The EMBO Journal最新文献_第3页

An Orai1 gain-of-function tubular aggregate myopathy mouse model phenocopies key features of the human disease. Orai1功能获得性肾小管聚集性肌病小鼠模型表现出人类疾病的主要特征。

The EMBO Journal Pub Date : 2024-10-17 DOI: 10.1038/s44318-024-00273-4

Nan Zhao,Antonio Michelucci,Laura Pietrangelo,Sundeep Malik,Linda Groom,Jennifer Leigh,Thomas N O'Connor,Takahiro Takano,Paul D Kingsley,James Palis,Simona Boncompagni,Feliciano Protasi,Robert T Dirksen

{"title":"An Orai1 gain-of-function tubular aggregate myopathy mouse model phenocopies key features of the human disease.","authors":"Nan Zhao,Antonio Michelucci,Laura Pietrangelo,Sundeep Malik,Linda Groom,Jennifer Leigh,Thomas N O'Connor,Takahiro Takano,Paul D Kingsley,James Palis,Simona Boncompagni,Feliciano Protasi,Robert T Dirksen","doi":"10.1038/s44318-024-00273-4","DOIUrl":"https://doi.org/10.1038/s44318-024-00273-4","url":null,"abstract":"Tubular aggregate myopathy (TAM) is a heritable myopathy primarily characterized by progressive muscle weakness, elevated levels of creatine kinase (CK), hypocalcemia, exercise intolerance, and the presence of tubular aggregates (TAs). Here, we generated a knock-in mouse model based on a human gain-of-function mutation which results in a severe, early-onset form of TAM, by inducing a glycine-to-serine point mutation in the ORAI1 pore (Orai1G100S/+ or GS mice). By 8 months of age, GS mice exhibited significant muscle weakness, exercise intolerance, elevated CK levels, hypocalcemia, and robust TA presence. Unexpectedly, constitutive Ca2+ entry in mutant mice was observed in muscle only during early development and was abolished in adult skeletal muscle, partly due to reduced ORAI1 expression. Consistent with proteomic results, significant mitochondrial damage and dysfunction was observed in skeletal muscle of GS mice. Thus, GS mice represent a powerful model for investigation of the pathophysiological mechanisms that underlie key TAM symptoms, as well as those compensatory responses that limit the damaging effects of uncontrolled ORAI1-mediated Ca2+ influx.","PeriodicalId":501009,"journal":{"name":"The EMBO Journal","volume":"106 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure of a step II catalytically activated spliceosome from Chlamydomonas reinhardtii. 莱茵衣藻第二步催化活化剪接体的结构。

The EMBO Journal Pub Date : 2024-10-16 DOI: 10.1038/s44318-024-00274-3

Yichen Lu,Ke Liang,Xiechao Zhan

引用次数: 0

The Nedd4L ubiquitin ligase is activated by FCHO2-generated membrane curvature. Nedd4L 泛素连接酶由 FCHO2 产生的膜曲率激活。

The EMBO Journal Pub Date : 2024-10-14 DOI: 10.1038/s44318-024-00268-1

Yasuhisa Sakamoto,Akiyoshi Uezu,Koji Kikuchi,Jangmi Kang,Eiko Fujii,Toshiro Moroishi,Shiro Suetsugu,Hiroyuki Nakanishi

