Anatomia Histologia Embryologia最新文献_第9页

Morphological Evaluation of the Hip Joint and Pelvic Limb Bones of the Lowland Tapir (Tapirus terrestris Linnaeus, 1758) Using Radiography and Computed Tomography 低地貘（Tapirus terrestris Linnaeus, 1758）髋关节和骨盆肢体骨的形态学评价。

IF 1 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2026-04-07 DOI: 10.1111/ahe.70110

Heloísa Coppini de Lima, Sheila Canevese Rahal, Jeana Pereira da Silva, Rodrigo Hidalgo Friciello Teixeira, Maria Jaqueline Mamprim, Bruno Critelli Carvalho, Miriam Harumi Tsunemi

{"title":"Morphological Evaluation of the Hip Joint and Pelvic Limb Bones of the Lowland Tapir (Tapirus terrestris Linnaeus, 1758) Using Radiography and Computed Tomography","authors":"Heloísa Coppini de Lima, Sheila Canevese Rahal, Jeana Pereira da Silva, Rodrigo Hidalgo Friciello Teixeira, Maria Jaqueline Mamprim, Bruno Critelli Carvalho, Miriam Harumi Tsunemi","doi":"10.1111/ahe.70110","DOIUrl":"10.1111/ahe.70110","url":null,"abstract":"The study aimed to characterize, through imaging examinations—including radiographs and computed tomography—the hip joint and the bones of the thigh, leg and feet in Lowland tapirs (Tapirus terrestris). Ten pelvic limbs were collected, two from juveniles and the remaining from adults. The femoral head exhibited a rounded morphology with a well-defined fovea capitis and a short, thick neck. Three trochanters were identified, with the greater and third trochanters being particularly prominent. The femoral diaphysis was elongated and straight, and the distal end displayed a globular medial condyle and a flatter lateral condyle. The femoral trochlea consisted of medial and lateral trochlear crests of similar size, separated by a well-defined trochlear groove. The proximal tibia showed slightly concave tibial plateaus, with the lateral condyle being wider and a prominent intercondylar eminence. The fibula was positioned laterally along the entire tibia, with the interosseous space preserved. The talus presented a well-defined trochlea and a head articulating with the central tarsal bone. Tarsal bones I, III and IV were identified, with tarsal IV being elongated. Three metatarsals (II, III and IV) were observed, with metatarsal III relatively more developed. The third digit was larger than both the second and fourth digits. The Norberg angle, femoral head–neck inclination angle, mechanical lateral distal and proximal femoral angles, and anatomical medial proximal and lateral distal tibial angles were similar between the two imaging methods. In conclusion, the imaging examinations were complementary, enabling the characterization of morphological features reflecting the species' functional adaptations for cursorial and semi-aquatic locomotion.","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 3","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anatomical Description of the Main Gyri and Sulci of the Telencephalon of Alouatta belzebul belzebulalouatta端脑主回沟的解剖描述。

IF 1 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2026-04-02 DOI: 10.1111/ahe.70104

Dayane Kelly Sabec Pereira, Fabiano Rodrigues de Melo, Fabiana Cristina Silveira Alves de Melo, Kleber Fernando Pereira, Valcinir Aloisio Scalla Vulcani

{"title":"Anatomical Description of the Main Gyri and Sulci of the Telencephalon of Alouatta belzebul","authors":"Dayane Kelly Sabec Pereira, Fabiano Rodrigues de Melo, Fabiana Cristina Silveira Alves de Melo, Kleber Fernando Pereira, Valcinir Aloisio Scalla Vulcani","doi":"10.1111/ahe.70104","DOIUrl":"10.1111/ahe.70104","url":null,"abstract":"The Alouatta belzebul is a species of howler monkey, of the Atelidae family and genus Alouatta. It is endemic in Brazil and has a separate geographic distribution, with two populations occurring: in the Amazon and in the Atlantic Forest on the coast of Northeast Brazil. The species is classified as ‘vulnerable’, and this is mainly justified by hunting, fragmentation and loss of habitat in tropical forests. Regarding the anatomy of this species, information is scarce and in some aspects such as the brain are non-existent. The objective of this study is to describe the macroscopy of the main grooves and turns of the A. belzebul telencephalon correlating with the degree of encephalisation, the absolute and relative measures of the central groove sinuosity and the straight and sinuous measures of the grooves in different primates. As well as, check the anatomy of the nuclei of the base. Ten A. belzebul brains were used, where the gyrations and grooves were dissected. It was found that the brain surface of A. belzebul presented with lisencephalic characteristics, similar to the findings with several other species of non-human primates and differing from species such as Pan and Homo. In the analysis of the encephalisation index, it was observed that this species is phylogenetically closer to Sapajus and Macaca and more distant than species such as Brachyteles and Callithrix, showing expressive cognition and intelligence. Regarding the slope of the central groove, it was observed that in A. belzebul the upper end is posterior to the lower end, data that approximate with Homo, Papio and Pan and reveal a large frontal lobe when compared to Sapajus libidinosus, showing the maximum brain development in these primates.","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 3","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13044979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147596062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linking Canine Teeth Eruption, Testicular Histology and Testosterone Levels as Indicators of Pubertal Maturation in Male Dogs 链接犬牙的爆发，睾丸组织学和睾丸激素水平作为雄性犬青春期成熟的指标。

