Cell Journal最新文献

{"title":"Feasibility and Safety of IL-15-Activated CD56+ Cell Therapy in High-Risk Acute Myeloid Leukemia after Hematopoietic Stem Cell Transplantation: Phase I Clinical Trial.","authors":"Shiva Eskandarian, Amirhossein Izadpanah, Mozhdeh Mohammadian, Mehdi Bakhtiyaridovvmbaygi, Mehrshad Seresht-Ahmadi, Sahar Parkhideh, Elham Roshandel, Ahmad Gharehbaghian, Abbas Hajifathali","doi":"10.22074/cellj.2025.2057631.1827","DOIUrl":"https://doi.org/10.22074/cellj.2025.2057631.1827","url":null,"abstract":"<p><strong>Objective: </strong>Allogeneic hematopoietic stem cell transplantation (allo-HSCT) remains the most effective treatment for patients with high-risk acute myeloid leukemia (AML), but relapse remains a major challenge. Immunotherapy is considered a promising approach for reducing the risk of relapse. Natural killer (NK) cells exert cytotoxic effects against malignant cells, and their activation with interleukin-15 (IL-15) enhances anti-leukemic immune responses. This study evaluated the safety and feasibility of <i>in vitro</i> IL-15 activation of CD56⁺ cells and assessed the safety of their infusion in AML patients following allo-HSCT.</p><p><strong>Materials and methods: </strong>In this phase I clinical trial study, CD56⁺ cells were isolated from non-mobilized donors using a one-step CD56 enrichment protocol to obtain NK and NKT cells. CD56⁺ cells were activated by overnight incubation with IL-15. A cytotoxicity assay was performed against K562 cells. Three escalating doses of CD56⁺ cells consisting of 1×10⁶, 3×10⁶, and 5×10⁶ cells/kg of patient bodyweight were infused to the three patients on days +7, +14, and +21 post-allo-HSCT. Patients were observed during and up to 4 hours after the infusion for immediate adverse events and were monitored for 21 days to detect delayed events.</p><p><strong>Results: </strong>Activation with IL-15 increased the expression of activating receptors, including CD25, CD69, NKp30, NKp46, and NKG2D decreased the expression of the inhibitory receptor NKG2A. The cytotoxicity of IL-15-activated CD56⁺ cells was higher than that of non-activated CD56⁺ cells. This method was safe and no intervention-related complications were observed.</p><p><strong>Conclusion: </strong>We identified a feasible method to activate CD56⁺ cells and evaluate the safety of their infusion in patients. Despite the value of activating CD56⁺ cells with IL-15, further studies with larger sample sizes are needed to confirm and validate the current hypothesi (registration number: IRCT20230801058996N2).</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2026-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146203530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal Prediction of Umbilical Cord Blood CD34⁺ Adequacy and Identifying Influential Factors via An Ensemble Machine Learning and TOPSIS Ranking: A Retrospective Study.","authors":"Amir Hosein Esmaielpour, Mariam Ameli, Ashkhan Mozdgir, Orod Ahmadi, Morteza Zarabi","doi":"10.22074/cellj.2025.2064741.1878","DOIUrl":"https://doi.org/10.22074/cellj.2025.2064741.1878","url":null,"abstract":"<p><strong>Objective: </strong>Prenatal prediction of CD34⁺ adequacy supports cord-blood banking by reducing expenses on low-yield units and reserving capacity for clinically promising grafts. We have developed and evaluated a prenatal machine learning model that predicts whether an umbilical cord blood (UCB) unit will meet a clinically supported adequacy threshold (≥1.5×10⁵ CD34⁺ cells/kg recipient) before collection for single-unit grafts.</p><p><strong>Materials and methods: </strong>In this retrospective study, we analysed 126,406 records from the Royan Stem Cell Technology Company (RSCT; Tehran, Iran), which included routinely available maternal, neonatal, and family-history variables. A pipeline of imputation (IterativeImputer numeric; SimpleImputer+OrdinalEncoder categorical), feature selection (Extra Trees), and hyperparameter tuning using Bayesian optimisation with model training/evaluation was performed within cross-validation folds. Decision Tree (DT), K-Nearest Neighbours (KNN), Random Forest (RF), Support Vector Machine (SVM), and Multilayer Perceptron (MLP) classifiers were tuned via Bayesian optimisation. Models were ranked by the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS). Majority voting (MV) ensembles were constructed from the top-k models. Model interpretability used SHapley Additive exPlanations (SHAP).