Malaysian Journal of Pathology最新文献_第8页

The correlation of EMT and p53 immunohistochemical markers with cisplatin resistance in muscle invasive bladder cancer patients: A single-centred study. 肌浸润性膀胱癌患者EMT和p53免疫组织化学标志物与顺铂耐药的相关性:一项单中心研究

IF 1.8 4区医学

Malaysian Journal of Pathology Pub Date : 2023-04-01

Y Paramanantham, N A B M Said, K S Mun

{"title":"The correlation of EMT and p53 immunohistochemical markers with cisplatin resistance in muscle invasive bladder cancer patients: A single-centred study.","authors":"Y Paramanantham, N A B M Said, K S Mun","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Although epithelial-mesenchymal transition (EMT) and p53 have been established to play a pivotal role in the aggressiveness of muscle-invasive bladder cancer (MIBC), its pathological correlation to cisplatin treatment in the Malaysian patient cohort is lacking. This study aimed to evaluate the association of EMT markers, e-cadherin, vimentin and actin, as well as tumour suppressor gene, p53, in cisplatin-receiving MIBC patients.Materials and methods: Formalin-fixed paraffinembedded (FFPE) blocks of muscle-invasive bladder cancer patients receiving cisplatin-based chemotherapy between January 2010 to December 2020 were traced. Immunohistochemistry staining was performed on traced blocks using antibodies to e-cadherin, vimentin and actin, and p53.Results: p53 and e-cadherin were stained positive in most cases (p=0.515 and 0.242 respectively), although e-cadherin showed stronger positive expression in pre-cisplatin receiving MIBC cases. All the cases stained negative for actin and vimentin except for faint staining observed in one pre-cisplatin case.Conclusion: Although this study does not show a significant correlation between EMT markers and p53 with cisplatin-responsiveness in MIBC patients, the results serve as preliminary findings on the heterogeneous outcomes of molecular staining in the Malaysian MIBC patient cohort.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"45 1","pages":"19-29"},"PeriodicalIF":1.8,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9861814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adamantinoma-like Ewing sarcoma of thyroid gland-An unfamiliar mimicker of epithelial and mesenchymal neoplasms of the head and neck. 甲状腺金刚素瘤样尤文氏肉瘤-一种不熟悉的头颈部上皮和间质肿瘤的模拟物。

IF 1.8 4区医学

Malaysian Journal of Pathology Pub Date : 2023-04-01

I Mohammed, B Baskovich, A Alkhasawneh, A Gopinath

引用次数: 0

The relationship between Pattern B lipoprotein profile and low-density lipoprotein cholesterol subfractions with Metabolic Syndrome and Framingham Risk Score in adults at health screening in Malaysia. 马来西亚成人健康筛查中B型脂蛋白谱和低密度脂蛋白胆固醇亚组分与代谢综合征和Framingham风险评分的关系

IF 1.8 4区医学

Malaysian Journal of Pathology Pub Date : 2023-04-01

S C Thambiah, P Mazalan, S Mohamad Ismuddin, I N Samsudin, G Appannah, S Md Said, S Y Zahari Sham

{"title":"The relationship between Pattern B lipoprotein profile and low-density lipoprotein cholesterol subfractions with Metabolic Syndrome and Framingham Risk Score in adults at health screening in Malaysia.","authors":"S C Thambiah, P Mazalan, S Mohamad Ismuddin, I N Samsudin, G Appannah, S Md Said, S Y Zahari Sham","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Traditional cardiovascular (CV) risk factors are constituents of Metabolic Syndrome (MetS) and Framingham Risk Score (FRS). However, CV risk exists even when these risk parameters are normal and have been attributed to the atherogenic small dense low-density lipoprotein cholesterol (sdLDL). This study aimed to determine the association of Pattern B and LDL subfractions with MetS and FRS among selected Malaysian population.Materials and methods: A cross-sectional study of 380 subjects ≥30 years old at health screening. Sociodemographic factors and clinical characteristics were recorded. Fasting serum lipids, LDL subfractions and plasma glucose were analysed.Results: Being older, Malay with Pattern B independently predicted MetS. Being male, Chinese with Pattern B and increased body mass index (BMI) and diastolic blood pressure (DBP) were more likely to be in the intermediate to high risk FRS group. Common independent biochemical predictors include LDL1 and sdLDL: LDL3 in MetS and non-high-density lipoprotein cholesterol in FRS.Conclusion: BMI and DBP may provide incremental prognostic value to FRS risk estimates if included. Considering a significant incidence of Pattern B in low FRS risk subjects (13.4%), routine LDL subfraction analysis could identify these individuals that would be overlooked if their risk were predicted solely based on their FRS only. The non-specific lowering of LDL1 by lipid-lowering therapy based on conventional lipid profile might have a negative effect on several physiological processes. Hence, if LDL subfractions are determined, therapy can be targeted towards sdLDL. Recognising asymptomatic individuals who carry high CV risk is pertinent in primary prevention.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"45 1","pages":"97-109"},"PeriodicalIF":1.8,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9366599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of quantitative point-of-care test for measurement of glucose-6-phosphate dehydrogenase enzyme activity in Malaysia. 评估定量点护理测试测量葡萄糖-6-磷酸脱氢酶活性在马来西亚。

