Botanical Studies最新文献

{"title":"The complete chloroplast genome and phylogentic results support the species position of Swertia banzragczii and Swertia marginata (Gentianaceae) in Mongolia","authors":"Dashzeveg Oyuntsetseg, Nudkhuu Nyamgerel, S. Baasanmunkh, Batlai Oyuntsetseg, M. Urgamal, Jung Won Yoon, G. Bayarmaa, Hyeok Jae Choi","doi":"10.1186/s40529-024-00417-z","DOIUrl":"https://doi.org/10.1186/s40529-024-00417-z","url":null,"abstract":"","PeriodicalId":48844,"journal":{"name":"Botanical Studies","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140663890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cypripedium subtropicum embryo development and cytokinin requirements for asymbiotic germination.","authors":"Holger Perner,&nbsp;Rong Zhou,&nbsp;Wenqing Perner,&nbsp;Hong Jiang,&nbsp;Yung-I Lee","doi":"10.1186/s40529-022-00359-4","DOIUrl":"https://doi.org/10.1186/s40529-022-00359-4","url":null,"abstract":"<p><strong>Background: </strong>Cypripedium subtropicum is a unique, endangered lady's slipper orchid with evergreen leaves on non-dormant shoots that is native to southwestern China. This study documents the major developmental events in C. subtropicum seed development from fertilization to seed maturity, determines the optimum period for seed collection, and examines the cytokinin requirements for asymbiotic germination and protocorm survival.</p><p><strong>Results: </strong>Structural studies revealed that embryo development proceeded after successful fertilization at 60 days after pollination (DAP). At 105 DAP, a globular embryo with the shrinking inner seed coat was observed, and seeds collected at this time point exhibited optimal germination. After 120 DAP, most seeds had a mature embryo within the capsule, and within the cells of the embryo proper, numerous proteins/lipid bodies were present as the main storage products. In addition, the inner seed coat had compressed into a thin layer that tightly enclosed the embryo, while the outer seed coat had progressively elongated, resulting in a hair-like appearance of the mature seed. Histochemical staining using Nile red and toluidine blue O (TBO) indicated that the lignified inner and outer seed coats may lead to coat-imposed dormancy. Seeds collected at this stage germinated poorly. Analyses of cytokinin preferences and optimal concentrations for germination and protocorm survival showed that both 6-(γ,γ-dimethylallylamino) purine (2iP) and 6-benzylaminopurine (BA) enhanced germination compared with the control, although higher concentrations of BA (4 and 8 μM) suppressed germination. The protocorm survival rate improved with increasing 2iP concentration.</p><p><strong>Conclusions: </strong>This study provides a reproducible procedure for culturing immature seeds of C. subtropicum based on a defined time schedule of seed development. In addition, the cytokinin 2iP was shown to improve germination and protocorm survival. This study provides a scientific basis for seedling establishment through asymbiotic seed culture for further reintroduction efforts.</p>","PeriodicalId":48844,"journal":{"name":"Botanical Studies","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40386687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiology and metabonomics reveal differences in drought resistance among soybean varieties.","authors":"Xiyue Wang,&nbsp;Yongping Li,&nbsp;Xiaojing Wang,&nbsp;Xiaomei Li,&nbsp;Shoukun Dong","doi":"10.1186/s40529-022-00339-8","DOIUrl":"https://doi.org/10.1186/s40529-022-00339-8","url":null,"abstract":"<p><strong>Background: </strong>The soybean is an important food crop worldwide. Drought during the first pod stage significantly affects soybean yield, and understanding the metabolomic and physiological changes in soybeans under drought stress is crucial. This study identified the differential metabolites in initial pod stage soybean leaves under polyethylene glycol-simulated drought stress, using ultra performance liquid chromatography and tandem mass spectrometry, and the physiological indexes related to drought resistance.