Brazilian Journal of Otorhinolaryngology最新文献

{"title":"Functional outcomes of otosclerosis surgery","authors":"Nadia Romdhane,&nbsp;Dorra Chiboub,&nbsp;Amal Nessib,&nbsp;Ameni Amri,&nbsp;Ines Hariga,&nbsp;Chiraz Chaouch Mbarek","doi":"10.1016/j.bjorl.2025.101679","DOIUrl":"10.1016/j.bjorl.2025.101679","url":null,"abstract":"<div><h3>Objectives</h3><div>The objectives of this work were to evaluate the results of otosclerosis surgery and to study the predictors of its functional success.</div></div><div><h3>Methods</h3><div>Our study was retrospective on 322 patients, equivalent to 418 ears operated in the otolaryngology and head and neck surgery department, from January 2000 to December 2020.</div></div><div><h3>Results</h3><div>Hearing success was noted in 89.5% of patients considering the Postoperative Residual Air Bone Gap (PRABG) ≤10 dB criterion; in 94.3% of patients according to the conductive gain ≥20 dB criterion; in 96.4% of patients based on the improvement of cochlear reserve criterion; in 88.8% according to the Air Bone Gap improvement (ABGi) ≥70% criterion and in 81.8% considering all these criteria. The short, medium and long-term postoperative tonal audiometries could be superimposed, reflecting the stability of the hearing results. A regression of tinnitus has been reported in 79.2% of cases. The independent predictors of hearing success were the Aubry's audiometric stages I and II, mean preoperative air curve &gt;40 dB and platinotomy. The independent predictors of tinnitus regression retained were male sex, no exposure to loud noise and Veillon radiological stages I and II.</div></div><div><h3>Conclusion</h3><div>The identification of factors influencing the functional results of surgery allows a better selection of candidates for the intervention and more relevant information for patients.</div></div><div><h3>Level of evidence</h3><div>Level 3: Non-randomized controlled cohort or follow-up study.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 6","pages":"Article 101679"},"PeriodicalIF":1.7,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of ApoE and Spp1 in the cochlea and auditory cortex of age-related hearing loss mice","authors":"Yingxue Yuan ,&nbsp;Junhong Zhang ,&nbsp;Jingyi Zhao ,&nbsp;Xiru Zhang ,&nbsp;Zhixin Cao","doi":"10.1016/j.bjorl.2025.101674","DOIUrl":"10.1016/j.bjorl.2025.101674","url":null,"abstract":"<div><h3>Objective</h3><div>Screen for differential proteins in the cochlea of mice associated with Age-Related Hearing Loss (ARHL), analyze and validate the expression of specific differential proteins and genes in the cochlea and auditory cortex of ARHL mice, and preliminarily explore their potential mechanisms of action.</div></div><div><h3>Methods</h3><div>ABR (Auditory Brainstem Response) hearing tests were conducted to select 15-month-old C57BL/6 mice with significantly decreased hearing as the experimental group and 2-month-old mice with normal hearing as the control group. Cochleae were dissected, and unlabeled quantitative proteomics was employed to identify and analyze differentially expressed proteins in the inner ear of the two groups of mice. Key node proteins were selected via the STRING database and Cytoscape analysis. The expression of two selected proteins, ApoE and Spp1, in the cochlea was detected using qRT-PCR, Western blot, and immunofluorescence techniques, and their expression in the auditory cortex of the brain was further explored.</div></div><div><h3>Results</h3><div>Label-free quantitative proteomics identified 115 differentially expressed proteins in the cochlea of 15-month-old ARHL mice compared to 2-month-old hearing-normal mice, including 42 upregulated and 73 downregulated proteins. GO and KEGG enrichment analyses revealed significant enrichment of differentially expressed proteins in functions and signaling pathways associated with neurodegenerative diseases and neurotransmission. Protein-Protein Interaction (PPI) analysis using the STRING database and Cytoscape selection identified ApoE and Spp1 as key hub proteins. Validation via qRT-PCR, Western blot, and immunofluorescence demonstrated that both ApoE and Spp1 were highly expressed in the cochlea and auditory cortex of the ARHL mice compare to 2-month-old hearing-normal mice.