Phalaenopsis orchid miniaturization by overexpression of OsGA2ox6, a rice GA2-oxidase gene.

IF 4.1 3区 生物学 Q1 PLANT SCIENCES
Kun-Ting Hsieh, Su-Hui Liu, I-Wen Wang, Liang-Jwu Chen
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引用次数: 8

Abstract

Background: Phalaenopsis orchids are one of the most common potted orchids sold worldwide. Most Phalaenopsis cultivars have long inflorescences that cause shipping problems and increase handling costs. Miniaturization of Phalaenopsis orchids not only reduces overall production costs but also can expand the appeal of the orchids to a different group of consumers who prefer to keep flowers on desks or tabletops. Although some miniature Phalaenopsis plants can be obtained via hybridization or mutation, they are unpredictable and limited in variety. We therefore used the transgenic approach of overexpressing gibberellin 2-oxidase 6 (OsGA2ox6), a rice GA deactivation gene, to investigate its functional effect in miniaturizing Phalaenopsis and to create a stable miniaturization platform to facilitate a supply for the potential demands of the miniature flower market.

Results: A commercial moth orchid, Phalaenopsis Sogo Yukidian 'SPM313', was transformed with the plasmid vector Ubi:OsGA2ox6 and successfully overexpressed the OsGA2ox6 gene in planta. The transgenic lines displayed darker-green, shorter, and wider leaves, thicker roots and much shorter flower spikes (10 cm vs 33 cm) than the nontransgenic line with a normal flower size and blooming ability and are therefore an ideal miniaturized form of Phalaenopsis orchids.

Conclusions: We demonstrated that the ectopic expression of OsGA2ox6 can miniaturize Phalaenopsis Sogo Yukidian 'SPM313' while preserving its blooming ability, providing an alternative, useful method for miniaturizing Phalaenopsis species. This miniaturization by a transgenic approach can be further expanded by using GA2ox genes from different plant species or different gene variants, thereby expanding the technical platform for miniaturizing Phalaenopsis species to meet the potential demands of the miniature Phalaenopsis flower market.

Abstract Image

Abstract Image

Abstract Image

水稻ga2氧化酶基因OsGA2ox6的过表达使蝴蝶兰小型化。
背景:蝴蝶兰是世界上最常见的盆栽兰花之一。大多数蝴蝶兰品种的花序都很长,这会造成运输问题,增加处理成本。蝴蝶兰的小型化不仅降低了整体生产成本,而且可以扩大兰花对喜欢将花放在桌子或桌面的不同消费者群体的吸引力。虽然一些小型蝴蝶兰植物可以通过杂交或突变获得,但它们是不可预测的,品种有限。因此,我们采用过表达水稻赤霉素(GA)失活基因赤霉素2-氧化酶6 (OsGA2ox6)的转基因方法,研究其在蝴蝶兰微型化中的功能作用,并建立稳定的微型化平台,为微型化花卉市场的潜在需求提供保障。结果:用质粒载体Ubi:OsGA2ox6转化商品蛾兰蝴蝶兰(Phalaenopsis Sogo Yukidian 'SPM313'),成功在植物中过表达OsGA2ox6基因。与具有正常花大小和开花能力的非转基因品系相比,转基因品系呈现出深绿色、更短、更宽的叶片、更粗的根和更短的花穗(10厘米对33厘米),因此是理想的蝴蝶兰微缩型品种。结论:我们证明了OsGA2ox6的异位表达可以使蝴蝶兰Sogo Yukidian 'SPM313'小型化,同时保持其开花能力,为蝴蝶兰物种小型化提供了另一种有用的方法。利用不同植物物种或不同基因变异的gaox基因,可以进一步扩大这种转基因方式的小型化,从而扩大蝴蝶兰品种小型化的技术平台,满足微型蝴蝶兰花卉市场的潜在需求。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Botanical Studies
Botanical Studies PLANT SCIENCES-
CiteScore
4.80
自引率
2.90%
发文量
32
审稿时长
13 weeks
期刊介绍: Botanical Studies is an open access journal that encompasses all aspects of botany, including but not limited to taxonomy, morphology, development, genetics, evolution, reproduction, systematics, and biodiversity of all plant groups, algae, and fungi. The journal is affiliated with the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan.
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