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Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision. Z/EG是一种双报告小鼠系，在cre介导的切除中表达增强的绿色荧光蛋白。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-11-01
A Novak, C Guo, W Yang, A Nagy, C G Lobe
{"title":"Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision.","authors":"A Novak,&nbsp;C Guo,&nbsp;W Yang,&nbsp;A Nagy,&nbsp;C G Lobe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Cre/loxP system has become an important tool in designing postintegrational switch mechanisms for transgenes in mice. The power and spectrum of application of this system depends on transgenic mouse lines that provide Cre recombinase activity with a defined cell type-, tissue-, or developmental stage-specificity. We have developed a novel mouse line that acts as a Cre reporter. The mice, designated Z/EG (lacZ/EGFP), express lacZ throughout embryonic development and adult stages. Cre excision, however, removes the lacZ gene, which activates expression of the second reporter, enhanced green fluorescent protein. We have found that the double-reporter Z/EG line is able to indicate the occurrence of Cre excision from early embryonic to adult lineages. The advantage of the Z/EG line is that Cre-mediated excision can be monitored in live samples and that live cells with Cre-mediated excision can be isolated using a single-step FACS. It will be a valuable reagent for the increasing number of investigators taking advantage of the powerful tools provided by the Cre/loxP site-specific recombinase system.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mouse embryonic stem (ES) cell lines established from neuronal cell-derived cloned blastocysts. 从神经细胞衍生克隆囊胚建立小鼠胚胎干细胞系。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-11-01
E Kawase, Y Yamazaki, T Yagi, R Yanagimachi, R A Pedersen
{"title":"Mouse embryonic stem (ES) cell lines established from neuronal cell-derived cloned blastocysts.","authors":"E Kawase,&nbsp;Y Yamazaki,&nbsp;T Yagi,&nbsp;R Yanagimachi,&nbsp;R A Pedersen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have established mouse embryonic stem (ES) cell lines from blastocysts derived by transfer of nuclei of fetal neuronal cells. These neuronal cell-derived embryonic cell lines had properties that characterize them as ES cells, including typical cell markers and alkaline phosphatase activity. Moreover, the cells had a normal karyotype and were pluripotent, as they were capable of differentiating into all three germ layers. Although they were derived from neuronal donor nuclei, the cells no longer expressed neuronal markers; however, they were capable of differentiating into cells with neuronal characteristics. These results suggest that the clone-derived cells have fully acquired an ES cell character. Thus, ES cells can be derived from embryos resulting from nuclear transfer, which results in reprogramming of the genetic information and acquisition of pluripotency. ES cells established from somatic cell-derived blastocysts could be useful not only as research tools for studying reprogramming but also as models for cell-based transplantation therapy.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21930488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Function of Rx, but not Pax6, is essential for the formation of retinal progenitor cells in mice. 小鼠视网膜祖细胞的形成离不开 Rx 而非 Pax6 的功能。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-11-01
L Zhang, P H Mathers, M Jamrich
{"title":"Function of Rx, but not Pax6, is essential for the formation of retinal progenitor cells in mice.","authors":"L Zhang, P H Mathers, M Jamrich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Rx plays a critical role in eye formation. Targeted elimination of Rx results in embryos that do not develop eyes. In this study, we have investigated the expression of Otx2, Six3, and Pax6 in Rx deficient embryos. We find that these genes show normal activation in the anterior neural plate in Rx-/- embryos, but they are not upregulated in the area of the neural plate that would form the primordium of the optic vesicle. In contrast, in homozygous Small eye embryos that lack Pax6 function, Rx shows normal activation in the anterior neural plate and normal upregulation in the optic vesicle/retinal progenitor cells. This suggests that neither Rx expression nor the formation of retinal progenitor cells is dependent on a functional copy of the Pax6 gene, but that Pax6 expression and the formation of the progenitor cells of the optic cup is dependent on a functional copy of the Rx gene.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cre recombinase expression in cerebellar Purkinje cells. Cre重组酶在小脑浦肯野细胞中的表达。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-11-01
J J Barski, K Dethleffsen, M Meyer
