Signa Vitae最新文献_第2页

Protopine regulates oxidative stress, apoptosis and autophagy in H9C2 cardiomyocytes under hypoxic conditions Protopine调节缺氧条件下H9C2心肌细胞的氧化应激、凋亡和自噬

4区医学

Signa Vitae Pub Date : 2024-01-01 DOI: 10.22514/sv.2024.132

{"title":"Protopine regulates oxidative stress, apoptosis and autophagy in H9C2 cardiomyocytes under hypoxic conditions","authors":"","doi":"10.22514/sv.2024.132","DOIUrl":"https://doi.org/10.22514/sv.2024.132","url":null,"abstract":"The heart is highly sensitive to oxygen deprivation, and hypoxia can lead to cardiomyocyte damage and subsequent cardiac dysfunction. Protopine Pro), an isoquinoline alkaloid derived from various herbs, exhibits diverse biological activities. However, the protective mechanisms of Pro against hypoxia-induced cardiomyocyte damage are not well understood. In this study, Pro was observed to stimulate cell proliferation in H9C2 cardiomyocytes under hypoxia/reoxygenation (H/R) conditions and mitigate apoptosis typically associated with H/R exposure. Despite a notable increase in oxidative stress following H/R treatment, Pro treatment effectively reduced this effect. Additionally, Pro was found to enhance autophagy in H/R-treated H9C2 cells, as evidenced by higher microtubule-associated protein 1 light chain 3 (LC3)-II/LC3-I ratios and increased LC3B fluorescence intensity. The activation of the phosphatase and tensin homolog deleted on chromosome ten (PTEN)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway by Pro was also demonstrated. Overall, Pro influences oxidative stress, apoptosis, and autophagy in H9C2 cardiomyocytes under H/R conditions by activating the PTEN/PI3K/Akt pathway, suggesting it as a promising agent to mitigate hypoxia-induced cardiomyocyte damage.","PeriodicalId":49522,"journal":{"name":"Signa Vitae","volume":"20 10","pages":"113-113"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147917562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tussilagone inhibits inflammation and oxidative stress to alleviate acute pancreatitis Tussilagone抑制炎症和氧化应激，减轻急性胰腺炎

4区医学

Signa Vitae Pub Date : 2024-01-01 DOI: 10.22514/sv.2024.009

Chuan Zhang, Mingrong Xie, Yanmei Wang, Tingyong Han

{"title":"Tussilagone inhibits inflammation and oxidative stress to alleviate acute pancreatitis","authors":"Chuan Zhang, Mingrong Xie, Yanmei Wang, Tingyong Han","doi":"10.22514/sv.2024.009","DOIUrl":"https://doi.org/10.22514/sv.2024.009","url":null,"abstract":"Acute pancreatitis (AP) is a clinical emergency characterized by elevated levels of inflammation and oxidative stress, urging the need for the development of new and more efficacious treatments. Tussilagone (TSL), a compound from Tussilago farfara flower buds, is known to exhibit diverse properties, including anti-inflammatory activity, but its precise role in AP remains unclear. In this study, we investigated TSL’s impact on the progression of AP. To establish AP animal models, mice were intraperitoneally administered hyranolin. Our findings demonstrate that TSL effectively suppresses the progression of AP in these mice and decreases inflammation in their pancreatic tissues, as evidenced by reduced secretion of inflammatory cytokines such as interleukin (IL)-1β, Tumor necrosis factor (TNF)-α and IL-6 (p < 0.05). Notably, TSL also mitigates neutrophil infiltration into the pancreatic tissues of AP-afflicted mice and alleviates oxidative stress in AP mice, as confirmed by the measurements of Superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), and myeloperoxidase (MPO) (p < 0.05). Remarkably, TSL exerts its inhibitory effects on inflammation and oxidative stress by blocking the nuclear factor (NF)-κB pathway and activating the NF-E2-related factor 2 (Nrf2) pathway. In conclusion, TSL can mitigate inflammation and oxidative stress, offering potential as a promising therapeutic agent for AP.","PeriodicalId":49522,"journal":{"name":"Signa Vitae","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://oss.signavitae.com/mre-signavitae/article/20240108-256/pdf/SV2023111401.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147884451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In vitro effects of Ganoderic acid A on NF-κB-mediated inflammatory response in caerulein-stimulated pancreatic acinar cells 灵芝酸A对小蛋白刺激胰腺腺泡细胞NF-κ b介导炎症反应的体外影响

