研究脂质和多肽不同组合对TOF-SIMS数据的基质效应。

IF 2.1 4区医学 Q2 Physics and Astronomy

Biointerphases Pub Date : 2020-04-02 DOI:10.1116/6.0000036

Keisuke Mizomichi, Takayuki Yamagishi, Tomoko Kawashima, Michael Dürr, Satoka Aoyagi

{"title":"研究脂质和多肽不同组合对TOF-SIMS数据的基质效应。","authors":"Keisuke Mizomichi, Takayuki Yamagishi, Tomoko Kawashima, Michael Dürr, Satoka Aoyagi","doi":"10.1116/6.0000036","DOIUrl":null,"url":null,"abstract":"Matrix effects, which cause a change in ion intensity, occur in mass spectrometry methods including time-of-flight secondary ion mass spectrometry (TOF-SIMS). Matrix effects often cause large issues in quantitative analysis because secondary ions related to a particular molecule could be dramatically enhanced or suppressed regardless of the concentration. To investigate matrix effects in biological samples, the authors evaluated mixed lipid {POPC [1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine, molecular weight (MW) 759.6]}, peptide [leu-enkephalin, neo-leu-enkephalin (amino acid sequence: YAGFL, MW 569.3), and neo-angiotensin II (amino acid sequence: DRVYIHAF, MW 1019.5)] samples. Matrix effect features were investigated by analyzing the concentration dependence of secondary ions in lipid-peptide mixed samples to develop a method that enables quantitative analysis using TOF-SIMS. Matrix effects depended on the lipid-peptide combination. Interestingly, some secondary ions possessed an intensity that was highly dependent on concentration.","PeriodicalId":49232,"journal":{"name":"Biointerphases","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2020-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1116/6.0000036","citationCount":"0","resultStr":"{\"title\":\"Investigating matrix effects of different combinations of lipids and peptides on TOF-SIMS data.\",\"authors\":\"Keisuke Mizomichi, Takayuki Yamagishi, Tomoko Kawashima, Michael Dürr, Satoka Aoyagi\",\"doi\":\"10.1116/6.0000036\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Matrix effects, which cause a change in ion intensity, occur in mass spectrometry methods including time-of-flight secondary ion mass spectrometry (TOF-SIMS). Matrix effects often cause large issues in quantitative analysis because secondary ions related to a particular molecule could be dramatically enhanced or suppressed regardless of the concentration. To investigate matrix effects in biological samples, the authors evaluated mixed lipid {POPC [1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine, molecular weight (MW) 759.6]}, peptide [leu-enkephalin, neo-leu-enkephalin (amino acid sequence: YAGFL, MW 569.3), and neo-angiotensin II (amino acid sequence: DRVYIHAF, MW 1019.5)] samples. Matrix effect features were investigated by analyzing the concentration dependence of secondary ions in lipid-peptide mixed samples to develop a method that enables quantitative analysis using TOF-SIMS. Matrix effects depended on the lipid-peptide combination. Interestingly, some secondary ions possessed an intensity that was highly dependent on concentration.\",\"PeriodicalId\":49232,\"journal\":{\"name\":\"Biointerphases\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2020-04-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1116/6.0000036\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biointerphases\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1116/6.0000036\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Physics and Astronomy\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biointerphases","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1116/6.0000036","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Physics and Astronomy","Score":null,"Total":0}

引用次数: 0

摘要

导致离子强度变化的基质效应发生在质谱法中，包括飞行时间二次离子质谱法(TOF-SIMS)。基质效应在定量分析中经常引起大问题，因为与特定分子相关的二次离子无论浓度如何都可能显著增强或抑制。为了研究基质在生物样品中的作用，作者评估了混合脂质{POPC[1-棕榈酰-2-油酰- n-甘油-3-磷脂酰胆碱，分子量(MW) 759.6]}、肽[亮氨酸脑啡肽、新亮氨酸脑啡肽(氨基酸序列:YAGFL, MW 569.3)和新血管紧张素II(氨基酸序列:DRVYIHAF, MW 1019.5)]样品。通过分析脂质-肽混合样品中二次离子的浓度依赖性，研究基质效应特征，建立了一种使用TOF-SIMS进行定量分析的方法。基质效应取决于脂质-肽的组合。有趣的是，一些二次离子具有高度依赖于浓度的强度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Investigating matrix effects of different combinations of lipids and peptides on TOF-SIMS data.

Matrix effects, which cause a change in ion intensity, occur in mass spectrometry methods including time-of-flight secondary ion mass spectrometry (TOF-SIMS). Matrix effects often cause large issues in quantitative analysis because secondary ions related to a particular molecule could be dramatically enhanced or suppressed regardless of the concentration. To investigate matrix effects in biological samples, the authors evaluated mixed lipid {POPC [1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine, molecular weight (MW) 759.6]}, peptide [leu-enkephalin, neo-leu-enkephalin (amino acid sequence: YAGFL, MW 569.3), and neo-angiotensin II (amino acid sequence: DRVYIHAF, MW 1019.5)] samples. Matrix effect features were investigated by analyzing the concentration dependence of secondary ions in lipid-peptide mixed samples to develop a method that enables quantitative analysis using TOF-SIMS. Matrix effects depended on the lipid-peptide combination. Interestingly, some secondary ions possessed an intensity that was highly dependent on concentration.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biointerphases BIOPHYSICS-MATERIALS SCIENCE, BIOMATERIALS

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.