MycokeysPub Date : 2025-05-05eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.117.147002
Veenavee S Hittanadurage Silva, Ruvishika S Jayawardena, Rekhani H Perera, Qirui Li, Kevin D Hyde
{"title":"Introducing <i>Muciflexusinthanonensis</i> gen. et sp. nov. and updates on <i>Ochronectria</i> (Hypocreales): New insights from leaf litter fungi in Doi Inthanon National Park, Northern Thailand.","authors":"Veenavee S Hittanadurage Silva, Ruvishika S Jayawardena, Rekhani H Perera, Qirui Li, Kevin D Hyde","doi":"10.3897/mycokeys.117.147002","DOIUrl":"10.3897/mycokeys.117.147002","url":null,"abstract":"<p><p>While exploring the leaf litter fungal diversity in Doi Inthanon National Park, Northern Thailand, we discovered a previously unknown lineage within Niessliaceae. <i>Muciflexusinthanonensis</i> <b>gen. et sp. nov.</b> is therefore introduced to accommodate this new lineage. The link between the sexual and asexual morphs of <i>Ochronectriathailandica</i> is also established, while the generic description of <i>Ochronectria</i> is updated. A polyphasic approach was employed, consisting of multi-locus phylogenetic analysis using ITS, LSU, <i>rpb2</i>, and <i>tef1-α</i> along with the morphological evidence to support the findings. <i>Muciflexusinthanonensis</i> resembles the spore morphology of <i>Phialoseptomonium</i> even though they are phylogenetically distant. <i>Muciflexusinthanonensis</i> forms a sister clade with <i>Trichonectriasetadpressa</i>, characterised by a long branch, but differs in asexual morphology, possessing clusters of simple or branched conidiophores compared to the sporodochia-like structure of <i>T.setadpressa</i>. The discovery of the asexual morph of <i>Ochronectriathailandica</i> from a terrestrial habitat confirms the versatile nature of the fungus, which inhabits both aquatic and terrestrial environments. Here we establish the link between the sexual and asexual morphs of <i>Ochronectria</i> by revealing the holomorphic nature of <i>O.thailandica</i>. This study highlights the diversity of leaf litter fungi and the potential of discovering novel fungal species in northern Thailand.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"67-88"},"PeriodicalIF":2.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphological and molecular analyses revealed four new wood-inhabiting fungal species (Hymenochaetales, Basidiomycota) from Yunnan.","authors":"Yinglian Deng, Meng Chen, Linfeng Liu, Qizhen Li, Sicheng Zhang, Haisheng Yuan, Changlin Zhao","doi":"10.3897/mycokeys.117.146236","DOIUrl":"https://doi.org/10.3897/mycokeys.117.146236","url":null,"abstract":"<p><p>Hymenochaetales is one of the fungal orders mainly composed of wood-inhabiting macrofungi within the class Agaricomycetes, Basidiomycota. Four new Hymenochaetales wood-inhabiting fungi, <i>Hymenochaetebannaensis</i>, <i>Lyomycesasiaticus</i>, <i>Peniophorellaalbohymenia</i>, and <i>P.punctata</i> collected from China are proposed based on morphological characteristics and molecular evidence. <i>H.bannaensis</i> is distinguished by flocculent basidiomata with cinnamon to yellowish brown to rust-brown hymenial surface, generative hyphae with simple septa and broadly ellipsoid to globose basidiospores. <i>L.asiaticus</i> is characterized by the membranaceous basidiomata with white to cream hymenial surface with tuberculate, a monomitic hyphal system with clamped generative hyphae and ellipsoid basidiospores. In addition, <i>P.albohymenia</i> is delimited by membranaceous basidiomata with white hymenial surface, four types of cystidia as stephanocyst, fusiform, cylindrical and capitate, and ellipsoid basidiospores. <i>P.punctata</i> is unique in the membranaceous, punctate basidiomata with white to pale yellow hymenial surface, fusiform cystidia, and allantoid basidiospores. Sequences of ITS and nLSU rRNA markers based on phylogenetic analyses were performed using the Maximum Likelihood, Maximum Parsimony and Bayesian Inference methods.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"29-66"},"PeriodicalIF":2.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycokeysPub Date : 2025-04-30eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.117.145587
Ying Wang, D Jayarama Bhat, Dan-Feng Bao, Hong-Wei Shen, Yuan Feng, Zong-Long Luo