</p><p><strong>Results: </strong>The MV (top-4: RF, KNN, DT, MLP) ensemble achieved an area under the receiver operating characteristic curve (ROC-AUC)=0.808 and an area under the precision-recall curve (PR-AUC)=0.744 on the held-out test set, with an accuracy=0.757, precision=0.726, recall/sensitivity=0.804, F1=0.762, specificity=0.716, and Brier score=0.181. SHAP highlighted history of hepatitis C, birth place, hyperthyroidism, history of anaemia, oral fungus, and rheumatism among the most influential features.</p><p><strong>Conclusion: </strong>Prenatal prediction of UCB CD34⁺ adequacy using an interpretable MV ensemble is feasible and accurate to support pre-collection triage and can potentially improve banking efficiency. The resultant model offers a non-invasive tool to enhance the efficiency of cord blood banking by prioritising units with higher transplantation potential.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2026-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146203590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Exercise-Based Interventions on HbA1c in Adults with Type 1 Diabetes: A Systematic Review and Meta-Analysis.","authors":"Fatemeh Malakoutinia, Azadeh Ghaheri, Samira Vesali, Azam Kouhkan, Yaser Tahamtani, Masoud Rahmati, Roya Hosseini","doi":"10.22074/cellj.2025.2050534.1775","DOIUrl":"https://doi.org/10.22074/cellj.2025.2050534.1775","url":null,"abstract":"<p><strong>Objective: </strong>This study intends to determine whether exercise-based interventions improve haemoglobin A1c (HbA1c) levels in adults with type 1 diabetes (T1D) and examines how different types of exercise influence this association.</p><p><strong>Materials and methods: </strong>A search of the appropriate electronic databases revealed 18 eligible studies from initial 685 records. The effect of exercise training on HbA1c was examined in two parts: i. Exercise training intervention compared to a T1D control group (between-group analysis) and ii. Pre/post comparison of exercise training intervention on T1D patients (within-group analysis).</p><p><strong>Results: </strong>The between-group results showed that exercise training had a statistically significant reduction in HbA1c [mean difference (MD), confidence interval (CI)]: -0.39 (-0.64, -0.15), P=0.002, I²=0%). Subgroup analysis based on the types of exercise training showed a statistically significant effect of high-intensity interval training (HIIT) on HbA1c levels in both the within-group analyses [MD: -0.24 (-0.45, -0.02), P=0.03, I²=0%] and between-group analyses [MD: -0.39 (-0.64, -0.15), P=0.002, I²=0%].</p><p><strong>Conclusion: </strong>The meta-analysis and meta-regression results suggest that HIIT may be a supportive intervention in T1D patients; however, there was insufficient evidence to confirm these findings. Data from more randomised controlled trials (RCTs) are needed to compare the effect of different types of exercise on HbA1c levels in T1D patients [registration: PROSPERO (CRD42023414580)].</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-14"},"PeriodicalIF":1.7,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146203578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR-Based Single-Nucleotide Editing of <i>PIK3CA</i> c.3140A>G (p.His1047Arg) in MCF7 Breast Cancer Cells Enhances Proliferative Potential.","authors":"Omid Dehqani Dashtabi, Azin Amoozeidi, Saman Hosseinkhani, Seyed Javad Mowla","doi":"10.22074/cellj.2025.2055578.1813","DOIUrl":"https://doi.org/10.22074/cellj.2025.2055578.1813","url":null,"abstract":"<p><strong>Objective: </strong>The PI3K/Akt signaling pathway plays a central role in regulating cell growth, survival, and metabolism, and its dysregulation is a hallmark of many cancers. The PIK3CA gene, which encodes the alpha catalytic subunit of PI3K, is altered in approximately 30% of breast cancers. Among its mutations, c.3140A>G (p.His1047Arg) in the kinase domain is the most prevalent, producing a constitutively active enzyme with oncogenic potential. Here, we engineered a population of MCF7 cells carrying the <i>PIK3CA</i> c.3140A>G mutation using CRISPR-Cas9 with precise single-nucleotide editing, and evaluated its impact on cellular characteristics.