IF 1 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2026-03-18 DOI: 10.1111/ahe.70103

Raúl Rebolledo-Martínez, Rodrigo López-Mora, María de la Paz Palacios-Arellano, Miriam Barradas-Moctezuma, Deissy Herrera-Covarrubias, Pedro Paredes-Ramos, Jorge Manzo, Genaro Alfonso Coria-Avila

{"title":"Linking Canine Teeth Eruption, Testicular Histology and Testosterone Levels as Indicators of Pubertal Maturation in Male Dogs","authors":"Raúl Rebolledo-Martínez, Rodrigo López-Mora, María de la Paz Palacios-Arellano, Miriam Barradas-Moctezuma, Deissy Herrera-Covarrubias, Pedro Paredes-Ramos, Jorge Manzo, Genaro Alfonso Coria-Avila","doi":"10.1111/ahe.70103","DOIUrl":"10.1111/ahe.70103","url":null,"abstract":"Puberty in male dogs marks the transition to sexual maturity, characterised by rising testosterone and testicular maturation. While often estimated at 6 months, the absence of birth records frequently complicates age determination. This study evaluated whether permanent canine tooth eruption serves as a reliable, complementary marker for pubertal status alongside chronological age by correlating it with serum testosterone and testicular histology. A sample of 23 male dogs presented for routine gonadectomy were categorised as prepubertal (< 6 months, n = 12) or postpubertal (≥ 6 months, n = 11). Assessments included serum testosterone levels, detailed testicular histology, and the degree of permanent canine eruption. Results revealed that both chronological age at 6 months cutoff and dental eruption (specifically, 2/3 of the permanent canine crown) significantly differentiated prepubertal from postpubertal dogs across key histological parameters. Postpubertal dogs exhibited significantly higher counts of spermatogonia, spermatocytes, and spermatids, alongside larger seminiferous tubule and epithelium areas, and a higher spermatogenic maturation index. However, neither age nor dental criteria were associated with differences in lumen area, Sertoli cell count, or Leydig cell population. While testosterone levels showed a non-significant trend toward being higher in the > 6-month group, they demonstrated a significant positive correlation with the degree of canine eruption. These findings establish that both the conventional six-month age threshold and, more potently, permanent canine eruption are valuable indicators of pubertal status, with dental development providing a superior prediction of underlying endocrine and histological maturation in male dogs.","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 2","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12997144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147476194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spermatogenesis and Morphology of the Male Reproductive Tract of the Turtle Kinosternon scorpioides 沙鳖雄性生殖道的精子发生与形态。

IF 1 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2026-03-16 DOI: 10.1111/ahe.70101

Vinicius Maia Ribeiro de Godoy, Alana Lisleia de Sousa, Mariapaz Dueñas Flórez, Antônio Francisco da Silva Lisboa Neto, Antonio Chaves de Assis Neto

