Pakistan journal of biotechnology最新文献_第2页

COMPARATIVE EFFECT OF HYDRO AND HALOPRIMING ON SEED GERMINATION AND SEEDLING GROWTH OF IPIL IPIL (Leucaena leucocephala) 水分和光照对印度罂粟（Leucaena leucocephala）种子发芽和幼苗生长的比较影响

Pakistan journal of biotechnology Pub Date : 2024-02-19 DOI: 10.34016/pjbt.2024.21.01.856

Sana Shazia, Noor-un-nisa Memon, Niyaz Ahmed Wahocho, Muhammad Mithal Lund, Urooj Mithal Jiskani, M. I. Majeedano, Hube Ali Magsi, Abrar Mithal Jiskani, Afifa Talpur

{"title":"COMPARATIVE EFFECT OF HYDRO AND HALOPRIMING ON SEED GERMINATION AND SEEDLING GROWTH OF IPIL IPIL (Leucaena leucocephala)","authors":"Sana Shazia, Noor-un-nisa Memon, Niyaz Ahmed Wahocho, Muhammad Mithal Lund, Urooj Mithal Jiskani, M. I. Majeedano, Hube Ali Magsi, Abrar Mithal Jiskani, Afifa Talpur","doi":"10.34016/pjbt.2024.21.01.856","DOIUrl":"https://doi.org/10.34016/pjbt.2024.21.01.856","url":null,"abstract":"An experiment was conducted during the year 2020 to investigate the comparative effect of hydro and halopriming on seed germination and seedling growth of Ipil Ipil. The seeds of Ipil Ipil were primed in various solutions for 30 hours. The seeds were soaked in priming solutions viz. distilled water, luke warm water, NaCl solution @ 1% and 2%. The unprimed seeds were taken as control. The data was recorded for seed germination percentage, mean germination time (MGT), germination index (GI), seedling vigor index (SVI), fresh biomass of shoot, fresh biomass of roots, dry biomass of shoot, dry biomass of roots, quality index of the seedlings, sturdiness quotient and leakage of the electrolytes. The results revealed that all the observed parameters studied in the present study were significantly (P<0.05) influenced by various seed priming treatments. The results of seed priming depict that seed germination (90.00%), germination index (0.43), germination time (5.22 days) and seedling vigor index (1498.0) were observed better in response to the luke warm water. While the seeds primed with NaCl @ 1% had better results for fresh biomass of shoot (5.58 g), root (1.44 g), dry biomass of shoot (1.57 g) and root (0.29 g). Quality index of the seedlings and sturdiness quotient had better result from NaCl treatment. The quality of the seedlings was measured in terms of sturdiness quotient, quality index of the seedlings and leakage of the electrolytes. The sturdiness quotient (0.98), quality index of the seedling (24.85) was also better in response to the halo (NaCl) seed priming @ 1%. The only leakage of the electrolytes had no significant of seed priming. It is concluded from the present study that all seed germination related parameters are greatly affected by the lukewarm water treatment. However, parameters related with the seedling growth are affected by the NaCl treatment @ 1%.","PeriodicalId":485309,"journal":{"name":"Pakistan journal of biotechnology","volume":"219 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140451269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GEOMETRY OF THE GIN-SAW TEETH EFFECT ON SEPRATION OF FIBERS FROM SEED COTTON DURING THE GINNING PROCESS. 轧棉过程中，轧棉锯齿的几何形状对籽棉纤维分离的影响。

Pakistan journal of biotechnology Pub Date : 2024-02-18 DOI: 10.34016/pjbt.2024.21.01.887

Inamova Maftuna Dedamirza, Noorullah Soomro, Kamola Ortiqova Insopaliyevna, Jurayeva Gulkhayo Ravshanbekovna, Mirzaakbarov Azizbek Avaz ugli

引用次数: 0

INTELLIGENT AGRICULTURAL PEST MANAGER DRONE IN PAKISTAN 巴基斯坦的智能农业害虫管理无人机

Pakistan journal of biotechnology Pub Date : 2023-10-10 DOI: 10.34016/pjbt.2023.20.02.816