{"title":"Morpho-molecular analyses reveal two novel species and two new records of Dictyosporiaceae (Pleosporales) from Dulongjiang River in northwestern Yunnan Province, China.","authors":"Ying Wang, D Jayarama Bhat, Dan-Feng Bao, Hong-Wei Shen, Yuan Feng, Zong-Long Luo","doi":"10.3897/mycokeys.117.145587","DOIUrl":"https://doi.org/10.3897/mycokeys.117.145587","url":null,"abstract":"<p><p>Yunnan Province is rich in freshwater resources, providing a favorable environment for freshwater fungi. As a result, numerous new species have been described in recent years, further supporting the extensive diversity of freshwater fungi in this region. During an investigation of lignicolous freshwater fungi from the Dulongjiang River, six fresh isolates of Dictyosporiaceae were obtained. The new collection includes two new species (<i>Dictyocheirosporasplendida</i> and <i>Jalapriyaguttulata</i>), two new records (<i>D.nabanheensis</i> and <i>Dictyosporiumhughesii</i>), and one known species (<i>Dictyosporiumduliujiangense</i>). They are introduced based on morphological evidence and molecular phylogenetic analysis of combined ITS, LSU, and <i>tef</i>1-α sequence data. Detailed descriptions and illustrations of these species are provided, and morphological comparison with similar taxa is discussed.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"1-27"},"PeriodicalIF":2.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144046648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Two new species of <i>Penicillium</i> and a new genus in <i>Xylariomycetidae</i> from the forest dump-sites in Chiang Mai, Thailand.","authors":"Tanapol Thitla, Jutamart Monkai, Weiqian Meng, Surapong Khuna, Ning Xie, Sinang Hongsanan, Saisamorn Lumyong","doi":"10.3897/mycokeys.116.150635","DOIUrl":"https://doi.org/10.3897/mycokeys.116.150635","url":null,"abstract":"<p><p>Waste accumulation in forest regions can have a severe impact on the soil mycobiome. However, research on soil fungi inhabiting forest disposal sites remains limited. Therefore, this study focused on the taxonomy and phylogeny of ascomycetes isolated from soil in forest dump-sites in Chiang Mai, Thailand. The fungal strains were identified using morphological characterisations and multigene phylogenetic reconstruction. A new genus, <i>Pseudoleptodontidium</i>, typified by <i>Ps.chiangmaiense</i> <b>sp. nov.</b> (Amphisphaeriales genera <i>incertae sedis</i>, Xylariomycetidae), along with two new species, <i>Penicilliumchiangmaiense</i> (series <i>Janthinella</i>, section Lanata-Divaricata) and <i>P.terrae</i> (series <i>Erubescentia</i>, section Exilicaulis) (Aspergillaceae, Eurotiales), are described in detail and compared with closely-related species. Our discovery offers valuable insights into the soil ascomycetes associated with forest disturbances.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"116 ","pages":"275-301"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12056519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycokeysPub Date : 2025-04-29eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.116.146683
Hai-Yan Wang, Chunbo Dong, Yan-Wei Zhang, Wan-Hao Chen, Yan-Feng Han
{"title":"<i>Biconidiumsinense</i> gen. et sp. nov. (Hypocreales, Bionectriaceae) and <i>Didymocyrtisshanxiensis</i> sp. nov. (Phaeosphaeriaceae, <i>Didymocyrtis</i>) isolated from urban soil in China.","authors":"Hai-Yan Wang, Chunbo Dong, Yan-Wei Zhang, Wan-Hao Chen, Yan-Feng Han","doi":"10.3897/mycokeys.116.146683","DOIUrl":"https://doi.org/10.3897/mycokeys.116.146683","url":null,"abstract":"<p><p>During a fungal diversity survey in various urban habitats across China, 5 fungal isolates were discovered from soil samples. Detailed morphological observations and multi-gene phylogenetic analyses confirmed the identification of two novel taxa: <i>Biconidiumsinense</i> <b>gen. et sp. nov.</b> and <i>Didymocyrtisshanxiensis</i> <b>sp. nov.</b> These species were formally described, illustrated, and discussed, highlighting their distinct characteristics and taxonomic placement. The study expands our understanding of fungal diversity in urban environments, emphasizing the importance of combining morphological and molecular approaches for accurate species delineation and discovery.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"116 ","pages":"327-344"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12056516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycokeysPub Date : 2025-04-29eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.116.145568