{"title":"Spermatogenesis and Morphology of the Male Reproductive Tract of the Turtle Kinosternon scorpioides","authors":"Vinicius Maia Ribeiro de Godoy, Alana Lisleia de Sousa, Mariapaz Dueñas Flórez, Antônio Francisco da Silva Lisboa Neto, Antonio Chaves de Assis Neto","doi":"10.1111/ahe.70101","DOIUrl":"10.1111/ahe.70101","url":null,"abstract":"The scorpion mud turtle (Kinosternon scorpioides), a freshwater chelonian widely distributed in South and Central America, presents gaps in the microscopic and ultrastructural characterisation of its male reproductive system. This study provides a comprehensive characterisation of the testes, epididymides, and deferent ducts of adult males collected during the rainy reproductive season in the Brazilian Amazon. Morphometric parameters were recorded and showed a positive association between body weight and reproductive tract mass, supporting increased gonadal investment during the breeding period. Tissues were processed for light microscopy (LM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Histological and ultrastructural analyses revealed active spermatogenesis, abundant germ cells in multiple developmental stages, and regionally specialised epithelia along the epididymis and deferent ducts. Spermatozoa exhibited preserved acrosomal, nuclear, mitochondrial and axonemal structures, indicating functional integrity and short-term storage capacity within the male reproductive tract. These findings expand current knowledge of chelonian reproductive biology and provide essential baseline data to support assisted reproductive technologies and conservation strategies for K. scorpioides and related freshwater turtle species.","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 2","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12993256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147469905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating Joint Flexibility and Educational Efficacy: A Comparison of Elnady-Preserved and Plastinated Canine Stifle and Hock Joint Specimens 评估关节灵活性和教育效果：elnady保存和塑化犬膝关节和飞节标本的比较。

IF 1 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2026-03-13 DOI: 10.1111/ahe.70102

Punnarat Viboonchan, Sayamon Srisuwatanasagul, Promporn Raksaseri, Adisorn Adirekthaworn, Thanat Triangkul, Saritvich Panyaboriban, Kongkiat Srisuwatanasagul

{"title":"Evaluating Joint Flexibility and Educational Efficacy: A Comparison of Elnady-Preserved and Plastinated Canine Stifle and Hock Joint Specimens","authors":"Punnarat Viboonchan, Sayamon Srisuwatanasagul, Promporn Raksaseri, Adisorn Adirekthaworn, Thanat Triangkul, Saritvich Panyaboriban, Kongkiat Srisuwatanasagul","doi":"10.1111/ahe.70102","DOIUrl":"10.1111/ahe.70102","url":null,"abstract":"<div>\u0000 \u0000 This study evaluates the comparative efficacy of the Elnady technique and traditional silicone plastination for preserving canine stifle and hock joints for veterinary anatomical education. While plastination is a well-established method for creating durable, dry and odourless specimens, its primary limitation is the inherent rigidity of tissues, which restricts the study of dynamic joint kinematics. In contrast, the Elnady technique utilises glycerin as a primary impregnating agent to maintain lifelike flexibility. Biomechanical assessment revealed that Elnady-preserved specimens maintained stifle and hock extension/flexion angles comparable to fresh control specimens, whereas plastinated joints showed significantly restricted ranges of motion (p < 0.05). Qualitative evaluations from faculty experts (N = 20) and veterinary students (N = 60) indicated that while plastinated specimens were preferred for ease of handling and lack of odour, the Elnady specimens were rated significantly higher for tissue realism, surgical training suitability, and understanding joint positioning. The Elnady technique offers a cost-efficient and biomechanically superior alternative for teaching joint kinematics, providing a sustainable educational tool that bridges the gap between static anatomy and clinical orthopaedic practice.\u0000 </div>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 2","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147445810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Mandibular Shape Variation in Domestic Geese and Ducks Using Linear and Geometric Morphometrics 用线性和几何形态计量学对家鹅和家鸭下颌形态变异的比较分析。

IF 1 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2026-03-07 DOI: 10.1111/ahe.70100

