Mycokeys最新文献

{"title":"Taxonomy and phylogeny of entomopathogenic fungi from China-revealing two new genera and thirteen new species within Clavicipitaceae (Hypocreales, Ascomycota).","authors":"Zhi-Qin Wang, Zhi-Li Yang, Jing Zhao, Jin-Mei Ma, De-Xiang Tang, Zong-Li Liang, Jian-Hong Li, Xin-Mao Zhou, Hong Yu","doi":"10.3897/mycokeys.117.140577","DOIUrl":"10.3897/mycokeys.117.140577","url":null,"abstract":"<p><p>Scale insects (Coccidae, Hemiptera) and whiteflies (Aleyrodidae, Homoptera) are diminutive, ubiquitous, sap-sucking plant parasites, many of which are serious agricultural pests. Over the course of several years, an investigation into entomopathogenic fungi affecting scale insects and whiteflies resulted in the collection of 13 novel species of Clavicipitaceae in Yunnan and Hainan Provinces, China. Based on three-loci (nrLSU, <i>tef</i>-1<i>a</i>, and <i>rpb1</i>) phylogenetic analysis and morphological evidence, it was determined that two new genera, <i>Paramoelleriella</i> and <i>Polymicrospora</i>, each encompassed a new species. Additionally, two new species of <i>Hypocrella</i> <i>s. str.</i> and nine new species of <i>Moelleriella</i> were identified. Within the <i>Moelleriella</i> clade, seven new species were assigned to the Effuse clade and two to the Globose clade. <i>Hypocrella</i> s. str. and <i>Samuelsia</i> were included in the Pulvinate clade, to which the new genus <i>Paramoelleriella</i> is closely related, although it forms a distinct branch. <i>Paramoelleriella</i> species exhibited characteristics similar to those of <i>Moelleriella</i>, including globose to subglobose, yellow to orange teleomorphic stromata, with perithecia densely arranged and fully embedded in the stromatal tissue. Its ascospores disarticulated into short-cylindrical part-spores, and the conidiomata featured large, widely open orifices bearing fusoid conidia curved to one side. Species of the new genus <i>Polymicrospora</i> were characterized by thin-pulvinate, snow-white to off-white teleomorphic stromata with surface smooth. These species possessed numerous obpyriform or oval, semi-embedded, and densely arranged perithecia, cylindrical asci, and ascospores that disarticulated into small, oval part-spores in large quantities. This study introduces two new genera and 13 new species, accompanied by detailed illustrations and descriptions.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"121-169"},"PeriodicalIF":2.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive genome analysis of two <i>Cytospora</i> (Cytosporaceae, Diaporthales) species associated with canker disease of spruce: <i>C.piceae</i> and <i>C.piceicola</i> sp. nov.","authors":"Evgeny Ilyukhin, Yanpeng Chen, Svetlana Markovskaja, Ashwag Shami, Sajeewa S N Maharachchikumbura","doi":"10.3897/mycokeys.117.145445","DOIUrl":"10.3897/mycokeys.117.145445","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Cytospora canker (CC) is among the most important diseases in conifer trees (&lt;i&gt;Picea&lt;/i&gt; spp., mainly). This disease poses a significant risk factor for forest health, potentially leading to economic losses for wood producers. To provide a genomic basis of the CC pathogenesis, the genomes of two &lt;i&gt;Cytospora&lt;/i&gt; species associated with the disease were sequenced and further analyzed within a set of Diaporthales species. The first species was identified as &lt;i&gt;C.piceae&lt;/i&gt;. The second was described as &lt;i&gt;C.piceicola&lt;/i&gt; &lt;b&gt;sp. nov.&lt;/b&gt; based on morphological characteristics and multi-gene phylogenetic analysis. The novel species is sister to other &lt;i&gt;Cytospora&lt;/i&gt; species isolated from conifers. Here, we report 39.7 and 43.8 Mb highly contiguous genome assemblies of &lt;i&gt;C.piceae&lt;/i&gt; EI-19(A) and &lt;i&gt;C.piceicola&lt;/i&gt; EI-20, respectively, obtained using Illumina sequencing technology. Despite notably different genome sizes, these species share the main genome characteristics, such as predicted gene number (10,862 and 10,742) and assembly completeness (97.6% and 98.1%). A wide range of genes encoding carbohydrate-active enzymes, secondary metabolite biosynthesis clusters, and secreted effectors were found. Multiple experimentally validated virulence genes were also identified in the studied species. The defined arsenals of enzymes and effectors generally relate to the hemibiotrophic lifestyle with a capability to switch to biotrophy. The obtained evidence also supports that &lt;i&gt;C.piceae&lt;/i&gt; EI-19(A) and &lt;i&gt;C.piceicola&lt;/i&gt; EI-20 can cause severe canker disease symptoms in &lt;i&gt;Picea&lt;/i&gt; spp. specifically. It was additionally observed that the strains of &lt;i&gt;C.piceae&lt;/i&gt; may have different pathogenicity and virulence characteristics based on the analyses