{"title":"Four novel endolichenic fungi from <i>Usnea</i> spp. (Lecanorales, Parmeliaceae) in Yunnan and Guizhou, China: Taxonomic description and preliminary assessment of bioactive potentials.","authors":"Runlei Chang, Zhaoqi Yan, Jibo Jiang, Yichen Wang, Hongli Si, Tanay Bose, Congcong Miao","doi":"10.3897/mycokeys.118.155248","DOIUrl":"10.3897/mycokeys.118.155248","url":null,"abstract":"<p><p><i>Usnea</i> is one of the largest and most diverse genera of fruticose lichens with global distribution. Endolichenic fungi, which thrive within lichen thalli, have emerged as a promising source of bioactive compounds, with the ability to synthesise a variety of metabolites with biopharmaceutical potential. In this study, four isolates of endolichenic fungi isolated from <i>Usnea</i> spp. were identified using comprehensive multi-gene phylogenetic analyses. These isolates were evaluated for their anticancer, antifungal, and antibacterial properties, as well as for their ability to produce extracellular enzymes. Our findings revealed that the isolates represent four novel species, named as <i>Amphisphaeriafalcata</i>, <i>Kirschsteiniotheliatumidula</i>, <i>Neoroussoellaannulata</i>, and <i>Veronaeabrunneicolor</i>. Our screening assay showed <i>N.annulata</i> and <i>V.brunneicolor</i> exhibited cytotoxic effects against the H460 human lung cancer cell line, with moderate inhibitory activity at a concentration of 100 μg/mL. The four fungal isolates exhibited distinct antifungal profiles against phytopathogens: <i>Amphisphaeriafalcata</i> specifically inhibited <i>Fusariumgraminearum</i>, while <i>Veronaeabrunneicolor</i> showed broad-spectrum activity against <i>Botrytiscinerea</i>, <i>F.graminearum</i>, and <i>Alternariaalternata</i>. No antibacterial effects were detected in any isolates. These fungi exhibited a diverse array of extracellular enzyme activities, including amylase, protease, gelatinase, glucose oxidase, and cellulase. Collectively, these results underscore the considerable biotechnological potential of endolichenic fungi as sources of bioactive compounds with applications in drug discovery, agriculture, and environmental management. These findings also highlight the ecological importance of endolichenic fungi, suggesting that they may play multifaceted roles in lichen symbioses and their environments. Continued exploration of these fungi is essential for unlocking their full pharmacological and industrial potential.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"118 ","pages":"55-80"},"PeriodicalIF":2.8,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144267724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphological and phylogenetic analysis reveal three new species <i>Phyllosticta</i> (Phyllostictaceae, Botryosphaeriales) in China.","authors":"Meng-Yuan Zhang, Zhao-Xue Zhang, Du-Hua Li, Xiu-Guo Zhang, Ji-Wen Xia, Zhuang Li","doi":"10.3897/mycokeys.118.153609","DOIUrl":"10.3897/mycokeys.118.153609","url":null,"abstract":"<p><p><i>Phyllosticta</i> (Phyllostictaceae, Botryosphaeriales) species have been reported worldwide and collected from various plant hosts. We proposed three new species, viz., <i>Phyllostictaelliptica</i> <b>sp. nov.</b>, <i>P.rhododendri</i> <b>sp. nov.</b>, and <i>P.wuzhishanensis</i> <b>sp. nov.</b>, based on multi-locus phylogenetic analysis using a combined dataset of ITS rDNA, LSU, <i>tef1</i>, ACT, and GPDH, along with morphological characteristics. Moreover, <i>P.capitalensis</i>, isolated from the leaves of <i>Mangiferaindica</i>, is redescribed herein. We have re-examined the six species complexes: the <i>P.capitalensis</i> species complex (including 32 species), <i>P.concentrica</i> species complex (including 32 species), <i>P.cruenta</i> species complex (including 22 species), <i>P.owaniana</i> species complex (including six species), <i>P.rhodorae</i> species complex (including two species), and <i>P.vaccinii</i> species complex (including two species). Detailed descriptions and illustrations of the new species are provided. These findings enrich the biodiversity of fungi and provide reference for subsequent research.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"118 ","pages":"35-54"},"PeriodicalIF":2.8,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycokeysPub Date : 2025-05-22eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.118.143277
