BMC Zoology最新文献

{"title":"Histamine and gamma-aminobutyric acid in the nervous system of Pygospio elegans (Annelida: Spionidae): structure and recovery during reparative regeneration.","authors":"Zinaida I Starunova,&nbsp;Ksenia V Shunkina,&nbsp;Elena L Novikova,&nbsp;Viktor V Starunov","doi":"10.1186/s40850-022-00160-7","DOIUrl":"https://doi.org/10.1186/s40850-022-00160-7","url":null,"abstract":"<p><strong>Background: </strong>In recent two decades, studies of the annelid nervous systems were revolutionized by modern cell labeling techniques and state-of-the-art microscopy techniques. However, there are still huge gaps in our knowledge on the organization and functioning of their nervous system. Most of the recent studies have focused on the distribution of serotonin and FMRFamide, while the data about many other basic neurotransmitters such as histamine (HA) and gamma-aminobutyric acid (GABA) are scarce.</p><p><strong>Results: </strong>Using immunohistochemistry and confocal microscopy we studied the distribution of histamine and gamma-aminobutyric acid in the nervous system of a spionid annelid Pygospio elegans and traced their redevelopment during reparative regeneration. Both neurotransmitters show specific patterns in central and peripheral nervous systems. HA-positive cells are concentrated mostly in the brain, while GABA-positive cell somata contribute equally to brain and segmental ganglia. Some immunoreactive elements were found in peripheral nerves. Both substances were revealed in high numbers in bipolar sensory cells in the palps. The first signs of regenerating HAergic and GABAergic systems were detected only by 3 days after the amputation. Further redevelopment of GABAergic system proceeds faster than that of HAergic one.</p><p><strong>Conclusions: </strong>Comparisons with other annelids and mollusks examined in this respect revealed a number of general similarities in distribution patterns of HAergic and GABAergic cells in different species. Overall, the differences in the full redevelopment of various neurotransmitters correlate with neuronal development during embryogenesis. Our results highlight the importance of investigating the distribution of different neurotransmitters in comparative morphological and developmental studies.</p>","PeriodicalId":48590,"journal":{"name":"BMC Zoology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9455184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth, maturity, reproduction, and life expectancy in ex-situ pacific walruses (Odobenus rosmarus divergens).","authors":"Todd Robeck,&nbsp;Etsuko Katsumata,&nbsp;Kazutoshi Arai,&nbsp;Gisele Montano,&nbsp;Todd Schmitt,&nbsp;Stacy DiRocco,&nbsp;Karen J Steinman","doi":"10.1186/s40850-022-00158-1","DOIUrl":"https://doi.org/10.1186/s40850-022-00158-1","url":null,"abstract":"<p><strong>Background: </strong>Pacific walruses are found in Arctic regions of the Chukchi and Bering Sea where rapid changes in environmental conditions resulting in loss of sea ice are occurring. Therefore, accurate life history data are crucial for species management plans and longitudinal data collected over the lives of individual walruses housed in zoos and aquaria provide otherwise difficult to obtain biological information.</p><p><strong>Results: </strong>While similar at birth, Gompertz regression curves indicated that males grew faster than females (p < 0.0001) in weight (99 kg vs 57.6 kg/y) and length (26.9 cm vs 26.3 cm/y) with physical differences being detected by age 3 for weight and age 7 for length. Males reached adult weight at 13.5 ± 3.3 y and females by age 12.3 ± 2.3 y. The mean age at first ovulation and at first conception occurred at 8.8 y and 9.6 y. Greater than 75% of all conceptions and calving occurred between February and March and from May to June, respectively. Mean gestation lasted 423 d and false pregnancies lasted at least 169 d with a decrease (p < 0.05) in serum progesterone concentration between false pregnancy and pregnancy occurring within 6 months after ovulation. Based on these results, we estimated embryonic diapause to last from 120 to 139 days, and fetal growth last ~ 284 days. All males older than 8 y had an increase in serum testosterone and body weight that was highest in February and lowest in July. Overall, no differences were observed between male and female survival, with a mean (± SEM) life expectancy of 19.5 ± 1.5 y, respectively. Currently, the oldest male and female captive walruses are 40 and 43 y, respectively.