</div></div><div><h3>Conclusion</h3><div>ApoE and Spp1 are upregulated in the cochlea of ARHL mice, particularly in spiral ganglion neurons, and in the auditory cortex, suggesting their potential involvement in the pathogenesis and progression of ARHL through the modulation of auditory neural conduction systems.</div></div><div><h3>Level of evidence</h3><div>Level 2.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 6","pages":"Article 101674"},"PeriodicalIF":1.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of tinnitus with obstructive sleep apnea and rapid eye movement-related obstructive sleep apnea in similar hearing threshold groups","authors":"Sun Choi ,&nbsp;Seok Hyun Park ,&nbsp;Su Yeon Kim ,&nbsp;Su Kyoung Park ,&nbsp;Sang Chul Park ,&nbsp;Jiwon Chang","doi":"10.1016/j.bjorl.2025.101661","DOIUrl":"10.1016/j.bjorl.2025.101661","url":null,"abstract":"<div><h3>Objectives</h3><div>Obstructive sleep apnea, a multisystem disease, affects cochlear and vestibular functions due to chronic intermittent hypoxia and recurrent oxygen desaturation. During rapid eye movement sleep, the muscle tone of the upper airway decreases more, and the cardiovascular complication risk is higher than that during non-rapid eye movement sleep. We analyzed whether tinnitus was associated with obstructive sleep apnea and rapid eye movement-related obstructive sleep apnea in patients with similar hearing thresholds.</div></div><div><h3>Methods</h3><div>We evaluated patients with obstructive sleep apnea without (24 patients; group 1) and with (27 patients; group 2) tinnitus. All participants underwent pure-tone audiometry, tinnitus tests, polysomnography, and sleep questionnaires.</div></div><div><h3>Results</h3><div>The sleep efficiency (86.23% ± 10.38% vs. 81.27% ± 8.10%) and Epworth Sleepiness Scale (8.50 ± 4.31 vs. 5.15 ± 2.82) values were higher in group 2 versus group 1. In patients with rapid eye movement-related obstructive sleep apnea, the sleep efficiency value was higher in group 2 (90.55% ± 4.61%) versus group 1 (78.13% ± 11.70%). However, in patients with obstructive sleep apnea and rapid eye movement-related obstructive sleep apnea, medication use that might affect sleep was higher in group 2 (44.4% and 54.4%, respectively) versus group 1 (16.7% and 28.6%, respectively).</div></div><div><h3>Conclusions</h3><div>We evaluated the relationship between tinnitus and obstructive sleep apnea or rapid eye movement-related obstructive sleep apnea in groups with similar hearing thresholds for the first time. The presence of tinnitus in patients with obstructive sleep apnea with similar hearing thresholds was more closely related to subjective parameters than to objective parameters. Patients with tinnitus had poorer sleep quality than those without tinnitus.</div></div><div><h3>Level of evidence</h3><div>4.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101661"},"PeriodicalIF":1.7,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aberrant expression of Bcl2L12 as a potential biomarker for predicting recurrence in nasal polyp","authors":"Ping Zhang ,&nbsp;Fengjun Wang","doi":"10.1016/j.bjorl.2025.101670","DOIUrl":"10.1016/j.bjorl.2025.101670","url":null,"abstract":"<div><h3>Objective</h3><div>Bcl2L12 plays an important role in the inflammatory response, but its role in chronic rhinitis with nasal polyps (CRSwNP) is unknown. We aimed to evaluate the relationship of Bcl2L12 with eosinophilic inflammation in CRSwNP and its value in predicting postoperative recurrence.</div></div><div><h3>Methods</h3><div>We included 80 patients with CRSwNP treated with Functional Endoscopic Sinus Surgery (FESS), and 40 Health Control (HC) subjects. The expression levels of Bcl2L12 in tissues were examined by RT-PCR, Western Blotting (WB) and Immunohistochemistry (IHC). Correlation analysis was performed to explore the relationship between the expression level of Bcl2L12 and eosinophilic inflammation of the disease. Receiver Operating Characteristics (ROC) curves were used to assess the relationship between Bcl2L12 levels and postoperative recurrence.