Biointerphases最新文献

Interleukin 1β and lipopolysaccharides induction dictate chondrocyte morphological properties and reduce cellular roughness and adhesion energy comparatively. 白细胞介素1β和脂多糖的诱导决定了软骨细胞的形态特性，并相对降低了细胞粗糙度和粘附能。

IF 2.1 4区医学

Biointerphases Pub Date : 2022-09-30 DOI: 10.1116/6.0001986

Alia H Mallah, Mahmoud Amr, Arda Gozen, Juana Mendenhall, Bernard J Van-Wie, Nehal I Abu-Lail

{"title":"Interleukin 1β and lipopolysaccharides induction dictate chondrocyte morphological properties and reduce cellular roughness and adhesion energy comparatively.","authors":"Alia H Mallah, Mahmoud Amr, Arda Gozen, Juana Mendenhall, Bernard J Van-Wie, Nehal I Abu-Lail","doi":"10.1116/6.0001986","DOIUrl":"10.1116/6.0001986","url":null,"abstract":"Osteoarthritis (OA) is a whole joint disease marked by the degradation of the articular cartilage (AC) tissue, chronic inflammation, and bone remodeling. Upon AC's injury, proinflammatory mediators including interleukin 1β (IL1β) and lipopolysaccharides (LPS) play major roles in the onset and progression of OA. The objective of this study was to mechanistically detect and compare the effects of IL1β and LPS, separately, on the morphological and nanomechanical properties of bovine chondrocytes. Cells were seeded overnight in a full serum medium and the next day divided into three main groups: A negative control (NC) of a reduced serum medium and 10 ng/ml IL1ß or 10 ng/ml LPS-modified media. Cells were induced for 24 h. Nanomechanical properties (elastic modulus and adhesion energy) and roughness were quantified using atomic force microscopy. Nitric oxide, prostaglandin 2 (PGE2), and matrix metalloproteinases 3 (MMP3) contents; viability of cells; and extracellular matrix components were quantified. Our data revealed that viability of the cells was not affected by inflammatory induction and IL1ß induction increased PGE2. Elastic moduli of cells were similar among IL1β and NC while LPS significantly decreased the elasticity compared to NC. IL1ß induction resulted in least cellular roughness while LPS induction resulted in least adhesion energy compared to NC. Our images suggest that IL1ß and LPS inflammation affect cellular morphology with cytoskeleton rearrangements and the presence of stress fibers. Finally, our results suggest that the two investigated inflammatory mediators modulated chondrocytes' immediate responses to inflammation in variable ways.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526521/pdf/BJIOBN-000017-051001_1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40385929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tutorial on the instrumentation of sum frequency generation vibrational spectroscopy: Using a Ti:sapphire based system as an example. 和频产生振动光谱仪器教程:以Ti:蓝宝石为例。

IF 2.1 4区医学

Biointerphases Pub Date : 2022-09-07 DOI: 10.1116/6.0002007

Lujuan Yang, Wenkai Zhang, Hongtao Bian, Gang Ma

引用次数: 1

Mass spectral imaging showing the plant growth-promoting rhizobacteria's effect on the Brachypodium awn. 质谱成像显示促进植物生长的根瘤菌对短茅芒的影响。

IF 2.1 4区医学

Biointerphases Pub Date : 2022-06-23 DOI: 10.1116/6.0001949

Yuchen Zhang, Rachel Komorek, Zihua Zhu, Qiaoyun Huang, Wenli Chen, Janet Jansson, Christer Jansson, Xiao-Ying Yu

