Anatomia Histologia Embryologia最新文献

Three-Dimensional Morphometrical Study on Small Intestine in Rabbit Using Unbiased Design-Based Stereology

IF 0.8 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2025-03-04 DOI: 10.1111/ahe.70025

Javad Sadeghinezhad, Farhad Monsef, Hadis Bojarzadeh

{"title":"Three-Dimensional Morphometrical Study on Small Intestine in Rabbit Using Unbiased Design-Based Stereology","authors":"Javad Sadeghinezhad, Farhad Monsef, Hadis Bojarzadeh","doi":"10.1111/ahe.70025","DOIUrl":"https://doi.org/10.1111/ahe.70025","url":null,"abstract":"<div>\u0000 \u0000 Three-dimensional structural parameters correlate better with the intestinal function compared to two-dimensional structural parameters. The objective of the present study was to investigate the three-dimensional morphometrical features of the small intestine in rabbits using a design-based stereology. Five adult male New Zealand rabbits were used in this research. The total volume, volume fraction of different layers, internal surface area, villus and circular folds amplification factors were estimated in the whole and various segments of the small intestine. The small intestine volume was estimated to be 59.57 ± 8.57 cm3. The jejunum and ileum showed the most and the least total volumes, respectively (p < 0.05). The volume fractions of the tunica mucosa, tela submucosa and tunica muscularis were 62.57% ± 2.78%, 21.11% ± 2.01% and 16.25% ± 0.94%, respectively. The minimum volume of the tela submucosa was observed in the ileum (p < 0.05) and the fractional volume of the tunica muscularis in the ileum was significantly more than those of other segments (p < 0.05). The surface area of the entire small intestine was estimated to be 8302.31 ± 1872.39 cm2. The jejunum and ileum segments showed the most and the least surface areas, respectively. The increase in the intestinal surface area attributable to the presence of villi was estimated to be 6.82 ± 0.84, and that for circular folds was accounted for 1.36 ± 0.09. The largest amplification factors of villi and circular folds were observed in the duodenum and ileum, respectively. Our data presented the three-dimensional morphometry of the small intestine of the rabbit as a translational model and provided basic findings for the diagnosis and treatment of intestinal diseases in this species.\u0000 </div>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"54 2","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Developmental Changes in the Morphology of the Asterisci Otoliths of Cyprinion kais (Heckel, 1843) and Cyprinion macrostomum (Heckel, 1843) Collected From Tigris River, Şırnak Province, Türkiye

IF 0.8 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2025-03-03 DOI: 10.1111/ahe.70029

Laith A. Jawad, Muhammed Y. Dörtbudak

引用次数: 0

Immunohistochemical Distribution of Heat Shock Proteins 27, 60, 70 and 90 in the Placenta of Goats in the Second and Third Trimester of Pregnancy

IF 0.8 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2025-02-27 DOI: 10.1111/ahe.70027

Banu Kandil

{"title":"Immunohistochemical Distribution of Heat Shock Proteins 27, 60, 70 and 90 in the Placenta of Goats in the Second and Third Trimester of Pregnancy","authors":"Banu Kandil","doi":"10.1111/ahe.70027","DOIUrl":"https://doi.org/10.1111/ahe.70027","url":null,"abstract":"<div>\u0000 \u0000 Determining the immunoexpression of HSPs in the placenta may contribute to the understanding of pregnancy physiology and immunotolerance mechanisms. This study aimed to determine the distribution of HSP27, HSP60, HSP70 and HSP90 in the placenta of goats. Tissue samples were taken from the placentomal and interplacentomal regions of the pregnant uterus of 12 goats, comprising 6 s and 6 third trimesters. Following routine histological procedures, immunohistochemical staining was performed on tissue sections. In the interplacentomal region, HSP27, HSP60, HSP70 and HSP90 in luminal and glandular epithelial, stromal and smooth muscle cells did not show differences between the second and third trimesters (p > 0.05). In the placentomal region, HSP27 and HSP60 in syncytial plaques and HSP27, HSP60, HSP70, and HSP90 in maternal stromal cells did not change as the pregnancy progressed (p > 0.05). There was no difference in HSP27, HSP60 and HSP90 in fetal stromal cells between the second and third trimesters (p > 0.05). HSP27 and HSP90 were positive in both trophoblast cells, HSP60 was positive in binucleate trophoblast cells, and HSP70 was positive in mononucleate trophoblast cells. In the third trimester compared with the second trimester, HSP27 was decreased (p < 0.05), while HSP90 in mononucleate and binucleate trophoblast cells did not show a difference (p > 0.05). HSP70 did not change in mononucleate trophoblast cells (p > 0.05), but HSP60 was increased in binucleate trophoblast cells (p < 0.05) as the pregnancy progressed. In conclusion, this study showed that HSPs had similar immunoexpression patterns in the interplacentomal region but different immunoexpression patterns in the placentomal region of the goat placenta.\u0000 </div>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"54 2","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological, Histological and Histochemical Analysis of the Digestive Tract in Panga Pangasius hypophthalmus (Teleostei: Siluriformes) 鲮鱼消化道的形态学、组织学和组织化学分析（远洋鱼类：丝形目）