4区医学

Signa Vitae Pub Date : 2024-01-01 DOI: 10.22514/sv.2024.075

{"title":"In vitro effects of Ganoderic acid A on NF-κB-mediated inflammatory response in caerulein-stimulated pancreatic acinar cells","authors":"","doi":"10.22514/sv.2024.075","DOIUrl":"https://doi.org/10.22514/sv.2024.075","url":null,"abstract":"This study aimed to investigate Ganoderic acid A’s (GAA) possible effects on inflammation and oxidative stress of caerulein-stimulated pancreatic acinar cells and uncover its effects on acute pancreatitis (AP). A cell model of AP was constructed by treating pancreatic acinar AR42J cells with caerulein. Cell counting kit-8 (CCK-8) assay was used to measure cell viability, while Enzyme-Linked Immunosorbent Assay (ELISA) and 2′,7′-Dichlorofluorescein (DCF) fluorescence assays were used to measure inflammation and oxidative stress. Western blot analysis was used to investigate Nuclear Factor-kappa B (NF-κB) signaling pathway inhibition, focusing on p65 and NF-kappa-B inhibitor alpha (IκBα) phosphorylation states. GAA significantly enhanced cell viability in caerulein-stimulated pancreatic acinar AR42J cells. It also significantly reduced AR42J cells’ inflammatory response. Furthermore, GAA treatment mitigated oxidative stress by decreasing Reactive oxygen species (ROS) production. Lastly, GAA inhibited the NF-κB pathway, as evidenced by decreased p65 and IκBα phosphorylation. By inhibiting NF-B-mediated inflammation, GAA attenuated caerulein-induced AP.","PeriodicalId":49522,"journal":{"name":"Signa Vitae","volume":"20 6","pages":"93-93"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147893098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Cirsilineol reduces inflammation and apoptosis in an in vitro model of acute pancreatitis 在体外急性胰腺炎模型中，cisilineol减少炎症和细胞凋亡

4区医学

Signa Vitae Pub Date : 2024-01-01 DOI: 10.22514/sv.2024.111

引用次数: 2

Fargesin promotes LPS-alleviated microglial M2 polarization and relieves neuropathic pain Fargesin促进lps减轻的小胶质细胞M2极化，减轻神经性疼痛

4区医学

Signa Vitae Pub Date : 2024-01-01 DOI: 10.22514/sv.2024.103

Chenglong Wu, Ling Zhou, Li Wang, Lu Liu

引用次数: 3

The role of Macklin effect in management of ARDS: beyond spontaneous pneumomediastinum 麦克林效应在ARDS治疗中的作用：自发性纵隔气肿

4区医学

Signa Vitae Pub Date : 2024-01-01 DOI: 10.22514/sv.2024.137

{"title":"The role of Macklin effect in management of ARDS: beyond spontaneous pneumomediastinum","authors":"","doi":"10.22514/sv.2024.137","DOIUrl":"https://doi.org/10.22514/sv.2024.137","url":null,"abstract":"Barotrauma (including pneumomediastinum, pneumothorax or subcutaneous emphysema) is a frequent complication of patients with acute respiratory distress syndrome (ARDS) and is associated with worse outcome. Furthermore, some Authors hypothesize that pneumomediastinum could be a sign of ongoing patient self-inflicted lung injury (P-SILI) in patients with respiratory failure receiving non-invasive respiratory support. It has been recently found that a well-known radiological sign, the Macklin effect (or pulmonary interstitial emphysema), could be a powerful predictor of subsequent development of barotrauma in patients with ARDS (sensitivity = 89.2% (95% confidence interval (CI): 74.6 to 96.9); specificity = 95.6% (95% CI: 90.6 to 98.4)). Of note, Macklin effect is visible on chest computed tomography (CT) scan 8–12 days before overt barotrauma. Furthermore, patients with Macklin effect not currently receiving invasive ventilation have a high risk of subsequent intubation. Accordingly, it could be hypothesized that Macklin effect could be a marker of lung fragility, disease severity, and P-SILI in patients with ARDS. Therefore, detection of Macklin effect on chest CT scan could be used to stratify baseline risk of patients with ARDS, select which patients should be evaluated for alternative management algorithms, including advanced respiratory monitoring, ultraprotective ventilation, or institution of extracorporeal support without invasive ventilation.","PeriodicalId":49522,"journal":{"name":"Signa Vitae","volume":"20 11","pages":"10-10"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147894234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Alisol A attenuates Sevoflurane-induced hippocampal neuron injury via targeting SIRT3 Alisol A通过靶向SIRT3减轻七氟醚诱导的海马神经元损伤

