Plant Genome最新文献_第9页

Genomic approaches to enhance adaptive plasticity to cope with soil constraints amidst climate change in wheat. 通过基因组学方法提高小麦的适应可塑性，以应对气候变化中的土壤约束。

IF 4.2 2区生物学

Plant Genome Pub Date : 2024-03-01 Epub Date: 2023-06-02 DOI: 10.1002/tpg2.20358

Roopali Bhoite, Yong Han, Alamuru Krishna Chaitanya, Rajeev K Varshney, Darshan Lal Sharma

{"title":"Genomic approaches to enhance adaptive plasticity to cope with soil constraints amidst climate change in wheat.","authors":"Roopali Bhoite, Yong Han, Alamuru Krishna Chaitanya, Rajeev K Varshney, Darshan Lal Sharma","doi":"10.1002/tpg2.20358","DOIUrl":"10.1002/tpg2.20358","url":null,"abstract":"Climate change is varying the availability of resources, soil physicochemical properties, and rainfall events, which collectively determines soil physical and chemical properties. Soil constraints-acidity (pH < 6), salinity (pH ≤ 8.5), sodicity, and dispersion (pH > 8.5)-are major causes of wheat yield loss in arid and semiarid cropping systems. To cope with changing environments, plants employ adaptive strategies such as phenotypic plasticity, a key multifaceted trait, to promote shifts in phenotypes. Adaptive strategies for constrained soils are complex, determined by key functional traits and genotype × environment × management interactions. The understanding of the molecular basis of stress tolerance is particularly challenging for plasticity traits. Advances in sequencing and high-throughput genomics technologies have identified functional alleles in gene-rich regions, haplotypes, candidate genes, mechanisms, and in silico gene expression profiles at various growth developmental stages. Our review focuses on favorable alleles for enhanced gene expression, quantitative trait loci, and epigenetic regulation of plant responses to soil constraints, including heavy metal stress and nutrient limitations. A strategy is then described for quantitative traits in wheat by investigating significant alleles and functional characterization of variants, followed by gene validation using advanced genomic tools, and marker development for molecular breeding and genome editing. Moreover, the review highlights the progress of gene editing in wheat, multiplex gene editing, and novel alleles for smart control of gene expression. Application of these advanced genomic technologies to enhance plasticity traits along with soil management practices will be an effective tool to build yield, stability, and sustainability on constrained soils in the face of climate change.","PeriodicalId":49002,"journal":{"name":"Plant Genome","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9933970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic analysis of near-isogenic lines reveals key biomarkers on wheat chromosome 4B conferring drought tolerance. 对近等基因系的蛋白质组分析揭示了小麦 4B 染色体上赋予抗旱性的关键生物标记。