Ece Oktay, Gülsün Pazvant, Tomasz Szara

{"title":"Comparative Analysis of Mandibular Shape Variation in Domestic Geese and Ducks Using Linear and Geometric Morphometrics","authors":"Ece Oktay, Gülsün Pazvant, Tomasz Szara","doi":"10.1111/ahe.70100","DOIUrl":"10.1111/ahe.70100","url":null,"abstract":"<div>\u0000 \u0000 In this study, a comparative assessment of species- and sex-related differentiation was conducted using two morphometric approaches based on mandibles of domestic geese (Anser anser domesticus) and domestic ducks (Anas platyrhynchos domesticus). A relatively traditional approach based on linear measurements was applied alongside a more recent method employing three-dimensional scanning and landmark-based geometric morphometric techniques. The effectiveness of both methods in discriminating morphological differences associated with species and sex was evaluated. For this purpose, a total of 80 mandibles were analysed, comprising 40 domestic geese (20 females and 20 males) and 40 domestic ducks (20 females and 20 males). In species discrimination based on linear measurements, the LS measurement was found to be statistically significant (p < 0.001). Furthermore, discriminant function analysis correctly classified domestic geese at 72.5% and domestic ducks at 87.5%. In the geometric morphometric analyses, PC1 accounted for 50% of the total shape variation, while PC2 explained 16.9%, and a clear separation between species groups was observed. In the assessment of morphological differences between sex groups, all linear measurements were significantly different between sexes in domestic geese (p < 0.001). Regarding shape variation, species groups showed a relatively homogeneous distribution within each group. Overall, this study aims to demonstrate the combined applicability of linear and geometric morphometric methods and to identify mandibular morphological differences across species and sexes, thereby contributing to research on avian morphology.\u0000 </div>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 2","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147373518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural Studies on the Pituitary Gland of Pig (Sus scrofa domestica) 猪脑垂体的超微结构研究。

IF 1 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2026-03-03 DOI: 10.1111/ahe.70098

Harideep Vaka, Santhi Lakshmi Mukku, Jagapathi Ramayya Palla, Bharathi Seelam, Reddy Dhyana Vaka, T. S. Chandrasekhara Rao, Supriya Botlagunta, Raja Kannekati, M. Kalyan Chakravarthi

{"title":"Ultrastructural Studies on the Pituitary Gland of Pig (Sus scrofa domestica)","authors":"Harideep Vaka, Santhi Lakshmi Mukku, Jagapathi Ramayya Palla, Bharathi Seelam, Reddy Dhyana Vaka, T. S. Chandrasekhara Rao, Supriya Botlagunta, Raja Kannekati, M. Kalyan Chakravarthi","doi":"10.1111/ahe.70098","DOIUrl":"10.1111/ahe.70098","url":null,"abstract":"<div>\u0000 \u0000 Among domestic animals, pigs serve as valuable biomodels for studying endocrine disorders due to similarities in the structure and hormone production of their pituitary gland with humans. The pituitary gland is a central regulator of hormonal balance and plays a crucial role in various physiological functions. The present ultrastructural studies of the porcine pituitary gland not only enhance our understanding of porcine anatomy but also provide valuable comparative data for understanding the functioning of the human pituitary gland. The pituitary gland of the pig consisted of adenohypophysis and neurohypophysis. The adenohypophysis was differentiated into pars distalis, pars intermedia, and pars tuberalis, while the neurohypophysis was divided into median eminence, infundibular stalk, and pars nervosa. Each lobe had a wide variety of hormone-secreting cells. The somatotrophs contained numerous electron-dense round secretory granules and rough endoplasmic reticula. The lactotrophs contained large electron-dense secretory granules in different shapes. The thyrotrophs contained smaller secretory granules with few mitochondria and Golgi complex. The gonadotrophs contained round electron-dense to electron-lucid secretory granules. The corticotrophs contained numerous round to oval-shaped electron-dense secretory granules and more of Golgi complex and mitochondria. In pars intermedia, granular cells, non-granular cells, elongated cells, interstitial cells, and the cleft lining cells with microvilli were noticed, whereas in the pars tuberalis, granulated cells, non-granulated cells and interstitial cells were observed. In the pars nervosa, numerous bundles of non-myelinated axons and few myelinated axons along with pituicytes were noticed. The axon terminals contained electron-dense to electron-lucid Herring bodies and numerous mitochondria.\u0000 </div>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 2","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147345711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Radiographic Morphometry of the Proximal Femur and Hip Joint in Spitz Breed Dogs: Establishing Breed-Specific Reference Values 斯皮兹犬股骨近端和髋关节的x线形态测量：建立品种特有的参考值。

IF 1 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2026-02-28 DOI: 10.1111/ahe.70096

K. S. Kamalesh Kumar, Rohit Kumar, T. Sai Kumar, Aman Kumar Tiwari, Bhanu Pratap Singh, Abhishek C. Saxena, Abhijit Pawde, Rekha Pathak, Amarpal