</p><p><strong>Materials and methods: </strong>In this experimental study, nearly homogeneous populations of <i>PIK3CA</i> H1047R mutant MCF7 cells were generated using CRISPR-Cas9-mediated genome editing followed by hierarchical single-cell isolation. Editing efficiency was validated through allele-specific polymerase chain reaction (PCR) and multiple rounds of Sanger sequencing. Cell cycle distribution and proliferation were analyzed using flow cytometry and cell count assays, respectively. Gene expression changes were assessed by quantitative real-time PCR to evaluate the mutation's impact on cell cycle-related genes.</p><p><strong>Results: </strong>Tracking of insertions, deletions, and recombination events (TIDER) analysis showed approximately 60% homology-directed repair (HDR) efficiency in the edited population. Flow cytometry revealed a 5% increase in the G2/M cell population in the edited clone compared with unedited controls (P<0.001). Proliferation assays demonstrated significantly accelerated growth (1.30 fold) under low fetal bovine serum (FBS) conditions (P=0.029). Quantitative real-time PCR confirmed upregulation of cell cycle-promoting genes, with <i>CCND1</i> and MYC expression increasing by 1.62-fold (P<0.001) and 1.23-fold (P<0.001), respectively, relative to controls.</p><p><strong>Conclusion: </strong>The genetically edited cell lines represent robust and well-defined experimental models that enable direct assessment of the functional consequences of oncogenic driver mutations on cellular behavior and signaling pathways. Our findings demonstrate that targeted genetic alterations induce measurable changes in proliferation, cell-cycle regulation, and gene expression, thereby providing mechanistic insight into tumorigenesis and the specific contribution of driver mutations to cancer-related cellular phenotypes.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-12"},"PeriodicalIF":1.7,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146203518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperglycemia Increased The Expression of CALCRL G Protein-Coupled Receptor in Monocytes from Diabetic Patients.","authors":"Seyedeh Zohreh Azarshin, Maral Marashi, Zahra Abedi Kichi, Mohammad Ali Boroumand, Mehrdad Behmanesh","doi":"10.22074/cellj.2025.2059066.1843","DOIUrl":"https://doi.org/10.22074/cellj.2025.2059066.1843","url":null,"abstract":"<p><strong>Objective: </strong>Diabetes is a metabolic disorder with two common categories of complications: microvascular and macrovascular. Cardiovascular diseases (CVDs), which are linked to inflammation in the blood vessels, are the most important complications of diabetes. G protein-coupled receptors (GPCRs) play a crucial role in initiating these inflammatory responses. The aim of this study is to identify GPCRs that may respond to high blood glucose in monocyte cells and have an effect on regulating vascular inflammation.</p><p><strong>Materials and methods: </strong>In this in-silico and experimental study, first, RNA sequencing (RNAseq) data from monocytes from the blood of diabetic patients were analyzed to find the GPCR most responsive to high blood sugar. Next, the expression correlation of the candidate GPCR with inflammatory genes was calculated, and expression changes in THP-1 cells under hyperglycemia were evaluated via quantitative polymerase chain reaction (qPCR). Finally, the effect of the candidate GPCR on down-regulation of <i>NF-kB mRNA</i> expression, generation of reactive oxygen species (ROS), and caspase activity was evaluated.</p><p><strong>Results: </strong>The RNAseq analysis showed that expression of Calcitonin receptor-like (<i>CALCRL</i>), one of the GPCR candidates, increased in the monocytes of patients with diabetes compared to healthy individuals. The qPCR result also indicated that expression of the <i>CALCRL</i> gene increased in THP-1 cells treated with 25 mM D-glucose. Correlation analyses demonstrated that increased expression of <i>CALCRL</i> is associated with inflammatory pathways, cytokine production and secretion, and the cell migration process. Finally, our data indicated that <i>CALCRL</i> downregulation decreased inflammation related to hyperglycemia, including <i>NF-kB mRNA</i> expression, ROS production, and caspase 3/7 activity.</p><p><strong>Conclusion: </strong>Hyperglycemia increased the expression of <i>CALCRL</i> GPCR in the monocytes of diabetic patients. The CALCRL receptor may play a key role in regulating vascular inflammation in hyperglycemic conditions. Targeting CALCRL may control inflammation in blood cells.