of predicted secondary metabolite complements, effectomes, and virulence-related genes. Phylogenomic analysis and timetree estimations indicated that divergence of the studied species may have occurred relatively late, 11-10 million years ago. Compared to other members of Diaporthales, &lt;i&gt;C.piceae&lt;/i&gt; EI-19(A) and &lt;i&gt;C.piceicola&lt;/i&gt; EI-20 implied a moderate rate of gene contraction, but the latter experienced significant gene loss that can additionally support host specificity attributed to these species. But uncovered gene contraction events may point out potential lifestyle differentiation and host shift of the studied species. It was revealed that EI-19(A) and &lt;i&gt;C.piceicola&lt;/i&gt; EI-20 carry distinct secretomes and effectomes among Diaporthales species. This feature can indicate a species lifestyle and pathogenicity potential. These findings highlight potential targets for identification and/or detection of pathogenic &lt;i&gt;Cytospora&lt;/i&gt; in conifers. The introduced draft genome sequences of &lt;i&gt;C.piceae&lt;/i&gt; and &lt;i&gt;C.piceicola&lt;/i&gt; can be employed as tools to understand basic genetics and pathogenicity mechanisms of fungal species causing canker disease in woody plants. The identified pathogenicity and viru","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"89-119"},"PeriodicalIF":2.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Paracorymbiglomus</i> gen. nov., <i>Diversisporaconica</i> sp. nov., and new combinations in Diversisporaceae (Glomeromycota).","authors":"Janusz Błaszkowski, Bruno Tomio Goto, Szymon Zubek, Paweł Milczarski, Ryszard Malinowski, Piotr Niezgoda, Tomasz Błaszkowski","doi":"10.3897/mycokeys.117.148052","DOIUrl":"10.3897/mycokeys.117.148052","url":null,"abstract":"<p><p>The paper presents the results of morphological studies, as well as comparisons and phylogenetic analyses of sequences of the 45S (= 18S-ITS-28S) nuc rDNA region and the <i>rpb1</i> gene (when available) of four arbuscular mycorrhizal fungi (AMF) of the phylum Glomeromycota. These fungi were (i) an informally named isolate 449, suspected of being an undescribed species of the genus <i>Diversispora</i>, and (ii) three <i>Corymbiglomus</i> species in the family Diversisporaceae. The studies confirmed the novelty of isolate 449 in <i>Diversispora</i> and showed that <i>Corymbiglomus</i> contains only <i>C.corymbiforme</i>, while <i>C.globiferum</i> and <i>C.pacificum</i> should be transferred to a separate genus sister to <i>Corymbiglomus</i>. Consequently, isolate 449 was described as <i>Diversisporaconica</i> <b>sp. nov.</b>, <i>C.globiferum</i>, and <i>C.pacificum</i> were placed in <i>Paracorymbiglomus</i> <b>gen. nov.</b> and renamed <i>P.globiferum</i> <b>comb. nov.</b> and <i>P.pacificum</i> <b>comb. nov.</b></p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"171-190"},"PeriodicalIF":2.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introducing <i>Muciflexusinthanonensis</i> gen. et sp. nov. and updates on <i>Ochronectria</i> (Hypocreales): New insights from leaf litter fungi in Doi Inthanon National Park, Northern Thailand.","authors":"Veenavee S Hittanadurage Silva, Ruvishika S Jayawardena, Rekhani H Perera, Qirui Li, Kevin D Hyde","doi":"10.3897/mycokeys.117.147002","DOIUrl":"10.3897/mycokeys.117.147002","url":null,"abstract":"<p><p>While exploring the leaf litter fungal diversity in Doi Inthanon National Park, Northern Thailand, we discovered a previously unknown lineage within Niessliaceae. <i>Muciflexusinthanonensis</i> <b>gen. et sp. nov.</b> is therefore introduced to accommodate this new lineage. The link between the sexual and asexual morphs of <i>Ochronectriathailandica</i> is also established, while the generic description of <i>Ochronectria</i> is updated. A polyphasic approach was employed, consisting of multi-locus phylogenetic analysis using ITS, LSU, <i>rpb2</i>, and <i>tef1-α</i> along with the morphological evidence to support the findings. <i>Muciflexusinthanonensis</i> resembles the spore morphology of <i>Phialoseptomonium</i> even though they are phylogenetically distant. <i>Muciflexusinthanonensis</i> forms a sister clade with <i>Trichonectriasetadpressa</i>, characterised by a long branch, but differs in asexual morphology, possessing clusters of simple or branched conidiophores compared to the sporodochia-like structure of <i>T.setadpressa</i>. The discovery of the asexual morph of <i>Ochronectriathailandica</i> from a terrestrial habitat confirms the versatile nature of the fungus, which inhabits both aquatic and terrestrial environments. Here we establish the link between the sexual and asexual morphs of <i>Ochronectria</i> by revealing the holomorphic nature of <i>O.thailandica</i>. This study highlights the diversity of leaf litter fungi and the potential of discovering novel fungal species in northern Thailand.