Wang, Lei Shi, Fangsen Xu, Hongmei Cai
{"title":"OsMYB84, a transcriptional regulator of OsCOPT2 and OsHMA5, modulates copper uptake and transport and yield production in rice","authors":"Jingli Ding, Chenchen Ji, Lu Yu, Chuang Wang, Guangda Ding, Sheliang Wang, Lei Shi, Fangsen Xu, Hongmei Cai","doi":"10.1016/j.cj.2024.01.012","DOIUrl":"https://doi.org/10.1016/j.cj.2024.01.012","url":null,"abstract":"","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"270 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139921839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Crop JournalPub Date : 2024-02-07DOI: 10.1016/j.cj.2024.01.002
Linhua Wu, Min Hu, Shuwei Lyu, Wenfeng Chen, Hang Yu, Qing Liu, Wei He, Chen Li, Zuofeng Zhu
{"title":"The occurrence, inheritance, and segregation of complex genomic structural variation in synthetic Brassica napus","authors":"Linhua Wu, Min Hu, Shuwei Lyu, Wenfeng Chen, Hang Yu, Qing Liu, Wei He, Chen Li, Zuofeng Zhu","doi":"10.1016/j.cj.2024.01.002","DOIUrl":"https://doi.org/10.1016/j.cj.2024.01.002","url":null,"abstract":"<p>Panicle architecture is an agronomic determinant of crop yield and a target for cereal crop improvement. To investigate its molecular mechanisms in rice, we performed map-based cloning and characterization of <em>OPEN PANICLE 1</em> (<em>OP1</em>), a gain-of-function allele of <em>LIGULELESS 1</em> (<em>LG1</em>), controlling the spread-panicle phenotype. This allele results from a 48-bp deletion in the <em>LG1</em> upstream region and promotes pulvinus development at the base of the primary branch. Increased <em>OP1</em> expression and altered panicle phenotype in pC1 chimeric transgenic plants and upstream-region knockout mutants indicated that the deletion regulates spread-panicle architecture in the mutant <em>spread panicle 1</em> (<em>sp1</em>). Knockout <em>BRASSINOSTEROID UPREGULATED1</em> (<em>BU1</em>) gene in the background of <em>OP1</em> complementary plants showed compact panicles, suggesting <em>OP1</em> may regulate inflorescence architecture via the brassinosteroid signaling pathway. We propose that manipulating the upstream regulatory region of <em>OP1</em> or changing BR signal pathway could be an efficient way to improve rice inflorescence architecture.</p>","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139769303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Making wheat salt tolerant: What is missing?","authors":"Lukasz Kotula, Noreen Zahra, Muhammad Farooq, Sergey Shabala, Kadambot H.M. Siddique","doi":"10.1016/j.cj.2024.01.005","DOIUrl":"https://doi.org/10.1016/j.cj.2024.01.005","url":null,"abstract":"<p>The growing global population presents a significant challenge to ensuring food security, further compounded by the increasing threat of salinity to agricultural productivity. Wheat, a major staple food providing 20 % of the total caloric intake for humans, is susceptible to salinity stress. Developing new salt-tolerant wheat cultivars using wheat breeding techniques and genetic modifications is crucial to addressing this issue while ensuring the sustainability and efficiency of wheat production systems within the prevailing climate trend. This review overviews the current landscape in this field and explores key mechanisms and associated genetic traits that warrant attention within breeding programs. We contend that traditional approaches to breeding wheat for Na<sup>+</sup> exclusion have limited applicability across varying soil salinity levels, rendering them inefficient. Moreover, we question current phenotyping approaches, advocating for a shift from whole-plant assessments to cell-based phenotyping platforms. Finally, we propose a broader use of wild wheat relatives and various breeding strategies to tap into their germplasm pool for inclusion in wheat breeding programs.</p>","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139670033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Crop JournalPub Date : 2024-02-02DOI: 10.1016/j.cj.2024.01.004
Caihua Qin, Xing Fan, Qianqian Fang, Lan Ni, Mingyi Jiang
{"title":"The CBL-interacting protein kinase OsCIPK1 phosphorylated by SAPK10 positively regulates responses to ABA and osmotic stress in rice","authors":"Caihua Qin, Xing Fan, Qianqian Fang, Lan Ni, Mingyi Jiang","doi":"10.1016/j.cj.2024.01.004","DOIUrl":"https://doi.org/10.1016/j.cj.2024.01.004","url":null,"abstract":"<p>Subclass III sucrose nonfermenting1-related protein kinase 2s (SnRK2s) function in ABA and abiotic stress responses by unknown mechanisms. We found that osmotic stress/ABA-activated protein kinase 10 (SAPK10), a member of rice SnRK2s, physically interacted with CBL-interacting protein kinase 1 (OsCIPK1). <em>OsCIPK1</em> expression was up-regulated by ABA and PEG treatment, and overexpression increased the ABA sensitivity of seed germination and root growth and plant osmotic stress tolerance. Osmotic stress or ABA-induced activation of OsCIPK1 is dependent on SAPK10. SAPK10 phosphorylates Thr-24 of OsCIPK1 <em>in vitro</em>, and this phosphorylation increases the activity of OsCIPK1 and positively regulates the function of OsCIPK1 in ABA responses and plant osmotic stress tolerance. This study suggests that OsCIPK1 is a direct phosphorylated substrate of SAPK10, and SAPK10-mediated phosphorylation of OsCIPK1 functions in ABA signaling and increases rice osmotic stress tolerance.</p>","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139669827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Crop JournalPub Date : 2024-01-30DOI: 10.1016/j.cj.2024.01.003
Yuhang Zhang, Haiping Du, Tiantian Zhao, Chunmei Liao, Tu Feng, Jun Qin, Baohui Liu, Fanjiang Kong, Zhijun Che, Liyu Chen
{"title":"Corrigendum to “GmTOC1b negatively regulates resistance to Soybean mosaic virus” [Crop J. 11 (2023) 1762–1773]","authors":"Yuhang Zhang, Haiping Du, Tiantian Zhao, Chunmei Liao, Tu Feng, Jun Qin, Baohui Liu, Fanjiang Kong, Zhijun Che, Liyu Chen","doi":"10.1016/j.cj.2024.01.003","DOIUrl":"https://doi.org/10.1016/j.cj.2024.01.003","url":null,"abstract":"Abstract not available","PeriodicalId":501058,"journal":{"name":"The Crop Journal","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139644746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}