IF 0.8 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2025-02-26 DOI: 10.1111/ahe.70026

Lucas Camilo Moraes Alves, Phâmella Neres de Lima, Matheus Santos Costa, Anderson Pires de Moraes, Fernanda Gomes de Paula, Edson José Benetti, Karina Simões

{"title":"Morphological, Histological and Histochemical Analysis of the Digestive Tract in Panga Pangasius hypophthalmus (Teleostei: Siluriformes)","authors":"Lucas Camilo Moraes Alves, Phâmella Neres de Lima, Matheus Santos Costa, Anderson Pires de Moraes, Fernanda Gomes de Paula, Edson José Benetti, Karina Simões","doi":"10.1111/ahe.70026","DOIUrl":"https://doi.org/10.1111/ahe.70026","url":null,"abstract":"<div>\u0000 \u0000 The current study aims to describe the macroscopic, histological and histochemical features of the digestive tract of omnivorous fish species Panga Pangasius hypophthalmus—which derives from Southeast Asian watersheds—with emphasis on secretory cell types and their histochemical reactions. Results have evidenced that the anatomical organisation of the digestive tract in the coelomic cavity of the investigated species is similar to that of other omnivorous teleosts, except for a pneumatic duct linking the distal portion of their oesophagus to their swim bladder. It is worth highlighting that both the oesophagus and the stomach have well-developed histological layers and that the stomach has a saccular shape and is highly distensible to ensure longer food storage time. Intestinal loops are convoluted and present well-developed mucosal folds with varying widths, heights and thicknesses; these features increase the nutrient absorption surface. The digestive tract presented histological and histochemical features similar to those of other siluriform fish that show the same feeding habits and secretory cells throughout the digestive tract, whose acid and neutral secretions guarantee the functionality of digestive processes.\u0000 </div>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"54 2","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Local Anaesthesia of the Bovine Tarsus: A Cadaver Study Comparing Anatomical Landmark-Based and Ultrasound-Guided Nerve Blocks 牛跗骨局部麻醉：基于解剖地标和超声引导神经阻滞的尸体研究比较

IF 0.8 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2025-02-26 DOI: 10.1111/ahe.70028

Karl Klisch, David Winkelmeyer, Karl Nuss

{"title":"Local Anaesthesia of the Bovine Tarsus: A Cadaver Study Comparing Anatomical Landmark-Based and Ultrasound-Guided Nerve Blocks","authors":"Karl Klisch, David Winkelmeyer, Karl Nuss","doi":"10.1111/ahe.70028","DOIUrl":"https://doi.org/10.1111/ahe.70028","url":null,"abstract":"<div>\u0000 \u0000 Surgical procedures involving the tarsal joint are common in cattle. Indications for tarsal surgery include joint lavage, arthrotomy, the treatment of injuries, and hygromas. The goal of the present study was to lay the foundation for a reliable method of nerve block desensitisation of the bovine tarsus. Two nerve block techniques were simulated in an anatomical cadaver study on six bovine pelvic limbs for each technique. In the first technique, injection sites were based on palpation of anatomical structures, and in the second technique, ultrasound guidance was used to localise the nerves. After injection of dye (methylene blue) solution using anatomical landmark-based or ultrasound guidance methods, the 12 cadaver limbs were dissected, and the effectiveness of sham anaesthesia was determined based on the proximity of the dye solution to the nerve and the length of its distribution along the nerve. The ultrasound-guided local anaesthesia required significantly more time to perform compared with nerve blocks based on anatomical landmarks. However, the dye reached the target area in 25 of 30 application sites using ultrasound-guided local anaesthesia compared with 13 of 30 sites using the anatomical landmark-based technique. Ultrasonographic guidance led to fewer inadvertent intravascular and intramuscular injections. In conclusion, the ultrasound-guided local anaesthesia is a promising alternative to general anaesthesia, epidural, and intravenous regional anaesthesia for surgical procedures of the bovine tarsal joint and eliminates the disadvantages of other anaesthetic techniques, like general anaesthesia and intravenous regional anaesthesia. The practicability and utility of this technique still require validation in vivo.\u0000 </div>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"54 2","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Telocytes in the Ovary, Oviduct and Uterus of Kyrgyz Mares According to Pregnancy Status