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"67-88"},"PeriodicalIF":2.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and molecular analyses revealed four new wood-inhabiting fungal species (Hymenochaetales, Basidiomycota) from Yunnan.","authors":"Yinglian Deng, Meng Chen, Linfeng Liu, Qizhen Li, Sicheng Zhang, Haisheng Yuan, Changlin Zhao","doi":"10.3897/mycokeys.117.146236","DOIUrl":"https://doi.org/10.3897/mycokeys.117.146236","url":null,"abstract":"<p><p>Hymenochaetales is one of the fungal orders mainly composed of wood-inhabiting macrofungi within the class Agaricomycetes, Basidiomycota. Four new Hymenochaetales wood-inhabiting fungi, <i>Hymenochaetebannaensis</i>, <i>Lyomycesasiaticus</i>, <i>Peniophorellaalbohymenia</i>, and <i>P.punctata</i> collected from China are proposed based on morphological characteristics and molecular evidence. <i>H.bannaensis</i> is distinguished by flocculent basidiomata with cinnamon to yellowish brown to rust-brown hymenial surface, generative hyphae with simple septa and broadly ellipsoid to globose basidiospores. <i>L.asiaticus</i> is characterized by the membranaceous basidiomata with white to cream hymenial surface with tuberculate, a monomitic hyphal system with clamped generative hyphae and ellipsoid basidiospores. In addition, <i>P.albohymenia</i> is delimited by membranaceous basidiomata with white hymenial surface, four types of cystidia as stephanocyst, fusiform, cylindrical and capitate, and ellipsoid basidiospores. <i>P.punctata</i> is unique in the membranaceous, punctate basidiomata with white to pale yellow hymenial surface, fusiform cystidia, and allantoid basidiospores. Sequences of ITS and nLSU rRNA markers based on phylogenetic analyses were performed using the Maximum Likelihood, Maximum Parsimony and Bayesian Inference methods.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"29-66"},"PeriodicalIF":2.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morpho-molecular analyses reveal two novel species and two new records of Dictyosporiaceae (Pleosporales) from Dulongjiang River in northwestern Yunnan Province, China.","authors":"Ying Wang, D Jayarama Bhat, Dan-Feng Bao, Hong-Wei Shen, Yuan Feng, Zong-Long Luo","doi":"10.3897/mycokeys.117.145587","DOIUrl":"https://doi.org/10.3897/mycokeys.117.145587","url":null,"abstract":"<p><p>Yunnan Province is rich in freshwater resources, providing a favorable environment for freshwater fungi. As a result, numerous new species have been described in recent years, further supporting the extensive diversity of freshwater fungi in this region. During an investigation of lignicolous freshwater fungi from the Dulongjiang River, six fresh isolates of Dictyosporiaceae were obtained. The new collection includes two new species (<i>Dictyocheirosporasplendida</i> and <i>Jalapriyaguttulata</i>), two new records (<i>D.nabanheensis</i> and <i>Dictyosporiumhughesii</i>), and one known species (<i>Dictyosporiumduliujiangense</i>). They are introduced based on morphological evidence and molecular phylogenetic analysis of combined ITS, LSU, and <i>tef</i>1-α sequence data. Detailed descriptions and illustrations of these species are provided, and morphological comparison with similar taxa is discussed.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"1-27"},"PeriodicalIF":2.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144046648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new species of <i>Penicillium</i> and a new genus in <i>Xylariomycetidae</i> from the forest dump-sites in Chiang Mai, Thailand.","authors":"Tanapol Thitla, Jutamart Monkai, Weiqian Meng, Surapong Khuna, Ning Xie, Sinang Hongsanan, Saisamorn Lumyong","doi":"10.3897/mycokeys.116.150635","DOIUrl":"https://doi.org/10.3897/mycokeys.116.150635","url":null,"abstract":"<p><p>Waste accumulation in forest regions can have a severe impact on the soil mycobiome. However, research on soil fungi inhabiting forest disposal sites remains limited. Therefore, this study focused on the taxonomy and phylogeny of ascomycetes isolated from soil in forest dump-sites in Chiang Mai, Thailand. The fungal strains were identified using morphological characterisations and multigene phylogenetic reconstruction. A new genus, <i>Pseudoleptodontidium</i>, typified by <i>Ps.chiangmaiense</i> <b>sp. nov.</b> (Amphisphaeriales genera <i>incertae sedis</i>, Xylariomycetidae), along with two new species, <i>Penicilliumchiangmaiense</i> (series <i>Janthinella</i>, section Lanata-Divaricata) and <i>P.terrae</i> (series <i>Erubescentia</i>, section Exilicaulis) (Aspergillaceae, Eurotiales), are described in detail and compared with closely-related species. Our discovery offers valuable insights into the soil ascomycetes associated with forest disturbances.