</p><p><strong>Results: </strong>Physiologically, drought resistance also generates enzyme and antioxidant activity; levels of superoxide dismutase, peroxidase, and catalase first increased and subsequently decreased, while those of soluble sugar, soluble protein, malondialdehyde, and proline content increased in both varieties. The contents of CAT, proline and soluble sugar in Heinong 44 (HN44) were higher than those in Heinong 65 (HN65), and the contents of MDA were lower than those in HN65. In metabolomics, the OPLS-DA model was used to screen different metabolites. KEGG analysis showed that the two varieties resisted drought through different ways. Amino acid metabolism and lipid metabolism play a key role in drought resistance of the two varieties, respectively. TCA cycle was one of the core pathways of drought resistance in two varieties. Changes in the content of L-Asparagine and citric acid may be one of the reasons for the difference in drought resistance between the two varieties.</p><p><strong>Conclusions: </strong>We think that the reasons of drought resistance among soybean varieties are as follows: the main metabolic pathways are different under drought stress; the contents of metabolites in these metabolic pathways are different; some physiological indexes are different, such as MDA, CAT, proline content and so on. Our study enhances the understanding of the metabolomic soybean drought stress response and provides a reference for soybean drought resistance breeding.</p>","PeriodicalId":48844,"journal":{"name":"Botanical Studies","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40325571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asymmetric sharing of pollinator fig wasps between two sympatric dioecious fig trees: a reflection of supply and demand or differences in the size of their figs?","authors":"Hui Yu,&nbsp;Zhiwei Zhang,&nbsp;Lu Liu,&nbsp;Yufen Cheng,&nbsp;Xiaoxia Deng,&nbsp;Simon T Segar,&nbsp;Stephen G Compton","doi":"10.1186/s40529-022-00338-9","DOIUrl":"https://doi.org/10.1186/s40529-022-00338-9","url":null,"abstract":"<p><strong>Background: </strong>Host specificity among pollinator fig wasps (Agaonidae) depends on host plant specific volatile cues, but fig wasps must also pass through a narrow physical barrier (the ostiole) if they are to pollinate and oviposit. Across South East Asia the dioecious shrub Ficus hirta is associated with at least ten pollinator species allied to Valisia javana. Ficus triloba has a single recorded pollinator, Valisia esquirolianae. Receptive figs of F. hirta are usually much smaller than those of F. triloba, but at a mainland site where F. hirta has atypically large figs we identified both V. esquirolianae and V. javana from both Ficus species using COI and ITS2 sequencing. To investigate whether this host overlap was exceptional we reared fig wasps from the two trees elsewhere and recorded features that may facilitate host transfer between them, including attractant volatiles, reproductive phenology and the sizes of their figs and fig wasps.</p><p><strong>Results: </strong>The two Ficus species were found to support both Valisia species at several of the sites, suggesting that the differences we detected in volatile profiles, ostiole sizes and pollinator head sizes are not strict barriers to host sharing. Valisia javana colonised F. triloba more frequently than V. esquirolianae colonised F. hirta.</p><p><strong>Conclusions: </strong>This asymmetric sharing of pollinators may reflect the relative abundance of the two species of fig wasps and differences in host reproductive phenology. Asynchronous flowering of individual F. hirta may favor local retention of pollinators, in contrast to the tree-wide synchrony of F. triloba figs, which can generate local shortages of V. esquirolianae. If the pollinator sharing by male figs of F. triloba and F. hirta also occurs in female figs then this could result in gene flow between them.</p>","PeriodicalId":48844,"journal":{"name":"Botanical Studies","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8941105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40313963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Develop an efficient inoculation technique for Fusarium solani isolate \"TJP-2178-10\" pathogeny assessment in Phalaenopsis orchids.","authors":"Shu-Yun Chen,&nbsp;Yan-Jeng Wu,&nbsp;Ting-Fang Hsieh,&nbsp;Jiunn-Feng Su,&nbsp;Wei-Chiang Shen,&nbsp;Yung-Hsiang Lai,&nbsp;Pen-Chih Lai,&nbsp;Wen-Huei Chen,&nbsp;Hong-Hwa Chen","doi":"10.1186/s40529-021-00310-z","DOIUrl":"https://doi.org/10.1186/s40529-021-00310-z","url":null,"abstract":"<p><strong>Background: </strong>Phalaenopsis is one of the important ornamental plants worldwide. It plays the most significant role in flower exportation in Taiwan. However, the yellow leaf disease caused by Fusarium spp. has reduced the orchid flower yield 10-50 % yearly. Varieties resistant to yellow leaf disease associated with Fusarium is urgently needed for orchid growers and breeders, and is the ultimate solution for the long-term goal. To achieve this, phenotyping is the first step and the most necessary information for further studies, such as resistance gene identification, quantitative trait loci identification, and genome-wide association study.