</div></div><div><h3>Results</h3><div>RT-PCR results suggested that tissue Bcl2L12 levels were higher in the relapse CRSwNP group than in the control and primary CRSwNP group. Correlation analysis showed that elevated Bcl2L12 levels were positively correlated with disease eosinophilic inflammation (p &lt; 0.05). In addition, tissue Bcl2L12 levels were higher by WB and IHC in the CRSwNP group than in the HC group (p &lt; 0.05), especially in the recurrent group, and were centrally expressed in the epithelial region of the mucosa. Further regression analysis showed that blood eosinophil count and percentage, tissue eosinophil count, and tissue Bcl2L12 levels were closely associated with disease recurrence after surgery. The ROC curve suggested that tissue Bcl2L12 had a higher value in predicting postoperative recurrence.</div></div><div><h3>Conclusion</h3><div>Our study shows that Bcl2L12 is increased in patients with CRSwNP, and is associated with eosinophilic inflammation. Tissue Bcl2L12 levels may be an objective marker to predict the risk of postoperative recurrence.</div></div><div><h3>Level of evidence</h3><div>Level 3.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101670"},"PeriodicalIF":1.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bilateral middle ear cholesteatoma in children: A single-center retrospective study","authors":"Xiaoxu Wang ,&nbsp;Lining Guo ,&nbsp;Enxia Tian ,&nbsp;Min Chen ,&nbsp;Wei Liu ,&nbsp;Xin Ni ,&nbsp;Jie Zhang","doi":"10.1016/j.bjorl.2025.101671","DOIUrl":"10.1016/j.bjorl.2025.101671","url":null,"abstract":"<div><h3>Objectives</h3><div>To analyze the clinical characteristics of bilateral cholesteatoma in children and summarize the clinical treatment pathway.</div></div><div><h3>Methods</h3><div>In this retrospective cohort study, we analyzed the records of children with bilateral middle ear cholesteatoma who underwent surgery in our department between 2016 and 2023. The clinical characteristics, treatment and prognosis were analyzed.</div></div><div><h3>Results</h3><div>The records of 12-children (24-ears, 4girls and 8-boys) were reviewed. The mean age was 6.5 ± 2.6-years. Four children had Congenital Cholesteatoma (CC) in one ear and Acquired Cholesteatoma (AC) in the other ear and 8-children had AC in both ears. The onset time and lesion degree of the bilateral ears are different. Nine children had maxillary developmental deformities (75%). One child underwent simultaneous binaural surgery, and 11 underwent staged surgery, with a median interval of 3-months between surgeries. The follow-up period was 4.28 ± 2.58-years. No postoperative complications. Six children (7-ears) underwent revision surgery due to recurrence. There was no significant difference in hearing results between pure-tone average of preoperative (41.23 ± 16.50 dB) and postoperative (37.79 ± 14.42 dB) (p = 0.291, <em>t</em> = 1.082). There was no significant difference in postoperative hearing between CC and AC (p = 0.355, <em>F</em> = 4.268). There were significant differences in postoperation hearing among different surgical methods (p = 0.006, H = 12.630). There were significant differences in postoperation hearing among different ossiculoplasty methods (p = 0.001, H = 17.590).</div></div><div><h3>Conclusions</h3><div>In our cohort, the average age at diagnosis of bilateral cholesteatoma in children was notably young, with a high proportion presenting concurrent cleft palate deformities. Staged surgical intervention prioritizing the more severely affected ear was adopted for bilateral cases. Furthermore, long-term follow-up is necessary due to the observed high recurrence rate in this population. A management flowchart has been proposed based on our therapeutic experience.</div></div><div><h3>Level of evidence</h3><div>Level 4.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101671"},"PeriodicalIF":1.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends and future directions in chronic rhinosinusitis with nasal polyps: A bibliometric analysis","authors":"Yeming Zhong ,&nbsp;Xuan Wei ,&nbsp;Caiyun Zou ,&nbsp;Bo Qian ,&nbsp;Hongbo Ji ,&nbsp;Jinhe Guo ,&nbsp;Zigang Che","doi":"10.1016/j.bjorl.2025.101672","DOIUrl":"10.1016/j.bjorl.2025.101672","url":null,"abstract":"<div><h3>Objectives</h3><div>Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) is a complex inflammatory condition that significantly impairs patients’ quality of life. Despite significant efforts to develop effective treatments, a comprehensive bibliometric analysis in this field is lacking. This study aims to identify key trends, influential publications, leading authors, and collaborative networks.