Biotechnology for Biofuels最新文献

{"title":"Characterization of microalgal β-carotene and astaxanthin: exploring their health-promoting properties under the effect of salinity and light intensity","authors":"Aditi,&nbsp;Rupesh Bhardwaj,&nbsp;Ankush Yadav,&nbsp;Prashant Swapnil,&nbsp;Mukesh Meena","doi":"10.1186/s13068-025-02612-x","DOIUrl":"10.1186/s13068-025-02612-x","url":null,"abstract":"<div><p>Microalgae are promising sources of valuable carotenoids like β-carotene and astaxanthin with numerous health benefits. This review summarizes recent studies on producing these carotenoids in microalgae under different salinity and light-intensity conditions, which are key factors influencing their biosynthesis. The carotenoid biosynthesis pathways in microalgae, involving the methylerythritol phosphate pathway in chloroplasts, are described in detail. The effects of high salinity and light stress on stimulating astaxanthin accumulation in species like <i>Haematococcus pluvialis</i> and <i>Chromochloris zofingiensis</i> and their synergistic impact are discussed. Similarly, the review covers how high light and salinity induce β-carotene production in <i>Dunaliella salina</i> and other microalgae. The diverse health-promoting properties of astaxanthin and β-carotene, such as their antioxidant, antiinflammatory, and anticancer activities, are highlighted. Strategies to improve carotenoid yields in microalgae through environmental stresses, two-stage cultivation, genetic engineering, and metabolic engineering approaches are evaluated. Overall, this review highlights advancements in β-carotene and astaxanthin production reporting the different microalgal capability to produce carotenoids under different stress level like 31.5% increase in β-carotene accumulation in <i>Dunaliella salina</i> and astaxanthin productivity reaching 18.1 mg/L/day in <i>Haematococcus lacustris</i>. It also explores novel biotechnological strategies, including CRISPR–Cas9, for enhancing carotenoid yield.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-025-02612-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactic whey as a potential feedstock for exopolysaccharide production by microalgae strain Neochloris oleoabundans UTEX 1185","authors":"Daniel Moisés Paredes-Molina,&nbsp;Miguel A. Cervantes-López,&nbsp;Domancar Orona-Tamayo,&nbsp;Nancy E. Lozoya-Pérez,&nbsp;Flora I. Beltrán-Ramírez,&nbsp;Juan Vázquez-Martínez,&nbsp;Karla L. Macias-Sánchez,&nbsp;Sergio Alonso-Romero,&nbsp;Elizabeth Quintana-Rodríguez","doi":"10.1186/s13068-024-02595-1","DOIUrl":"10.1186/s13068-024-02595-1","url":null,"abstract":"<div><h3>Background</h3><p>Lactic whey, a significant agro-industrial byproduct, poses environmental risks due to its chemical composition. Despite various valorization efforts, effective utilization remains a challenge. This study explores the potential of <i>Neochloris oleoabundans</i>, a microalgae known for its metabolic versatility and resilience to adverse conditions, to produce exopolysaccharides (EPS) using lactic whey as a substrate. We compared EPS production from lactose, the primary sugar in whey, with whole lactic whey. Characterization of the EPS was performed using Fourier transform infrared spectroscopy (FT-IR) and gas chromatography–mass spectrometry (GC–MS), while morphological analysis was conducted via scanning electron microscopy (SEM). This research aims to assess the feasibility of converting lactic whey into valuable EPS, providing a sustainable approach to managing this agro-industrial waste.</p><h3>Results</h3><p>Lactic whey has produced the highest EPS and the FT-IR spectra revealed structural variations in the monomers which compose these polymers. Galactose and glucose were shown to be the primary monomers, according to GC–MS EPS analysis. SEM revealed a homogenous matrix and <i>N. oleoabundans</i>'s bioflocculant characteristics.</p><h3>Conclusions</h3><p>Microalgae <i>N. oleoabundans</i> can produce EPS using lactic whey as feedstock and it has the potential to be employed as a wastewater treatment.