</p><p><strong>Results: </strong>The inoculation of Fusarium was performed in either abbreviated stem or detached leaf, and the pros and cons were compared. The former is the general method of phenotyping for estimating the tolerance to yellow leaf disease of Phalaenopsis, but it is time-consuming and spacy, and thus not suitable for the assessment of large numbers of samples. In contrast, the latter not only showed a similar trend of disease severity with time reduced to only one fourth of the former one but also less space needed.</p><p><strong>Conclusions: </strong>This solution allows a better phenotyping approach for the fast detection of yellow leaf disease associated with Fusarium in a large number of Phalaenopsis samples.</p>","PeriodicalId":48844,"journal":{"name":"Botanical Studies","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40529-021-00310-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25536036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do halophytes and glycophytes differ in their interactions with arbuscular mycorrhizal fungi under salt stress? A meta-analysis.","authors":"Jing Pan, Fei Peng, Anna Tedeschi, Xian Xue, Tao Wang, Jie Liao, Wenjuan Zhang, Cuihua Huang","doi":"10.1186/s40529-020-00290-6","DOIUrl":"10.1186/s40529-020-00290-6","url":null,"abstract":"<p><strong>Background: </strong>Halophytes are better than glycophytes at employing mechanisms to avoid salt injury, but both types of plants can undergo damage due to high soil salinity. Arbuscular mycorrhizal fungi (AMF) can mitigate the damage from salt stress in both halophytes and glycophytes by enhancing salt tolerance and improving energy efficiency. However, variations in mycorrhizal symbiotic efficiency between halophytes and glycophytes were still poorly understood. Therefore, we evaluated the magnitude of AMF effects on plant growth and determined the mechanisms that regulate the growth response of halophytes and glycophytes by performing a meta-analysis of 916 studies (from 182 publications).</p><p><strong>Results: </strong>Arbuscular mycorrhizal fungi significantly enhance biomass accumulation, osmolytes synthesis (soluble sugar and soluble protein), nutrients acquisition (nitrogen, phosphorus, and potassium ion), antioxidant enzyme activities (superoxide dismutase and catalase), and photosynthetic capacity (chlorophyll and carotenoid contents, photosynthetic rate, stomatal conductance, and transpiration rate). AMF also substantially decreased sodium ion acquisition and malondialdehyde levels in both halophytes and glycophytes under salt stress conditions. Mycorrhizal halophytes deploy inorganic ions (potassium and calcium ions) and limited organic osmolytes (proline and soluble sugar) to achieve energy-efficient osmotic adjustment and further promote biomass accumulation. Mycorrhizal glycophytes depend on the combined actions of soluble sugar accumulation, nutrients acquisition, sodium ion exclusion, superoxide dismutase elevation, and chlorophyll synthesis to achieve biomass accumulation.</p><p><strong>Conclusions: </strong>Arbuscular mycorrhizal fungi inoculation is complementary to plant function under salt stress conditions, not only facilitating energy acquisition but also redistributing energy from stress defence to growth. Glycophytes are more dependent on AMF symbiosis than halophytes under salt stress conditions.</p>","PeriodicalId":48844,"journal":{"name":"Botanical Studies","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2020-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7167393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37848828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Further evidence of Ceratobasidium D.P. Rogers (Basidiomycota) serving as the ubiquitous fungal associate of Platanthera leucophaea (Orchidaceae) in the North American tallgrass prairie.","authors":"Hana L Thixton,&nbsp;Elizabeth J Esselman,&nbsp;Laura L Corey,&nbsp;Lawrence W Zettler","doi":"10.1186/s40529-020-00289-z","DOIUrl":"https://doi.org/10.1186/s40529-020-00289-z","url":null,"abstract":"<p><strong>Background: </strong>In the United States and Canada, ca. one-half of native orchid species are now threatened with extinction. A number of these species are restricted to tallgrass prairies of central North America, such as the Eastern Prairie Fringed Orchid, Platanthera leucophaea (Nutt.) Lindl.