{"title":"The Nedd4L ubiquitin ligase is activated by FCHO2-generated membrane curvature.","authors":"Yasuhisa Sakamoto,Akiyoshi Uezu,Koji Kikuchi,Jangmi Kang,Eiko Fujii,Toshiro Moroishi,Shiro Suetsugu,Hiroyuki Nakanishi","doi":"10.1038/s44318-024-00268-1","DOIUrl":"https://doi.org/10.1038/s44318-024-00268-1","url":null,"abstract":"The C2-WW-HECT domain ubiquitin ligase Nedd4L regulates membrane sorting during endocytosis through the ubiquitination of cargo molecules such as the epithelial sodium channel (ENaC). Nedd4L is catalytically autoinhibited by an intramolecular interaction between its C2 and HECT domains, but the protein's activation mechanism is poorly understood. Here, we show that Nedd4L activation is linked to membrane shape by FCHO2, a Bin-Amphiphysin-Rsv (BAR) domain protein that regulates endocytosis. FCHO2 was required for the Nedd4L-mediated ubiquitination and endocytosis of ENaC, with Nedd4L co-localizing with FCHO2 at clathrin-coated pits. In cells, Nedd4L was specifically recruited to, and activated by, the FCHO2 BAR domain. Furthermore, we reconstituted FCHO2-induced recruitment and activation of Nedd4L in vitro. Both the recruitment and activation were mediated by membrane curvature rather than protein-protein interactions. The Nedd4L C2 domain recognized a specific degree of membrane curvature that was generated by the FCHO2 BAR domain, with this curvature directly activating Nedd4L by relieving its autoinhibition. Thus, we show for the first time a specific function (i.e., recruitment and activation of an enzyme regulating cargo sorting) of membrane curvature by a BAR domain protein.","PeriodicalId":501009,"journal":{"name":"The EMBO Journal","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142439545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dynamics and functional impact of tRNA repertoires during early embryogenesis in zebrafish. 斑马鱼早期胚胎发育过程中 tRNA 重排的动态和功能影响。

The EMBO Journal Pub Date : 2024-10-14 DOI: 10.1038/s44318-024-00265-4

Madalena M Reimão-Pinto,Andrew Behrens,Sergio Forcelloni,Klemens Fröhlich,Selay Kaya,Danny D Nedialkova

{"title":"The dynamics and functional impact of tRNA repertoires during early embryogenesis in zebrafish.","authors":"Madalena M Reimão-Pinto,Andrew Behrens,Sergio Forcelloni,Klemens Fröhlich,Selay Kaya,Danny D Nedialkova","doi":"10.1038/s44318-024-00265-4","DOIUrl":"https://doi.org/10.1038/s44318-024-00265-4","url":null,"abstract":"Embryogenesis entails dramatic shifts in mRNA translation and turnover that reprogram gene expression during cellular proliferation and differentiation. Codon identity modulates mRNA stability during early vertebrate embryogenesis, but how the composition of tRNA pools is matched to translational demand is unknown. By quantitative profiling of tRNA repertoires in zebrafish embryos during the maternal-to-zygotic transition, we show that zygotic tRNA repertoires are established after the onset of gastrulation, succeeding the major wave of zygotic mRNA transcription. Maternal and zygotic tRNA pools are distinct, but their reprogramming does not result in a better match to the codon content of the zygotic transcriptome. Instead, we find that an increase in global translation at gastrulation sensitizes decoding rates to tRNA supply, thus destabilizing maternal mRNAs enriched in slowly translated codons. Translational activation and zygotic tRNA expression temporally coincide with an increase of TORC1 activity at gastrulation, which phosphorylates and inactivates the RNA polymerase III repressor Maf1a/b. Our data indicate that a switch in global translation, rather than tRNA reprogramming, determines the onset of codon-dependent maternal mRNA decay during zebrafish embryogenesis.","PeriodicalId":501009,"journal":{"name":"The EMBO Journal","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142439546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRPC5 controls the adrenaline-mediated counter regulation of hypoglycemia. TRPC5 控制肾上腺素介导的低血糖反调节。

The EMBO Journal Pub Date : 2024-10-07 DOI: 10.1038/s44318-024-00231-0

Jenny Bröker-Lai,José Rego Terol,Christin Richter,Ilka Mathar,Angela Wirth,Stefan Kopf,Ana Moreno-Pérez,Michael Büttner,Linette Liqi Tan,Mazen Makke,Gernot Poschet,Julia Hermann,Volodymyr Tsvilovskyy,Uwe Haberkorn,Philipp Wartenberg,Sebastian Susperreguy,Michael Berlin,Roger Ottenheijm,Koenraad Philippaert,Moya Wu,Tobias Wiedemann,Stephan Herzig,Anouar Belkacemi,Rebecca T Levinson,Nitin Agarwal,Juan E Camacho Londoño,Bert Klebl,Klaus Dinkel,Frank Zufall,Peter Nussbaumer,Ulrich Boehm,Rüdiger Hell,Peter Nawroth,Lutz Birnbaumer,Trese Leinders-Zufall,Rohini Kuner,Markus Zorn,Dieter Bruns,Yvonne Schwarz,Marc Freichel