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-024-02595-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Newly isolated halotolerant Gordonia terrae S-LD serves as a microbial cell factory for the bioconversion of used soybean oil into polyhydroxybutyrate","authors":"Song Xu,&nbsp;Ruiqin Han,&nbsp;Lidan Tao,&nbsp;Zhipeng Zhang,&nbsp;Junfei Gao,&nbsp;Xinyuan Wang,&nbsp;Wei Zhao,&nbsp;Xiaoxia Zhang,&nbsp;Zhiyong Huang","doi":"10.1186/s13068-025-02613-w","DOIUrl":"10.1186/s13068-025-02613-w","url":null,"abstract":"<div><p>Polyhydroxybutyrate (PHB) is a class of biodegradable polymers generally used by prokaryotes as carbon sources and for energy storage. This study explored the feasibility of repurposing used soybean oil (USO) as a cost-effective carbon substrate for the production of PHB by the strain <i>Gordonia terrae</i> S-LD, marking the first report on PHB biosynthesis by this rare actinomycete species. This strain can grow under a broad range of temperatures (25–40 ℃), initial pH values (4–10), and salt concentrations (0–7%). The findings indicate that this strain can synthesize PHB at a level of 2.63 ± 0.6 g/L in a waste-containing medium containing 3% NaCl within a 3 L triangular flask, accounting for 66.97% of the cell dry weight. Furthermore, ¹H NMR, ¹³C NMR, and GC–MS results confirmed that the polymer was PHB. The thermal properties of PHB, including its melting (T_m) and crystallization (T_c) temperatures of 176.34 °C and 56.12 °C respectively, were determined via differential scanning calorimetry analysis. The produced PHB was characterized by a weight-average molecular weight (M_w) of 5.43 × 10⁵ g/mol, a number-average molecular weight (M_n) of 4.00 × 10⁵ g/mol, and a polydispersity index (PDI) of 1.36. In addition, the whole genome was sequenced, and the PHB biosynthetic pathway and quantitative expression of key genes were delineated in the novel isolated strain. In conclusion, this research introduces the first instance of polyhydroxyalkanoate (PHA) production by <i>Gordonia terrae</i> using used soybean oil as the exclusive carbon source, which will enrich strain resources for future PHB biosynthesis.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-025-02613-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143361981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into the role of antioxidant in microbial lignin degradation: Ascorbic acid as a fortifier of lignin-degrading enzymes","authors":"Aipeng Li,&nbsp;Weimin Wang,&nbsp;Shuqi Guo,&nbsp;Changzhi Li,&nbsp;Xinying Wang,&nbsp;Qiang Fei","doi":"10.1186/s13068-025-02614-9","DOIUrl":"10.1186/s13068-025-02614-9","url":null,"abstract":"<div><h3>Background</h3><p>Microbial-driven lignin depolymerization has emerged as a promising approach for lignin degradation. However, this process is hindered by the limited activity of lignin-degrading enzymes. Antioxidants are crucial for maintaining redox homeostasis in living cells, which can impact the efficiency of enzymes. Ascorbic acid (AA) is well-known for its antioxidant properties, while <i>Trametes versicolor</i> is a commonly used lignin-degrading fungus capable of secreting laccase (Lac) and manganese peroxidase (MnP). Thus, AA was selected as model antioxidant and added into the culture medium of <i>T. versicolor</i> to examine the effect of antioxidants on the activity of lignin-degrading enzymes in the fungus.</p><h3>Results</h3><p>The presence of AA resulted in a 4.9-fold increase in the Lac activity and a 3.9-fold increase in the MnP activity, reaching 10736 U/L and 8659 U/L, respectively. This increase in enzyme activity contributed to a higher lignin degradation rate from 17.5% to 35.2%, consistent with observed morphological changes in the lignin structure. Furthermore, the addition of AA led to a reduction in the molecular weights of lignin and an increase in the content of degradation products with lower molecular weight, indicating more thorough degradation of lignin. Proteomics analysis suggested that the enhancement in enzyme activity was more likely to attributed to the reinforcement of AA on oxidative protein folding and transportation, rather than changes in enzyme expression.</p><h3>Conclusions</h3><p>The addition of AA enhanced the performance of enzymes responsible for lignin degradation in terms of enzyme activity, degradation rate, lignin structural change, and product mapping. This study offers a feasible strategy for enhancing the activity of lignin-degrading enzymes in the fungus and provides insights into the role of antioxidant in microbial lignin degradation.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-025-02614-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143361980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’","authors":"Shraddha Maitra,&nbsp;Bruce Dien,&nbsp;Kristen Eilts,&nbsp;Nurzhan Kuanyshev,&nbsp;Yoel R. Cortes-Pena,&nbsp;Yong-Su Jin,&nbsp;Jeremy S. Guest,&nbsp;Vijay Singh","doi":"10.1186/s13068-025-02606-9","DOIUrl":"10.1186/s13068-025-02606-9","url":null,"abstract":"<div><h3>Background</h3><p>Sugarcane plant engineered to accumulate lipids in its vegetative tissue is being developed as a new bioenergy crop. The new crop would be a source of juice, oil, and cellulosic sugars. However, limited tolerance of industrially