-a U.S. Federally threatened species.</p><p><strong>Results: </strong>We provide new records of fungi recovered from roots of P. leucophaea and five other orchid species inhabiting prairie sites in Illinois and neighboring states during a 10-year period (2008-2017). A total of 39 fungal endophytes were isolated from Cypripedium candidum (1), Platanthera lacera (1), P. leucophaea (32), P. peramoena (3), Spiranthes lacera (1), and S. magnicamporum (1), 31 (79%) of which were assignable to Ceratobasidium and the remainder to Tulasnella. These fungi were acquired from 16 different sites, 13 of which are new records including two new state records (Iowa, Wisconsin). Molecular analysis revealed that some Ceratobasidium strains were virtually identical despite being geographically isolated by > 300 km.</p><p><strong>Conclusions: </strong>This study, encompassing a decade of work, confirms that Platanthera leucophaea is a mycorrhizal specialist with heavy reliance on Ceratobasidium with the tallgrass prairie ecosystem of North America. Our isolation of Ceratobasidium from P. leucophaea spanning additional sites suggests that the association is widespread. Such information should provide conservationists and land managers with more confidence in developing protocols that facilitate the long-term conservation of this prairie orchid.</p>","PeriodicalId":48844,"journal":{"name":"Botanical Studies","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2020-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40529-020-00289-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37839515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xylaria insolita and X. subescharoidea: two newly described species collected from a termite nesting site in Hua-lien, Taiwan.","authors":"Huei-Mei Hsieh,&nbsp;Jyh-Ching Chou,&nbsp;Yu-Ming Ju","doi":"10.1186/s40529-020-00287-1","DOIUrl":"https://doi.org/10.1186/s40529-020-00287-1","url":null,"abstract":"<p><strong>Background: </strong>A number of Xylaria species are exclusively associated with nests of macrotermitine termites. A nesting site of Odontotermes formosanus in eastern Taiwan, which is the only macrotermitine termite known on the island, had been inundated during the raining season of 2010, and hundreds of Xylaria stromata emerged from it thereafter. A thorough examination of these stromata showed that they represent a mixture of different species.</p><p><strong>Results: </strong>Five Xylaria species were identified from the stromata collected from the nesting site, including two undescribed species, which are newly described as X. insolita and X. subescharoidea herein, and three known species X. brunneovinosa, X. escharoidea, and X. furcata.</p><p><strong>Conclusion: </strong>Totally, there are 28 Xylaria species growing on termite nests or ground in the world. Although O. formosanus is the only macrotermitine species known in Taiwan, the Xylaria diversity associated with its nests is fairly high; the species number has reached 12 with X. furcata, X. insolita, and X. subescharoidea added to the Taiwan mycobiota.</p>","PeriodicalId":48844,"journal":{"name":"Botanical Studies","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2020-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40529-020-00287-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37806430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phalaenopsis orchid miniaturization by overexpression of OsGA2ox6, a rice GA2-oxidase gene.","authors":"Kun-Ting Hsieh,&nbsp;Su-Hui Liu,&nbsp;I-Wen Wang,&nbsp;Liang-Jwu Chen","doi":"10.1186/s40529-020-00288-0","DOIUrl":"https://doi.org/10.1186/s40529-020-00288-0","url":null,"abstract":"<p><strong>Background: </strong>Phalaenopsis orchids are one of the most common potted orchids sold worldwide. Most Phalaenopsis cultivars have long inflorescences that cause shipping problems and increase handling costs. Miniaturization of Phalaenopsis orchids not only reduces overall production costs but also can expand the appeal of the orchids to a different group of consumers who prefer to keep flowers on desks or tabletops. Although some miniature Phalaenopsis plants can be obtained via hybridization or mutation, they are unpredictable and limited in variety. We therefore used the transgenic approach of overexpressing gibberellin 2-oxidase 6 (OsGA2ox6), a rice GA deactivation gene, to investigate its functional effect in miniaturizing Phalaenopsis and to create a stable miniaturization platform to facilitate a supply for the potential demands of the miniature flower market.