Anatomia Histologia Embryologia最新文献

{"title":"Age-dependent changes in the brain of albino rats (Rattus norvegicus): A morphometric analysis and magnetic resonance imaging","authors":"Amira M. Mohamad,&nbsp;Said A. Hassan,&nbsp;Eid Ali Moussa,&nbsp;Tarek Omar El Mahdy","doi":"10.1111/ahe.13014","DOIUrl":"https://doi.org/10.1111/ahe.13014","url":null,"abstract":"<p>The current manuscript had been conducted to evaluate the effect of ageing on the brains of albino rats on the macro-morphometric level and to assess the different parameters of brain and brain structures affected by ageing. Fifty apparently healthy rats of both sexes (27 males and 23 females) were used in the study, in addition to three rats for MRI. The animals were divided into four groups according to their age. Magnetic resonance imaging and cross sections of the brain were performed to document dimensions of various brain structures such as the hippocampus, surface area of lateral ventricle as well as dorsal and piriform cortices. Ageing would result into a negative impact on some brain structures. However, some others had not been affected as much. Furthermore, sex of the animal presented an important role as a variant where male rats of each age group demonstrated different results than their analogous female rats. In addition, MRI examination of the rat brain allowed recognition of the major cerebral structures. Findings of this work might be considered as a trial to provide a proof of changes resulted from normal ageing in both male and female rats and which sex is more affected than the other.</p>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"53 2","pages":""},"PeriodicalIF":0.9,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139494467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology of 16-cell embryo in bovine: Inside cells, compaction, fragmentation and effects of X-sorted semen","authors":"Clara Slade Oliveira,&nbsp;Naiara Zoccal Saraiva,&nbsp;Leticia Zoccolaro Oliveira","doi":"10.1111/ahe.13015","DOIUrl":"10.1111/ahe.13015","url":null,"abstract":"<p>In mouse embryos, inside cells are allocated in 16-cell embryos through a well-orchestrated sequence of events involving compaction and polarization. The emergence of inside cells is of great importance as itl later gives rise to the inner cell mass and epiblast. In this study, we report the sequence of critical events in embryology (compaction, inside cells allocation and fragmentation) in bovine 72 h.p.i. 9–16 cell embryos, while also investigating the effects of X-sorted semen on these events. We found a wide distribution of total cell numbers among embryos, attributed to an asynchronous cleavage pattern and blastomere death. Additionally, 13% of embryos displayed irregular shapes. The establishment of the inside cell compartment increased (<i>p</i> &lt; 0.01) in embryos with more cells. However, only 53.8% of 16-cell embryos presented inside cells. Compaction was present in 32.4% embryos and was positively correlated (<i>p</i> = 0.03, OR 3.02) with the establishment of inside cells, occurring independently of cell number. Fragmentation was present in 36% embryos, being more frequent (<i>p</i> = 0.01) in embryos with lower cell numbers. A possible association between irregular shape and fragmentation was considered (<i>p</i> = 0.06). The use of X-sorted semen had no effect on most evaluated parameters. However, it did have a marked effect on cleavage rate (<i>p</i> &lt; 0.01) and the arrest of 2- and 4- cell embryos. In conclusion, bovine embryos exhibit an asynchronous cleavage pattern, high levels of fragmentation, and demonstrate compaction and inside cell allocation later in development compared to mouse embryos. Semen X-sorting has major effects on cleavage and embryo arrest. Further studies are needed to elucidate the association between irregularly shaped embryos and fragmentation, as well as the effects of sex on inside cell allocation.</p>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"53 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139479560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computed tomographic study analysing functional biomechanics in the thoracolumbar spine of horses with and without spinal pathology","authors":"Natalie Baudisch,&nbsp;Lukas Schneidewind,&nbsp;Sophie Becke,&nbsp;Max Keller,&nbsp;Milena Overhoff,&nbsp;Dennis Tettke,&nbsp;Viola Gruben,&nbsp;Fabienne Eichler,&nbsp;Henning Jürgen Meyer,&nbsp;Christoph Lischer,&nbsp;Anna Ehrle","doi":"10.1111/ahe.13016","DOIUrl":"10.1111/ahe.13016","url":null,"abstract":"<p>To better understand physiological and pathological movement patterns in the equine thoracolumbar spine, investigation of the biomechanics on a segmental level requires a constant moment. A constant moment along the spinal column means that the same torque acts on each vertebral segment, allowing the range of motion of different segments to be compared. The aims of this study were to investigate