Mycokeys最新文献

{"title":"Morphological and molecular identification of two new wood-inhabiting species (<i>Russulales</i>, <i>Basidiomycota</i>) from China.","authors":"Changlin Zhao, Qi Li, Xiangfu Liu","doi":"10.3897/mycokeys.131.192179","DOIUrl":"https://doi.org/10.3897/mycokeys.131.192179","url":null,"abstract":"<p><p>Wood-inhabiting fungi represent a highly diverse group of microorganisms that play essential roles in forest ecosystems. However, the species diversity of wood-inhabiting fungal taxa in the order <i>Russulales</i> has not been systematically documented in the high-altitude forests of Yunnan Province, China. In the present study, the detailed morphological characteristics and combined multi-locus phylogeny of the internal transcribed spacers (ITS) and the large subunit (nrLSU) of the nuclear ribosomal DNA (rDNA) revealed two new species in <i>Russulales</i>, viz., <i>Gloeopeniophorella luteola</i> and <i>Scytinostroma tenuissimum</i>. <i>Gloeopeniophorella luteola</i> is characterized by its membranaceous, thin basidiomata with a smooth hymenial surface, a monomitic hyphal system with simple septa, generative hyphae, and gloeocystidia, and thick-walled basidiospores measuring 4-4.9 × 3.8-4.4 µm. <i>Scytinostroma tenuissimum</i> is characterized by its soft membranaceous basidiomata with a cream to pale pink hymenial surface, a dimitic hyphal system with clamped generative hyphae, and ellipsoid basidiospores measuring 10.1-13.2 × 7.1-9.3 µm. Phylogenetic analysis based on the concatenated ITS + nLSU dataset demonstrated that the two new species grouped within the order <i>Russulales</i>, in which <i>G. luteola</i> grouped into the family <i>Russulaceae</i>, while <i>S. tenuissimum</i> grouped into the family <i>Peniophoraceae</i>, and <i>G. luteola</i> was sister to <i>G. bambusicola</i>, and <i>S. tenuissimum</i> grouped together with <i>S. acystidiatum</i> and <i>S. bambusinum</i>. Comprehensive descriptions, micrographs, and phylogenetic analyses of the two new species are provided.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"131 ","pages":"265-286"},"PeriodicalIF":3.6,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13139860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147844808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and phylogenetic evidence reveal two new <i>Pseudohydnum</i> (<i>Auriculariales</i>, <i>Basidiomycota</i>) species from Guangxi and Xizang, China.","authors":"Xin Zhang, Hong-Min Zhou, Zhe-Hui Tu, Guang-Yu Zeng, Yu-Cheng Dai","doi":"10.3897/mycokeys.131.189424","DOIUrl":"https://doi.org/10.3897/mycokeys.131.189424","url":null,"abstract":"<p><p><i>Pseudohydnum (Auriculariales)</i> is characterized by gelatinous, hydnoid basidiomata and longitudinally septate basidia. Currently, 26 species and one subspecies are accepted worldwide with molecular evidence, including two new species described here. In this study, specimens from Guangxi, Xizang, and Jilin provinces, China, were examined using morphological and phylogenetic analyses based on ITS1-5.8S-ITS2-nLSU sequences. The phylogeny resolves the genus as a well-supported monophyletic group. <i>Pseudohydnum umbrosum</i> is newly recorded from China. Two new species are described and illustrated as <i>P. fusiformis</i> from Xizang, characterized by olivaceous buff to clay-pink basidiomata, association with <i>Abies</i> at high elevations (above 3200 m), and the presence of fusiform cystidia-a feature previously known only from <i>P. cystidiatum</i> within the genus; and <i>P. rhododendri</i> from Guangxi, characterized by shell-shaped basidiomata with a pale mouse-gray pileal surface, the presence of cystidioles, and growing on living <i>Rhododendron</i>. The discovery of these new taxa and the new record further highlight China as a significant diversity center for <i>Pseudohydnum</i>.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"131 ","pages":"251-264"},"PeriodicalIF":3.6,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147822583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new species of <i>Tricholomopsis</i> (Agaricales) from China based on molecular phylogenetic and morphological evidence.","authors":"Gengshen Wang, Ting Guo, Xuan Chen, Jinpeng Liao, Yunrui Ma, Cong Huang, Cuixin Li","doi":"10.3897/mycokeys.131.185587","DOIUrl":"https://doi.org/10.3897/mycokeys.131.185587","url":null,"abstract":"<p><p><i>Tricholomopsis</i> is a genus of tricholomoid fungi, typically saprotrophic, characterized by a fibrillose to squamulose pileus, yellow lamellae, and prominent cheilocystidia. In this study, two new species of <i>Tricholomopsis</i>, namely <i>T. roseorubra</i> and <i>T. cortinata</i>, are described based on morphological characteristics and phylogenetic analyses of ITS-28S sequences. <i>Tricholomopsis roseorubra</i> is characterized by purplish-red, squamulose basidiomata and prominent cheilocystidia forming a sterile gill edge, whereas <i>T. cortinata</i> is distinguished by small basidiomata, globose basidiospores, and a well-developed cortinate annulus. The latter represents the first record of a cortinate annulus in <i>Tricholomopsis</i>.