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2025-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145795143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Vesicles Derived from Human Embryonic Stem CellsReduce The Stemness Properties of Cancer Cell Lines.","authors":"Maryam Akhoundi, Mehran Rezaei Larijani, Reza Azmakan, Maryam Saber, Atefeh Safarpour, Niloofar Sodeifi, Marzieh Ebrahimi, Seyedeh-Nafiseh Hassani","doi":"10.22074/cellj.2025.2049975.1768","DOIUrl":"https://doi.org/10.22074/cellj.2025.2049975.1768","url":null,"abstract":"<p><strong>Objective: </strong>Aggressive cancer cells exhibit many phenotypic and functional similarities to embryonic stem cells (ESCs), including characteristics such as self-renewal, differentiation flexibility, and specific gene expression signatures. While the ESC microenvironment precisely regulates proliferation to balance self-renewal and differentiation, it remains unclear whether tumor cells can respond to these regulatory signals to suppress their tumorigenic phenotype. Extracellular vesicles (EVs) released by human ESCs (hESCs) are known to exert paracrine effects, making them prime candidates for carrying these regulatory signals. The aim of this study was to determine if hESC-EVs suppress the tumorigenic phenotype of aggressive cancer cells by affecting their proliferative, invasive, and stem cell properties.</p><p><strong>Materials and methods: </strong>In this experimental study, EVs were isolated from hESC-conditioned medium (hESC-CM) and thoroughly characterized. Aggressive breast and melanoma cancer cell lines were treated with the isolated hESCderived EVs (hESC-EVs). The resulting effects on cell growth (proliferation), invasion, stem cell properties (stemness), and overall tumorigenesis were evaluated <i>in vitro</i> and <i>in vivo</i>. Expression levels of key pluripotency markers were also assessed in the treated cancer cells.</p><p><strong>Results: </strong>The treatment of the aggressive cancer cell lines with hESC-EVs resulted in a significant reduction in proliferation, invasion, stemness, and tumorigenesis across both <i>in vitro</i> and <i>in vivo</i> models. Mechanistically, exposure to hESC-EVs led to the clear downregulation of pluripotency markers within the recipient cancer cells.</p><p><strong>Conclusion: </strong>These findings suggest that hESC-EVs transmit powerful regulatory signals capable of inducing the loss of stem cell characteristics in aggressive cancer cells. This mechanism offers a potential pathway for reprogramming highly malignant tumor cells toward a more differentiated and less aggressive phenotype, suggesting a novel strategy for anti-cancer therapy.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-15"},"PeriodicalIF":1.7,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145758096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm Selection through Microvesicle-Mediated ChemotaxisInduced by Follicular Fluid and Cumulus Cells.","authors":"Tohid Rezaei Topraggaleh, Amir Fattahi, Ehsan Dadkhah, Behrooz Niknafs","doi":"10.22074/cellj.2025.2047757.1743","DOIUrl":"https://doi.org/10.22074/cellj.2025.2047757.1743","url":null,"abstract":"<p><strong>Objective: </strong>Selecting the most competent sperm is crucial for improving the success of intracytoplasmic sperm injection (ICSI). Chemotaxis-based sperm selection may facilitate the selection of spermatozoa with superior motility and fertilization capability. This study aimed to evaluate sperm selection based on chemotaxis induced by microvesicles (MVs) derived from follicular fluid (FF) and cumulus cells (CCs) using a simple microfluidic device.</p><p><strong>Materials and methods: </strong>In this experimental study, FF and CCs were collected during ovum pick-up from 10 women undergoing ICSI. MVs from both FF and CCs were isolated using the Exocib kit and characterized for particle size, polydispersity index, zeta potential, morphology, and protein content. To establish a chemoattractant gradient, MVs from FF and CCs, as well as progesterone, were incorporated into agar plates. Nine experimental groups were studied: FF- derived MVs (FF-MV, 5, 25, and 50 μg/ml), CC-derived MV (CC-MV, 5, 25, and 50 μg/ml), FF (50 μg/ml), progesterone (0.03 μM), and a control (agar without chemoattractant). Sperm selection was performed in a microfluidic system using semen samples from 20 normozoospermic men. Sperm concentration, motility, progressive motility, viability, and DNA integrity were analyzed following chemotaxis-based selection.