</p><p><strong>Results: </strong>A commercial moth orchid, Phalaenopsis Sogo Yukidian 'SPM313', was transformed with the plasmid vector Ubi:OsGA2ox6 and successfully overexpressed the OsGA2ox6 gene in planta. The transgenic lines displayed darker-green, shorter, and wider leaves, thicker roots and much shorter flower spikes (10 cm vs 33 cm) than the nontransgenic line with a normal flower size and blooming ability and are therefore an ideal miniaturized form of Phalaenopsis orchids.</p><p><strong>Conclusions: </strong>We demonstrated that the ectopic expression of OsGA2ox6 can miniaturize Phalaenopsis Sogo Yukidian 'SPM313' while preserving its blooming ability, providing an alternative, useful method for miniaturizing Phalaenopsis species. This miniaturization by a transgenic approach can be further expanded by using GA2ox genes from different plant species or different gene variants, thereby expanding the technical platform for miniaturizing Phalaenopsis species to meet the potential demands of the miniature Phalaenopsis flower market.</p>","PeriodicalId":48844,"journal":{"name":"Botanical Studies","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2020-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40529-020-00288-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37806422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro propagation of bulblets and LC-MS/MS analysis of isosteroidal alkaloids in tissue culture derived materials of Chinese medicinal herb Fritillaria cirrhosa D. Don.","authors":"Hung-Chi Chang,&nbsp;Hui-Min Xie,&nbsp;Maw-Rong Lee,&nbsp;Chiu-Ying Lin,&nbsp;Mei-Kuen Yip,&nbsp;Dinesh Chandra Agrawal,&nbsp;Hsin-Sheng Tsay","doi":"10.1186/s40529-020-00286-2","DOIUrl":"https://doi.org/10.1186/s40529-020-00286-2","url":null,"abstract":"<p><strong>Background: </strong>Fritillaria cirrhosa, an important Chinese medicinal herb, is a Class-III protected and highly exploited species by pharmaceutical industry. Dwindling wild populations of species are unable to meet market demand. Therefore, this study was carried out to develop an in vitro propagation method for bulblet production. Also, the study aimed to carry out LC-MS/MS analysis of tissue culture-derived bulblets and callus for the presence of isosteroidal alkaloids (peimissine, verticine, and verticinone), and compare its quantities with commercially available crude drug samples.</p><p><strong>Results: </strong>In vitro seed germination (91%) of F. cirrhosa was achieved on Murashige and Skoog's basal medium (MSBM) supplemented with 6-benzylaminopurine (1 mg L^-1) and α-naphthalene-acetic-acid (0.4 mg L^-1). On transfer of germinated seeds from Petri-dishes to glass bottles containing hormone-free MSBM, 37.5% of seedlings developed bulblets after 3 months of incubation. Regeneration and multiplication of bulblets were achieved by culture of transverse sections of bulblets on 1/2 X MSBM. By repeated subcultures at an interval of 2 months, 3072 bulblets weighing 1270 g could be produced at the end of 5th subculture. LC-MS/MS analysis showed a significant presence of peimissine in in vitro bulblets while callus incubated in the dark showed presence of peimissine and verticine.</p><p><strong>Conclusion: </strong>The study reports an efficient in vitro propagation method of bulblets production of F. cirrhosa and presence of some isosteroidal alkaloids in tissue culture-derived bulblets and callus. The study could be of immense help in production of F. cirrhosa bulblets and callus under laboratory conditions round the year. Also, these results can be used further to investigate production of isosteroidal alkaloids in bioreactors at commercial scale using liquid and cell suspension cultures. Thus, we not only can reduce our dependence on collections from natural habitats, but also can help in in situ conservation of this important species.</p>","PeriodicalId":48844,"journal":{"name":"Botanical Studies","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2020-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40529-020-00286-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37772028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}