</div></div><div><h3>Methods</h3><div>A bibliometric analysis was conducted on October 16, 2024, examining publications in the Web of Science Core Collection (WoSCC) database from 2004 to 2024. Data visualization and interpretation were performed using VOSviewer (version 1.6.20), CiteSpace (version 6.3.R1), and R 4.3.3.</div></div><div><h3>Results</h3><div>The analysis included 1,235 publications from 5,825 authors across 4,723 institutions in 222 countries. The United States led in publication volume, followed by China and Italy. Major institutional contributors included Capital Medical University and Sanofi. The International Forum of Allergy &amp; Rhinology published the most articles, while the Journal of Allergy and Clinical Immunology had the highest citation count. Key authors identified were Bachert Claus and Gevaert Philippe. Keyword analysis revealed “asthma”, “endoscopic sinus surgery”, and “inflammation” as dominant themes, with burst analysis indicating growing interest in “humanization”, “type 2 inflammation”, “risk”, “biologics”, and “safety”.</div></div><div><h3>Conclusion</h3><div>This bibliometric analysis underscores an increasing focus on biologic therapies, inflammation management, and endoscopic sinus surgery in CRSwNP research. Future investigations are likely to prioritize the long-term safety and cost-effectiveness of biologics, alongside advancements in precision medicine and surgical techniques.</div></div><div><h3>Level of evidence</h3><div>Level 1.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101672"},"PeriodicalIF":1.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adequate vs. complete vs. full house surgery: What are we really talking about?","authors":"Miguel Soares Tepedino ,&nbsp;Renato Roithmann ,&nbsp;Fabiana Cardoso Pereira Valera ,&nbsp;Fabrizio Ricci Romano ,&nbsp;Eduardo Macoto Kosugi ,&nbsp;Otávio Bejzman Piltcher ,&nbsp;Leonardo Lopes Balsalobre Filho ,&nbsp;Edwin Tamashiro ,&nbsp;Wilma Terezinha Anselmo-Lima","doi":"10.1016/j.bjorl.2025.101687","DOIUrl":"10.1016/j.bjorl.2025.101687","url":null,"abstract":"","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 4","pages":"Article 101687"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144620988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of prognostic prediction model for submandibular gland cancer based on the SEER database","authors":"Junkun He ,&nbsp;Feng Zhao ,&nbsp;Jiangmiao Li,&nbsp;Qiyun Li,&nbsp;Fangyu Wei,&nbsp;Jiping Su","doi":"10.1016/j.bjorl.2025.101654","DOIUrl":"10.1016/j.bjorl.2025.101654","url":null,"abstract":"<div><h3>Objective</h3><div>Accurately predicting the prognosis of Submandibular Gland Carcinoma (SGC) patients remains a challenging task. The purpose of this study was to develop a columnar graph prognostic prediction model for submandibular gland cancer based on the SEER database, using feature selection with lasso regression and modeling with Cox regression.</div></div><div><h3>Methods</h3><div>This study utilized data from the SEER database, focusing on 1362 cases of SGC. Various clinical and demographic factors, including age, tumor size, histology, and lymph node metastasis, were considered as potential prognostic factors. Feature selection was performed using lasso regression, and a Cox proportional hazards model was constructed, taking into account the complex interactions between variables and their impact on survival outcomes.</div></div><div><h3>Results</h3><div>The established prognostic prediction model demonstrated good accuracy and reliability. The model effectively identified several important prognostic factors, including age, tumor size, histology, and lymph node metastasis, which strongly influenced the prognosis of SGC. The model showed good discrimination and calibration with c-indexes of 0.802 (0.784‒0.821) in the training set and 0.756 (0.725‒0.787) in the validation set. The Decision Curve Analysis (DCA) curve reflected clinical utility.</div></div><div><h3>Conclusion</h3><div>This study suggests that the prognostic prediction model based on Cox regression is a valuable tool for predicting the prognosis of patients with SGC. This approach has the potential to improve patient outcomes by facilitating personalized treatment plans and identifying high-risk patients who may benefit from more aggressive interventions.