4区医学

Signa Vitae Pub Date : 2024-01-01 DOI: 10.22514/sv.2024.099

Jing Hu, Tianyin Liu, Min Zeng

{"title":"Alisol A attenuates Sevoflurane-induced hippocampal neuron injury via targeting SIRT3","authors":"Jing Hu, Tianyin Liu, Min Zeng","doi":"10.22514/sv.2024.099","DOIUrl":"https://doi.org/10.22514/sv.2024.099","url":null,"abstract":"To investigate the effects of Alisol A on Sevoflurane (Sev)-induced neuroinflammation in hippocampal HT22 cells and explores the underlying mechanisms involving Sirtuin 3 (SIRT3) activation. Neuroinflammation was induced through the exposure of HT22 cells were exposed to 4% for 6 h. We evaluated cell viability through cell counting kit-8 (CCK-8) assays, apoptosis and ferroptosis through flow cytometry (FCM) and immunoblot assays. Alisol A effects at different concentrations (0, 2.5, 5, 10 and 20 µM) on Sev-induced cell damage were examined. Additionally, using the SIRT3 inhibitor 3-(Benzylamino)-3-Methylbutanoic Acid (3-TYP), SIRT3’s role in mediating these effects was investigated. Alisol A significantly increased Sev-induced HT22 cell viability and inhibited Sev-induced apoptosis and ferroptosis. Alisol A treatment decreased levels of apoptotic markers (Bcl-2 associated X protein (Bax), cleaved caspase-3, cleaved poly ADP-ribose polymerase (PARP)) and ferroptosis markers (MDA, GSH), while increasing expressions of SIRT3 and ferroptosis inhibitors (glutathione peroxidase 4 (GPX4), Solute Carrier Family 7 Member 11 (SLC7A11)). Alisol A’s protective effects were reversed by 3-TYP, confirming SIRT3 activation involvement. Alisol A mitigates Sev-induced neuroinflammation and cell damage in HT22 cells by activating SIRT3. Alisol A may therefore be a promising drug for treating Sev-induced neurotoxicity.","PeriodicalId":49522,"journal":{"name":"Signa Vitae","volume":"20 8","pages":"61-61"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://oss.signavitae.com/mre-signavitae/article/20240808-405/pdf/SV2024053101.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147902830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Artesunate inhibits ROS production and ameliorates mitochondrial damage through the SIRT1/FOXO3a/MnSOD pathway to alleviate heart failure 青蒿琥酯通过SIRT1/FOXO3a/MnSOD通路抑制ROS生成，改善线粒体损伤，减轻心力衰竭

4区医学

Signa Vitae Pub Date : 2024-01-01 DOI: 10.22514/sv.2024.116

引用次数: 1

The effects of perioperative low-dose magnesium sulfate infusion on postoperative pain in lumbar surgery 围手术期小剂量硫酸镁输液对腰椎术后疼痛的影响

