SoilPub Date : 2026-10-16DOI: 10.5194/soil-12-979-2026
Jennifer Alvarez-Sagrero,Asmeret Asefaw Berhe,Stephany S. Chacon,Jeffrey P. Mitchell,Teamrat Afewerki Ghezzehei
{"title":"Two decades of conservation agriculture enhances soil structure, carbon sequestration, and water retention in Mediterranean soils","authors":"Jennifer Alvarez-Sagrero,Asmeret Asefaw Berhe,Stephany S. Chacon,Jeffrey P. Mitchell,Teamrat Afewerki Ghezzehei","doi":"10.5194/soil-12-979-2026","DOIUrl":"https://doi.org/10.5194/soil-12-979-2026","url":null,"abstract":"Conservation agriculture offers a pathway for enhancing soil health with climate co-benefits in Mediterranean agricultural systems. This study examined long-term impacts of combining reduced tillage with cover cropping over 20 years in California's Central Valley, providing insights into soil system response under sustained conservation management. We assessed soil physical, chemical, and structural properties comparing reduced tillage with cover crops (CTCC) to standard tillage without cover crops (STNC), employing density fractionation and spectroscopic analysis to understand carbon protection mechanisms. After two decades, conservation agriculture produced soil conditions consistent with the approach toward a management-induced steady state described for systems managed for 15–20 years (Caruso et al., 2018; West and Post, 2002), characterized by shifts in carbon stabilization pathways. Water-stable aggregate analysis revealed the most pronounced management effects, with CTCC exhibiting 136 % greater stability than STNC, indicating substantial improvements in soil structural integrity. These structural enhancements corresponded with a reorganization of carbon protection mechanisms: CTCC disproportionately enriched the occluded light fraction (44.1 % vs. 35.4 % of total recovered carbon in STNC), indicating that aggregate-mediated physical protection becomes a dominant carbon stabilization pathway under long-term conservation management. Mineral-associated organic carbon saturation analysis revealed that both management systems remained well below theoretical maximum capacity (11.5 % vs. 7.4 % saturation for CTCC and STNC, respectively), indicating that mineral surface availability is not the primary constraint on carbon storage in this Mediterranean system. Physical property improvements under CTCC included 15 % lower bulk density and 13 % greater water retention at field capacity, though benefits were concentrated in the surface horizon. Our findings demonstrate that two decades of conservation agriculture substantially altered soil functioning through aggregate-mediated physical protection, while creating measurable improvements in soil structural integrity and water retention capacity. These mechanism shifts are consistent with a soil system that maintains enhanced functionality and continued carbon sequestration potential in Mediterranean agricultural systems.","PeriodicalId":48610,"journal":{"name":"Soil","volume":"12 1","pages":"979-998"},"PeriodicalIF":6.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SoilPub Date : 2026-10-13DOI: 10.5194/soil-12-963-2026
Xiaoshuai Song, Chenyang Xu, Huanhuan Zhao, Jiangwen Li, Wei Du, Wuquan Ding, Zhenghong Yu, Fengbao Zhang, Feinan Hu
{"title":"Biochar addition enhances soil aggregate stability by modulating soil internal forces: the critical role of readily oxidized organic carbon and particulate organic carbon","authors":"Xiaoshuai Song, Chenyang Xu, Huanhuan Zhao, Jiangwen Li, Wei Du, Wuquan Ding, Zhenghong Yu, Fengbao Zhang, Feinan Hu","doi":"10.5194/soil-12-963-2026","DOIUrl":"https://doi.org/10.5194/soil-12-963-2026","url":null,"abstract":"Abstract. Degradation of soil structure and function is a significant obstacle to sustainable agriculture, yet using biochar is known to be an effective way of increasing soil organic carbon and boosting structure. Aggregate stability is greatly influenced by soil internal forces (SIFs), such as van der Waals, hydration, and electrostatic forces. However, it is still not clear which specific organic carbon components play a significant role in the stability of aggregates and the action of soil internal forces. In this study, a field simulation experiment was conducted using maize straw and wheat straw biochars to investigate how biochar addition affects soil aggregate stability. The findings showed that as the rate of biochar application increased from 0 % to 10 % over 180 and 365 d, the contents of soil light fraction organic carbon (LFOC), particulate organic carbon (POC), dissolved organic carbon (DOC), readily oxidized organic carbon (ROC), as well as soil specific surface area and surface charge number, increased. The content of different carbon fractions decreased with prolonged incubation time, while soil electrochemical parameters continued to increase throughout the incubation period. Pearson