IF 4.2 2区生物学

Plant Genome Pub Date : 2024-03-01 Epub Date: 2023-05-18 DOI: 10.1002/tpg2.20343

Sina Nouraei, Md Sultan Mia, Hui Liu, Neil C Turner, Javed M Khan, Guijun Yan

{"title":"Proteomic analysis of near-isogenic lines reveals key biomarkers on wheat chromosome 4B conferring drought tolerance.","authors":"Sina Nouraei, Md Sultan Mia, Hui Liu, Neil C Turner, Javed M Khan, Guijun Yan","doi":"10.1002/tpg2.20343","DOIUrl":"10.1002/tpg2.20343","url":null,"abstract":"Drought is a major constraint for wheat production that is receiving increased attention due to global climate change. This study conducted isobaric tags for relative and absolute quantitation proteomic analysis on near-isogenic lines to shed light on the underlying mechanism of qDSI.4B.1 quantitative trait loci (QTL) on the short arm of chromosome 4B conferring drought tolerance in wheat. Comparing tolerant with susceptible isolines, 41 differentially expressed proteins were identified to be responsible for drought tolerance with a p-value of < 0.05 and fold change >1.3 or <0.7. These proteins were mainly enriched in hydrogen peroxide metabolic activity, reactive oxygen species metabolic activity, photosynthetic activity, intracellular protein transport, cellular macromolecule localization, and response to oxidative stress. Prediction of protein interactions and pathways analysis revealed the interaction between transcription, translation, protein export, photosynthesis, and carbohydrate metabolism as the most important pathways responsible for drought tolerance. The five proteins, including 30S ribosomal protein S15, SRP54 domain-containing protein, auxin-repressed protein, serine hydroxymethyltransferase, and an uncharacterized protein with encoding genes on 4BS, were suggested as candidate proteins responsible for drought tolerance in qDSI.4B.1 QTL. The gene coding SRP54 protein was also one of the differentially expressed genes in our previous transcriptomic study.","PeriodicalId":49002,"journal":{"name":"Plant Genome","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9534477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long noncoding RNA transcriptome analysis reveals novel lncRNAs in Morus alba 'Yu-711' response to drought stress. 长链非编码RNA转录组分析揭示了桑'Yu-711'对干旱胁迫的新lncRNAs。

IF 4.2 2区生物学

Plant Genome Pub Date : 2024-03-01 Epub Date: 2022-10-26 DOI: 10.1002/tpg2.20273

Michael Ackah, Xin Jin, Qiaonan Zhang, Frank Kwarteng Amoako, Lei Wang, Thomas Attaribo, Mengdi Zhao, Feng Yuan, Richard Ansah Herman, Changyu Qiu, Qiang Lin, Zhi Yin, Weiguo Zhao

{"title":"Long noncoding RNA transcriptome analysis reveals novel lncRNAs in Morus alba 'Yu-711' response to drought stress.","authors":"Michael Ackah, Xin Jin, Qiaonan Zhang, Frank Kwarteng Amoako, Lei Wang, Thomas Attaribo, Mengdi Zhao, Feng Yuan, Richard Ansah Herman, Changyu Qiu, Qiang Lin, Zhi Yin, Weiguo Zhao","doi":"10.1002/tpg2.20273","DOIUrl":"10.1002/tpg2.20273","url":null,"abstract":"Drought stress has been a key environmental factor affecting plant growth and development. The plant genome is capable of producing long noncoding RNAs (lncRNAs). To better understand white mulberry (Morus alba L.) drought response mechanism, we conducted a comparative transcriptome study comparing two treatments: drought-stressed (EG) and well-watered (CK) plants. A total of 674 differentially expressed lncRNAs (DElncRNAs) were identified. In addition, 782 differentially expressed messenger RNAs (DEmRNAs) were identified. We conducted Gene Ontology (GO) and KEGG enrichment analyses focusing on the differential lncRNAs cis-target genes. The target genes of the DElncRNAs were most significantly involved in the biosynthesis of secondary metabolites. Gene regulatory networks of the target genes involving DElncRNAs-mRNAs-DEmRNAs and DElncRNA-miRNA-DEmRNA were constructed. In the DElncRNAs-DEmRNAs network, 30 DEmRNAs involved in the biosynthesis of secondary metabolites are collocated with 46 DElncRNAs. The interaction between DElncRNAs and candidate genes was identified using LncTar. In summary, quantitative real-time polymerase chain reaction (qRT-PCR) validated nine candidate genes and seven target lncRNAs including those identified by LncTar. We predicted that the DElncRNAs-DEmRNAs might recruit microRNAs (miRNAs) to interact with gene regulatory networks under the drought stress response in mulberry. The findings will contribute to our understanding of the regulatory functions of lncRNAs under drought stress and will shed new light on the mulberry-drought stress interactions.","PeriodicalId":49002,"journal":{"name":"Plant Genome","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43042296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the CMS genetic regulation through comparative complete mitochondrial genome sequencing in Nicotiana tabacum. 通过比较全线粒体基因组测序对烟草CMS基因调控的研究。