</p><p><strong>Conclusions: </strong>Data provided herein include details of life history characteristics of zoo and aquaria housed walruses that are useful for wild population recovery models. In particular, results on survivorship and the identification of the most vulnerable period for calf survival can help with model development and suggests that for recovery to occur birthing locations for this species must be protected.</p>","PeriodicalId":48590,"journal":{"name":"BMC Zoology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9453385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ontogenic gonadal transcriptomes provide insights into sex change in the ricefield eel Monopterus albus.","authors":"Miao Fan,&nbsp;Wei Yang,&nbsp;Weimin Zhang,&nbsp;Lihong Zhang","doi":"10.1186/s40850-022-00155-4","DOIUrl":"https://doi.org/10.1186/s40850-022-00155-4","url":null,"abstract":"<p><strong>Background: </strong>The ricefield eel is a freshwater protogynous hermaphrodite fish and has become an important aquaculture species in China. The sex change of ricefield eel is impeding its aquaculture practice, particularly the large-scale artificial breeding. Many studies including transcriptomes of mixed gonadal samples from different individuals have been aimed to elucidate mechanisms underlying the sex change. However, the key physiological factors involved in the initiation of sex change remain to be identified.  RESULTS: The present study performed transcriptomic analysis on gonadal samples of different sexual stages obtained through biopsy from the same fish undergoing sex change. A total of 539,764,816 high-quality reads were generated from twelve cDNA libraries of gonadal tissues at female (F), early intersexual (EI), mid-intersexual (MI), and late intersexual (LI) stages of three individual sex-changing fish. Pairwise comparisons between EI and F, MI and EI, and LI and MI identified 886, 319, and 10,767 differentially expressed genes (DEGs), respectively. Realtime quantitative PCR analysis of 12 representative DEGs showed similar expression profiles to those inferred from transcriptome data, suggesting the reliability of RNA-seq data for gene expression analysis. The expression of apoeb, csl2, and enpp2 was dramatically increased and peaked at EI while that of cyp19a1a, wnt4a, fgf16, and foxl2a significantly downregulated from F to EI and remained at very low levels during subsequent development until LI, which suggests that apoeb, csl2, enpp2, cyp19a1a, wnt4a, fgf16, and foxl2a may be closely associated with the initiation of sex change of ricefield eels.</p><p><strong>Conclusions: </strong>Collectively, results of the present study confirmed that the down-regulation of female-related genes, such as cyp19a1a, wnt4a, fgf16, and foxl2a, is important for the sex change of ricefield eels. More importantly, some novel genes, including apoeb, csl2, and enpp2, were shown to be expressed with peak values at EI, which are potentially involved in the initiation of sex change. The present transcriptomic data may provide an important research resource for further unraveling the mechanisms underlying the sex change and testicular development in ricefield eels as well as other teleosts.</p>","PeriodicalId":48590,"journal":{"name":"BMC Zoology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10300612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and morphometric studies on the axial skeleton of the sitatunga (Tragelaphus spekii gratus).","authors":"Kenechukwu Tobechukwu Onwuama,&nbsp;Chikera Samuel Ibe,&nbsp;Alhaji Zubair Jaji,&nbsp;Suleiman Olawoye Salami,&nbsp;Esther Solomon Kigir","doi":"10.1186/s40850-022-00157-2","DOIUrl":"https://doi.org/10.1186/s40850-022-00157-2","url":null,"abstract":"<p><strong>Background: </strong>Anatomical features of the skeleton of wild animals contribute largely to their adaptation. A dearth of information on the skeletal anatomy of the sitatunga (Tragelaphus spekii gratus) necessitated this study. Two adult sitatunga carcasses weighing 54 kg and 57 kg were obtained after post-mortem examination. Bone preparation was achieved through cold water maceration protocol.