Jia-Jun Hu, Yong-Lan Tuo, Zheng-Xiang Qi, Dong-Hua Jiang, Yu Li, Bo Zhang
{"title":"Two new species of <i>Gymnopus</i> sect. <i>Levipedes</i> (Omphalotaceae, Agaricales) from Central and North China.","authors":"Jia-Jun Hu, Yong-Lan Tuo, Zheng-Xiang Qi, Dong-Hua Jiang, Yu Li, Bo Zhang","doi":"10.3897/mycokeys.118.143277","DOIUrl":"10.3897/mycokeys.118.143277","url":null,"abstract":"<p><p>The genus <i>Gymnopus</i> has a long research history and is known for its high species diversity worldwide. However, its species diversity in China remains poorly understood. Through a combination of detailed morphological studies and phylogenetic analysis, this study described two new species from Northeast and Central China, <i>Gymnopusbiyangensis</i> and <i>Gymnopussinodryophilus</i>, both belonging to Gymnopussect.Levipedes. <i>Gymnopusbiyangensis</i> is characterized by basidiomata appearing in summer and originating from broad-leaved forests, a dark reddish-brown pileus, a cylindrical to clavate stipe, and clavate to cylindrical, diverticulate cheilocystidia. <i>Gymnopussinodryophilus</i> differs by the yellowish-white to light brown basidiomata arising from coniferous and broad-leaved mixed forests, coralloid but inflated pileipellis terminal cells, and an apex of cheilocystidia not coralloid. Additionally, a key to the reported species of Gymnopussect.Levipedes in China is provided.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"118 ","pages":"19-34"},"PeriodicalIF":2.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycokeysPub Date : 2025-05-19eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.118.151514
Xiao-Yan Ma, Yong-Zhong Lu, Lei He, Dan-Dan Song, Jian Ma
{"title":"Two new species of <i>Neohelicosporium</i> (Tubeufiaceae, Tubeufiales) from freshwater and terrestrial habitats in China.","authors":"Xiao-Yan Ma, Yong-Zhong Lu, Lei He, Dan-Dan Song, Jian Ma","doi":"10.3897/mycokeys.118.151514","DOIUrl":"10.3897/mycokeys.118.151514","url":null,"abstract":"<p><p><i>Neohelicosporium</i> species are a group of helicosporous hyphomycetes with the potential to produce novel bioactive natural compounds. During our investigation on helicosporous hyphomycetes, six isolates were collected from freshwater and terrestrial habitats in the tropical and subtropical regions of southern China. Based on multi-gene phylogenetic analyses (LSU-ITS-<i>tef</i>1-α-<i>rpb</i>2) and morphological comparisons of <i>Neohelicosporium</i> taxa, two new species (<i>N.guizhouense</i> and <i>N.wuzhishanense</i>) are introduced. Detailed micro-morphological descriptions, illustrations, and DNA molecular data are provided for the newly introduced species to confirm their taxonomic placements.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"118 ","pages":"1-17"},"PeriodicalIF":2.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12107276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycokeysPub Date : 2025-05-15eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.117.150376
Hui Chen, Shabana Bibi, Ling Tao, Xiangchun Shen, Jun Zhao, Yueming Sun, Qirui Li, Dexiang Tang, Yao Wang