{"title":"TRPC5 controls the adrenaline-mediated counter regulation of hypoglycemia.","authors":"Jenny Bröker-Lai,José Rego Terol,Christin Richter,Ilka Mathar,Angela Wirth,Stefan Kopf,Ana Moreno-Pérez,Michael Büttner,Linette Liqi Tan,Mazen Makke,Gernot Poschet,Julia Hermann,Volodymyr Tsvilovskyy,Uwe Haberkorn,Philipp Wartenberg,Sebastian Susperreguy,Michael Berlin,Roger Ottenheijm,Koenraad Philippaert,Moya Wu,Tobias Wiedemann,Stephan Herzig,Anouar Belkacemi,Rebecca T Levinson,Nitin Agarwal,Juan E Camacho Londoño,Bert Klebl,Klaus Dinkel,Frank Zufall,Peter Nussbaumer,Ulrich Boehm,Rüdiger Hell,Peter Nawroth,Lutz Birnbaumer,Trese Leinders-Zufall,Rohini Kuner,Markus Zorn,Dieter Bruns,Yvonne Schwarz,Marc Freichel","doi":"10.1038/s44318-024-00231-0","DOIUrl":"https://doi.org/10.1038/s44318-024-00231-0","url":null,"abstract":"Hypoglycemia triggers autonomic and endocrine counter-regulatory responses to restore glucose homeostasis, a response that is impaired in patients with diabetes and its long-term complication hypoglycemia-associated autonomic failure (HAAF). We show that insulin-evoked hypoglycemia is severely aggravated in mice lacking the cation channel proteins TRPC1, TRPC4, TRPC5, and TRPC6, which cannot be explained by alterations in glucagon or glucocorticoid action. By using various TRPC compound knockout mouse lines, we pinpointed the failure in sympathetic counter-regulation to the lack of the TRPC5 channel subtype in adrenal chromaffin cells, which prevents proper adrenaline rise in blood plasma. Using electrophysiological analyses, we delineate a previously unknown signaling pathway in which stimulation of PAC1 or muscarinic receptors activates TRPC5 channels in a phospholipase-C-dependent manner to induce sustained adrenaline secretion as a crucial step in the sympathetic counter response to insulin-induced hypoglycemia. By comparing metabolites in the plasma, we identified reduced taurine levels after hypoglycemia induction as a commonality in TRPC5-deficient mice and HAAF patients.","PeriodicalId":501009,"journal":{"name":"The EMBO Journal","volume":"226 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142385409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

O-GlcNAcylation of circadian clock protein Bmal1 impairs cognitive function in diabetic mice. 昼夜节律钟蛋白 Bmal1 的 O-GlcNAcylation 会损害糖尿病小鼠的认知功能。

The EMBO Journal Pub Date : 2024-10-07 DOI: 10.1038/s44318-024-00263-6

Ya Hui,Yuanmei Zhong,Liuyu Kuang,Jingxi Xu,Yuqi Hao,Jingxue Cao,Tianpeng Zheng

引用次数: 0

Alternative splicing of PBRM1 mediates resistance to PD-1 blockade therapy in renal cancer. PBRM1的替代剪接介导了肾癌患者对PD-1阻断疗法的耐受性。

The EMBO Journal Pub Date : 2024-10-07 DOI: 10.1038/s44318-024-00262-7

Namjoon Cho,Seung-Yeon Kim,Sung-Gwon Lee,Chungoo Park,Sunkyung Choi,Eun-Mi Kim,Kee K Kim

引用次数: 0

PIWI-interacting RNAs: who, what, when, where, why, and how. 与 PIWI 相互作用的 RNA：谁、什么、何时、何地、为何以及如何作用。

The EMBO Journal Pub Date : 2024-09-26 DOI: 10.1038/s44318-024-00253-8

Astrid D Haase,Rene F Ketting,Eric C Lai,Ronald P van Rij,Mikiko Siomi,Petr Svoboda,Josien C van Wolfswinkel,Pei-Hsuan Wu