</p><p><strong>Results: </strong>The isolated FF-and CC-derived MVs exhibited spherical morphology, a negative zeta potential, and were polydispersed. The progesterone-containing plates showed sustained release. No significant differences were observed between the CC-MV and control groups in sperm parameters (P=0.630). Plates supplemented with 50 μg/mL of FF-MV markedly improved sperm concentration (P=0.002), progressive motility (P=0.010), and DNA integrity (P=0.001) compared to the control group.</p><p><strong>Conclusion: </strong>FF-MVs, particularly at 50 μg/ml, possess chemoattractant properties and could selectively enrich sperm populations with improved functional parameters. These findings highlight the potential of FF-MVs as a novel tool for high-quality sperm selection in the ICSI procedure.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145745467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Neuroinflammatory Potential of Drospirenone in A TLR4-Driven <i>In Vitro</i> Model of Neuropathic Pain.","authors":"Leila Taheran, Hakimeh Zali, Mohammad Ajoudanian, Mir-Shahram Safari, Samira Rajaei, Zahra Hajimahdi, Ali Dabbagh","doi":"10.22074/cellj.2025.2049252.1758","DOIUrl":"https://doi.org/10.22074/cellj.2025.2049252.1758","url":null,"abstract":"<p><strong>Objective: </strong>Neuroinflammation plays a crucial role in neuropathic pain, of which toll-like receptor 4 (TLR4) is a key mediator. Virtual screening identified Drospirenone as a potential TLR4 inhibitor. This study aims to evaluate the <i>in vitro</i> effects of Drospirenone on TLR4 signalling in U87-MG astrocytoma cells under different inflammatory conditions.</p><p><strong>Materials and methods: </strong>In this experimental study, three treatment approaches with Drospirenone were employed to model various stages of neuroinflammation: co-incubation with lipopolysaccharides (LPS) to assess preventive effects; treatment after inflammation to evaluate its impact on sustained inflammation; and delayed treatment after LPS removal to investigate its role in reducing persistent inflammation. Expression levels of TLR4, myeloid differentiation primary response 88 (MyD88), nuclear factor-κB (NF-κB) p65, and interleukin-1 beta (IL-1β) were assessed by Western blotting, while nitric oxide (NO) secretion was measured by ELISA.</p><p><strong>Results: </strong>The potential ability of Drospirenone to inhibit early-stage inflammation was shown by significant reductions in TLR4, MyD88, NF-κBp65, IL-1β, and NO. Treatment after inflammation showed that Drospirenone reduced NO secretion but did not significantly affect TLR4 and other inflammatory markers, which could indicate its potential efficacy in controlling sustained inflammation. In the delayed treatment approach, inflammation persisted after LPS removal, and Drospirenone did not return the inflammatory state to baseline.</p><p><strong>Conclusion: </strong>Drospirenone may exhibit potential as a prophylactic agent <i>in vitro</i> during the early phases of neuroinflammation, though its efficacy appears limited in models of chronic or prolonged inflammation. These preliminary findings require <i>in vivo</i> validation, and future studies could explore possible synergistic effects with other treatments or alternative dosing strategies for neuropathic pain management.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2025-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146068064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Commercial Kit versus In-House Protocol for Assessing Endodermal Differentiation Potential in Human Embryonic Stem Cells.","authors":"Mehran Rezaei-Larijani, Mahsa Valizadeh, Zahra Ghezelayagh, Seyedeh-Nafiseh Hassani, Mohammad Kazemi Ashtiani, Azadeh Moradmand, Najmeh Sadat Masoudi, Bagher Larijani, Yaser Tahamtani, Hamid Reza Aghayan","doi":"10.22074/cellj.2025.2056939.1819","DOIUrl":"https://doi.org/10.22074/cellj.2025.2056939.1819","url":null,"abstract":"<p><strong>Objective: </strong>Human embryonic stem cells (hESCs) have the capacity to differentiate into all cell