{"title":"<i>Papiliomycessinensis</i> (Clavicipitaceae) and <i>Paraisariapseudoarcta</i> (Ophiocordycipitaceae), two new species parasitizing Lepidopteran insects from southwestern China.","authors":"Hui Chen, Shabana Bibi, Ling Tao, Xiangchun Shen, Jun Zhao, Yueming Sun, Qirui Li, Dexiang Tang, Yao Wang","doi":"10.3897/mycokeys.117.150376","DOIUrl":"10.3897/mycokeys.117.150376","url":null,"abstract":"<p><p><i>Cordyceps</i> <i>sensu lato</i> species are highly important for medicinal purposes and functional food nutrients. Two new species belonging to <i>Cordyceps</i> <i>sensu lato</i> are introduced, i.e., <i>Papiliomycessinensis</i> and <i>Paraisariapseudoarcta</i>. To comprehensively describe the significance of these two species, morphological data were supplemented with phylogenetic analyses based on six loci (nrSSU, ITS, nrLSU, <i>tef-1α</i>, <i>rpb1</i>, and <i>rpb2</i>). Phylogenetically, <i>Pap.sinensis</i> is most closely related to <i>Pap.albostromaticus</i> and <i>Pap.shibinensis</i>, yet it can be distinguished from them by its larger stromata (51.3-85.7 × 3.1-3.5 vs. 37.0-58.0 × 2.5-3.0) and longer phialides (10.1-26.9 × 0.9-3.3 vs. 9.8-24.3 × 1.5-3.1). <i>Paraisariapseudoarcta</i> is phylogenetically sister to <i>Par.arcta</i>. The longer stromata (43-51 vs. 16) and larger secondary ascospores (5.6-8.3 × 1.7-3.1 vs. 2.6-4.2 × 0.5-1.3) in <i>Par.pseudoarcta</i> are characteristics that distinguish the two species. A thorough morphological description and phylogenetic analysis of <i>Pap.sinensis</i> and <i>Par.pseudoarcta</i> were provided. In addition, taxonomic misconceptions of <i>Par.gracilis</i> (Ophiocordycipitaceae) were corrected.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"353-374"},"PeriodicalIF":2.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phylogenetic evidence reveal a close relationship between <i>Amphichorda</i> and <i>Ovicillium</i> in Bionectriaceae (Hypocreales).","authors":"Yao Wang, De-Xiang Tang, Hui Chen, Qi-Rui Li, Chanhom Loinheuang, Xiang-Chun Shen","doi":"10.3897/mycokeys.117.151366","DOIUrl":"10.3897/mycokeys.117.151366","url":null,"abstract":"<p><p>Animal excrement serves as the primary substrate for <i>Amphichorda</i>, which is found in a wide range of habitats. Based on evolutionary relationships, the genus is currently classified within the Bionectriaceae. However, the phylogenetic position of <i>Amphichorda</i> and its associated taxa remains unresolved due to limited sampling in previous studies. Here, we discovered and identified five <i>Amphichorda</i> species, significantly advancing our understanding of this genus. Using six genomic loci (ITS, nrSSU, nrLSU, <i>tef1α</i>, <i>rpb1</i>, and <i>rpb2</i>) to expand taxonomic sampling, we reconstructed a phylogenetic framework for the Bionectriaceae, with a focus on <i>Amphichorda</i> and related taxa. Phylogenetic analyses revealed a close genetic connection between <i>Amphichorda</i> and related genera, yet they formed distinct clades within the Bionectriaceae and were clearly differentiated. The extensive sampling demonstrated stable phylogenetic relationships among <i>Amphichorda</i>, <i>Hapsidospora</i>, <i>Ovicillium</i>, <i>Proxiovicillium</i>, and <i>Bulbithecium</i>. Furthermore, we described two new species, <i>A.guizhouensis</i> <b>sp. nov.</b> and <i>O.pseudoattenuatum</i> <b>sp. nov.</b>, supported by DNA data and morphological characteristics. A comprehensive comparison of morphological traits across all members of <i>Amphichorda</i> and <i>Ovicillium</i> was conducted. This study clarifies taxonomic boundaries and evolutionary relationships within the two genera and contributes to the overall understanding of the biodiversity and systematics of the Bionectriaceae.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"337-352"},"PeriodicalIF":2.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12099312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycokeysPub Date : 2025-05-14eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.117.154573
Mei-Yan Han, Samantha C Karunarathna, Li Lu, De-Ge Zheng, Nakarin Suwannarach, Abdallah M Elgorban, Dong-Qin Dai, Li-Juan Zhang, Wan-Tong Zhao, Ekachai Chukeatirote, Saowaluck Tibpromma