recognized yeasts towards inhibitors generated during the processing of lignocellulosic biomass to produce fermentable sugars is a major challenge in developing scalable processes for second-generation drop-in fuel production. To this end, hydrolysates generated from engineered sugarcane—‘oilcane’ bagasse contain added phenolics and fatty acids that further restrict the growth of fermenting microorganisms and necessitate nutrient supplementation and/or detoxification of hydrolysate which makes the fermentation process expensive. Herein, we propose a resourceful and economical approach for growing lab and commercial strains of <i>S. cerevisiae</i> on unrefined cellulosic sugars aerobically and fermentatively.</p><h3>Results</h3><p>An equal ratio of hydrolysate and juice was found optimum for growth and fermentation by lab and commercial strains of <i>Saccharomyces cerevisiae</i> engineered for xylose fermentation. The industrial strain grew and fermented efficiently under low aeration conditions having an ethanol titer, yield, specific and volumetric productivities of 46.96 ± 0.19 g/l, 0.51 ± 0.00 g/g, 0.27 ± 0.02 g/g.h and 1.95 ± 0.01 g/l.h, respectively, while the lab strain grew better under higher aeration conditions having the ethanol titer, yield, specific and volumetric productivities of 24.93 ± 0.09, 0.27 ± 0.00 g/g, 0.17 ± 0.00 g/g.h and 1.04 ± 0.00 g/l.h, respectively. Acclimation of cultures in a blended medium significantly improved the performance of the yeast strains.</p><h3>Conclusions</h3><p>The addition of transgenic oilcane juice, which is inedible and rich in amino acids, to the hydrolysate averted the need for expensive nutrient supplementation and detoxification steps of hydrolysate. The approach provides an economical solution to reduce the cost of fermentation at an industrial scale for second-generation drop-in fuel production.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergetic effect of fungal pretreatment and lignin modification on delignification and saccharification: a case study of a natural lignin mutant in mulberry","authors":"James Paul T. Madigal,&nbsp;Masami Terasaki,&nbsp;Masatsugu Takada,&nbsp;Shinya Kajita","doi":"10.1186/s13068-025-02611-y","DOIUrl":"10.1186/s13068-025-02611-y","url":null,"abstract":"<div><h3>Background</h3><p>Fungal pretreatment for partial separation of lignocellulosic components may reduce lignocellulose recalcitrance during the production of biofuels and biochemicals. Quantitative and qualitative modification of plant lignin through genetic engineering or traditional breeding may also reduce the recalcitrance. This study was conducted to examine the effects of combining these two approaches using three white rot fungi and mulberry wood with an altered lignin structure.</p><h3>Results</h3><p>Mulberry wood prepared from homozygotes or heterozygotes with a loss-of-function in the cinnamyl alcohol dehydrogenase gene (<i>CAD</i>) was pretreated with three fungal species. Both heterozygous (<i>CAD</i>/<i>cad</i>) and homozygous (<i>cad</i>/<i>cad</i>, null mutant) mulberry plants were derived from the same parents via backcrossing between Sekizaisou (<i>cad</i>/<i>cad</i>, seed parent), a natural lignin mutant, and its F1 progeny (<i>CAD</i>/<i>cad</i>, pollen parent). Homozygote wood and the isolated lignin exhibited an abnormal color. Lignin in homozygotes without fungal pretreatment exhibited a lower syringyl/guaiacyl ratio, molar mass, and thioacidolysis product yield than those in heterozygotes. Pretreatment with <i>Phanerochaete chrysosporium</i> achieved the highest delignification efficiency with a significant reduction in the cellulose content in both mulberry genotypes. In contrast, <i>Ceriporiopsis subvermispora</i> selectively removed lignin, with a weaker reduction in the cellulose content. The degree of delignification by <i>C. subvermispora</i> was significantly higher in homozygotes than in heterozygotes. <i>Trametes versicolor</i> tended to have a lower delignification capacity and smaller effect of subsequent enzymatic sugar release toward the wood from both genotypes than the other two fungi, making it less suitable for fungal pretreatment. Thioacidolysis assays indicated that cinnamaldehyde β-O-4, a typical subunit in the homozygote lignin, did not contribute to the high degradability of the lignin. The saccharification efficiency tended to be higher in homozygote wood than in heterozygote wood under all fungal pretreatment conditions.</p><h3>Conclusions</h3><p>Although further optimization of various system conditions is required, our findings suggest that CAD deficiency promotes delignification and subsequent enzymatic saccharification and may improve the biorefining efficiency of wood when combined with fungal pretreatment.