</p><p><strong>Result: </strong>The tympanic bulla was elongated and massive, resulting in the rudimentary appearance of the styloid and muscular processes of the temporal bone. The lacrimal bone had a somewhat triangular presentation with the lacrimal foramen on the caudal border of the facial surface while its dorsal border formed the lateral margin of the frontal sinus. There was no observable lacrimal fossa on this facial surface of the lacrimal bone. The facial tubercle was absent. The vertebral column formula was C7 T13 L6 S4 C10-14, and the atlas dorsal median tubercle was smooth, devoid of ridges. The spinous process of the axis extended the entire arch length to hang little above the odontoid process. The thoracic spinous processes were oriented dorso-caudally from T1 to T11; spinous process of T12 was vertical, while that of T13 was oriented dorso-cranially. The length of the transverse process of L1 and L6 were the same, and smaller than the length of those of L2-L5. There was incomplete fusion of sacral spinous processes. Three dorsal and ventral sacral foramina were identified laterally on each side of the vertebrae. The ribs were 26 in number (13 pairs). The sternum was comprised of 5 sternabrae separated by intersternal cartilage. The average number of bones of the axial skeleton was 75. Morphometric in formation included the length of skull, mandible and ribs; body length of vertebrae and spinous process length and height of the vertebrae.</p><p><strong>Conclusion: </strong>This study recorded anatomical features and biometric information on axial skeletal bones of the Sitatunga (Tragelaphus spekii gratus) thereby providing baseline data for future biomedical, archaeological and comparative skeletal anatomical studies.</p>","PeriodicalId":48590,"journal":{"name":"BMC Zoology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9447071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disarrangement and reorganization of the hippocampal functional connectivity during the spatial path adjustment of pigeons.","authors":"Mengmeng Li,&nbsp;Shuguan Cheng,&nbsp;Jiantao Fan,&nbsp;Zhigang Shang,&nbsp;Hong Wan,&nbsp;Lifang Yang,&nbsp;Long Yang","doi":"10.1186/s40850-022-00143-8","DOIUrl":"https://doi.org/10.1186/s40850-022-00143-8","url":null,"abstract":"<p><strong>Background: </strong>The hippocampus plays an important role to support path planning and adjustment in goal-directed spatial navigation. While we still only have limited knowledge about how do the hippocampal neural activities, especially the functional connectivity patterns, change during the spatial path adjustment. In this study, we measured the behavioural indicators and local field potentials of the pigeon (Columba livia, male and female) during a goal-directed navigational task with the detour paradigm, exploring the changing patterns of the hippocampal functional network connectivity of the bird during the spatial path learning and adjustment.</p><p><strong>Results: </strong>Our study demonstrates that the pigeons progressively learned to solve the path adjustment task after the preferred path is blocked suddenly. Behavioural results show that both the total duration and the path lengths pigeons completed the task during the phase of adjustment are significantly longer than those during the acquisition and recovery phases. Furthermore, neural results show that hippocampal functional connectivity selectively changed during path adjustment. Specifically, we identified depressed connectivity in lower bands (delta and theta) and elevated connectivity in higher bands (slow-gamma and fast-gamma).</p><p><strong>Conclusions: </strong>These results feature both the behavioural response and neural representation of the avian spatial cognitive learning process, suggesting that the functional disarrangement and reorganization of the connectivity in the avian hippocampus during different phases may contribute to our further understanding of the potential mechanism of path learning and adjustment.</p>","PeriodicalId":48590,"journal":{"name":"BMC Zoology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9453391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human-wildlife conflict and community perceptions towards wildlife conservation in and around Wof-Washa Natural State Forest, Ethiopia.","authors":"Dereje Yazezew","doi":"10.1186/s40850-022-00154-5","DOIUrl":"10.1186/s40850-022-00154-5","url":null,"abstract":"<p><strong>Background: </strong>Human-wildlife conflict (HWC) is forecasted to increase globally in the vicinity of protected areas and covers various dimensions. It occurs in several different contexts and involves a range of animal taxonomic groups where the needs and requirements intersect with humans' needs and development. More often, human-monkey conflict occurs in developing countries and is amongst the main threats to biodiversity conservation in these regions. Grivet monkeys are slender agile monkeys of the genus Cercopithecus. This study was conducted to investigate the status of human grivet monkey conflict and the attitude of local communities towards grivet monkey conservation in and around Wof-Washa Natural State Forest (WWNSF), Ethiopia from September 2017 to May 2018. A questionnaire survey (143 respondents) was used to study the human-grivet monkey conflict and its conservation status.