{"title":"Three new species of <i>Camporesiomyces</i> (Tubeufiaceae, Tubeufiales, Dothideomycetes) associated with coffee in Yunnan, China.","authors":"Mei-Yan Han, Samantha C Karunarathna, Li Lu, De-Ge Zheng, Nakarin Suwannarach, Abdallah M Elgorban, Dong-Qin Dai, Li-Juan Zhang, Wan-Tong Zhao, Ekachai Chukeatirote, Saowaluck Tibpromma","doi":"10.3897/mycokeys.117.154573","DOIUrl":"10.3897/mycokeys.117.154573","url":null,"abstract":"<p><p>During our surveys of microfungi associated with coffee plants in Yunnan Province, China, three saprobic fungi were isolated from dead coffee branches. Multigene phylogenetic analyses (ITS, LSU, <i>tef</i>1-α, and <i>rpb</i>2) and morphological characteristics resulted in the identification of three novel species in <i>Camporesiomyces</i>, namely <i>C.bhatii</i>, <i>C.coffeae</i>, and <i>C.puerensis</i>. Detailed morphological descriptions, illustrations, and phylogenetic analyses of these three new species are provided, along with morphological comparisons to closely related taxa. These findings have global implications for understanding the diversity of microfungi associated with coffee trees.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"315-336"},"PeriodicalIF":2.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144129329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycokeysPub Date : 2025-05-13eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.117.144875
Yu Yang, Yuan-Pin Xiao, Ruvishika S Jayawardena, Kevin D Hyde, Somrudee Nilthong, Ausana Mapook, Yong-Zhong Lu, Shu-Qiong Xie, Fatimah Al-Otibi, Xiao Wang, Kang Luo, Li-Ping Luo
{"title":"Three new species of <i>Ophiocordyceps</i> (Hypocreales, Ophiocordycipitaceae) and a new host record for <i>Hirsutellavermicola</i> from China.","authors":"Yu Yang, Yuan-Pin Xiao, Ruvishika S Jayawardena, Kevin D Hyde, Somrudee Nilthong, Ausana Mapook, Yong-Zhong Lu, Shu-Qiong Xie, Fatimah Al-Otibi, Xiao Wang, Kang Luo, Li-Ping Luo","doi":"10.3897/mycokeys.117.144875","DOIUrl":"10.3897/mycokeys.117.144875","url":null,"abstract":"<p><p><i>Ophiocordyceps</i>, a genus of invertebrate-pathogenic fungi in the family Ophiocordycipitaceae (order Hypocreales), is globally distributed. Over 300 species have been described and accepted as members of the genus. This study introduces three new <i>Ophiocordyceps</i> species from China: <i>O.liaoningensis</i>, <i>O.muscidarum</i>, and <i>O.neocommunis</i>. Remarkably, <i>O.muscidarum</i>, hosted on flies (Muscidae, Diptera), is characterized by its larger perithecia and longer secondary ascospores. <i>Ophiocordycepsneocommunis</i> is also introduced based on morphological distinctions from closely related species. <i>Ophiocordycepsliaoningensis</i> produces dark brown superficial perithecia with an asexual morph at the apex and does not break into part-spores. Phylogenetic analyses using six loci (LSU, ITS, SSU, <i>tef-1α</i>, <i>rpb1</i>, and <i>rpb2</i>) robustly support the placement of these new species within the <i>Ophiocordyceps</i>. Additionally, we report a new host record for <i>Hirsutellavermicola</i>. Detailed descriptions, illustrations, color photo plates, and a phylogenetic tree are provided.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"289-313"},"PeriodicalIF":2.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12093112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycokeysPub Date : 2025-05-09eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.117.149185
Xin-Yu Ji, Zi-Ying Ding, Yong Nie, Heng Zhao, Shi Wang, Bo Huang, Xiao-Yong Liu