the range of motion of the equine thoracolumbar spine in horses with and without spinal pathology and to examine whether the pressure between the spinous processes depends on the direction of the applied moment. Thoracolumbar spine specimens (T8-L4) of 23 horses were mounted in a custom-made mechanical test rig to investigate spinal biomechanics during lateral bending, axial rotation, flexion and extension using computed tomographic imaging. Results were compared between horses with spondylosis, overriding spinous processes and specimens free of gross pathology. The interspinous space pressure was additionally determined using a foil sensor. The median lateral bending between T9 and L3 was 3.7°–4.1° (IQR 5.4°–8.0°). Maximum rotational movement with inconsistent coupled motion was observed at T9–T16 (<i>p</i> &lt; 0.05). The dorsoventral range of motion was greatest in segments T9–T11 (<i>p</i> &lt; 0.05). Spondylosis and overriding spinous processes restricted spinal mobility, depending on the severity of the condition. There was no significant difference in interspinous pressure during motion (<i>p</i> = 0.54). The biomechanical study confirmed that the range of motion of intervertebral joints depends on the anatomical position of the joint and the direction of the moment applied. Restricted mobility was evident in the presence of different grades of overriding spinous processes or spondylosis. A better understanding of equine spinal biomechanics in horses with spinal pathology facilitates individual rehabilitation.</p>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"53 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ahe.13016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139479609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"List of reviewers 2023","authors":"","doi":"10.1111/ahe.13012","DOIUrl":"https://doi.org/10.1111/ahe.13012","url":null,"abstract":"","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"53 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139399964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential distribution of intermediate filament proteins in the bovine and ovine tongues","authors":"Fatma Çelenk,&nbsp;Berna Güney Saruhan,&nbsp;Hakan Sağsöz","doi":"10.1111/ahe.13013","DOIUrl":"https://doi.org/10.1111/ahe.13013","url":null,"abstract":"<p>Intermediate filaments constitute the most heterogeneous class among the major classes of cytoskeletal proteins of mammalian cells. The 40 or more intermediate filament proteins have been classified into five types which show very specific rules of expression in specialized cell types. This study aimed to investigate the immunohistochemical distribution of cytokeratins (CKs) 8, 18, and 19 as well as the intermediate filaments vimentin, laminin, and desmin in bovine and ovine tongues. Immunohistochemical staining was performed for CKs 8, 18, 19, vimentin, laminin, and desmin. Our results revealed similar immunostaining intensity and distribution among various CKs, contrasting with distinct patterns for vimentin, laminin, and desmin. Immunoreactions were primarily localized in serous acini and ductal epithelium for cytokeratins, while vimentin and laminin were evident in connective tissue, endothelium, serous acini, and desmin in striated and smooth muscles. This study highlighted the absence of CKs 8, 18, 19, vimentin, and desmin in the lingual epithelium of bovine and ovine tongues. These findings enabled the classification of epithelial cells based on their specific cytokeratin patterns. Furthermore, vimentin was identified in mesodermal tissues and organs, desmin in muscle tissue, and laminin played crucial roles in basement membrane formation, nerve tissue regeneration, innervation of epithelial taste buds, and tissue separation and connection. Our findings provide essential insights into intermediate filament dynamics at the cellular and tissue levels. They serve as a foundation for future studies using systematic molecular biological techniques in this field.</p>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"53 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139109975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiographic and sonographic features, and histogram parameters of the liver and spleen in healthy Toco toucans (Ramphastos toco, Müller 1976)","authors":"Jeana Pereira da Silva,&nbsp;Sheila Canevese Rahal,&nbsp;Anneke Moresco,&nbsp;Maria Cristina Reis Castiglioni,&nbsp;Michel de Campos Vettorato,&nbsp;Luna Scarpari Rolim,&nbsp;Ricardo Shoiti Ichikawa,&nbsp;Maria Jaqueline Mamprim","doi":"10.1111/ahe.13011","DOIUrl":"10.1111/ahe.13011","url":null,"abstract":"<p>This study aimed to evaluate radiographic and sonographic features, and histogram parameters based on grayscale ultrasound of the liver and spleen in healthy toco toucans. Fifteen adult toco toucans (<i>Ramphastos toco</i>), seven females and eight males, weighing approximately 650 g, were enrolled in the study. On the