Biointerphases最新文献_第3页

Polystyrene-block-polyethylene oxide thin films: In vitro cytocompatibility and protein adsorption testing. 聚苯乙烯-聚块氧化聚乙烯薄膜:体外细胞相容性和蛋白质吸附试验。

IF 2.1 4区医学

Biointerphases Pub Date : 2020-01-31 DOI: 10.1116/1.5135062

Isabela Monteiro A, Tarek Kollmetz, David S Musson, Sue R McGlashan, Jenny Malmström

{"title":"Polystyrene-block-polyethylene oxide thin films: In vitro cytocompatibility and protein adsorption testing.","authors":"Isabela Monteiro A, Tarek Kollmetz, David S Musson, Sue R McGlashan, Jenny Malmström","doi":"10.1116/1.5135062","DOIUrl":"https://doi.org/10.1116/1.5135062","url":null,"abstract":"Polystyrene-block-polyethylene oxide (PS-b-PEO) coated surfaces have been explored as cell culture substrates in the past decade. However, their cytocompatibility has not been extensively assessed. In this study, the in vitro cytocompatibility of PS-b-PEO was investigated. Cellular morphology, metabolic activity, and viability were evaluated at 1, 3, and 5 days after cell seeding. Viability was greater than 90% throughout the 5 days culture, with abundant cell spreading evident by the formation of prominent F-actin stress fibres. The cytocompatibility study was complemented by the analysis of adsorption of a range of extracellular matrix proteins on PS-b-PEO thin films by quartz crystal microbalance with dissipation. Protein adsorption tests revealed that there was no significant difference in protein adhesion between surfaces with a PEO domain coverage of ≈28%, compared to the homogeneous polystyrene control. The findings demonstrate that PS-b-PEO thin films are cytocompatible and are a favourable surface coating for cell culture studies.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2020-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1116/1.5135062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37599583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Formation and characteristics of mixed lipid/polymer membranes on a crystalline surface-layer protein lattice. 晶体表面蛋白质晶格上混合脂质/聚合物膜的形成和特性。

IF 2.1 4区医学

Biointerphases Pub Date : 2020-01-16 DOI: 10.1116/1.5132390

Christian Czernohlavek, Bernhard Schuster

{"title":"Formation and characteristics of mixed lipid/polymer membranes on a crystalline surface-layer protein lattice.","authors":"Christian Czernohlavek, Bernhard Schuster","doi":"10.1116/1.5132390","DOIUrl":"10.1116/1.5132390","url":null,"abstract":"The implementation of self-assembled biomolecules on solid materials, in particular, sensor and electrode surfaces, gains increasing importance for the design of stable functional platforms, bioinspired materials, and biosensors. The present study reports on the formation of a planar hybrid lipid/polymer membrane on a crystalline surface layer protein (SLP) lattice. The latter acts as a connecting layer linking the biomolecules to the inorganic base plate. In this approach, chemically bound lipids provided hydrophobic anchoring moieties for the hybrid lipid/polymer membrane on the recrystallized SLP lattice. The rapid solvent exchange technique was the method of choice to generate the planar hybrid lipid/polymer membrane on the SLP lattice. The formation process and completeness of the latter were investigated by quartz crystal microbalance with dissipation monitoring and by an enzymatic assay using the protease subtilisin A, respectively. The present data provide evidence for the formation of a hybrid lipid/polymer membrane on an S-layer lattice with a diblock copolymer content of 30%. The hybrid lipid/polymer showed a higher stiffness compared to the pure lipid bilayer. Most interestingly, both the pure and hybrid membrane prevented the proteolytic degradation of the underlying S-layer protein by the action of subtilisin A. Hence, these results provide evidence for the formation of defect-free membranes anchored to the S-layer lattice.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2020-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1116/1.5132390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37551935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Carboxybetaine functionalized nanosilicas as protein resistant surface coatings. 羧甜菜碱功能化纳米二氧化硅作为抗蛋白质表面涂层。

IF 2.1 4区医学

Biointerphases Pub Date : 2020-01-06 DOI: 10.1063/1.5126467

Brianna R Knowles, Pawel Wagner, Shane Maclaughlin, Michael J Higgins, Paul J Molino