{"title":"Unveiling species diversity within early-diverging fungi from China V: Five new species of <i>Absidia</i> (Cunninghamellaceae, Mucoromycota).","authors":"Xin-Yu Ji, Zi-Ying Ding, Yong Nie, Heng Zhao, Shi Wang, Bo Huang, Xiao-Yong Liu","doi":"10.3897/mycokeys.117.149185","DOIUrl":"10.3897/mycokeys.117.149185","url":null,"abstract":"<p><p><i>Absidia</i> is widely distributed in soil, koji, and various types of feces. A multi-locus phylogeny covering the small subunit (SSU), internal transcribed spacer (ITS), and large subunit of ribosomal RNA gene (LSU rDNA), translation elongation factor 1-alpha (<i>TEF1α</i>), and actin (<i>Act</i>), combined with morphological characteristics, revealed five new species in this genus. This study provides their descriptions and illustrations and discusses their differences from morphological allies and phylogenetic relatives. <i>Absidiacollariata</i> <b>sp. nov.</b> is distinguished from other species in terms of the length of collars. <i>A.hainanensis</i> <b>sp. nov.</b> is named after the geographical location Hainan, distinctive with a higher maximum growing temperature. <i>A.pyriformis</i> <b>sp. nov.</b> is different from other species in terms of sporangial shape. <i>A.tardiva</i> <b>sp. nov.</b> is characterized by slow growth. And <i>A.tibetensis</i> <b>sp. nov.</b> is named after the geographical location Tibet and differentiated by more ampulliform swellings. This study further enriches the species diversity of <i>Absidia</i> as the latest discovery of early-diverging fungi in China.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"267-288"},"PeriodicalIF":2.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12084811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144095317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycokeysPub Date : 2025-05-09eCollection Date: 2025-01-01DOI: 10.3897/mycokeys.117.137112
Maryam Fallahi, Alireza Armand, Fatimah Al-Otibi, Kevin D Hyde, Ruvishika S Jayawardena
{"title":"Pathogenic fungi (Sordariomycetes) associated with annual and perennial crops in Northern Thailand.","authors":"Maryam Fallahi, Alireza Armand, Fatimah Al-Otibi, Kevin D Hyde, Ruvishika S Jayawardena","doi":"10.3897/mycokeys.117.137112","DOIUrl":"10.3897/mycokeys.117.137112","url":null,"abstract":"<p><p>Phytopathogenic fungi induce severe diseases in plant hosts, potentially leading to significant crop quantity and quality reductions. In this study, we isolated and identified pathogenic fungi that threaten the successful cultivation of annual and perennial crops in northern Thailand. Samples with leaf spots, fruit rot, wilting, and dieback symptoms were collected from 19 different crops in Chiang Rai, Chiang Mai, and Sakon Nakhon provinces in Thailand. Out of 183 isolates, 27 were selected for molecular analysis and multi-gene phylogenetic study based on their diverse host range, geographical distribution, and an initial morphological assessment. Four of the most species-diverse fungal genera - <i>Colletotrichum</i>, <i>Diaporthe</i>, <i>Fusarium</i>, and <i>Neopestalotiopsis</i> - were identified, along with 23 taxa, including four novel species. Six species of <i>Colletotrichum</i> (<i>Colletotrichumabelmoschi</i> <b>sp. nov.</b>, <i>C.fructicola</i>, <i>C.makassarense</i>, <i>C.plurivorum</i>, <i>C.siamense</i>, and <i>C.spaethianum</i>), six species of <i>Diaporthe</i> (<i>Diaporthefistulosi</i> <b>sp. nov.</b>, <i>D.hongkongensis</i>, <i>D.melongenicola</i> <b>sp. nov.</b>, <i>D.rosae</i>, <i>D.sennicola</i>, and <i>D.siamensis</i>), five species of <i>Fusarium</i> (<i>Fusariumbubalinum</i>, <i>F.languescens</i>, <i>F.nirenbergiae</i>, <i>F.sulawesiense</i>, and <i>F.tanahbumbuense</i>), and three species of <i>Neopestalotiopsis</i> (<i>Neopestalotiopsisformicidarum</i>, <i>N.theobromicola</i> <b>sp. nov.</b>, and <i>N.zakeelii</i>, along with three unspecified taxa), were isolated and characterized in this study. Additionally, fourteen new host records and eight new geographical records are reported. Pathogenicity tests were carried out for selected strains, and the results confirmed their pathogenicity on their host plants. This study offers new insights into the diversity of species of plant pathogenic fungi in northern Thailand.</p>","PeriodicalId":48720,"journal":{"name":"Mycokeys","volume":"117 ","pages":"191-265"},"PeriodicalIF":2.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12084813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144095315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}