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"131 ","pages":"235-249"},"PeriodicalIF":3.6,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147822558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new soil-inhabiting fungi, <i>Malbranchea cavernicola</i> (Onygenales, Malbrancheaceae) and <i>Talaromyces pangmaphaensis</i> (Eurotiales, Trichocomaceae), from a cave in northern Thailand.","authors":"Gui-Qing Zhang, Nakarin Suwannarach, Terd Disayathanoowat, Rasmi Shoocongdej, Varis Domethong, Dong-Qin Dai, Rui-Meng Liu, Nalin N Wijayawardene, Jaturong Kumla","doi":"10.3897/mycokeys.131.188256","DOIUrl":"https://doi.org/10.3897/mycokeys.131.188256","url":null,"abstract":"<p><p>Caves are globally recognised as reservoirs of high fungal diversity, with cave soils harbouring many previously underexplored taxa. Fungal research in Thailand has been conducted for more than two decades; however, studies focusing on fungal diversity in cave environments remain limited. In the present study, six fungal strains were isolated from soil samples collected from the prehistoric Phi Man Long Long Rak Cave located in Pang Mapha District, Mae Hong Son Province, northern Thailand. These strains were identified, based on morphological characteristics and multi-locus phylogenetic analyses using sequences of the internal transcribed spacer (ITS), the nuclear ribosomal large subunit (LSU), β-tubulin (<i>BenA</i>), calmodulin (<i>CaM</i>) and the RNA polymerase II second largest subunit (<i>RPB</i>2). Four strains representing two new species, <i>Malbranchea cavernicola</i> and <i>Talaromyces pangmaphaensis</i>, are described herein. In addition, two strains identified as <i>Malbranchea gymnoascoides</i> are reported for the first time from soil and represent a new geographical record in Thailand. Full descriptions, illustrations and a phylogenetic tree showing the phylogenetic positions of the cave fungi identified in this study are provided.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"131 ","pages":"211-234"},"PeriodicalIF":3.6,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13129548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147822566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new species of <i>Neohelicomyces</i> (<i>Tubeufiaceae</i>, <i>Tubeufiales</i>) from southern China revealed by morphological and multi-locus phylogenetic analyses.","authors":"Li-Juan Zhang, Song Bai, Jian Ma, Yong-Zhong Lu","doi":"10.3897/mycokeys.131.180862","DOIUrl":"https://doi.org/10.3897/mycokeys.131.180862","url":null,"abstract":"<p><p>During a survey of helicosporous hyphomycetes in tropical regions of China, four fungal strains were isolated from decaying wood in freshwater habitats of Hainan Province. Multi-locus phylogenetic analyses (LSU, ITS, <i>tef</i>1-α, and <i>rpb</i>2), coupled with detailed morphological examinations, support the recognition of two new species, <i>Neohelicomyces baochengensis</i> and <i>N. xiangshuiensis</i>. Comprehensive morphological descriptions, illustrations, taxonomic notes, and phylogenetic evidence are provided to clarify their systematic placement. These findings broaden the current understanding of <i>Neohelicomyces</i> diversity and provide further records of the genus from tropical freshwater habitats in Hainan Province.