IF 1.8 4区医学

Malaysian Journal of Pathology Pub Date : 2023-04-01

M A H Zailani, R Z A Raja Sabudin, D Abdul Jalil, A Ithnin, N A Ayub, H Alauddin, N Jalil, A Md Fauzi, F C Cheah, L S Lim, N Safian, M M Yusuf, A Othman

{"title":"Evaluation of quantitative point-of-care test for measurement of glucose-6-phosphate dehydrogenase enzyme activity in Malaysia.","authors":"M A H Zailani, R Z A Raja Sabudin, D Abdul Jalil, A Ithnin, N A Ayub, H Alauddin, N Jalil, A Md Fauzi, F C Cheah, L S Lim, N Safian, M M Yusuf, A Othman","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: The treatment of Plasmodium vivax malaria with 8-aminoquinolines is contraindicated in glucose-6-phosphate dehydrogenase (G6PD) deficient individuals due to the risk of acute haemolytic anaemia. Effective G6PD screening is paramount to avoid adverse drug reactions. This study aimed to evaluate the performance of novel quantitative point-of-care (POC) tests as a new screening method for G6PD deficiency in Malaysia.Materials and methods: A total of 153 neonatal cord blood, 99 peripheral blood of older children aged between 1 month to 12-years old, and 62 peripheral adult blood were screened for G6PD deficiency using two quantitative POC tests, CareStartTM biosensor (Carestart) and CareStartTM Biosensor 1 (S1). The results were compared with OSMMR2000D kit as a reference assay. Two statistical analyses were performed in this study to evaluate the POC test performances, the Spearman's correlation test and the Cohen's kappa method.Results: Both Carestart and S1 tests showed significant positive correlations to OSMMRS000D with r2 = 0.7916 and r2 = 0.7467. Their measurement of agreement showed a kappa (κ) value of 0.805 (p<0.001, 95% CI), and 0.795 (p<0.001, 95% CI), respectively. Analysis of the area under the Receiver Operating Curve (ROC) at 60% cut-off illustrated that the Carestart had 90.2% sensitivity, 98.9% specificity, 98.3% positive predictive value (PPV), and 93.8% negative predictive value (NPV). The corresponding values for the S1 were 95.2%, 100%, 100%, and 96.8%, respectively.Conclusion: This study showed that the Carestart and S1 biosensors have high-performance reliability for screening of G6PD deficiency, which can guide safe prescriptions of anti-malaria medications and hence, eradication of Plasmodium vivax malaria.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"45 1","pages":"31-41"},"PeriodicalIF":1.8,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9366603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mixed autoimmune haemolytic anaemia in a COVID-19 patient. 1例COVID-19患者的混合性自身免疫性溶血性贫血

IF 1.8 4区医学

Malaysian Journal of Pathology Pub Date : 2023-04-01

N Mohd Shukri, N A F A A Ab Aziz

引用次数: 0

Convalescent plasma transfusion in severe COVID-19 patients: Clinical and laboratory outcomes. 重症COVID-19患者恢复期血浆输血:临床和实验室结果

IF 1.8 4区医学

Malaysian Journal of Pathology Pub Date : 2023-04-01

M R Abdullah, A Alam Faizli, N S Adzaludin, N Abu Amin, N A Misnan, L L Low

{"title":"Convalescent plasma transfusion in severe COVID-19 patients: Clinical and laboratory outcomes.","authors":"M R Abdullah, A Alam Faizli, N S Adzaludin, N Abu Amin, N A Misnan, L L Low","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: The objective of this study was to investigate the effect of convalescent plasma (CP) transfusion on clinical and serial laboratory parameters in severe COVID-19 patients. The Coronavirus Disease 2019 (COVID-19) pandemic presents a challenge to the healthcare system worldwide due to the limited treatment options available. The body of evidence reported that CP containing anti- COVID-19 antibodies could be effective against the infection.Materials and methods: This was a cross-sectional study that involved retrospective data collection of severe COVID-19 adult patients who received CP transfusion along with the best-of-care (CP group, n: 53) and best-of-care only (control group, n: 53). An age, gender, and comorbidity were manually matched approximately at a 1:1 ratio.Results: The prevalence of adverse transfusion reactions was 5.7%. A shorter duration of oxygen support (median: 12 days vs 14 days, P=0.030) and a shorter duration of mechanical ventilation (median: 6 days vs 10 days, P=0.048) were found in the CP group. The laboratory parameters were also improved. However, there was no significant difference in the mechanical ventilation rate, length of hospital stay, length of intensive care unit (ICU) stay, and mortality rate across both groups (P = 0.492, 0.614, 0.793, 0.374).Conclusion: CP transfusion is safe and effective in the treatment of severe COVID-19 patients. However, a revision of our approaches such as early CP transfusion and use of a high-titre anti-COVID-19 neutralising antibody (nAb) unit is necessary to unlock the full potential benefits of CP transfusion among COVID-19 patients.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"45 1","pages":"87-95"},"PeriodicalIF":1.8,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9373816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of N-acetylcysteine on lung alveolar epithelial cells infected with respiratory syncytial virus. n -乙酰半胱氨酸对呼吸道合胞病毒感染肺泡上皮细胞的影响。