{"title":"Cre recombinase expression in cerebellar Purkinje cells.","authors":"J J Barski,&nbsp;K Dethleffsen,&nbsp;M Meyer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The cerebellar cortex and its sole output, the Purkinje cell, have been implicated in motor coordination, learning and cognitive functions. Therefore, the ability to generate Purkinje cell-specific mutations in physiologically relevant genes is of particular neurobiological interest. A suitable approach is the Cre/loxP strategy that allows temporally and spatially controlled gene inactivation. Here, we present the characterization of transgenic mouse strains expressing Cre recombinase controlled by the L7/pcp-2 gene. Endogenous L7/pcp-2 protein is expressed exclusively in Purkinje cells and retinal bipolar neurones. Recombination was detected by beta-galactosidase histochemistry in tissues from crosses of the L7/pcp-2:Cre transgenic lines with two different indicator strains, GtROSA26 and ACZL. Purkinje cells in all folia of the cerebellum displayed intense beta-galactosidase staining, whereas only few blue cells were observed in the retina and other parts of the CNS. Thus, these transgenic lines are potentially of great importance for genetic manipulations in cerebellar Purkinje cells.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21928717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evolution and development at the National Science Foundation, U.S.A. 美国国家科学基金会的进化与发展
IF 1.5 4区 生物学
Genesis Pub Date : 2000-10-01
J Plesset, S Scheiner, S Singer
{"title":"Evolution and development at the National Science Foundation, U.S.A.","authors":"J Plesset,&nbsp;S Scheiner,&nbsp;S Singer","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":48923,"journal":{"name":"Genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21892709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic analysis of the exed region in mouse. 小鼠外显区遗传分析。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-08-01 DOI: 10.1002/1526-968x(200008)27:4<174::aid-gene60>3.3.co;2-w
S K Kendall, S J Strong, R T Litman, G W Litman, T Magnuson
{"title":"Genetic analysis of the exed region in mouse.","authors":"S K Kendall,&nbsp;S J Strong,&nbsp;R T Litman,&nbsp;G W Litman,&nbsp;T Magnuson","doi":"10.1002/1526-968x(200008)27:4<174::aid-gene60>3.3.co;2-w","DOIUrl":"https://doi.org/10.1002/1526-968x(200008)27:4<174::aid-gene60>3.3.co;2-w","url":null,"abstract":"<p><p>The extraembryonic ectoderm development (exed) mutant phenotype was described in mice homozygous for the c(6H) deletion, a radiation-induced deletion in the tyrosinase region of mouse Chromosome 7. These mutants fail to gastrulate and die around embryonic day 8.0. Several genes including, for example, embryonic ectoderm development (eed), are deleted in the c(6H) mutants; however, the portion of the chromosome responsible for the more severe exed phenotype is localized to a 20-kb region called the \"exed-critical region.\" To understand the genetics behind the exed phenotype, we analyzed this region in two ways. First, to determine whether the 20-kb exed-critical region alone causes the mutant phenotype, we removed it from a wild-type chromosome. The resulting mice homozygous for this deletion were viable and fertile, indicating that the 20-kb exed-critical region by itself is not sufficient to cause the phenotype when deleted. We then sequenced the 20-kb exed-critical region and no expressed exons were found. Several short matches to GenBank Expressed Sequence Tag (EST) databases were identified; however, none of these ESTs mapped to the region. Taken together, these results indicate that the exed phenotype may either be a position effect on a distal gene caused by the c(6H) breakpoint or the result of composite effects of nullizygosity of multiple genes in the deletion homozygotes.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21827170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Absence of SOX3 in the developing marsupial gonad is not consistent with a conserved role in mammalian sex determination. 在发育中的有袋动物性腺中缺少SOX3与哺乳动物性别决定中的保守作用不一致。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-08-01
A J Pask, J L Harry, M B Renfree, J A Marshall Graves
{"title":"Absence of SOX3 in the developing marsupial gonad is not consistent with a conserved role in mammalian sex determination.","authors":"A J Pask,&nbsp;J L Harry,&nbsp;M B Renfree,&nbsp;J A Marshall Graves","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Expression of Sox3 has been detected in the testes of humans and of developing and adult mice at the same time as Sox9 and Sry. The co-expression of these three related Sox genes in the mouse indifferent gonadal ridge led to the hypothesis that these three genes, encoding transcription factors with similar DNA target binding sites, may interact with each other in initiating testis differentiation. The location of SOX3 on the marsupial Dunnart X chromosome also makes it a candidate for the marsupial X-linked gene responsible for the SRY- and hormone-independent initiation of scrotum or mammary gland development. Here we show that although marsupial SOX3 is highly conserved at the genetic level and appears to have a conserved role in CNS development, its expression during sexual differentiation differs from that of mice and humans. SOX3 expression is absent from the developing marsupial genital ridge and from the scrotal and mammary primordia during the critical time of differentiation and throughout the time that SRY is expressed. The absence of expression in the developing gonad strongly suggests that SOX3 does not have a conserved role in mammalian sexual determination or differentiation.