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"131 ","pages":"195-210"},"PeriodicalIF":3.6,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147822529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morpho-phylogenetic evidence reveals novel species (Ascomycota) and new records from the Kunming World Horticultural Expo Garden in Yunnan, China.","authors":"Wenli Li, Rajesh Jeewon, Guilan Zhang, Huan Luo, Liting Zhao, Zhiqin Wang, Changlin Zhao, Hongmin Zhou","doi":"10.3897/mycokeys.131.185896","DOIUrl":"https://doi.org/10.3897/mycokeys.131.185896","url":null,"abstract":"<p><p>Fungi represent a kingdom of life with vast, undiscovered diversity. In the unique man-made ecosystem of the Kunming World Horticultural Expo Garden, a site with remarkable plant diversity, four fungal strains were isolated from diseased branches of two widely cultivated plants, <i>Olea europaea</i> (olive) and Nandina domestica. Through a comprehensive analysis integrating morphological characteristics and multi-locus phylogenetic data (including LSU, SSU, ITS, <i>rpb2</i>, <i>tef1-α</i>, and <i>tub2</i> sequences), we identified two new species: <i>Oedohysterium oleae</i> and <i>Melomastia kunmingensis</i>, both of which are phylogenetically and morphologically distinct from known species. Furthermore, we report <i>M. kevinhydei</i> as a new host record on <i>O. europaea</i>, suggesting a broader host range for this species, and, for the first time, document the sexual morph of the type species <i>Parapyrenochaeta protearum</i>, providing crucial morphological insights for this genus, previously known only in its asexual state. These findings underscore the immense value of unique horticultural environments as reservoirs of fungal diversity.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"131 ","pages":"169-194"},"PeriodicalIF":3.6,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13109736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147786458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Inocybaceae</i> of Pakistan: a checklist and description of two novel <i>Inocybe</i> species using morphological and multi-locus phylogenetic evidence.","authors":"Sana, Munazza Kiran, Frazana Rafique, Irina Druzhinina, Vasco Fachada","doi":"10.3897/mycokeys.131.179438","DOIUrl":"https://doi.org/10.3897/mycokeys.131.179438","url":null,"abstract":"<p><p>The family <i>Inocybaceae</i> (<i>Agaricales</i>, <i>Basidiomycota</i>) comprises ecologically important ectomycorrhizal fungi widely distributed across temperate and tropical forests. Pakistan hosts a diverse representation of this family, particularly species of the genus <i>Inocybe</i>. This study presents a comprehensive checklist of members of the family <i>Inocybaceae</i> reported from Pakistan. Based on the latest published data, the country contains 55 species of <i>Inocybaceae</i>, including 28 of the genus <i>Inocybe</i>, six of <i>Inosperma</i>, ten of <i>Mallocybe</i>, and 11 of <i>Pseudosperma</i>, while no records of <i>Auritella</i>, <i>Nothocybe</i>, or <i>Tubariomyces</i> have been documented in the country to date. Additionally, two new species, <i>Inocybe khalidii</i> and <i>Inocybe floribundae</i>, are described herein based on integrated morphological and multigene phylogenetic analyses. Phylogenetically, <i>I. khalidii</i> forms a distinct and strongly supported clade within the genus, confirming its relationship to allopatric species from Mexico, Japan, and the USA, while <i>I. floribundae</i> forms a well-supported clade within the Xanthomelas group, sister to European <i>I. corsica</i> and <i>I. diabolica</i>. Basidiomata of <i>I. khalidii</i> are differentially characterized by a vivid orange-brown subglabrous pileus, large and broadly ellipsoid to ellipsoid basidiospores, and a near absence of caulocystidia. Basidiomata of <i>I. floribundae</i> are morphologically distinct from their phylogenetic relatives by the beige to brown, distinctly scaly pileus, bulbous but weakly marginate stipe, apically restricted caulocystidia, utriform-obclavate hymenial cystidia, and subisodiametric, bluntly nodulose basidiospores. Morphological descriptions, illustrations, micrographs, and sequences of ITS+LSU- and <i>tef</i>1-based phylogenetic analyses are presented in this article with reference to the newly described species.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"131 ","pages":"115-148"},"PeriodicalIF":3.6,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13109739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147786420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology and molecular phylogeny reveal new species of <i>Sporocadus</i> (<i>Sporocadaceae</i>, <i>Amphisphaeriales</i>) in Xizang, China.","authors":"Zixuan Li, Chengming Tian, Ning Jiang","doi":"10.3897/mycokeys.131.187025","DOIUrl":"https://doi.org/10.3897/mycokeys.131.187025","url":null,"abstract":"<p><p><i>Sporocadus</i> is the type genus of <i>Sporocadaceae</i> and is phylogenetically closely related to <i>Seimatosporium</i>. Traditionally, these two genera were