{"title":"Radiographic Morphometry of the Proximal Femur and Hip Joint in Spitz Breed Dogs: Establishing Breed-Specific Reference Values","authors":"K. S. Kamalesh Kumar, Rohit Kumar, T. Sai Kumar, Aman Kumar Tiwari, Bhanu Pratap Singh, Abhishek C. Saxena, Abhijit Pawde, Rekha Pathak,  Amarpal","doi":"10.1111/ahe.70096","DOIUrl":"10.1111/ahe.70096","url":null,"abstract":"<div>\u0000 \u0000 Variations in hip joint morphology are clinically important because they influence both the diagnosis of orthopaedic disease and the success of surgical interventions. Establishing breed- and gender-specific reference values is therefore essential for understanding normal canine hip anatomy and its variations. The present study aimed to provide radiographic morphometric reference data for the proximal femur and acetabulum in healthy Spitz breed dogs. Sixty adult dogs (30 males, 30 females) underwent standardised ventrodorsal extended hip radiographs under general anaesthesia. Acetabular and femoral morphological parameters were measured, and mean ± SD values were compared between genders and between left and right sides. Most parameters showed no significant differences across groups; however, femoral length, femoral neck length, and right-sided acetabular offset differed significantly between genders. Combined acetabular parameters (depth index, centre-edge angle) also showed gender-specific variation, whereas combined femoral parameters did not. These results provide the first comprehensive radiographic morphometric dataset for the Spitz breed and demonstrate subtle gender-related differences with minimal left–right asymmetry. We hypothesise that these reference values will support future comparative morphometric studies and contribute to preclinical orthopaedic research, while offering a foundation for the potential development of breed-specific surgical planning tools.\u0000 </div>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 2","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147319053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Retirement Tribute to Professor Fred Sinowatz 向弗雷德·辛诺瓦茨教授致敬。

IF 1 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2026-02-18 DOI: 10.1111/ahe.70095

William Pérez, Johannes Seeger, Pieter Cornillie, Brooke H. Dubansky, Francesco Abbate, Catrin S. Rutland

引用次数: 0

Beyond IHC Stain: Mitochondria as a Histochemical Biomarker for Apoptosis Detection in Hepatic Tissue Sections 超越免疫组化染色：线粒体作为肝组织切片细胞凋亡检测的组织化学生物标志物。

IF 1 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2026-02-16 DOI: 10.1111/ahe.70094

Maria-Cătălina Matei-Lațiu, Andreea Muntean, Vasile Rus, Adrian Florin Gal

{"title":"Beyond IHC Stain: Mitochondria as a Histochemical Biomarker for Apoptosis Detection in Hepatic Tissue Sections","authors":"Maria-Cătălina Matei-Lațiu, Andreea Muntean, Vasile Rus, Adrian Florin Gal","doi":"10.1111/ahe.70094","DOIUrl":"10.1111/ahe.70094","url":null,"abstract":"Apoptosis plays a critical role in maintaining tissue homeostasis and preventing pathological conditions. The accurate detection of apoptosis is crucial for both research and diagnostic pathology, yet conventional histological staining methods often lack the sensitivity to identify early apoptotic changes. This study aimed to evaluate and compare the effectiveness of classical and emerging mitochondria-targeting histological techniques in detecting apoptotic hepatocytes. Paraffinated hepatic tissue from five rabbits was analysed using haematoxylin–eosin (H&E), Goldner's trichrome (GT), immunohistochemistry for caspase-3 (IHC-Casp3) and Heidenhain's iron haematoxylin (HIH) staining. The percentage of apoptotic hepatocytes was quantified using two assessment methods, and statistical analyses determined the sensitivity of staining protocols. Histologically, the main apoptotic features identified in HIH-stained hepatic specimens were fading ‘ghost’ mitochondria throughout the cytoplasm, chromatin condensation, fragmentation and dissolution and nucleolar margination. A similar proportion of apoptotic hepatocytes on the HIH staining compared to IHC-Casp3 (19.00% ± 0.7 for HIH vs. 18.50% ± 0.7 for IHC-Casp3) was observed. In comparison, significantly lower values were obtained in H&E (5.75%) and GT (7.5%) stains. The lack of statistical significance of HIH vs. IHC-Casp3 demonstrates similar sensitivities. The additional quantitative analysis methods confirmed IHC as the most sensitive method (3.36 ± 3.82 apoptotic cells/field), followed by HIH, H&E, and GT. In situ histological evaluation of apoptosis remains challenging in standard H&E and GT-stained sections. HIH stain, as a cost-effective alternative to IHC, highlights early stages of apoptotic cells, providing a significant advantage over classical staining methods, emphasising their importance in diagnostic histopathology.","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"55 2","pages":""},"PeriodicalIF":1.0,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12908094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146203655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0