</p><p><strong>Results: </strong>The majority of respondents (male = 67.1%; female = 74.1%) were not supporting grivet monkey conservation due to the troublesome crop-damaging effect of the animal. Respondents having settlements/farmland nearer to the forest have significantly negative perceptions towards grivet monkey conservation than those far from it. The majority of respondents opined that eradication/relocation of grivet monkeys and financial compensation are the options to mitigate human-grivet monkey conflict. Based on the questionnaire result, 42.5 ± SD 8.68 of respondents in all villages elucidated that the main cause of crop damage by grivet monkeys was habitat degradation.</p><p><strong>Conclusion: </strong>In the study area, Human-Grivet Monkey Conflict (HGMC) is exacerbated by the encroachment of local communities into the forest area, exploitation of resources that would be used by grivet monkeys, and enhanced crop damage by grivet monkeys. As a result grivet monkeys have been persecuted as a consequence of crop damage. This was due to the negative attitude developed from human perspective. Thus, awareness creation education programs and feasible crop damage prevention techniques need to be implemented.</p>","PeriodicalId":48590,"journal":{"name":"BMC Zoology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9447067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary morphology of sperm in pholcid spiders (Pholcidae, Synspermiata).","authors":"Tim M Dederichs,&nbsp;Bernhard A Huber,&nbsp;Peter Michalik","doi":"10.1186/s40850-022-00148-3","DOIUrl":"https://doi.org/10.1186/s40850-022-00148-3","url":null,"abstract":"<p><strong>Background: </strong>Pholcidae represent one of the largest and most diverse spider families and have been subject to various studies regarding behavior and reproductive biology. In contrast to the solid knowledge on phylogeny and general reproductive morphology, the primary male reproductive system is strongly understudied, as it has been addressed only for few species. Those studies however suggested a high diversity of sperm and seminal secretions across the family. To address this disparity and reconstruct the evolution of sperm traits, we investigate the primary male reproductive system of pholcid spiders by means of light, X-ray, and transmission electron microscopy using a comprehensive taxon sampling with 46 species from 33 genera, representing all five subfamilies.</p><p><strong>Results: </strong>Our data show a high disparity of sperm morphology and seminal secretions within pholcids. We document several sperm characters that are unique for pholcids, such as a helical band (Pholcinae) or a lamellate posterior centriolar adjunct material (Modisiminae). Character mapping revealed several putative synapomorphies for individual taxa. With regard to sperm transfer forms, we found that synspermia occur only in the subfamily Ninetinae, whereas the other subfamilies have cleistospermia. In several species with cleistospermia, we demonstrate that spermatids remain fused until late stages of spermiogenesis before ultimately separating shortly before the coiling process. Additionally, we explored the previously hypothesized correlation between sperm size and minimum diameter of the spermophor in the male palpal organ. We show that synspermia differ strongly in size whereas cleistospermia are rather uniform, but neither transfer form is positively correlated with the diameter of the spermophor.</p><p><strong>Conclusions: </strong>Our data revealed a dynamic evolution of sperm characters, with convergences across all subfamilies and a high level of homoplasy. The present diversity can be related to subfamily level and allows for assignments of specific subtypes of spermatozoa. Our observations support the idea that Ninetinae are an ancestral clade within Pholcidae that have retained synspermia and that synspermia represent the ancestral sperm transfer form of Pholcidae.