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21827167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A subtractive gene expression screen suggests a role for vanin-1 in testis development in mice. 减法基因表达筛选表明，在小鼠睾丸发育中，vanin-1的作用。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-07-01
J Bowles, M Bullejos, P Koopman
{"title":"A subtractive gene expression screen suggests a role for vanin-1 in testis development in mice.","authors":"J Bowles,&nbsp;M Bullejos,&nbsp;P Koopman","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The molecular pathways leading from indifferent mammalian gonad to either testis or ovary are not well understood. A number of genes, including the Y-linked sex determining gene SRY, have been shown to play roles in sex determination or differentiation, but there are clearly many missing elements to be found. We used suppression-subtractive hybridization to construct normalized cDNA libraries enriched for male-specific or female-specific transcripts in mouse fetal gonads. We describe the strategy used to efficiently screen these libraries for candidate sex-determination and gonadogenesis genes. One gene arising from these screens is vanin-1, which encodes a protein implicated in the induction of cell migration into the thymus. We find that vanin-1 is expressed male-specifically in Sertoli cells of the developing testis and may be involved in inducing cell migration from the adjacent mesonephros, a process known to be critical for testis development. This screening approach is likely to be applicable to the isolation and study of genes involved in a variety of developmental systems.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21788331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rescue of embryonic lethality in hepatocyte growth factor/scatter factor knockout mice. 肝细胞生长因子/分散因子敲除小鼠胚胎致死性的恢复。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-07-01
Y Uehara, C Mori, T Noda, K Shiota, N Kitamura
{"title":"Rescue of embryonic lethality in hepatocyte growth factor/scatter factor knockout mice.","authors":"Y Uehara,&nbsp;C Mori,&nbsp;T Noda,&nbsp;K Shiota,&nbsp;N Kitamura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>While the targeted disruption of a gene is a powerful tool for investigating the physiological functions of that gene, disruption of a gene essential for embryogenesis leads to embryonic death. Rescue of the defect(s) causing embryonic death should promote survival, thus permitting further evaluation of the roles that the gene plays later in the developmental process. Disruption of the gene for mouse hepatocyte growth factor/scatter factor (HGF/SF) leads to middle-stage embryonic lethality because of a defect in placental development. Here we report that a single injection of HGF/SF at embryonic day 9.5 (E9.5) into the amniotic cavity of HGF/SF(-/-) embryos rescued the placental defect and resulted in the survival of the embryos until term. Histological analysis suggested that HGF/SF is also required at the late stage of development for tissue organogenesis. Thus, injection of a secreted factor can be a useful method to rescue the defects causing embryonic lethality.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21788408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Micro-capillary tube in situ hybridisation: a novel method for processing small individual samples. 微毛细管原位杂交:处理小个体样品的新方法。
IF 1.5 4区 生物学
Genesis Pub Date : 2000-06-01
A A Avilion, D M Bell, R Lovell-Badge
{"title":"Micro-capillary tube in situ hybridisation: a novel method for processing small individual samples.","authors":"A A Avilion,&nbsp;D M Bell,&nbsp;R Lovell-Badge","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have developed a strategy to individually analyse large numbers of small tissue samples by RNA in situ hybridisation. Samples of approximately 0.4 mm x 0.5 mm are processed in rectangular capillary tubes fitted with nylon mesh and glass beads using standard protocols. Eighteen samples can be assayed simultaneously without loss, and background is low. Specifically, mouse Sox2 RNA expression is examined in the chorion of extraembryonic tissue of 7.5 days post-coitum embryos. This technique works equally well for double RNA labelling and could potentially be used for antibody staining of proteins.</p>","PeriodicalId":48923,"journal":{"name":"Genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21733074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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