{"title":"Mass spectral imaging showing the plant growth-promoting rhizobacteria's effect on the Brachypodium awn.","authors":"Yuchen Zhang, Rachel Komorek, Zihua Zhu, Qiaoyun Huang, Wenli Chen, Janet Jansson, Christer Jansson, Xiao-Ying Yu","doi":"10.1116/6.0001949","DOIUrl":"https://doi.org/10.1116/6.0001949","url":null,"abstract":"The plant growth-promoting rhizobacteria (PGPR) on the host plant surface play a key role in biological control and pathogenic response in plant functions and growth. However, it is difficult to elucidate the PGPR effect on plants. Such information is important in biomass production and conversion. Brachypodium distachyon (Brachypodium), a genomics model for bioenergy and native grasses, was selected as a C3 plant model; and the Gram-negative Pseudomonas fluorescens SBW25 (P.) and Gram-positive Arthrobacter chlorophenolicus A6 (A.) were chosen as representative PGPR strains. The PGPRs were introduced to the Brachypodium seed's awn prior to germination, and their possible effects on the seeding and growth were studied using different modes of time-of-flight secondary ion mass spectrometry (ToF-SIMS) measurements, including a high mass-resolution spectral collection and delayed image extraction. We observed key plant metabolic products and biomarkers, such as flavonoids, phenolic compounds, fatty acids, and auxin indole-3-acetic acid in the Brachypodium awns. Furthermore, principal component analysis and two-dimensional imaging analysis reveal that the Brachypodium awns are sensitive to the PGPR, leading to chemical composition and morphology changes on the awn surface. Our results show that ToF-SIMS can be an effective tool to probe cell-to-cell interactions at the biointerface. This work provides a new approach to studying the PGPR effects on awn and shows its potential for the research of plant growth in the future.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2022-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40333685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Time-of-flight secondary ion mass spectrometry analysis of hair samples using unsupervised artificial neural network. 利用无监督人工神经网络进行头发样品的飞行时间二次离子质谱分析。

IF 2.1 4区医学

Biointerphases Pub Date : 2020-04-20 DOI: 10.1116/6.0000044

Kazuhiro Matsuda, Satoka Aoyagi

{"title":"Time-of-flight secondary ion mass spectrometry analysis of hair samples using unsupervised artificial neural network.","authors":"Kazuhiro Matsuda, Satoka Aoyagi","doi":"10.1116/6.0000044","DOIUrl":"https://doi.org/10.1116/6.0000044","url":null,"abstract":"Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is extensively employed for the structural analysis of the outermost surfaces of organic materials, including biological materials, because it provides detailed compositional information and enables high-spatial-resolution chemical mapping. In this study, a combination of TOF-SIMS and data analysis was employed to evaluate biological materials composed of numerous proteins, including unknown ones. To interpret complicated TOF-SIMS data of human hair, an autoencoder, a dimensionality reduction method based on artificial neural networks, was applied. Autoencoders can be used to perform nonlinear analysis; therefore, they are more suitable than principal component analysis (PCA) for analyzing TOF-SIMS data, which are influenced by the matrix effect. As a model sample data, the TOF-SIMS depth profile of human hair, acquired via argon gas cluster ion beam sputtering and Bi3 2+ primary ion beam, was employed. Useful information, including the characteristic distributions of amino acids and permeated surfactants on the outermost surface of the hair, was extracted from the results obtained from the autoencoder. Furthermore, the autoencoder extracted more detailed features than did PCA. Therefore, autoencoders can become a powerful tool for TOF-SIMS data analysis.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2020-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1116/6.0000044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37854156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Ar-gas cluster ion beam in ToF-SIMS for peptide and protein analysis. ToF-SIMS中ar -气簇离子束用于多肽和蛋白质分析。

IF 2.1 4区医学

Biointerphases Pub Date : 2020-04-14 DOI: 10.1116/6.0000105

Jin Gyeong Son, Sohee Yoon, Hyun Kyung Shon, Jeong Hee Moon, Sunho Joh, Tae Geol Lee

{"title":"Ar-gas cluster ion beam in ToF-SIMS for peptide and protein analysis.","authors":"Jin Gyeong Son, Sohee Yoon, Hyun Kyung Shon, Jeong Hee Moon, Sunho Joh, Tae Geol Lee","doi":"10.1116/6.0000105","DOIUrl":"https://doi.org/10.1116/6.0000105","url":null,"abstract":"Since Ar-gas cluster ion beams (Ar-GCIBs) have been introduced into time-of-flight secondary ion mass spectrometry (ToF-SIMS), there have been various attempts to analyze organic materials and biomolecules that require low-damage analysis and high sensitivity, because Ar-GCIBs allow soft ionization of large molecules such as peptides and proteins due to the low energy per atom. Here, the authors adopted the Ar-GCIB as a primary beam to detect proteins including human insulin, ubiquitin, and cytochrome C (molecular weights are 5808, 8564, and 12 327 Da, respectively). They have confirmed that the detection of the intact proteins was possible when the Ar-GCIB was used as a primary ion beam. In addition, they successfully identified each protein by analyzing the trypsin-digested peptides in myoglobin, cytochrome C, and bovine serum albumin. They also attempted on-surface enzymatic digestion to identify proteins on the surface of the Si wafer and obtained results identical to those of in-solution digestion. It is expected that the authors' on-surface digestion method can enable the application of ToF-SIMS for the analysis of proteins present in biological tissues.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2020-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1116/6.0000105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37834053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Investigations of the high-frequency dynamic properties of polymeric systems with quartz crystal resonators. 用石英晶体谐振器研究聚合物体系的高频动态特性。