lineages, making them invaluable for cell-based therapies. However, the ability of individual hESC lines to generate the three embryonic germ layers varies. Selecting a cell line with a high propensity for definitive endoderm (DE) differentiation is crucial for producing pancreatic progenitors, hepatocytes, and other endodermal derivatives. Direct and spontaneous differentiation are two commonly used methods to assess hESC differentiation potential; both are complex and time-consuming. This study evaluates a commercially available differentiation kit as a simple and reproducible screening assay to predict the DE differentiation potential of various hESC lines.</p><p><strong>Materials and methods: </strong>In this experimental study, three hESC lines obtained from the Royan Institute Stem Cell Bank (Tehran, Iran) were used. Pluripotency was confirmed by evaluating cell morphology, OCT4 protein expression, and alkaline phosphatase staining. Early-stage lineage differentiation was compared between the StemMACS™ Trilineage kit and a previously established DE protocol. Differentiation outcomes were assessed using gene and protein expression analyses, including flow cytometry, quantitative real-time polymerase chain reaction (qRT-PCR), and immunostaining.</p><p><strong>Results: </strong>The three hESC lines exhibited varying capacities for DE differentiation. Flow cytometry analysis of the surface marker CXCR4 showed differentiation efficiencies of 70% for RH2, compared with 35% and 40% for RH5 and RH6, respectively. The superior efficiency of RH2 was consistent across both differentiation protocols, as evidenced by its strong CXCR4 expression using the in-house method.</p><p><strong>Conclusion: </strong>hESC lines exhibit variable capacities for differentiation. Commercial kit-based screening provides a simple and reliable method to identify suitable hESC lines for generating endoderm-derived cells.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2025-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145551509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Expression of Long Non-Coding RNAs MALAT1, ANRIL and MEG3 in Radiation-Exposed Breast Cancer.","authors":"Zahra Haghshenas, Hossein Mozdarani","doi":"10.22074/cellj.2025.2041844.1677","DOIUrl":"https://doi.org/10.22074/cellj.2025.2041844.1677","url":null,"abstract":"<p><strong>Objective: </strong>Breast cancer remains one of the leading causes of mortality among women worldwide. Approximately 80% of patients receive radiotherapy (RT) as part of their treatment. Numerous efforts have been made to distinguish radiosensitive from radioresistant breast tumors. Long non-coding RNAs (lncRNAs) have increasingly been recognized as crucial mediators of cellular responses to radiation among the wide range of biomarkers studied. This study explored the radiation-induced expression changes of three apoptosis-associated lncRNAs-<i>MEG3, MALAT1,</i> and <i>ANRIL</i>-in breast cancer.</p><p><strong>Materials and methods: </strong>In this experimental study, two established human breast cancer cell lines, MCF-7 and MDAMB- 231, were cultured in Dulbecco's Modified Eagle Medium (DMEM) and irradiated with X-rays at doses ranging from 0 to 8 Gy. The expression levels of the lncRNAs <i>ANRIL, MALAT1</i> and <i>MEG3</i> were quantified by real-time polymerase chain reaction (PCR). Cell viability in irradiated MCF-7 and MDA-MB-231 cells was evaluated using the MTT colorimetric assay.</p><p><strong>Results: </strong>Following radiation exposure, the expression levels of <i>ANRIL</i> and <i>MEG3</i> were significantly decreased (P=0.004) in both MCF-7 and MDA-MB-231 cell lines, whereas <i>MALAT1</i> expression was significantly increased (P=0.006) only in MDA-MB-231 cells. In addition, cell viability in both MCF-7 and MDA-MB-231 lines declined after irradiation.</p><p><strong>Conclusion: </strong>Our findings indicate that radiation exposure increases <i>MALAT1</i> expression while decreasing <i>ANRIL</i> and <i>MEG3</i> levels, associated with reduced cell viability .These results suggest a potential role for these lncRNAs in radiation-induced cell death.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"27 1","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2025-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145551493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}