{"title":"Carboxybetaine functionalized nanosilicas as protein resistant surface coatings.","authors":"Brianna R Knowles, Pawel Wagner, Shane Maclaughlin, Michael J Higgins, Paul J Molino","doi":"10.1063/1.5126467","DOIUrl":"https://doi.org/10.1063/1.5126467","url":null,"abstract":"Materials with protein resistant properties are increasingly sought after for their potential application as low-fouling surface coatings. Hydrophilic coatings with improved resistance to protein fouling have been prepared from zwitterionic carboxybetaine (CB) functionalized silica nanoparticles (SiNPs). The authors report three methods of coating preparation via direct tethering of CB to predeposited particle films, a two-step surface functionalization process, and deposition of CB functionalized particle dispersions. The pH at which aqueous CB solutions were prepared and reacted to SiNPs was found to drastically influence the mechanism of CB attachment and affect the protein resistance of the resultant coatings. Depending on the method of coating preparation, protein binding to functionalized particle coatings was reduced by up to 94% compared to unfunctionalized SiNP control surfaces. As a result, all three methods offer simple and scalable fabrication routes for the generation of hydrophilic, zwitterionic interfaces with improved inhibition to protein fouling.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2020-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1063/1.5126467","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37515702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Hyaluronic acid-based hydrogels with independently tunable mechanical and bioactive signaling features. 基于透明质酸的水凝胶具有独立可调的机械和生物活性信号功能。

IF 2.1 4区医学

Biointerphases Pub Date : 2020-01-02 DOI: 10.1063/1.5126493

Madison D Godesky, David I Shreiber

{"title":"Hyaluronic acid-based hydrogels with independently tunable mechanical and bioactive signaling features.","authors":"Madison D Godesky, David I Shreiber","doi":"10.1063/1.5126493","DOIUrl":"10.1063/1.5126493","url":null,"abstract":"Extracellular matrix provides critical signaling context to resident cells through mechanical and bioactive properties. To realize the potential of tissue engineering and regenerative medicine, biomaterials should allow for the independent control of these features. This study investigates a hydrogel system based on thiol-modified hyaluronic acid (HA-S) and polyethylene glycol diacrylate (PEGDA). The mechanical properties of HAS-PEGDA are dictated by two cytocompatible crosslinking reactions that occur at distinct time points: a rapid, Michael-type nucleophilic addition reaction between HA-thiols and PEG-acrylates and a prolonged maturation of disulfide crosslinks from remaining thiols. It is hypothesized that these reactions would enable the independent tuning of the mechanical and bioactive features of HAS-PEGDA. Rheological studies confirmed that initial gelation reached completion by 1 day, at which point the shear modulus was proportional to the concentration of PEGDA. Over time, the shear modulus evolved dramatically, and final stiffness depended on the availability of HA-thiols. The addition of PEG-monoacrylate (PEGMA) after the initial gelation occupied a percentage of remaining thiols to prevent disulfide crosslinking, decreasing the steady-state stiffness in a dose-dependent manner. A fraction of the PEGMA was then replaced with acrylated peptide ligands to introduce specific bioactivity to the otherwise non-cell-adhesive network. The degree of latent stiffening was controlled by the total amount of peptide-PEGMA, while adhesivity was tuned with the balance of bioactive and inactive peptides. The functional effects of the tunable mechanical and bioadhesive ligand properties were confirmed with assays of cell adhesion and morphology.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2020-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7008889/pdf/BJIOBN-000014-061005_1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37506718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early-stage corrosion, ion release, and the antibacterial effect of copper and cuprous oxide in physiological buffers: Phosphate-buffered saline vs Na-4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid. 生理缓冲液中铜和氧化亚铜的早期腐蚀、离子释放和抗菌作用:磷酸盐缓冲盐水与Na-4-(2-羟乙基)-1-哌嗪乙烷磺酸