IF 4.2 2区生物学

Plant Genome Pub Date : 2024-03-01 Epub Date: 2023-11-14 DOI: 10.1002/tpg2.20409

Dongli He, Yifan Li, Cheng Yuan, Xiaoxiong Pei, Rebecca N Damaris, Haiqin Yu, Bao Qian, Yong Liu, Bin Yi, Changjun Huang, Jianmin Zeng

{"title":"Characterization of the CMS genetic regulation through comparative complete mitochondrial genome sequencing in Nicotiana tabacum.","authors":"Dongli He, Yifan Li, Cheng Yuan, Xiaoxiong Pei, Rebecca N Damaris, Haiqin Yu, Bao Qian, Yong Liu, Bin Yi, Changjun Huang, Jianmin Zeng","doi":"10.1002/tpg2.20409","DOIUrl":"10.1002/tpg2.20409","url":null,"abstract":"Mitochondrial genomes (mitogenomes) of flowering plants vary greatly in structure and size, which can lead to frequent gene mutation, rearrangement, or recombination, then result in the cytoplasmic male sterile (CMS) mutants. In tobacco (Nicotiana tabacum), suaCMS lines are widely used in heterosis breeding; however, the related genetic regulations are not very clear. In this study, the cytological observation indicated that the pollen abortion of tobacco suaCMS(HD) occurred at the very early stage of the stamen primordia differentiation. In this study, the complete mitochondrial genomes of suaCMS(HD) and its maintainer HD were sequenced using the PacBio and Illumina Hiseq technology. The total length of the assembled mitogenomes of suaCMS(HD) and HD was 494,317 bp and 430,694 bp, respectively. Comparative analysis indicated that the expanded 64 K bases in suaCMS(HD) were mainly located in noncoding regions, and 23 and 21 big syntenic blocks (>5000 bp) were found in suaCMS(HD) and HD with a series of repeats. Electron transport chain-related genes were highly conserved in two mitogenomes, except five genes (ATP4, ATP6, COX2, CcmFC, and SDH3) with substantial substitutions. Three suaCMS(HD)-specific genes, orf261, orf291, and orf433, were screened. Sequence analysis and RT-PCR verification showed that they were unique to suaCMS(HD). Further gene location analysis and protein property prediction indicated that all the three genes were likely candidates for suaCMS(HD). This study provides new insight into understanding the suaCMS mechanism and is useful for improving tobacco breeding.","PeriodicalId":49002,"journal":{"name":"Plant Genome","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92157069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Durum wheat heat tolerance loci defined via a north-south gradient. 硬粒小麦耐热基因座由南北梯度确定。

IF 4.2 2区生物学

Plant Genome Pub Date : 2024-03-01 Epub Date: 2023-12-07 DOI: 10.1002/tpg2.20414

Amadou Tidiane Sall, Hafssa Kabbaj, Sidi Ould Ely Menoum, Madiama Cisse, Mulatu Geleta, Rodomiro Ortiz, Filippo M Bassi