right lateral radiographic view, the liver was visualized in the midventral region of the coelomic cavity; ultrasonographically, the liver was located in the middle portion of the coelomic cavity in close relationship to the heart, and thoracic and abdominal air sacs. Two hepatic lobes were identified; the right lobe was larger than the left one. The spleen was visualized in 10 toco toucans on radiographs and only in eight toucans on ultrasound exams. The gallbladder was identified only on ultrasound. On the right lateral radiographic view, the spleen was visualized dorsal to the proventriculus and ventral to the lungs/air sacs as an oval shape. Ultrasonographically, the spleen was observed caudal to the liver, cranial to the proventriculus and craniodorsal to the ventriculus. There was no significant difference in spleen length for either radiographic or ultrasound measurements. The brightness intensity (BI) for mean gray level (G) and standard deviation of gray levels (S_G) in the liver were 101.315 (± 16.170) and 12.453 (± 2.616), respectively. Mean G and S_G levels in the spleen were 63.940 (± 18.321) and 7.494 (± 3.595), respectively. In conclusion, the sonographic features and histogram parameters indicated that the liver is more echogenic and heterogeneous than the spleen, which must be considered for diagnosing alterations in these organs.</p>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"53 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139077762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural characteristics and morphological relationships of cardiomyocytes and telocytes in the myocardium of the bullfrog (Rana catesbeiana)","authors":"Zifan Wang,&nbsp;Yizhen Xu,&nbsp;Ling Huang,&nbsp;Jiancheng Zhao,&nbsp;Yaqiong Ye,&nbsp;Canying Liu,&nbsp;Bingyun Wang,&nbsp;Haiquan Zhao,&nbsp;Hui Zhang","doi":"10.1111/ahe.13008","DOIUrl":"https://doi.org/10.1111/ahe.13008","url":null,"abstract":"<p>Telocytes (TCs) are distinctive interstitial cells due to their characteristic structures and heterogeneity. They are suggested to participate in tissue repair/regeneration. TCs have been identified in many organs of various mammals. However, data on TCs in lower animals are still very limited. In this work, TCs were identified in the myocardium of the bullfrog (<i>Rana catesbeiana</i>) by light and transmission electron microscopy (TEM). The structural relationships between TCs and neighbouring cell types were measured using the ImageJ (FiJi) morphometric software. TCs with slender Tps (telepodes) were located around cardiomyocytes (CMC). TEM revealed TCs with long Tps in the stroma between CMC. The homocellular tight junctions were observed between the Tps. The Tps were also very close to the neighbouring CMC. The distance between Tps and CMC was 0.15 ± 0.08 μm. Notably, Tps were observed to adhere to the periphery of the satellite cells. The Tps and the satellite cells established heterocellular structural connections by tight junctions. Additionally, Tps were frequently observed in close proximity to mast cells (MCs). The distance between the Tps and the MCs was 0.19 ± 0.09 μm. These results confirmed that TCs are present in the myocardium of the bullfrog, and that TCs established structural relationships with neighbouring cell types, including satellite cells and MCs. These findings provide the anatomical evidence to support the note that TCs are involved in tissue regeneration.</p>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"53 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138679042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sonographic features of abdominal organs in healthy captive Neotropical otters (Lontra longicaudis)","authors":"Beatriz de Espírito Augusti,&nbsp;Sheila Canevese Rahal,&nbsp;Jeana Pereira da Silva,&nbsp;Mirian Halásc Vac,&nbsp;Rodrigo Hidalgo Friciello Teixeira,&nbsp;Andre Luiz Mota da Costa,&nbsp;Marcelo da Silva Gomes,&nbsp;Jeniffer Novais,&nbsp;Oldemar Carvalho Junior,&nbsp;Maria Jaqueline Mamprim","doi":"10.1111/ahe.13009","DOIUrl":"https://doi.org/10.1111/ahe.13009","url":null,"abstract":"<p>This study aimed to assess the sonographic features of abdominal organs in healthy captive Neotropical otters (<i>Lontra longicaudis</i>). The sonograph showed that the urinary bladder was located in the caudal abdomen and its content was uniformly anechoic. The bladder wall had three layers: outer hyperechoic serosa, hypoechoic muscular layer and hyperechoic mucosa. The mean total wall thickness was 0.10 cm. The splenic parenchyma had a homogeneous echotexture with greater echogenicity than that of the left renal cortex and liver. The borders were regular and tapered. Rugal folds were observed in the stomach when undistended. The mean gastric wall thickness was 0.28 cm and five distinct layers were visible. The liver had smooth contours, and division of the hepatic lobes was not precisely visualized. The hepatic parenchyma had a homogeneous echotexture