4区医学

Signa Vitae Pub Date : 2023-01-01 DOI: 10.22514/sv.2023.097

{"title":"The effects of perioperative low-dose magnesium sulfate infusion on postoperative pain in lumbar surgery","authors":"","doi":"10.22514/sv.2023.097","DOIUrl":"https://doi.org/10.22514/sv.2023.097","url":null,"abstract":"This paper aimed at evaluating the effect of low-dose perioperative magnesium sulfate (MgSO4) on postoperative pain in lumbar surgery. Sixty adult patients were included in this randomized controlled double-blind study. After intubation, an infusion of MgSO4 (10 mg/kg/h) and isotonic saline 0.9% (10 mg/kg/h) were administered in groups M and C, respectively. Heart rate and mean arterial pressure were recorded before and during surgery. Serum magnesium levels were recorded before and after the surgery. Perioperative remifentanil consumption and extubation time were recorded. In the post-anesthesia care room (PACU), Agitation-sedation with Richmond Agitation-Sedation Scale (RASS), the pain was evaluated with Visual Analogue Scale (VAS) and recorded at the baseline, at 5th, 10th, 15th and 30th minutes. Patients were transferred to surgical ward with intravenous patient-controlled analgesia (PCA) for postoperative analgesia and received diclofenac sodium for rescue analgesia if VAS >4. Postoperative VAS scores at 2nd, 4th, 6th and 24th hours, opioid consumption after 4 and 24 hours and rescue analgesic consumption for postoperative 24 h were recorded. In the PACU, RASS scores were statistically lower in group M than in group C (p = 0.001), and VAS scores were statistically lower at all measurement times in group M than in group C. In the surgical ward, VAS Scores were statistically lower in Group M at the 6th and 24th hours than in group C (p = 0.015, p = 0.009, respectively). The need for post-operative rescue analgesic was statistically lower in Group M (p < 0.001). The side effect incidence of both groups was similar. Our findings suggest that perioperative low-dose (10 mg/kg/h) MgSO4 infusion reduces early post-op agitation, VAS scores and the need for analgesics up to 24 hours postoperatively. Low dose MgSO4 infusion can be effectively applied for pain management in patients undergoing lumbar surgery.","PeriodicalId":49522,"journal":{"name":"Signa Vitae","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135106285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of volume-controlled and pressure-controlled ventilation modes on cerebral oximetry in laparoscopic cholecystectomy: a randomized controlled trial 容量控制和压力控制通气模式对腹腔镜胆囊切除术脑血氧测定的影响:一项随机对照试验

4区医学

Signa Vitae Pub Date : 2023-01-01 DOI: 10.22514/sv.2023.098

{"title":"Effect of volume-controlled and pressure-controlled ventilation modes on cerebral oximetry in laparoscopic cholecystectomy: a randomized controlled trial","authors":"","doi":"10.22514/sv.2023.098","DOIUrl":"https://doi.org/10.22514/sv.2023.098","url":null,"abstract":"This study aimed to compare the effect of volume-controlled ventilation (VCV) and pressure-controlled ventilation (PCV) modes on cerebral oximetry during laparoscopic cholecystectomy using near-infrared spectroscopy (NIRS). Seventy patients who underwent elective laparoscopic cholecystectomy were randomized to receive either VCV (group V) or PCV (group P). Demographic and operative data (anesthesia, surgery and insufflation durations) were recorded. The primary outcome was the NIRS value, while the secondary outcomes were peripheral oxygen saturation (SpO2), blood gas parameters and peak and plateau pressures in mechanical ventilation. Measurements were conducted at the start of anesthesia (T0), end of intubation (T1), 5 min after insufflation (T2), just before desufflation (T3), and 5 min after desufflation (T4). Both groups were comparable in terms of age, sex, body mass index, intraoperative time, anesthesia and insufflation durations. The average NIRS right T1–T2–T3 and left T2–T3 values were significantly higher in group P than in group V (p = 0.030, p = 0.001, p = 0.001, p = 0.006 and p = 0.002 respectively). In contrast, the mean peak and mean plateau pressures in group P at T1, T2 and T4 were significantly lower than those in group V (p = 0.003, p = 0.001, p < 0.001, p = 0.011, p = 0.001 and p < 0.001 respectively).The PCV mode allows better cerebral oxygenation than VCV while maintaining lower peak pressure and plateau pressures.","PeriodicalId":49522,"journal":{"name":"Signa Vitae","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135107523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0