Lin-Gen Chen, Hong Chen, Ling Ding, Yu-Qin Xu, Hui Zeng, Sheng-Nan Wang, Jun-Qing Yan
{"title":"Four new species of <i>Entoloma</i> subgenus <i>Cyanula</i> (Entolomataceae, Agaricales) from subtropical regions of China.","authors":"Lin-Gen Chen, Hong Chen, Ling Ding, Yu-Qin Xu, Hui Zeng, Sheng-Nan Wang, Jun-Qing Yan","doi":"10.3897/mycokeys.116.145568","DOIUrl":"https://doi.org/10.3897/mycokeys.116.145568","url":null,"abstract":"<p><p>In this study, four species of EntolomasubgenusCyanula (<i>E.orientosinense</i>, <i>E.subgriseosquamulosum</i>, <i>E.subpraegracile</i>, and <i>E.wuyishanense</i>) from subtropical regions of China, are described as new to science based on morphological and phylogenetic analyses. Morphologically, <i>E.orientosinense</i> is characterized by the white basidiomata, relatively large basidiospores, and carneogriseum-type lamellae edge; <i>E.subgriseosquamulosum</i> is recognized by the fuscous pileus, crowded and adnate lamellae, and medium-sized basidiospores; <i>E.subpraegracile</i> is identified by the yellow pileus and intervenose lamellae with sterile or heterogeneous edge; <i>E.wuyishanense</i> is distinct by the blue basidiomata and fertile lamellae edge with slightly bluish pigmentation near the stipe. <i>Entolomaorientosinense</i> belongs to sect. Caesiocincta, subsect. Queletia, and <i>E.wuyishanense</i> belongs to sect. Poliopodes. The remaining two species each form independent branches and do not belong to any known sections. Detailed descriptions, color photos, and a key to related species are presented.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"116 ","pages":"303-325"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12056515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycokeysPub Date : 2025-04-25eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.116.149376
Rui-Na Liang, Xiang-Hao Lin, Miao-Miao An, Guo-Zhu Zhao
{"title":"Two new species of <i>Penicillium</i> (Eurotiales, Aspergillaceae) from China based on morphological and molecular analyses.","authors":"Rui-Na Liang, Xiang-Hao Lin, Miao-Miao An, Guo-Zhu Zhao","doi":"10.3897/mycokeys.116.149376","DOIUrl":"https://doi.org/10.3897/mycokeys.116.149376","url":null,"abstract":"<p><p><i>Penicillium</i> is a large and significant genus of fungi, exhibiting widespread distribution across diverse substrates. Ongoing taxonomic and nomenclatural revisions have led to an annual increase in the number of newly described species. This study described two new <i>Penicillium</i> species, i.e., <i>P.lentum</i> and <i>P.tibetense</i>, discovered in China. They have been identified and characterized through morphological examination and both single gene and multigene phylogenetic analyses. Based on these analyses, <i>P.lentum</i> was classified within the section Brevicompacta, while <i>P.tibetense</i> was placed in the section Lanata-Divaricata. Both species exhibited the morphological features typical of their respective sections. <i>Penicilliumlentum</i> is characterized by restricted growth with dense colonies on agar media and predominantly generates terverticillate conidiophores. <i>Penicilliumtibetense</i> demonstrates rapid growth on media and has vigorous growth on CYA at 30 °C, producing biverticillate conidiophores. Comprehensive descriptions and detailed illustrations of these new species were presented. A morphological comparison between the new species and their closely related taxa was provided.