with greater echogenicity as compared to the right kidney and lesser echogenicity than that of the spleen. The gallbladder appeared as an oval or rounded structure in the transverse plane, filled with homogeneous anechogenic content; the wall was thin, regular and hyperechoic, with a mean thickness of 0.09 cm. The kidneys had a lobulated appearance with renicular subunits. The mean total length of the kidneys in longitudinal plane was 6.18 cm for the left and 6.27 cm for the right. Each reniculus was covered by an echogenic capsule, and the medullary region was more hypoechogenic than the cortical region. In conclusion, sonographic features in most of the abdominal organs in Neotropical otters are similar to the ultrasound patterns observed in healthy dogs and cats, except for kidneys with reniculi.</p>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"53 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138679048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological features of the late-embryo-stage gastrointestinal tract of free-roaming light ecotype Nigerian indigenous chicken","authors":"Anietie Francis Udoumoh,&nbsp;Udensi Maduabuchi Igwebuike,&nbsp;Innocent Chima Nwaogu,&nbsp;Ikechukwu Reginald Obidike","doi":"10.1111/ahe.13007","DOIUrl":"10.1111/ahe.13007","url":null,"abstract":"<p>Morphological changes in the late-embryo-stage gastrointestinal tract of Nigerian indigenous chicken were investigated using anatomical techniques. The paraffin-embedded sections were stained with haematoxylin and eosin and periodic acid Schiff-Alcian blue (pH 2.5) stains. During the late pre-hatch period, the framework of the stomach was already established, but glands were yet poorly developed. Randomly oriented pre-glandular cells in the lamina propria mucosae at embryonic day (ED) 14 became organized into elongated simple tubular glands at ED 19 and assumed adult morphology at post-hatch day (PD) 1. Acidic and neutral mucin deposits were associated with the glandular cells. In the small intestine, enterocytes of the tunica epithelialis mucosae transformed from cuboidal-shaped cells (at ED 14) to tall columnar cells (at PD 1). Short crypts of Lieberkühn, goblet cells, microfold cells and enteroendocrine cells were evident at ED 19, while the lamina propria mucosae and submucosa contained mesenchymal cells, reticular cells and isolated lymphoblasts. Similarly, the crypts, lymphoblasts, mesenchymal and reticular cells were also associated with the lamina propria mucosae and submucosa of the caecum and colorectum at ED 19. It was inferred from these findings that extensive cellular and tissue modifications occur in the gastrointestinal tract within the narrow window of the late pre-hatch period. The definitive gut tunics, epithelium, glandular tissues, immune-competent cells and tissues are formed as a result. Thus, the embryonic gut of the bird is made capable of assuming its necessary functions of food digestion, nutrient absorption, water and ion re-absorption, immune surveillance, antibody production and immune responses at hatch.</p>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"53 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138563485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microanatomical, histochemical and morphometric features of the major and selected minor salivary glands in laboratory Wistar rat","authors":"Gal Adrian Florin,&nbsp;Matei-Latiu Maria-Catalina,&nbsp;Miclaus Viorel,&nbsp;Rus Vasile,&nbsp;Latiu Calin,&nbsp;Ratiu Ioana Adela","doi":"10.1111/ahe.13006","DOIUrl":"10.1111/ahe.13006","url":null,"abstract":"<p>The major and minor salivary glands are responsible for saliva production, a fluid drained in the oral cavity that will be involved in several functions at this level. The present study aimed to compare the microanatomy of the major (parotid, mandibular, sublingual) and minor salivary glands (von Ebner's and Weber's) of the Wistar rat, from a histological, histochemical and morphometrically point of view. Predominantly serous glands (parotid, mandibular and von Ebner's) showed differences in the secretion composition on both PAS and alcian blue reactions. The same observations were identified also for the mostly mucous salivary glands (sublingual and Weber). In terms of surface dimensions, the measurements suggested that the mucous acini have a greater surface compared with the serous ones, and, at the same time, a better-represented polymorphism. In conclusion, the major and minor salivary glands in Wistar rats, a commonly used species in experimental procedures, showed similarities with other species including humans, but some structural traits are breed-specific, this finding is very important for an extensive understanding of the morphophysiology of the salivary glands.</p>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"53 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138563856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}