IF 0.8 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2025-02-19 DOI: 10.1111/ahe.70024

Ali Risvanli, Fatih Hatipoglu, Ruslan Salykov, Necati Timurkaan, Nariste Kadiralieva, Kaliyman K. Kasymalieva, Ibrahim Seker, Elif Ekinci

{"title":"Evaluation of Telocytes in the Ovary, Oviduct and Uterus of Kyrgyz Mares According to Pregnancy Status","authors":"Ali Risvanli, Fatih Hatipoglu, Ruslan Salykov, Necati Timurkaan, Nariste Kadiralieva, Kaliyman K. Kasymalieva, Ibrahim Seker, Elif Ekinci","doi":"10.1111/ahe.70024","DOIUrl":"https://doi.org/10.1111/ahe.70024","url":null,"abstract":"<div>\u0000 \u0000 In the current study, we aimed to determine the distribution of telocytes in the ovary, oviduct and uterus of Kyrgyz mares depending on whether they were pregnant. We also studied the progression during the first 6 months of pregnancy. To this end, ovary, oviduct and uterus tissues from 53 Kyrgyz mares were obtained post-mortem. The mare tissue samples were divided into two groups: pregnant (n = 43) and non-pregnant (n = 10). Telocyte levels in the tissues were determined using light microscopic and immunohistochemical methods. Subsequently, the data acquired were evaluated based on whether the mare was pregnant, and if so, the month of pregnancy was also taken into account. After the study, we found a higher number of telocytes in the myometrium (5.69 ± 0.90, p < 0.001) and oviduct propria (0.14 ± 0.05, p < 0.006) of pregnant mares. We also noted an increased number of telocytes in the oviduct propria (0.04 ± 0.02, p < 0.03) of mares 4 months into the pregnancy, and in the muscle layer (1.62 ± 0.57, p < 0.01), myometrium (4.83 ± 0.78, p < 0.03) and ovarian stroma (0.14 ± 0.06, p < 0.04) of mares 6 months into pregnancy. In conclusion, we observed an increase in telocytes in the ovaries, oviducts and uterus of Kyrgyz mares during pregnancy, with this increase becoming more marked towards the sixth month of gestation. Therefore, additional research on the role of telocytes in pregnancy may prove beneficial.\u0000 </div>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"54 2","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Radiologic Anatomy of the Thorax of the Southern Giant Pouched Rat (Cricetomys ansorgei)

IF 0.8 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2025-02-12 DOI: 10.1111/ahe.70023

Vumilia J. Kibona, Modesta Makungu

{"title":"Radiologic Anatomy of the Thorax of the Southern Giant Pouched Rat (Cricetomys ansorgei)","authors":"Vumilia J. Kibona, Modesta Makungu","doi":"10.1111/ahe.70023","DOIUrl":"https://doi.org/10.1111/ahe.70023","url":null,"abstract":"<div>\u0000 \u0000 The southern giant pouched rat (Cricetomys ansorgei) is a large rodent native to the savannahs of southern and East Africa, commonly kept as a pet and used for medical purposes, including the detection of pulmonary tuberculosis, Brucella infection, and land mines. Despite its biomedical significance, limited information exists on its thoracic radiological anatomy. This study aimed to describe the normal thoracic radiographic anatomy of Cricetomys ansorgei to support anatomical and clinical research. Radiographs of 34 adult southern giant pouched rats were taken under general anaesthesia, including right lateral (RL) and dorsoventral (DV) views of the thorax. Most rats exhibited 13 thoracic vertebrae and 13 pairs of ribs, with the last two floating pairs. All rats displayed six sternebrae, and the trachea was visible without mineralised cartilaginous rings. The cranial mediastinum appeared wider than the spine in all individuals. The cranial border of the cardiac silhouette was poorly visualised on the RL view but more clearly defined and oblong-shaped on the DV view. The mean vertebral heart size (VHS) measured on the DV view (10.59) was significantly larger than that measured on the RL view (9.49). Additionally, gravel and sand grains were commonly observed in the cranial abdomen. While the thoracic anatomy of Cricetomys ansorgei shares some characteristics with other rodents, rabbits, and domestic cats, several distinctive anatomical features were identified. These findings provide a valuable reference for anatomical studies, biomedical applications, and routine clinical assessments.\u0000 </div>","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"54 2","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transosomes Contribute to the Inner Perivitelline Layer of the Quail (Coturnix japonica) Oocyte: An Ultrastructural and Immunohistochemical Study