</p>","PeriodicalId":48590,"journal":{"name":"BMC Zoology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9452986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solving a running crab spider puzzle: delimiting Cleocnemis Simon, 1886 with implications on the phylogeny and terminology of genital structures of Philodromidae.","authors":"André Wanderley do Prado,&nbsp;Renner Luiz Cerqueira Baptista,&nbsp;Hector Baruch Pereira Schinelli,&nbsp;Daniela Maeda Takiya","doi":"10.1186/s40850-022-00136-7","DOIUrl":"https://doi.org/10.1186/s40850-022-00136-7","url":null,"abstract":"<p><strong>Background: </strong>Among the 16 Neotropical genera of Philodromidae, Cleocnemis has the most troublesome taxonomic situation. Remarkable morphological differences among several genera historically said to be related to Cleocnemis denote controversial notions and general uncertainty about the genus identity. Thus, to clarify the genus limits and contribute to the understanding of Neotropical Philodromidae, we conducted a morphological analysis, along with Bayesian Inference and Maximum Likelihood molecular phylogenetic analyses focusing on Cleocnemis and related genera of Thanatinae. All of the 14 species previously placed in Cleocnemis were studied, and eight of them included in the molecular analyses based on fragments of 28S rDNA, histone H3, 16S rDNA, and cytochrome oxidase I (COI).</p><p><strong>Results: </strong>Cleocnemis was recovered as polyphyletic. Most of its species are distributed into six lineages allocated into five morphologically recognizable groups: Group I [Cleocnemis heteropoda], representing Cleocnemis sensu stricto and two new junior synonyms, Berlandiella and Metacleocnemis; Group II [Tibelloides bryantae comb. nov., Tibelloides punctulatus comb. nov., Tibelloides reimoseri nom. nov., and Tibelloides taquarae comb. nov.], representing Tibelloides gen. rev., which was not recovered as monophyletic; Group III [Fageia moschata comb. nov., Fageia rosea comb. nov.], representing the genus Fageia; Group IV [\"Cleocnemis\" lanceolata]; and Group V [\"Cleocnemis\" mutilata, \"Cleocnemis\" serrana, and \"Cleocnemis\" xenotypa]. Species of the latter two groups are considered incertae sedis. Cleocnemis spinosa is maintained in Cleocnemis, but considered a nomen dubium. Cleocnemis nigra is considered both nomen dubium and incertae sedis. We provide a redelimitation of Cleocnemis, redescription, neotype designation, and synonymy of type-species C. heteropoda. Taxonomic notes on composition, diagnosis, and distribution for each cited genus are also provided. Phylogenetic results support the division of Philodromidae into Thanatinae new stat. and Philodrominae new stat. and suggest expansion of their current compositions. Terminology of genital structures of Philodromidae is discussed.</p><p><strong>Conclusions: </strong>Our results bring light to Cleocnemis taxonomy and enhance the understanding of the relationships within Philodromidae, especially through the assessment of neglected Neotropical taxa.</p>","PeriodicalId":48590,"journal":{"name":"BMC Zoology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9823960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Pleistocene climate fluctuations on the demographic history and distribution of the critically endangered Chinese pangolin (Manis pentadactyla).","authors":"Shichao Wei,&nbsp;Song Sun,&nbsp;Hongliang Dou,&nbsp;Fuyu An,&nbsp;Haiyang Gao,&nbsp;Ce Guo,&nbsp;Yan Hua","doi":"10.1186/s40850-022-00153-6","DOIUrl":"https://doi.org/10.1186/s40850-022-00153-6","url":null,"abstract":"<p><strong>Background: </strong>Pleistocene climate fluctuations have strongly modified species genetic diversity and distributions. The Chinese pangolin (Manis pentadactyla) has been recognized as a critically endangered animal due to heavy poaching and trafficking. However, the effect of Pleistocene climate fluctuations on the genetic diversity and spatial distribution of the Chinese pangolin remains largely unknown. Here, we combined whole genome sequencing data, analysis of complete mitochondrial genomes, and a large amount of occurrence data from field surveys to infer the ancestral demographic history and predict the past spatial dynamics of the Chinese pangolin in Guangdong Province, China.