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"116 ","pages":"255-274"},"PeriodicalIF":2.8,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biotrophic and saprophytic fungi from the <i>Rhodocybe</i>-<i>Clitopilus</i> clade (Entolomataceae): two new species and one new record in subtropical China.","authors":"Sipeng Jian, Xia Chen, Tianwei Yang, Xinjing Xu, Feng Gao, Yiwei Fang, Jing Liu, Chunxia Zhang","doi":"10.3897/mycokeys.116.148775","DOIUrl":"https://doi.org/10.3897/mycokeys.116.148775","url":null,"abstract":"<p><p>This study proposes two new species and a new record in the <i>Rhodocybe-Clitopilus</i> clade, based on comprehensive morphological and molecular analyses. The nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS), the large subunit ribosomal RNA gene (LSU), the RNA polymerase II second largest subunit (<i>RPB2</i>) and the translation elongation factor 1-alpha gene (<i>TEF1</i>), were employed to elucidate the relationships of <i>Clitopilus</i> and <i>Rhodocybe</i>. The first species, <i>Clitopilusparasiticus</i>, is capable of infecting the leaves of host plants in the genera <i>Dryopteris</i> and <i>Oplismenus</i>, exhibiting typical biotrophic behaviour while also demonstrating saprophytic growth on soil. Intraspecific comparisons were conducted, examining environmental factors as well as macro- and microscopic characteristics amongst individuals found on different plant hosts. Furthermore, this study reports the new saprophytic species, <i>Rhodocybezijinshanensis</i> and provides a detailed description of <i>Clitopilusbaronii</i>, a newly-recorded species in China.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"116 ","pages":"227-254"},"PeriodicalIF":2.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12046341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Three new species of <i>Apiospora</i> (Apiosporaceae, Amphisphaeriales) associated with diseased bamboo in China.","authors":"Xiaoyun Chang, Yuanyuan Wang, Tao Xu, Guangshuo Li, Xianghua Yue, MingJun Chen","doi":"10.3897/mycokeys.116.142263","DOIUrl":"https://doi.org/10.3897/mycokeys.116.142263","url":null,"abstract":"<p><p><i>Apiospora</i> is widely distributed worldwide, primarily comprising pathogens, endophytes, and saprobes associated with plants, and most of its hosts are Poaceae. In this study, 37 pathogenic strains of <i>Apiospora</i> were isolated from diseased bamboo collected in the provinces of Hunan and Guizhou, China. Multilocus phylogenetic analysis using combined ITS, LSU, <i>TUB2</i>, and <i>TEF1</i> sequence data, along with morphological assessments, identified three new species: <i>A.bambusiparasitica</i> <b>sp. nov.</b>, <i>A.qiannanensis</i> <b>sp. nov.</b>, and <i>A.xiangxiense</i> <b>sp. nov.</b> Descriptions, illustrations, and phylogenetic trees for the newly discovered species are provided and compared with closely related <i>Apiospora</i> species to enhance our understanding of the genus <i>Apiospora</i>. The pathogenicity test results demonstrated that the three new species could cause bamboo culm diseases, providing valuable information for the diagnosis and management of bamboo culm diseases.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"116 ","pages":"205-226"},"PeriodicalIF":2.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycokeysPub Date : 2025-04-23eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.116.142750
Jieting Li, Yi Li, Jiangrong Li, Ning Jiang
{"title":"Species of <i>Diaporthe</i> (Diaporthaceae, Diaporthales) associated with <i>Alnusnepalensis</i> leaf spot and branch canker diseases in Xizang, China.","authors":"Jieting Li, Yi Li, Jiangrong Li, Ning Jiang","doi":"10.3897/mycokeys.116.142750","DOIUrl":"https://doi.org/10.3897/mycokeys.116.142750","url":null,"abstract":"<p><p><i>Alnusnepalensis</i> is an important tree species in the Himalayas with significant ecological and economic roles. During disease surveys in Xizang, China, we observed leaf spot and branch canker symptoms on this tree. Fungal isolates associated with these diseases were collected and identified based on morphological characteristics and phylogenetic analysis of ITS, <i>cal</i>, <i>his3</i>, <i>tef1</i>, and <i>tub2</i> sequences. As a result, <i>Diaporthealnicola</i> <b>sp. nov.</b> and <i>D.amygdali</i> were identified from the leaf spots, while <i>D.linzhiensis</i> was identified to be associated with the cankered branches. This study identifies pathogenic species from alder trees, providing a foundation for future disease management and forest health research.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"116 ","pages":"185-204"},"PeriodicalIF":2.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}