correlation and redundancy analyses showed that soil active organic carbon fractions were strongly correlated with soil surface electrochemical properties, with ROC and POC being the primary factors. Soil aggregate stability increased with higher biochar application rates, and the beneficial effect of biochar on aggregate stability became increasingly pronounced over time. Based on the quantitative estimates of SIFs, the addition of biochar facilitated soil aggregate stability by increasing van der Waals attractive pressure and decreasing electrostatic repulsive pressure, and finally lowering net repulsive pressure between soil particles. The soil aggregate breaking strength increased rapidly initially and then leveled out as the electrolyte content decreased; 10−2 mol L−1 marked the tipping point. The study identified the active organic carbon (ROC and POC) fractions which could mediate the soil surface electrochemical properties and soil internal forces, thereby enhancing soil aggregate stability. Our research provides a theoretical basis for the targeted use and modification of biochar to efficiently improve soil structure.","PeriodicalId":48610,"journal":{"name":"Soil","volume":"16 1","pages":""},"PeriodicalIF":6.8,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149152201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SoilPub Date : 2026-10-09DOI: 10.5194/egusphere-2026-5762
Ana Paula Ferreira de Brito, Mark Farrell, Luke Mosley
{"title":"Near-tree soil footprints show higher organic carbon and lower bulk density with no detectable end-of-summer moisture penalty","authors":"Ana Paula Ferreira de Brito, Mark Farrell, Luke Mosley","doi":"10.5194/egusphere-2026-5762","DOIUrl":"https://doi.org/10.5194/egusphere-2026-5762","url":null,"abstract":"<strong>Abstract.</strong> Scattered trees can create fine-scale surface soil enrichment, but it is unclear how far these patterns extend through the mineral soil profile, translate into carbon stocks, and influence moisture content. We quantified the soil functional footprint of 14 established trees from four contrasting cohorts in an arboretum–grassland system under a Mediterranean climate in South Australia. Soil was sampled at 1, 2, 4 and 8 m from each stem at depth intervals to 30 cm. Directly measured surface bulk density was lower at 1, 2 and 4 m than at 8 m, while soil organic carbon (SOC) concentration was 30% higher at 1 m and declined by 8% with each doubling of distance. Total nitrogen, nitrate-N and Colwell-extractable P also varied with distance, whereas gravimetric moisture did not decline towards the trees under the end-of-summer conditions sampled. Distance × tree cohort interactions were not significant for these principal responses. After accounting for depth and tree identity, distance explained 3% of joint soil property variation whereas depth explained 39%. On an equivalent-soil-mass basis, SOC stock at 1 m distance from the tree exceeded that at 8 m by 16.5 Mg C ha⁻¹, although the categorical fixed-depth 0–30 cm SOC-stock test was not significant. Within the radially-weighted 0-8 m footprint, we combined two carbon pools, model-assisted mineral-soil SOC and current projected whole-tree carbon. Together these amounted to 67.4–123.2 Mg C ha⁻¹, with mineral-soil SOC accounting for 59–89% of this combined soil tree total. These results identify a shared, property-specific radial soil footprint characterised by lower bulk density and higher carbon and nutrient concentrations without a detectable dry-season moisture penalty. They also demonstrate why depth resolution, soil mass correction and radial area weighting are essential when converting near-tree enrichment into spatially explicit carbon accounting and monitoring.<strong> </strong>Because the near-tree zone retained higher carbon and nutrients without an end-of-summer moisture penalty, established trees may support understorey forage production and its resilience under increasing aridity rather than imposing a dry-season water cost.","PeriodicalId":48610,"journal":{"name":"Soil","volume":"32 1","pages":""},"PeriodicalIF":6.8,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149130436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SoilPub Date : 2026-10-01DOI: 10.5194/soil-12-947-2026
Imane Achkouk, Anass El Yemlahi, Ouiam El Galiou, Amin Laglaoui, Mounir Hassani Zerrouk, Mohammed Bakkali, Abdelhay Arakrak