IF 2.1 4区医学

Biointerphases Pub Date : 2019-12-12 DOI: 10.1063/1.5123039

Jiaqi Luo, Christina Hein, Jean-François Pierson, Frank Mücklich

{"title":"Early-stage corrosion, ion release, and the antibacterial effect of copper and cuprous oxide in physiological buffers: Phosphate-buffered saline vs Na-4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.","authors":"Jiaqi Luo, Christina Hein, Jean-François Pierson, Frank Mücklich","doi":"10.1063/1.5123039","DOIUrl":"https://doi.org/10.1063/1.5123039","url":null,"abstract":"Copper surfaces are well known for their antibacterial effects due to the release of copper ions. This benefit has been shown in many antibacterial efficiency tests, however, without considering the corrosion behaviors of copper in the physiological solutions, which could play an indispensable role in ion release from the metallic surface. This study compared the ground copper surface and sputtered cuprous oxide (Cu2O) coating in two common physiological buffers: phosphate-buffered saline (PBS) and Na-4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Na-HEPES). The growth of the cuprous oxide (Cu2O) layer was found on copper in pure PBS, inhibiting further copper ion release. In contrast, a continuous release of copper ions was recorded in Na-HEPES for 3 h, where no oxide formation was observed. The antibacterial efficiency of copper (against E. coli) was measured and discussed with the ion release kinetics in the presence of E. coli. Similar results were obtained from Cu2O coating, ruling out its assisting role in showing the antibacterial property from copper surfaces, but they did indicate the importance of taking environmental parameters into consideration in interpreting the antibacterial efficiency of copper surfaces.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2019-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1063/1.5123039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37451766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Two stages in three-dimensional in vitro growth of tissue generated by osteoblastlike cells. 成骨样细胞体外三维组织生长的两个阶段。

IF 2.1 4区医学

Biointerphases Pub Date : 2010-06-01 DOI: 10.1116/1.3431524

Krishna P Kommareddy, Claudia Lange, Monika Rumpler, John W C Dunlop, Inderchand Manjubala, Jing Cui, Karl Kratz, Andreas Lendlein, Peter Fratzl

{"title":"Two stages in three-dimensional in vitro growth of tissue generated by osteoblastlike cells.","authors":"Krishna P Kommareddy, Claudia Lange, Monika Rumpler, John W C Dunlop, Inderchand Manjubala, Jing Cui, Karl Kratz, Andreas Lendlein, Peter Fratzl","doi":"10.1116/1.3431524","DOIUrl":"https://doi.org/10.1116/1.3431524","url":null,"abstract":"Bone regeneration is controlled by a variety of biochemical, biomechanical, cellular, and hormonal mechanisms. In particular, physical properties of the substrate such as stiffness and architecture highly influence the proliferation and differentiation of cells. The aim of this work is to understand the influence of scaffold stiffness and cell seeding densities on the formation of tissue by osteoblast cells within polyether urethane scaffolds containing pores of different sizes. MC3T3-E1 preosteoblast cells were seeded on the scaffold, and the amount of tissue formed within the pores was analyzed for culture times up to 49 days by phase contrast microscopy. The authors show that the kinetics of three-dimensional tissue growth in these scaffolds follows two stages and can be described by a universal growth law. The first stage is dominated by cell-material interactions with cell adherence and differentiation being strongly dependent on the polymer material. After a delay time of a few weeks, cells begin to grow within their own matrix, the delay being strongly dependent on substrate stiffness and seeding protocols. In this later stage of growth, three-dimensional tissue amplification is controlled rather by the pore geometry than the scaffold material properties. This emphasizes how geometric constraints may guide tissue formation in vitro and shows that optimizing scaffold architectures may improve tissue formation independent of the scaffold material used.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1116/1.3431524","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40061219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 58

UV laser-ablated surface textures as potential regulator of cellular response. 紫外激光消融表面织构作为细胞反应的电位调节剂。

IF 2.1 4区医学

Biointerphases Pub Date : 2010-06-01 DOI: 10.1116/1.3438080

Prafulla Chandra, Karen Lai, Hak-Joon Sung, N Sanjeeva Murthy, Joachim Kohn

引用次数: 21

Continuum model of mechanical interactions between biological cells and artificial nanostructures. 生物细胞与人造纳米结构之间力学相互作用的连续体模型。