IF 2.1 4区医学

Biointerphases Pub Date : 2020-04-14 DOI: 10.1116/1.5142762

Kenneth R Shull, Meredith Taghon, Qifeng Wang

引用次数: 10

Evaluation of secondary ions related to plant tissue using least absolute shrinkage and selection operator. 用最小绝对收缩和选择算子评价与植物组织有关的二次离子。

IF 2.1 4区医学

Biointerphases Pub Date : 2020-04-09 DOI: 10.1116/6.0000010

Masaru Ito, Yukari Kuga, Takayuki Yamagishi, Miya Fujita, Satoka Aoyagi

{"title":"Evaluation of secondary ions related to plant tissue using least absolute shrinkage and selection operator.","authors":"Masaru Ito, Yukari Kuga, Takayuki Yamagishi, Miya Fujita, Satoka Aoyagi","doi":"10.1116/6.0000010","DOIUrl":"https://doi.org/10.1116/6.0000010","url":null,"abstract":"With regard to life sciences, it is important to understand biological functions such as metabolic reactions at the cellular level. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) that can provide chemical mappings at 100 nm lateral resolutions is useful for obtaining three-dimensional maps of biological molecules in cells and tissues. TOF-SIMS spectra generally contain several hundred to several thousand secondary ion peaks that provide detailed chemical information. In order to manage such a large number of peaks, data analysis methods such as multivariate analysis techniques have been applied to TOF-SIMS data of complex samples. However, the interpretation of the data analysis results is sometimes still difficult, especially for biological samples. In this study, TOF-SIMS data of resin-embedded plant samples were analyzed using one of the sparse modeling methods, least absolute shrinkage and selection operator (LASSO), to directly select secondary ions related to biological structures such as cell walls and nuclei. The same sample was measured by optical microscopy and the same measurement area as TOF-SIMS was extracted in order to prepare a target image for LASSO. The same area of the TOF-SIMS and microscope data were fused to evaluate the influence of the image fusion on the TOF-SIMS spectrum information using principal component analysis. Specifically, the authors examined onion mycorrhizal root colonized with Gigaspora margarita (an arbuscular mycorrhizal fungus). The results showed that by employing this approach using LASSO, important secondary ions from biological samples were effectively selected and could be clearly distinguished from the embedding resin.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2020-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1116/6.0000010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37818374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Modified silica nanoparticle coatings: Dual antifouling effects of self-assembled quaternary ammonium and zwitterionic silanes. 改性二氧化硅纳米颗粒涂层:自组装季铵盐和两性离子硅烷的双重防污效果。

IF 2.1 4区医学

Biointerphases Pub Date : 2020-04-07 DOI: 10.1116/1.5143141

Brianna R Knowles, Pawel Wagner, Shane Maclaughlin, Michael J Higgins, Paul J Molino

{"title":"Modified silica nanoparticle coatings: Dual antifouling effects of self-assembled quaternary ammonium and zwitterionic silanes.","authors":"Brianna R Knowles, Pawel Wagner, Shane Maclaughlin, Michael J Higgins, Paul J Molino","doi":"10.1116/1.5143141","DOIUrl":"https://doi.org/10.1116/1.5143141","url":null,"abstract":"This work examines the antifouling effect of quaternary ammonium silane (QAS) grafted from coatings of silica nanoparticles (SiNPs), independently and in combination with a zwitterionic sulfobetaine (SB) silane. The binding of QAS to the SiNP coatings was monitored using quartz crystal microgravimetry with dissipation monitoring (QCM-D) under varied pH and solution concentrations. Adsorption of bovine serum albumin protein was reduced on QAS modified SiNP coatings prepared under alkaline conditions due to the proposed generation of a pseudozwitterionic interface, where the underlying SiNP surface presents an anionic charge at high pH. Significant reductions in protein binding were achieved at low functionalization concentrations and short modification times. Additionally, SiNP coatings modified with a combination of QAS and SB chemistries were investigated. Surface modifications were performed sequentially, varying silane concentration and order of addition, and monitored using QCM-D. Dual-functionalized surfaces presented enhanced resistance to protein adsorption compared to QAS or SB modified surfaces alone, even at low functionalization concentrations. The antiadhesive and antibacterial properties of functionalized surfaces were investigated by challenging the surfaces against the bacterium Escherichia coli. All dual-functionalized coatings showed equal or reduced bacterial adhesion compared to QAS and SB functionalizations alone, while coatings functionalized with high concentrations of combined chemistries reduced the adhesion of bacteria by up to 95% compared to control SiNP surfaces.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2020-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1116/1.5143141","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37812186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Investigating matrix effects of different combinations of lipids and peptides on TOF-SIMS data. 研究脂质和多肽不同组合对TOF-SIMS数据的基质效应。