{"title":"Durum wheat heat tolerance loci defined via a north-south gradient.","authors":"Amadou Tidiane Sall, Hafssa Kabbaj, Sidi Ould Ely Menoum, Madiama Cisse, Mulatu Geleta, Rodomiro Ortiz, Filippo M Bassi","doi":"10.1002/tpg2.20414","DOIUrl":"10.1002/tpg2.20414","url":null,"abstract":"The global production of durum wheat (Triticum durum Desf.) is hindered by a constant rise in the frequency of severe heat stress events. To identify heat-tolerant germplasm, three different germplasm panels (\"discovery,\" \"investigation,\" and \"validation\") were studied under a range of heat-stressed conditions. Grain yield (GY) and its components were recorded at each site and a heat stress susceptibility index was calculated, confirming that each 1°C temperature rise corresponds to a GY reduction in durum wheat of 4.6%-6.3%. A total of 2552 polymorphic single nucleotide polymorphisms (SNPs) defined the diversity of the first panel, while 5642 SNPs were polymorphic in the \"investigation panel.\" The use of genome-wide association studies revealed that 36 quantitative trait loci were associated with the target traits in the discovery panel, of which five were confirmed in a \"subset\" tested imposing heat stress by plastic tunnels, and in the investigation panel. A study of allelic combinations confirmed that Q.icd.Heat.003-1A, Q.icd.Heat.007-1B, and Q.icd.Heat.016-3B are additive in nature and the positive alleles at all three loci resulted in a 16% higher GY under heat stress. The underlying SNPs were converted into kompetitive allele specific PCR markers and tested on the validation panel, confirming that each explained up to 9% of the phenotypic variation for GY under heat stress. These markers can now be used for breeding to improve resilience to climate change and increase productivity in heat-stressed areas.","PeriodicalId":49002,"journal":{"name":"Plant Genome","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138499950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Climate change impacts on crop breeding: Targeting interacting biotic and abiotic stresses for wheat improvement. 气候变化对作物育种的影响：针对相互作用的生物和非生物胁迫进行小麦改良。

IF 4.2 2区生物学

Plant Genome Pub Date : 2024-03-01 Epub Date: 2023-07-06 DOI: 10.1002/tpg2.20365

Carlos A Robles-Zazueta, Leonardo A Crespo-Herrera, Francisco J Piñera-Chavez, Carolina Rivera-Amado, Gudbjorg I Aradottir

{"title":"Climate change impacts on crop breeding: Targeting interacting biotic and abiotic stresses for wheat improvement.","authors":"Carlos A Robles-Zazueta, Leonardo A Crespo-Herrera, Francisco J Piñera-Chavez, Carolina Rivera-Amado, Gudbjorg I Aradottir","doi":"10.1002/tpg2.20365","DOIUrl":"10.1002/tpg2.20365","url":null,"abstract":"Wheat (Triticum aestivum L.) as a staple crop is closely interwoven into the development of modern society. Its influence on culture and economic development is global. Recent instability in wheat markets has demonstrated its importance in guaranteeing food security across national borders. Climate change threatens food security as it interacts with a multitude of factors impacting wheat production. The challenge needs to be addressed with a multidisciplinary perspective delivered across research, private, and government sectors. Many experimental studies have identified the major biotic and abiotic stresses impacting wheat production, but fewer have addressed the combinations of stresses that occur simultaneously or sequentially during the wheat growth cycle. Here, we argue that biotic and abiotic stress interactions, and the genetics and genomics underlying them, have been insufficiently addressed by the crop science community. We propose this as a reason for the limited transfer of practical and feasible climate adaptation knowledge from research projects into routine farming practice. To address this gap, we propose that novel methodology integration can align large volumes of data available from crop breeding programs with increasingly cheaper omics tools to predict wheat performance under different climate change scenarios. Underlying this is our proposal that breeders design and deliver future wheat ideotypes based on new or enhanced understanding of the genetic and physiological processes that are triggered when wheat is subjected to combinations of stresses. By defining this to a trait and/or genetic level, new insights can be made for yield improvement under future climate conditions.","PeriodicalId":49002,"journal":{"name":"Plant Genome","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9755806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of terminal heat stress on osmolyte accumulation and gene expression during grain filling in bread wheat (Triticum aestivum L.). 终期热胁迫对面包小麦（Triticum aestivum L.）籽粒灌浆期渗透质积累和基因表达的影响

IF 4.2 2区生物学

Plant Genome Pub Date : 2024-03-01 Epub Date: 2023-02-07 DOI: 10.1002/tpg2.20307

Pooja Sihag, Upendra Kumar, Vijeta Sagwal, Prexha Kapoor, Yogita Singh, Sheetal Mehla, Priyanka Balyan, Reazul Rouf Mir, Rajeev K Varshney, Krishna Pal Singh, Om Parkash Dhankher