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11776243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biphasic lipid extraction from microalgae after PEF-treatment reduces the energy demand of the downstream process","authors":"Ioannis Papachristou,&nbsp;Natalja Nazarova,&nbsp;Rüdiger Wüstner,&nbsp;Robin Lina,&nbsp;Wolfgang Frey,&nbsp;Aude Silve","doi":"10.1186/s13068-025-02608-7","DOIUrl":"10.1186/s13068-025-02608-7","url":null,"abstract":"<div><h3>Background</h3><p>The gradual extrusion of water-soluble intracellular components (such as proteins) from microalgae after pulsed electric field (PEF) treatment is a well-documented phenomenon. This could be utilized in biorefinery applications with lipid extraction taking place after such an ‘incubation’ period, i.e., a post-PEF-treatment step during which the biomass is left undisturbed before any further processing. The goal of this work was to further explore how this incubation could improve lipid extraction.</p><h3>Results</h3><p>Experiments were conducted on wet, freshly harvested <i>Auxenochlorella protothecoides</i>, treated with 0.25 or 1.5 MJ/kg_DW and incubated for 24 h. Lipid extraction took place with a monophasic ethanol:hexane:water, 1:0.41:0.04 vol/vol/vol mixture with a 75.6 mL solvent per 1 g of dry biomass ratio. The kinetics of the extraction were studied with samples taken between 10 and 1080 min from fresh and incubated biomass. The yields at 10 min were significantly increased with incubation compared to without (31.2% dry weight compared to 1.81%, respectively). The experimental data were fitted with the Patricelli model where extraction occurs in two steps, a rapid washing of immediate available lipids and a slower diffusion one. During Nile-Red staining of microalgae and microscopy imaging, a shift of emission from both GFP and RFP channels to mostly RFP was observed indicating an increase in the polarity of the environment of Nile-Red. These led to an adaption of a biphasic ethanol:hexane:water 1:6:0.4 vol/vol/vol solvent with 37 mL solvent per 1 g of dry biomass ratio which while ineffective on fresh biomass, achieved a 27% dry weight yield from incubated microalgae. The extraction efficiency in the biphasic route was lower compared to the monophasic (i.e., 69% and 95%, respectively). It was compensated however, by the significant solvent reduction (37 mL to 75.6 mL respectively), in particular the ethanol minimization. For the extraction of 1 L lipids, it was estimated that the energy consumption ratio for the biphasic process was 1.6 compared to 9.9 for monophasic, making clearly the most preferential one.</p><h3>Conclusions</h3><p>This biphasic approach significantly reduces solvent consumption and the respective energy requirement for solvent recovery. Incubation thus could majorly improve the commercialization prospects of the process.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11776281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143061638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of the intracellular β-glucosidase BGL1B from Aspergillus niger in the regulation of lignocellulose-degrading enzymes’ synthesis","authors":"Zhen Zhang,&nbsp;Hua Li,&nbsp;Feiyu Dong,&nbsp;Hui Lin,&nbsp;Yanan Li,&nbsp;Kun Cheng,&nbsp;Hongge Chen","doi":"10.1186/s13068-025-02610-z","DOIUrl":"10.1186/s13068-025-02610-z","url":null,"abstract":"<div><h3>Background</h3><p><i>Aspergillus niger</i> is an important lignocellulose-degrading enzyme-producing strain. Multiple regulatory factors regulate the synthesis of lignocellulose-degrading enzymes in <i>A. niger</i>. We previously found that <i>A. niger</i> possessed an intracellular β-glucosidase BGL1B, and the intracellular localization of BGL1B and its active transglycosylation action prompted us to explore whether BGL1B was involved in the regulation of the synthesis of lignocellulose-degrading enzymes in <i>A. niger</i>.</p><h3>Results</h3><p>In this study, by investigating the production of lignocellulose-degrading enzymes of <i>bgl1B</i> knockout strain (Δ<i>bgl1B</i>) and overexpression strain (OE::<i>bgl1B</i>), it was found that BGL1B exhibited a repressive role on the expression of lignocellulose-degrading enzyme genes through carbon catabolite repression (CCR) way. On the other hand, BGL1B’s transglycosylation products sophorose and laminaribiose were proved to be able to induce the expression of lignocellulose-degrading enzyme genes, which explained why OE::<i>bgl1B</i> showed the same enhanced enzyme activity and gene expression as Δ<i>bgl1B</i> strain compared to the starting strain (WT).