distinguished by the presence or absence of conidial appendages; however, this morphological character is sometimes plastic and unstable. Previous studies have also shown that internal transcribed spacer (ITS) and large subunit (LSU) sequence data are insufficient for their resolution. In this study, fungal specimens were collected from branches of two rosaceous hosts, <i>Prunus cerasifera</i> and <i>Rosa chinensis</i>, in Xizang, China. Based on morphological examination and multi-locus phylogenetic analyses of a combined ITS, LSU, <i>rpb2</i>, <i>tef1</i>, and <i>tub2</i> dataset, two new species are revealed and described herein as <i>Sp. bomiensis</i> and <i>Sp. prunicola</i> <b>spp. nov</b>. Furthermore, <i>Seimatosporium parvum</i>, which possesses distinct conidial appendages, is transferred to <i>Sporocadus</i> as <i>Sp. parvus</i> <b>comb. nov</b>. based on strong molecular evidence. Given the limited resolution of ITS and LSU and the instability of conidial appendages, we suggest that five-loci datasets including all accepted species are essential for accurate identification within the <i>Sporocadus</i>-<i>Seimatosporium</i> complex. This study not only expands the species diversity of <i>Sporocadus</i> in high-altitude regions but also provides new insights into the taxonomy and identification of pestalotioid fungi.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"131 ","pages":"149-168"},"PeriodicalIF":3.6,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13109733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147786475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and phylogenetic analyses reveal three new species of Chaetosphaeriaceae from China.","authors":"Sen Chen, Haimei Zhao, Wen Zheng, Long Han","doi":"10.3897/mycokeys.131.187694","DOIUrl":"https://doi.org/10.3897/mycokeys.131.187694","url":null,"abstract":"<p><p>During an ongoing taxonomic investigation of microfungi in southern China, three novel taxa belonging to the family Chaetosphaeriaceae are introduced based on morphological characteristics and phylogenetic analyses of combined ITS, LSU and <i>tef</i>1-α sequence data. Two of the new species, <i>Brunneodinemasporium septatum</i> and <i>Codinaea yulinensis</i>, possess phialidic asexual morphs, whereas the third new species, <i>Pseudolomaantha yulinensis</i>, is characterized by a non-phialidic, sporidesmium-like asexual morph. Detailed morphological descriptions and illustrations are provided for all taxa, together with notes comparing them to morphologically similar species. Phylogenetic analyses support their placement within Chaetosphaeriaceae and confirm their status as distinct species.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"131 ","pages":"99-114"},"PeriodicalIF":3.6,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13107140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147786518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pleopunctum xizangense</i> sp. nov. (Pleosporales, Phaeoseptaceae) and <i>Pseudolophiostoma yunnanense</i> sp. nov. (Pleosporales, Lophiostomataceae), and a new record of <i>Pleopunctum pseudoellipsoideum</i> on Fagales.","authors":"Yan-Yan Yang, Tai-Shun Li, Vinodhini Thiyagaraja, Chitrabhanu S Bhunjun, Shu-Cheng He, Pattana Kakumyan, Kevin D Hyde, Qi Zhao","doi":"10.3897/mycokeys.131.175264","DOIUrl":"https://doi.org/10.3897/mycokeys.131.175264","url":null,"abstract":"<p><p>Pleosporales is the largest order of Dothideomycetes and is widely distributed in terrestrial and aquatic environments. During a survey of saprobic fungi in southwestern China, we collected and isolated some Pleosporales taxa. Based on morphological comparisons and multi-gene (SSU-ITS-LSU-<i>tef1</i>) phylogenetic analyses, we describe two new species, <i>Pleopunctum xizangense</i> and <i>Pseudolophiostoma yunnanense</i>, as well as a new host record, <i>Pleopunctum pseudoellipsoideum</i>, that were collected from the plant family Fagales. <i>Pseudolophiostoma yunnanense</i> was found in its asexual state, the first such account for this genus. In this study, we compare and discuss morphologically similar species and phylogenetically related taxa, providing comprehensive illustrations and descriptions of the isolates.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"131 ","pages":"77-98"},"PeriodicalIF":3.6,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13107142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147786399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}