</div></div><div><h3>Level of Evidence</h3><div>Level III.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101654"},"PeriodicalIF":1.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-cultural adaptation and validation of the theory of Planned Behavior-Hearing Help Seeking: A methodological study","authors":"Yuxuan He ,&nbsp;Menghui Deng ,&nbsp;Li Wei ,&nbsp;Jia Liu ,&nbsp;Xiaomei Chen ,&nbsp;Yanni Yang","doi":"10.1016/j.bjorl.2025.101660","DOIUrl":"10.1016/j.bjorl.2025.101660","url":null,"abstract":"<div><h3>Objective</h3><div>Seeking help for hearing loss is a complex psychological reaction. The purpose of this investigation was to cross-culturally adapt and validate the Theory of Planned Behavior-Hearing Help Seeking (TPB-HHS) to Chinese language and culture.</div></div><div><h3>Methods</h3><div>A total of 184 middle-aged and older Chinese adults completed the TPB-HHS. The study explored the factor structure and psychometric properties of the Chinese TPB-HHS, including model fit, convergent validity, discriminant validity, internal consistency and retest reliability.</div></div><div><h3>Results</h3><div>Four factors were identified, explaining 65% of the variance, with significant internal consistency and model fitting. In addition, they had satisfactory retest reliability after one week. In addition, the TPB-HHS scale showed substantial convergent and discriminant validity.</div></div><div><h3>Conclusion</h3><div>The Chinese TPB-HHS version has been shown to be a reliable and effective tool for measuring hearing test intention in initial community hearing screening. It enables healthcare providers to determine the level of intent to seek help in patients with hearing loss, laying the foundation for interventions to help these patients achieve optimal physical, psychological, and social outcomes.</div></div><div><h3>Level of evidence</h3><div>2.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101660"},"PeriodicalIF":1.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma","authors":"Xi Yang ,&nbsp;Chun Feng ,&nbsp;Donghui Jiang ,&nbsp;Xin Xu ,&nbsp;Yingying Zhang ,&nbsp;Jin Wang ,&nbsp;Xiaoguang He","doi":"10.1016/j.bjorl.2025.101655","DOIUrl":"10.1016/j.bjorl.2025.101655","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to construct a ceRNA network and identify potential diagnostic biomarkers for HSCC.</div></div><div><h3>Methods</h3><div>The R software was used to identify Differentially Expressed circular RNAs (DE-circRNAs) and mRNAs (DE-mRNAs) between HSCC and Normal Control (NC) specimens. Univariate Cox analysis and survival curves were used to identify biomarkers associated with the prognosis of HSCC. In addition, the diagnostic value of the biomarkers and their relevance to the tumor microenvironment and immunotherapy were evaluated. A ceRNA network based on mRNAs, circRNAs, and miRNAs was established. Finally, qRT-PCR was used to evaluate the expression of biomarkers in HSCC and para-cancerous tissue samples.</div></div><div><h3>Results</h3><div>A network containing 90 miRNAs, 47 circRNAs, and 111 mRNAs was established via bioinformatic analysis. Three genes (NRG1, CCNG2, and CHSY1) were identified as prognostic and diagnostic biomarkers for HSCC. The low-CCNG2-expression and low-CHSY1-expression groups had higher survival rates, whereas the low-NRG1-expression group had a lower survival rate. The AUC values of the three biomarkers &gt; 0.9, which indicated that the diagnostic value of all biomarkers was excellent. Moreover, CCNG2 and CHSY1 were associated with the severity of malignancy and the effectiveness of immunotherapy. Subsequently, a ceRNA network containing 3 mRNAs, 7 miRNAs, and 7 circRNAs was established. The results of qRT-PCR validated that the expression patterns of NRG1 and CHSY1 in clinical HSCC and para-cancerous samples were consistent with those observed in the GSE2379 dataset.</div></div><div><h3>Conclusion</h3><div>In this study, we identified three diagnostic biomarkers for HSCC (NRG1, CCNG2, and CHSY1) and established their corresponding ceRNA network via bioinformatic analysis, providing novel insights into the screening and treatment of HSCC.</div></div>","PeriodicalId":49099,"journal":{"name":"Brazilian Journal of Otorhinolaryngology","volume":"91 5","pages":"Article 101655"},"PeriodicalIF":1.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}