</p><h3>Conclusions</h3><p>The present study demonstrates that BGL1B plays dual regulatory roles in the regulation of the synthesis of lignocellulose-degrading enzymes in <i>A. niger</i>: the repressive role caused by BGL1B’s hydrolysis product glucose and the induction role caused by BGL1B’s transglycosylation products sophorose and laminaribiose. This study broadens the understanding of the regulatory network of the synthesis of lignocellulose-degrading enzymes in <i>A. niger</i>. Also, it provides a strategy to create an engineered strain with high production of lignocellulose-degrading enzymes.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shewanella oneidensis and Methanosarcina barkerii augmentation and conductive material effects on long-term anaerobic digestion performance","authors":"Perego Camilla,&nbsp;König Roger,&nbsp;Cuomo Maurizio,&nbsp;Pianta Elisa,&nbsp;Sunny Maye,&nbsp;Loredana Di Maggio,&nbsp;Michel Moser,&nbsp;Fischer Fabian,&nbsp;Principi Pamela","doi":"10.1186/s13068-025-02609-6","DOIUrl":"10.1186/s13068-025-02609-6","url":null,"abstract":"<div><p>This study explores the use of conductive material in scaling up anaerobic digestion for enhanced biogas production. Focusing on Direct Interspecies Electron Transfer (DIET), the research employs a syntrophic DIET-able consortium formed by <i>Shewanella oneidensis</i> and <i>Methanosarcina barkerii</i> in 3.8-L experiments utilizing reticulated vitreous carbon (RVC) as conductive material. In short-term tests with acetate the syntrophic co-culture with RVC resulted in 86% higher maximum velocity of methane production, while in long term with real feed 13% increased rate was observed: the addition of 1.77 (S/m)*m² RVC resulted in a faster methane production of 2.39 mL/gVS*h compared to 2.08 mL/gVS*h of the reference. The experimental conditions of syntrophic inoculum and RVC as conductive material gave a benefit in terms of process rate compared to the reference, considering the inoculum fate, <i>Methanosarcina barkerii</i> was among the dominant taxa at the end of the experiment, while <i>Shewanella oneidensis</i> was outcompeted. Among the methanogenesis production pathways, an increase of hydrogenotrophic methanogenesis has been observed in presence of conductive material. Further research is needed to understand the role of RVC in sulfur compounds production. Utilization of RVC to augment methane production yielded interesting results for real-scale application. As an added carrier, RVC remains unaltered and can be readily recuperated and reused multiple times.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-025-02609-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative metagenomics reveals the metabolic flexibility of coastal prokaryotic microbiomes contributing to lignin degradation","authors":"Qiannan Peng,&nbsp;Lu Lin","doi":"10.1186/s13068-025-02605-w","DOIUrl":"10.1186/s13068-025-02605-w","url":null,"abstract":"<div><p>Coastal wetlands are rich in terrestrial organic carbon. Recent studies suggest that microbial consortia play a role in lignin degradation in coastal wetlands, where lignin turnover rates are likely underestimated. However, the metabolic potentials of these consortia remain elusive. This greatly hinders our understanding of the global carbon cycle and the “bottom-up” design of synthetic consortia to enhance lignin conversion. Here, we developed two groups of lignin degrading consortia, L6 and L18, through the 6- and 18-month in situ lignin enrichments in the coastal East China Sea, respectively. Lignin degradation by L18 was 3.6-fold higher than L6. Using read-based analysis, 16S rRNA amplicon and metagenomic sequencing suggested that these consortia possessed varied taxonomic compositions, yet similar functional traits. Further comparative metagenomic analysis, based on metagenomic assembly, revealed that L18 harbored abundant metagenome-assembled genomes (MAGs) that encoded diverse and unique lignin degradation gene clusters (LDGCs). Importantly, anaerobic MAGs were significantly enriched in L18, highlighting the role of anaerobic lignin degradation. Furthermore, the generalist taxa, which possess metabolic flexibility, increased during the extended enrichment period, indicating the advantage of generalists in adapting to heterogenous resources. This study advances our understanding of the metabolic strategies of coastal prokaryotic consortia and lays a foundation for the design of synthetic communities for sustainable lignocellulose biorefining.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-025-02605-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}