IF 2.1 4区医学

Biointerphases Pub Date : 2010-06-01 DOI: 10.1116/1.3431960

Piyush Verma, Ian Y Wong, Nicholas A Melosh

引用次数: 19

Characterization and cytocompatibility of hybrid aminosilane-agarose hydrogel scaffolds. 杂化氨基硅烷-琼脂糖水凝胶支架的表征及细胞相容性。

IF 2.1 4区医学

Biointerphases Pub Date : 2010-06-01 DOI: 10.1116/1.3388182

V Sánchez-Vaquero, C Satriano, N Tejera-Sánchez, L González Méndez, J P García Ruiz, M Manso Silván

{"title":"Characterization and cytocompatibility of hybrid aminosilane-agarose hydrogel scaffolds.","authors":"V Sánchez-Vaquero, C Satriano, N Tejera-Sánchez, L González Méndez, J P García Ruiz, M Manso Silván","doi":"10.1116/1.3388182","DOIUrl":"https://doi.org/10.1116/1.3388182","url":null,"abstract":"Agarose hydrogels containing aminopropyl triethoxy silane (APTS) have been prepared and evaluated as scaffolds for adhesion and proliferation of human mesenchymal stem cells (hMSCs). The preparation of the hydrogels involved the conventional melting of agarose in water followed by addition of APTS as functional group carrier. The resulting hydrogel supports have been studied by Fourier transformed infrared spectroscopy in order to get an insight into the hybrid molecular structure. X-ray photoelectron spectroscopy has been used for the analysis of the surface chemical composition of the hydrogels. It is deduced from these data that the resulting hybrid structure presents two phases with a clear tendency toward APTS surface segregation. Moreover, the observation of the desiccated hydrogel surfaces by atomic force microscopy shows that the films acquire a filament-mesh structure for increasing APTS content, while the pure agarose supports exhibit a granular structure. As a result of such a structure, the hydrogel surfaces show a hydrophobic behavior, as determined by water contact angle measurements. The biocompatibility of such platforms is supported by adhesion-proliferation assays performed with hMSCs. It is concluded that although adhesion is lower on APTS rich scaffolds, the proliferation rate on these surfaces is higher so that total number of proliferating cells does not significantly depend on APTS content in the hydrogels.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1116/1.3388182","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40061216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Protein resistant oligo(ethylene glycol) terminated self-assembled monolayers of thiols on gold by vapor deposition in vacuum. 真空气相沉积法制备抗蛋白质低聚乙二醇自组装单硫醇层。

IF 2.1 4区医学

Biointerphases Pub Date : 2010-06-01 DOI: 10.1116/1.3407483

Laxman Kankate, Udo Werner, Andrey Turchanin, Armin Gölzhäuser, Helge Grossmann, Robert Tampé

{"title":"Protein resistant oligo(ethylene glycol) terminated self-assembled monolayers of thiols on gold by vapor deposition in vacuum.","authors":"Laxman Kankate, Udo Werner, Andrey Turchanin, Armin Gölzhäuser, Helge Grossmann, Robert Tampé","doi":"10.1116/1.3407483","DOIUrl":"https://doi.org/10.1116/1.3407483","url":null,"abstract":"Protein resistant oligo(ethylene glycol) (OEG) terminated self-assembled monolayers (SAMs) of thiols on gold are commonly used for suppression of nonspecific protein adsorption in biology and biotechnology. The standard preparation for these SAMs is the solution method (SM) that involves immersion of the gold surface in an OEG solution. Here the authors present the preparation of 11-(mercaptoundecyl)-triethylene glycol [HS(CH(2))(11)(OCH(2)CH(2))(3)OH] SAMs on gold surface by vapor deposition (VD) in vacuum. They compare the properties of SAMs prepared by VD and SM using x-ray photoelectron spectroscopy (XPS), polarization modulation infrared reflection absorption spectroscopy, and surface plasmon resonance measurements. VD and SM SAMs exhibit similar packing density and show a similar resistance to the nonspecific adsorption of various proteins (bovine serum albumin, trypsin, and myoglobin) under physiological conditions. A very high sensitivity of the OEG SAMs to x-ray radiation is found, which allows tuning their protein resistance. These results show a new path to in situ engineering, analysis, and patterning of protein resistant OEG SAMs by high vacuum and ultrahigh vacuum techniques.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1116/1.3407483","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40061217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20