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"116 ","pages":"275-301"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12056519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Biconidiumsinense</i> gen. et sp. nov. (Hypocreales, Bionectriaceae) and <i>Didymocyrtisshanxiensis</i> sp. nov. (Phaeosphaeriaceae, <i>Didymocyrtis</i>) isolated from urban soil in China.","authors":"Hai-Yan Wang, Chunbo Dong, Yan-Wei Zhang, Wan-Hao Chen, Yan-Feng Han","doi":"10.3897/mycokeys.116.146683","DOIUrl":"https://doi.org/10.3897/mycokeys.116.146683","url":null,"abstract":"<p><p>During a fungal diversity survey in various urban habitats across China, 5 fungal isolates were discovered from soil samples. Detailed morphological observations and multi-gene phylogenetic analyses confirmed the identification of two novel taxa: <i>Biconidiumsinense</i> <b>gen. et sp. nov.</b> and <i>Didymocyrtisshanxiensis</i> <b>sp. nov.</b> These species were formally described, illustrated, and discussed, highlighting their distinct characteristics and taxonomic placement. The study expands our understanding of fungal diversity in urban environments, emphasizing the importance of combining morphological and molecular approaches for accurate species delineation and discovery.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"116 ","pages":"327-344"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12056516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Four new species of <i>Entoloma</i> subgenus <i>Cyanula</i> (Entolomataceae, Agaricales) from subtropical regions of China.","authors":"Lin-Gen Chen, Hong Chen, Ling Ding, Yu-Qin Xu, Hui Zeng, Sheng-Nan Wang, Jun-Qing Yan","doi":"10.3897/mycokeys.116.145568","DOIUrl":"https://doi.org/10.3897/mycokeys.116.145568","url":null,"abstract":"<p><p>In this study, four species of EntolomasubgenusCyanula (<i>E.orientosinense</i>, <i>E.subgriseosquamulosum</i>, <i>E.subpraegracile</i>, and <i>E.wuyishanense</i>) from subtropical regions of China, are described as new to science based on morphological and phylogenetic analyses. Morphologically, <i>E.orientosinense</i> is characterized by the white basidiomata, relatively large basidiospores, and carneogriseum-type lamellae edge; <i>E.subgriseosquamulosum</i> is recognized by the fuscous pileus, crowded and adnate lamellae, and medium-sized basidiospores; <i>E.subpraegracile</i> is identified by the yellow pileus and intervenose lamellae with sterile or heterogeneous edge; <i>E.wuyishanense</i> is distinct by the blue basidiomata and fertile lamellae edge with slightly bluish pigmentation near the stipe. <i>Entolomaorientosinense</i> belongs to sect. Caesiocincta, subsect. Queletia, and <i>E.wuyishanense</i> belongs to sect. Poliopodes. The remaining two species each form independent branches and do not belong to any known sections. Detailed descriptions, color photos, and a key to related species are presented.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"116 ","pages":"303-325"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12056515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new species of <i>Penicillium</i> (Eurotiales, Aspergillaceae) from China based on morphological and molecular analyses.","authors":"Rui-Na Liang, Xiang-Hao Lin, Miao-Miao An, Guo-Zhu Zhao","doi":"10.3897/mycokeys.116.149376","DOIUrl":"https://doi.org/10.3897/mycokeys.116.149376","url":null,"abstract":"<p><p><i>Penicillium</i> is a large and significant genus of fungi, exhibiting widespread distribution across diverse substrates. Ongoing taxonomic and nomenclatural revisions have led to an annual increase in the number of newly described species. This study described two new <i>Penicillium</i> species, i.e., <i>P.lentum</i> and <i>P.tibetense</i>, discovered in China. They have been identified and characterized through morphological examination and both single gene and multigene phylogenetic analyses. Based on these analyses, <i>P.lentum</i> was classified within the section Brevicompacta, while <i>P.tibetense</i> was placed in the section Lanata-Divaricata. Both species exhibited the morphological features typical of their respective sections. <i>Penicilliumlentum</i> is characterized by restricted growth with dense colonies on agar media and predominantly generates terverticillate conidiophores. <i>Penicilliumtibetense</i> demonstrates rapid growth on media and has vigorous growth on CYA at 30 °C, producing biverticillate conidiophores. Comprehensive descriptions and detailed illustrations of these new species were presented. A morphological comparison between the new species and their closely related taxa was provided.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"116 ","pages":"255-274"},"PeriodicalIF":2.8,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}