引用次数: 0

CEP192 localises mitotic Aurora-A activity by priming its interaction with TPX2. CEP192 通过启动有丝分裂 Aurora-A 与 TPX2 的相互作用，定位 Aurora-A 的活性。

The EMBO Journal Pub Date : 2024-09-26 DOI: 10.1038/s44318-024-00240-z

James Holder,Jennifer A Miles,Matthew Batchelor,Harrison Popple,Martin Walko,Wayland Yeung,Natarajan Kannan,Andrew J Wilson,Richard Bayliss,Fanni Gergely

{"title":"CEP192 localises mitotic Aurora-A activity by priming its interaction with TPX2.","authors":"James Holder,Jennifer A Miles,Matthew Batchelor,Harrison Popple,Martin Walko,Wayland Yeung,Natarajan Kannan,Andrew J Wilson,Richard Bayliss,Fanni Gergely","doi":"10.1038/s44318-024-00240-z","DOIUrl":"https://doi.org/10.1038/s44318-024-00240-z","url":null,"abstract":"Aurora-A is an essential cell-cycle kinase with critical roles in mitotic entry and spindle dynamics. These functions require binding partners such as CEP192 and TPX2, which modulate both kinase activity and localisation of Aurora-A. Here we investigate the structure and role of the centrosomal Aurora-A:CEP192 complex in the wider molecular network. We find that CEP192 wraps around Aurora-A, occupies the binding sites for mitotic spindle-associated partners, and thus competes with them. Comparison of two different Aurora-A conformations reveals how CEP192 modifies kinase activity through the site used for TPX2-mediated activation. Deleting the Aurora-A-binding interface in CEP192 prevents centrosomal accumulation of Aurora-A, curtails its activation-loop phosphorylation, and reduces spindle-bound TPX2:Aurora-A complexes, resulting in error-prone mitosis. Thus, by supplying the pool of phosphorylated Aurora-A necessary for TPX2 binding, CEP192:Aurora-A complexes regulate spindle function. We propose an evolutionarily conserved spatial hierarchy, which protects genome integrity through fine-tuning and correctly localising Aurora-A activity.","PeriodicalId":501009,"journal":{"name":"The EMBO Journal","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142328986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA degradation triggered by decapping is largely independent of initial deadenylation. 脱帽引发的 RNA 降解在很大程度上与最初的去淀粉化无关。

The EMBO Journal Pub Date : 2024-09-25 DOI: 10.1038/s44318-024-00250-x

Léna Audebert,Frank Feuerbach,Mostafa Zedan,Alexandra P Schürch,Laurence Decourty,Abdelkader Namane,Emmanuelle Permal,Karsten Weis,Gwenaël Badis,Cosmin Saveanu

{"title":"RNA degradation triggered by decapping is largely independent of initial deadenylation.","authors":"Léna Audebert,Frank Feuerbach,Mostafa Zedan,Alexandra P Schürch,Laurence Decourty,Abdelkader Namane,Emmanuelle Permal,Karsten Weis,Gwenaël Badis,Cosmin Saveanu","doi":"10.1038/s44318-024-00250-x","DOIUrl":"https://doi.org/10.1038/s44318-024-00250-x","url":null,"abstract":"RNA stability, important for eukaryotic gene expression, is thought to depend on deadenylation rates, with shortened poly(A) tails triggering decapping and 5' to 3' degradation. In contrast to this view, recent large-scale studies indicate that the most unstable mRNAs have, on average, long poly(A) tails. To clarify the role of deadenylation in mRNA decay, we first modeled mRNA poly(A) tail kinetics and mRNA stability in yeast. Independent of deadenylation rates, differences in mRNA decapping rates alone were sufficient to explain current large-scale results. To test the hypothesis that deadenylation and decapping are uncoupled, we used rapid depletion of decapping and deadenylation enzymes and measured changes in mRNA levels, poly(A) length and stability, both transcriptome-wide and with individual reporters. These experiments revealed that perturbations in poly(A) tail length did not correlate with variations in mRNA stability. Thus, while deadenylation may be critical for specific regulatory mechanisms, our results suggest that for most yeast mRNAs, it is not critical for mRNA decapping and degradation.","PeriodicalId":501009,"journal":{"name":"The EMBO Journal","volume":"217 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142325035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0