{"title":"Effect of terminal heat stress on osmolyte accumulation and gene expression during grain filling in bread wheat (Triticum aestivum L.).","authors":"Pooja Sihag, Upendra Kumar, Vijeta Sagwal, Prexha Kapoor, Yogita Singh, Sheetal Mehla, Priyanka Balyan, Reazul Rouf Mir, Rajeev K Varshney, Krishna Pal Singh, Om Parkash Dhankher","doi":"10.1002/tpg2.20307","DOIUrl":"10.1002/tpg2.20307","url":null,"abstract":"The grain-filling stage in Triticum aestivum (wheat) is highly vulnerable to increasing temperature as terminal heat stress diminishes grain quality and yield. To examine the mechanism of terminal heat tolerance, we performed the biochemical and gene expression analyses using two heat-tolerant (WH730 and WH1218) and two heat-sensitive (WH711 and WH157) wheat genotypes. We observed a significant increase in total soluble sugar (25%-47%), proline (7%-15%), and glycine betaine (GB) (22%-34%) contents in flag leaf, whereas a decrease in grain-filling duration, 1000-kernel weight (8%-25%), and grain yield per plant (11%-23%) was observed under the late-sown compared to the timely sown. The maximum content of osmolytes, including total soluble sugar, proline, and GB, was observed in heat-tolerant genotypes compared to heat-sensitive genotypes. The expression of 10 heat-responsive genes associated with heat shock proteins (sHsp-1, Hsp17, and HsfA4), flavonoid biosynthesis (F3'-1 and PAL), β-glucan synthesis (CslF6 and CslH), and xyloglucan metabolism (XTH1, XTH2, and XTH5) was studied in flag leaf exposed to different heat treatments (34, 36, 38, and 40°C) at 15 days after anthesis by quantitative real-time polymerase chain reaction. A significant increase in the relative fold expression of these genes with increasing temperature indicated their involvement in providing heat-stress tolerance. The high differential expression of most of the genes in heat-tolerant genotype \"WH730\" followed by \"WH1218\" indicates the high adaptability of these genotypes to heat stress compared to heat-sensitive wheat genotypes. Based on the previous results, \"WH730\" performed better in terms of maximum osmolyte accumulation, grain yield, and gene expression under heat stress.","PeriodicalId":49002,"journal":{"name":"Plant Genome","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9222527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RADseq-based population genomic analysis and environmental adaptation of rare and endangered recretohalophyte Reaumuria trigyna. 基于 RADseq 的种群基因组分析和稀有濒危三尖杉（Reaumuria trigyna）的环境适应性。

IF 4.2 2区生物学

Plant Genome Pub Date : 2024-03-01 Epub Date: 2023-02-05 DOI: 10.1002/tpg2.20303

Zhenhua Dang, Jiabin Li, Yanan Liu, Miaomiao Song, Peter J Lockhart, Yunyun Tian, Miaomiao Niu, Qinglang Wang