IF 0.8 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2025-02-11 DOI: 10.1111/ahe.70020

Daniela Rodler, Anna Teresa Driese-Haydn

引用次数: 0

Phenotyping the Sheep Tail: Histological Depiction of Caudal Spine Structures in Sheep

IF 0.8 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2025-02-06 DOI: 10.1111/ahe.70022

Hannah Hümmelchen, Henrik Wagner, Sabine Wenisch, Sven König, Axel Wehrend

{"title":"Phenotyping the Sheep Tail: Histological Depiction of Caudal Spine Structures in Sheep","authors":"Hannah Hümmelchen, Henrik Wagner, Sabine Wenisch, Sven König, Axel Wehrend","doi":"10.1111/ahe.70022","DOIUrl":"https://doi.org/10.1111/ahe.70022","url":null,"abstract":"The morphology of sheep tails remains relatively understudied. Given the escalating discourse on routine removal of the caudal spine in sheep, a thorough exploration of the anatomical structures within this region is imperative. To examine the tails in detail, this study undertook histological characterisation of three segments (the cranial, middle, and caudal segments) of the tails of 12 undocked Merino sheep lambs. Six lambs were selected for having short tails (37.1 ± 3.0 cm) while the remaining six were chosen for their long tails (49.4 ± 1.7 cm). Immunohistochemical labelling using neuron-specific enolase antibodies was performed to examine the nerve structures in the tail tip. The general structure of the skin resembles that of other domestic mammals. Interestingly, many sweat and sebaceous glands were found in all three tail segments. These findings support the hypothesis that the tails of sheep play a significant role in thermogenesis and perhaps olfactory communication. The study also revealed nerve fibres extending to the tip of the tail. This observation supports the requirement for pain elimination during tail tip amputation.","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"54 2","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ahe.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Expression/Regulation of Progesterone Receptor in Reproductive Tissues (Ovary and Uterus) Induced by Human Chorionic Gonadotropin and Equine Chorionic Gonadotropin Treatments in Sows

IF 0.8 4区农林科学

Anatomia Histologia Embryologia Pub Date : 2025-02-01 DOI: 10.1111/ahe.70017

Oswaldo Cruz León, Santiago René Anzaldúa Arce, Miguel Angel Cornejo Cortes, Marco Cerbón, Francisco Ernesto Martínez Castañeda, Raúl Ulloa Arvizu, María Elena Trujillo Ortega

{"title":"Differential Expression/Regulation of Progesterone Receptor in Reproductive Tissues (Ovary and Uterus) Induced by Human Chorionic Gonadotropin and Equine Chorionic Gonadotropin Treatments in Sows","authors":"Oswaldo Cruz León, Santiago René Anzaldúa Arce, Miguel Angel Cornejo Cortes, Marco Cerbón, Francisco Ernesto Martínez Castañeda, Raúl Ulloa Arvizu, María Elena Trujillo Ortega","doi":"10.1111/ahe.70017","DOIUrl":"10.1111/ahe.70017","url":null,"abstract":"We studied sows (Landrace × Yorkshire line, DanBred Hybrid) to evaluate the possible changes in progesterone receptor (PR) expression in the uterus and ovary caused by different non-hypophyseal gonadotropins treatments: equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG). Varying concentrations of eCG and hCG were evaluated (Groups 1, 2, 3, 4). PR expression was determined by immunohistochemistry, and labelling intensity was determined by the HScore method. In the ovary, PR expression in the granulosa cells of follicles did not differ significantly between Groups 1 and 2 (p < 0.05) but differed significantly from that in Groups 3 and 4 (p < 0.05), which in turn did not differ from each other. This PR expression pattern was similar across groups in the internal and external theca cells. Conversely, in the uterus, PR expression in the lining epithelium was lower in Group 4 than that in Group 1 (p < 0.05). Increased expression was observed in the endometrial lamina propria in all groups 2 and 4 compared to that in the control group (p < 0.05). Decreased expression was observed in the glandular epithelium and myometrium in Group 4 compared to that in Group 1 (p < 0.05). In the ovary, PR expression in the granulosa and outer and inner theca of the follicles was not significantly different (p < 0.05) between Groups 1 and 2 or Groups 3 and 4; however, the expression in these pairs of groups differed from each other. Thus, changes in PR expression may depend on the concentrations and proportions of exogenous hormones used in the treatments, indicating an alteration in the reproductive process.","PeriodicalId":49290,"journal":{"name":"Anatomia Histologia Embryologia","volume":"54 2","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ahe.70017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0