IF 1.8 4区医学

Malaysian Journal of Pathology Pub Date : 2023-04-01

K K Wong, K P Kua, K S Ooi, F C Cheah

{"title":"The effects of N-acetylcysteine on lung alveolar epithelial cells infected with respiratory syncytial virus.","authors":"K K Wong, K P Kua, K S Ooi, F C Cheah","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: Respiratory syncytial virus (RSV) is one of the most common causes of acute lower respiratory infection in infants and young children. Mucolytic agents, such as acetylcysteine and carbocysteine have reported benefits in alleviating acute upper or lower respiratory infections. Among these, N-acetylcysteine (NAC) has cyto-protective effects when cells are infected with the RSV.Materials and methods: Our study investigated primarily the dose-dependent effects of NAC on respiratory alveolar epithelial (A549) cells when co-cultured with RSV in vitro. Three different concentrations of NAC were used, 0.1 mM, 1 mM, and 10 mM. The cytotoxicity of RSV-infected cells was measured by lactate dehydrogenase and antiviral activity of NAC on cell cultures was evaluated by immunofluorescence.Results: Pre-treatment with the highest dose, 10 mM NAC, resulted in features of cell injury even without RSV infection. The proportion of cells infected by RSV and RSV-induced cell death decreased by more than 3-fold when cells were pre-treated with 1 mM NAC. Pre-treatment at the lowest dose, 0.1 mM, did not show any significant changes.Conclusion: A moderate dose of NAC (1 mM) appeared protective of RSV infection to lung alveolar epithelial cells. However, a higher dose of NAC (10 mM) may be relatively toxic and injurious to these cells.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"45 1","pages":"43-50"},"PeriodicalIF":1.8,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9373819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum separation abnormality in a multiple myeloma patient. 多发性骨髓瘤患者血清分离异常。

IF 1.8 4区医学

Malaysian Journal of Pathology Pub Date : 2023-04-01

M R Zaharudin, S C Thambiah, I N Samsudin, H A Hamid

引用次数: 0

Eosinophilic/ T cell chorionic vasculitis. 嗜酸性粒细胞/ T细胞绒毛膜血管炎。

IF 1.8 4区医学

Malaysian Journal of Pathology Pub Date : 2023-04-01

G C Tan, Y P Wong, R Abd Rahman

引用次数: 0

Seasonal variation of eosinophil counts in histologically normal colonic mucosal biopsies. 组织学正常的结肠粘膜活检中嗜酸性粒细胞计数的季节变化。

IF 1.8 4区医学

Malaysian Journal of Pathology Pub Date : 2023-04-01

S Elshebli, J Obeid, L Abudalu, A Albayati, M Abo Osba, O Alsadeq, H Awad

{"title":"Seasonal variation of eosinophil counts in histologically normal colonic mucosal biopsies.","authors":"S Elshebli, J Obeid, L Abudalu, A Albayati, M Abo Osba, O Alsadeq, H Awad","doi":"","DOIUrl":"","url":null,"abstract":"Introduction: The eosinophil counts in colonic biopsies are affected by geographical and possibly seasonal variations. This study aims to investigate the significance of seasonal variations of eosinophil counts in histologically normal colonic mucosal biopsies.Materials and methods: This is a retrospective, cross sectional study that included 337 cases of normal colonic biopsies. The number of eosinophils per high power field was counted in the most densely populated area. The eosinophilic counts were compared among genders, age groups, biopsy sites and in various months and seasons. Two tailed T-test was used to compare means and a p value < 0.05 was considered significant.Results: 173 (51%) of cases were from males. The age range was between 18-82 with the mean being 51.7 years (SD= 17.5). 181 (54%) biopsies were from the right colon and 156 (46%) from the left colon. There was a statistically significant difference between eosinophil counts in the right colon (mean 20.2, SD 13.2) and left colon (mean 13.8, SD10.1); p value <0.001. The mean eosinophil counts was highest in autumn (21.1) followed by spring (18.3). The counts in winter and summer were close (15.2 and 15.1 respectively). There was a statistically significant difference between counts in autumn and summer (p=0.013) and between autumn and winter (p=0.008). However, there was no statistically significant differences between autumn and spring counts (p=0.183). When stratified according to site, this pattern of statistical significance was observed in the right colon but not the left colonic mucosal biopsies.Conclusion: There are significant seasonal variations of eosinophil counts in normal colonic biopsies which are more pronounced in the right colon. Pathologists and gastroenterologists need to be aware of these variations and to take them into account when determining if a patient has tissue eosinophilia.","PeriodicalId":48723,"journal":{"name":"Malaysian Journal of Pathology","volume":"45 1","pages":"11-18"},"PeriodicalIF":1.8,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9373820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0