{"title":"RADseq-based population genomic analysis and environmental adaptation of rare and endangered recretohalophyte Reaumuria trigyna.","authors":"Zhenhua Dang, Jiabin Li, Yanan Liu, Miaomiao Song, Peter J Lockhart, Yunyun Tian, Miaomiao Niu, Qinglang Wang","doi":"10.1002/tpg2.20303","DOIUrl":"10.1002/tpg2.20303","url":null,"abstract":"Genetic diversity reflects the survival potential, history, and population dynamics of an organism. It underlies the adaptive potential of populations and their response to environmental change. Reaumuria trigyna is an endemic species in the Eastern Alxa and West Ordos desert regions in China. The species has been considered a good candidate to explore the unique survival strategies of plants that inhabit this area. In this study, we performed population genomic analyses based on restriction-site associated DNA sequencing to understand the genetic diversity, population genetic structure, and differentiation of the species. Analyses of 92,719 high-quality single-nucleotide polymorphisms (SNPs) indicated that overall genetic diversity of R. trigyna was low (HO = 0.249 and HE = 0.208). No significant genetic differentiation was observed among the investigated populations. However, a subtle population genetic structure was detected. We suggest that this might be explained by adaptive diversification reinforced by the geographical isolation of populations. Overall, 3513 outlier SNPs were located in 243 gene-coding sequences in the R. trigyna transcriptome. Potential sites under diversifying selection occurred in genes (e.g., AP2/EREBP, E3 ubiquitin-protein ligase, FLS, and 4CL) related to phytohormone regulation and synthesis of secondary metabolites which have roles in adaptation of species. Our genetic analyses provide scientific criteria for evaluating the evolutionary capacity of R. trigyna and the discovery of unique adaptions. Our findings extend knowledge of refugia, environmental adaption, and evolution of germplasm resources that survive in the Ordos area.","PeriodicalId":49002,"journal":{"name":"Plant Genome","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10708170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated multi-omics analysis reveals drought stress response mechanism in chickpea (Cicer arietinum L.). 多组学综合分析揭示鹰嘴豆（Cicer arietinum L. ）的干旱胁迫响应机制

IF 4.2 2区生物学

Plant Genome Pub Date : 2024-03-01 Epub Date: 2023-05-10 DOI: 10.1002/tpg2.20337

Himabindu Kudapa, Arindam Ghatak, Rutwik Barmukh, Palak Chaturvedi, Aamir Khan, Sandip Kale, Lena Fragner, Annapurna Chitikineni, Wolfram Weckwerth, Rajeev K Varshney

{"title":"Integrated multi-omics analysis reveals drought stress response mechanism in chickpea (Cicer arietinum L.).","authors":"Himabindu Kudapa, Arindam Ghatak, Rutwik Barmukh, Palak Chaturvedi, Aamir Khan, Sandip Kale, Lena Fragner, Annapurna Chitikineni, Wolfram Weckwerth, Rajeev K Varshney","doi":"10.1002/tpg2.20337","DOIUrl":"10.1002/tpg2.20337","url":null,"abstract":"Drought is one of the major constraints limiting chickpea productivity. To unravel complex mechanisms regulating drought response in chickpea, we generated transcriptomics, proteomics, and metabolomics datasets from root tissues of four contrasting drought-responsive chickpea genotypes: ICC 4958, JG 11, and JG 11+ (drought-tolerant), and ICC 1882 (drought-sensitive) under control and drought stress conditions. Integration of transcriptomics and proteomics data identified enriched hub proteins encoding isoflavone 4'-O-methyltransferase, UDP-d-glucose/UDP-d-galactose 4-epimerase, and delta-1-pyrroline-5-carboxylate synthetase. These proteins highlighted the involvement of pathways such as antibiotic biosynthesis, galactose metabolism, and isoflavonoid biosynthesis in activating drought stress response mechanisms. Subsequently, the integration of metabolomics data identified six metabolites (fructose, galactose, glucose, myoinositol, galactinol, and raffinose) that showed a significant correlation with galactose metabolism. Integration of root-omics data also revealed some key candidate genes underlying the drought-responsive \"QTL-hotspot\" region. These results provided key insights into complex molecular mechanisms underlying drought stress response in chickpea.","PeriodicalId":49002,"journal":{"name":"Plant Genome","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9449304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding role of roots in plant response to drought: Way forward to climate-resilient crops. 了解根系在植物对干旱反应中的作用：气候适应性作物的发展方向。

IF 4.2 2区生物学

Plant Genome Pub Date : 2024-03-01 Epub Date: 2023-10-18 DOI: 10.1002/tpg2.20395

Anmol Kalra, Shailendra Goel, Ani A Elias

引用次数: 0