IF 2.1 4区医学

Biointerphases Pub Date : 2020-04-02 DOI: 10.1116/6.0000036

Keisuke Mizomichi, Takayuki Yamagishi, Tomoko Kawashima, Michael Dürr, Satoka Aoyagi

{"title":"Investigating matrix effects of different combinations of lipids and peptides on TOF-SIMS data.","authors":"Keisuke Mizomichi, Takayuki Yamagishi, Tomoko Kawashima, Michael Dürr, Satoka Aoyagi","doi":"10.1116/6.0000036","DOIUrl":"https://doi.org/10.1116/6.0000036","url":null,"abstract":"Matrix effects, which cause a change in ion intensity, occur in mass spectrometry methods including time-of-flight secondary ion mass spectrometry (TOF-SIMS). Matrix effects often cause large issues in quantitative analysis because secondary ions related to a particular molecule could be dramatically enhanced or suppressed regardless of the concentration. To investigate matrix effects in biological samples, the authors evaluated mixed lipid {POPC [1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine, molecular weight (MW) 759.6]}, peptide [leu-enkephalin, neo-leu-enkephalin (amino acid sequence: YAGFL, MW 569.3), and neo-angiotensin II (amino acid sequence: DRVYIHAF, MW 1019.5)] samples. Matrix effect features were investigated by analyzing the concentration dependence of secondary ions in lipid-peptide mixed samples to develop a method that enables quantitative analysis using TOF-SIMS. Matrix effects depended on the lipid-peptide combination. Interestingly, some secondary ions possessed an intensity that was highly dependent on concentration.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2020-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1116/6.0000036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37797152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human plasma protein adsorption to elastinlike polypeptide nanoparticles. 人血浆蛋白对弹性蛋白样多肽纳米颗粒的吸附。

IF 2.1 4区医学

Biointerphases Pub Date : 2020-03-30 DOI: 10.1116/6.0000027

Markian S Bahniuk, Abdullah K Alshememry, Larry D Unsworth

{"title":"Human plasma protein adsorption to elastinlike polypeptide nanoparticles.","authors":"Markian S Bahniuk, Abdullah K Alshememry, Larry D Unsworth","doi":"10.1116/6.0000027","DOIUrl":"https://doi.org/10.1116/6.0000027","url":null,"abstract":"Elastin-like polypeptides (ELPs) are being developed for numerous biomedical applications. There is a limited understanding of ELP biocompatibility, with conflicting results in the literature. Protein adsorption is the fate determining event for blood-contacting biomaterials. The aim of this study is to elucidate the biocompatibility of ELP-based nanoparticles by examining the adsorbed proteome from platelet poor human plasma as a function of the physicochemical properties of these nanoparticles: diameter, amino acid hydrophobicity, and chain length. It was found that all ELP constructs had adsorbed an extremely large amount of albumin and high levels of immunoglobulin G and activated complement factor 3. Variations in the compositions of the proteomes across the eight nanoparticle systems studied were observed for plasminogen, fibronectin, activated fibrinogen, and coagulation modulating antithrombin and alpha2 macroglobulin. Plasma clotting experiments showed that ELP-based nanoparticles slightly inhibited normal blood clotting, with shorter and/or more hydrophilic constructs showing a greater difference from the control than longer or more hydrophobic constructs. These results indicate that ELP nanoparticles, regardless of chain length, particle diameter, or amino acid hydrophobicity, may have the potential to stimulate a humoral immune response via immunoglobulin G and activated complement factor 3 despite the large amounts of albumin adsorbed at the blood-material interface.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2020-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1116/6.0000027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37784351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7