{"title":"Unraveling the plant growth promotion potential of Pseudomonas species isolated from the rhizosphere of Lotus creticus grown in the Mediterranean coastal regions of Morocco","authors":"Imane Achkouk, Anass El Yemlahi, Ouiam El Galiou, Amin Laglaoui, Mounir Hassani Zerrouk, Mohammed Bakkali, Abdelhay Arakrak","doi":"10.5194/soil-12-947-2026","DOIUrl":"https://doi.org/10.5194/soil-12-947-2026","url":null,"abstract":"Abstract. Rhizosphere-associated bacteria play a key role in enhancing plant performance under saline and nutrient-limited soil conditions, where plant establishment is strongly constrained. In this study, we evaluated the plant growth-promoting potential of rhizobacteria isolated from the rhizosphere of wild-growing Lotus creticus along the Mediterranean coast of Morocco. Of the 30 isolates, five bacterial strains (R125, P79, R8, R150, and R15) were selected based on their plant growth-promoting traits. These strains were identified through 16S rRNA gene sequencing as Pseudomonas protegens, Pseudomonas sesami, Pseudomonas versuta, Pseudomonas helleri, and Pseudomonas trivialis. Phenotypic characteristics, including IAA production, phosphate solubilization capacity, cellulase and protease activities, and tolerance to salinity and temperature, were evaluated. Additionally, a pot experiment was conducted to assess the impact of inoculation on L. creticus growth. Pseudomonas protegens P79 was characterized by strong IAA production, high phosphate solubilization capacity (150.5 mg L−1), and notable cellulase and protease activities. It also demonstrated high tolerance to salinity (up to 13 % NaCl) and temperature (up to 45 °C). Comparatively, Pseudomonas sesami R8 exhibited broad-spectrum antifungal activity, including strong inhibition of the growth of Aspergillus ochraceus. The pot experiment revealed that inoculation with Pseudomonas helleri R125 and Pseudomonas trivialis R150 significantly (P < 0.05) enhanced the aerial dry biomass of L. creticus by 300 %. In contrast, Pseudomonas protegens P79 was more effective in promoting root elongation by 8 % under growth chamber conditions. This study highlights the potential of Pseudomonas strains as promising biostimulants for sustainable agriculture and rehabilitation of coastal marginal soils under combined salinity and nutrient stresses.","PeriodicalId":48610,"journal":{"name":"Soil","volume":"272 1","pages":""},"PeriodicalIF":6.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149083849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SoilPub Date : 2026-09-23DOI: 10.5194/soil-12-915-2026
Teresa Alía, Sergio González-Ubierna, Abel Sánchez-Jiménez, Rubén Abad-Calderón, Miguel Ángel Casermeiro
{"title":"Soil moisture as the dominant driver of CO\u0000 2\u0000 efflux in Mediterranean urban green spaces: evidence for a Gaussian temperature response and mechanistic modelling of moisture and temperature interactions","authors":"Teresa Alía, Sergio González-Ubierna, Abel Sánchez-Jiménez, Rubén Abad-Calderón, Miguel Ángel Casermeiro","doi":"10.5194/soil-12-915-2026","DOIUrl":"https://doi.org/10.5194/soil-12-915-2026","url":null,"abstract":"Abstract. Soil respiration is a key component of the global carbon cycle, yet its dynamics in urban Mediterranean environments remain poorly understood. This study investigated the effects of soil temperature, soil moisture, and plant community on soil CO2 efflux in urban green spaces of Madrid (Spain), characterized by a Mediterranean pluvioseasonal-oceanic bioclimate with pronounced summer drought. Four dominant ruderal plant communities (Diplotaxis virgata, Hordeum leporinum, Malva spp., and Dactylis glomerata) were monitored across three urban green spaces. Soil CO2 efflux, temperature, and moisture were measured biweekly over one year using an infrared gas analyzer. Contrary to the Q10 exponential assumption, soil respiration showed a Gaussian relationship with temperature, with a positive correlation below a breakpoint of 18.4 °C and a negative effect above this threshold, consistent across plant communities. Soil respiration exhibited a positive exponential relationship with soil moisture and a logarithmic relationship with a rewetting index for values below 20. A mechanistic model described soil respiration as the joint outcome of temperature-driven moisture loss and moisture-stimulated CO2 emissions. Plant community identity had a limited effect, with the exception of Malva spp., which consistently produced higher emissions. These findings challenge the universal applicability of temperature-based respiration models and highlight soil moisture as the dominant driver of CO2 efflux in water-limited urban ecosystems. As climate change is expected to intensify both the urban heat island effect and summer drought in Mediterranean cities, soil moisture emerges as a critical variable for projecting urban soil CO2 fluxes and for designing evidence-based management strategies for Mediterranean urban green spaces, including improved soil infiltration capacity, moisture-sensitive irrigation planning, and the incorporation of moisture-temperature coupling into urban carbon monitoring protocols.","PeriodicalId":48610,"journal":{"name":"Soil","volume":"16 1","pages":"915-935"},"PeriodicalIF":6.8,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149021225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}