</p><p><strong>Results: </strong>Our results indicated that there were two subpopulations, which showed similar trends of population size change in response to past climatic changes. We estimated a peak effective population size (N_e) during the last interglacial (LIG), followed by a marked decrease (~ 0.5 to fivefold change) until the last glacial maximum (LGM) and a rebound to a small peak population size during the Mid-Holocene (MH). The estimated time of the separation event between two subpopulations was approximately 3,000-2,500 years ago (ka). We estimated that the distribution of suitable areas shrank by 14.4% from the LIG to LGM, followed by an expansion of 31.4% from the LGM to MH and has been stable since then. In addition, we identified an elevational shift and suitable area decreased significantly during the LGM, but that the geographic extent of suitable areas in the western region increased from the LIG to present. The eastern region of Guangdong Province had the highest habitat suitability across all the climate scenarios.</p><p><strong>Conclusions: </strong>Our results suggested that Pleistocene climate fluctuations played an important role in shaping patterns of genetic diversity and spatial distribution, and that human stressors likely contributed to the recent divergence of two Chinese pangolin subpopulations sampled here. We argue that a key protected area should be established in the eastern region of Guangdong Province. As such, this study provides a more thorough understanding of the impacts of Pleistocene climate fluctuations impacts on a mammalian species in southern China and suggests more robust management and conservation plans for this Critically Endangered species of special interest.</p>","PeriodicalId":48590,"journal":{"name":"BMC Zoology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9806965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D render volume CT reconstruction of the bones and arteries of the hind digit of the dromedary camel (Camelus dromedarius).","authors":"Samir A A El-Gendy,&nbsp;Basma M Kamal,&nbsp;Mohamed A M Alsafy","doi":"10.1186/s40850-022-00151-8","DOIUrl":"https://doi.org/10.1186/s40850-022-00151-8","url":null,"abstract":"<p><strong>Background: </strong>The 3D computed tomography produces detailed images of the digit bones in addition to the angiograph render volume 3D of the CT shows the relation between the arteries, bones, and tissues of the digit. Therefore, the present study used those imaging techniques to provide a complete description of the digit bones and arteries' origin, distribution, and course and their relations with surrounding structures in the Dromedary Camel. Which would serve as a guide for surgeons and students in distinguishing normal digit structures. The study used eight hind limbs of four adult camels of both sexes (two males and two females), aged 9-15 years (Mean ± SD, 11.80 ± 2.59 years). The samples were injected with latex with lead oxide were undergone 3D render volume CT (128-slice multi-detector CT scanning protocol) and angiography x-rays.</p><p><strong>Results: </strong>The blood vessels and correlated structures such as bones, tendons, and ligaments were examined using 3D CT in all directions, which was easier to view than angiography and dissected specimens. The arterial supply to the camel's hind foot was the A. digitalis plantaris communis III. The angiography render volume 3D of CT explained the blood supply of the bones and joints of digital regions and showed a good visualization of the many digit arteries. The metatarsals, the phalanges, and the sesamoid bones were visualized. A. plantaris medialis superficialis, A. digitalis plantaris communis III, A. digitalis plantaris communis II and IV, A. interdigitalis, rami articularis medialis and lateralis to the fetlock joint, ramus medialis and ramus lateralis of the A. digitalis plantaris communis III, A. digitalis plantaris propriae III et IV abaxialis, A. digitalis plantaris propriae III et IV axialis, Ramus phalangis axialis and abaxialis of the first phalanx, Ramus phalangis axialis and abaxialis of the second and third phalanges, and A. metatarsae plantaris III were visualized. The study discovered new blood vessel sources in dromedary camels, such as the ramus articularis to the fetlock and the ramus plantaris phalangis abaxialis proximalis and distalis of the first phalanx.</p><p><strong>Conclusions: </strong>The digital circulation angiography investigates the circulatory pattern of the camel hind digit, which can assist clinicians in diagnosing digit region affections. 3D CT explained improved visualization of bones and arteries, including many small branches in relation to surrounding structures, in some views better than others.</p>","PeriodicalId":48590,"journal":{"name":"BMC Zoology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10127028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9446626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}