Shahzad Nasim, Munaf Rashid, Sidra Abid Syed, Imtiaz Brohi

{"title":"INTELLIGENT AGRICULTURAL PEST MANAGER DRONE IN PAKISTAN","authors":"Shahzad Nasim, Munaf Rashid, Sidra Abid Syed, Imtiaz Brohi","doi":"10.34016/pjbt.2023.20.02.816","DOIUrl":"https://doi.org/10.34016/pjbt.2023.20.02.816","url":null,"abstract":"This paper’s primary goal is to develop an agriculture drone for spraying pesticides. We discuss an architecture based on unmanned aerial vehicles (UAVs) in this study. Pesticides must be used in agriculture if the quality of large-scale output is to be maintained. It is crucial to increase agriculture's production and efficiency by employing cutting-edge technology to replace employees with intelligent equipment like robots. The research suggests a novel method to replace people in a number of agricultural tasks, including the identification of insect presence, the application of pesticides and fertilizers, etc. The created method entails building a prototype that makes use of basic, affordable equipment including a microprocessor, different motors, and terminal equipment to assist farmers in a variety of operations related to crop fields. Design and build an autonomous drone-based surveillance system capable of identifying injured crops and spraying pesticides in specified regions as needed. To combine an image processing and Artificial Intelligence based real time algorithm to determine crop health and evaluate the need for pesticides, as well as a weather monitoring system that can assist anticipate weather conditions","PeriodicalId":485309,"journal":{"name":"Pakistan journal of biotechnology","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136358658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EXTRACTION AND UTILIZATION OF BANANA PSEUDOSTEM SAP AS ORGANIC LIQUID BIO-FERTILIZER ON ONION (ALLIUM CEPA L) 香蕉假茎SAP作为有机液体生物肥料在洋葱上的提取与利用

Pakistan journal of biotechnology Pub Date : 2023-10-08 DOI: 10.34016/pjbt.2023.20.02.839

Shaukat Ali Abro, Manzoor Ali Abro, Jabir Husain Kapri, Nisar Ahmed, Hamz Ali, Shazia Parveen, Mumtaz Ali, Sameer Ali, Asma Khoso

{"title":"EXTRACTION AND UTILIZATION OF BANANA PSEUDOSTEM SAP AS ORGANIC LIQUID BIO-FERTILIZER ON ONION (ALLIUM CEPA L)","authors":"Shaukat Ali Abro, Manzoor Ali Abro, Jabir Husain Kapri, Nisar Ahmed, Hamz Ali, Shazia Parveen, Mumtaz Ali, Sameer Ali, Asma Khoso","doi":"10.34016/pjbt.2023.20.02.839","DOIUrl":"https://doi.org/10.34016/pjbt.2023.20.02.839","url":null,"abstract":"The high input cost of onion production through the application of chemical fertilizers is a significant concern for small farmers. Every year, Banana harvesting generates a large amount of banana pseudostem waste, which is generally burnt or thrown away despite containing many nutrients after harvest. Utilizing the banana's pseudo-stem sap as liquid fertilizer for increasing onion productivity through sustainable agricultural practices, a greenhouse trial was conducted to determine the optimum banana pseudostem sap (BPS) to be applied by foliar spray. There were five treatments designed in CRD with four replicates. The reduced mineral fertilizer was given to test the impact of the pseuodstem sap on the seedlings. The results indicated that sap application showed a positive and significant effect on all parameters of onion seedlings. The onion plant height increased to 48.96 cm, the number of leaves was 14.50, and the shoot length of 8.67 cm was recorded best. The onion bulb diameter ranged from 3 to 6 cm bulb weight from 54 to 92.75 g comparing the control. The best rate found was 80% RDF, along with 20% BSP in all parameters. It can be concluded that application of 80% RDF + Foliar spray of 20% banana pseudostem sap was found to be the most viable option for getting higher growth and yield in onion. This will reduce 20% chemical fertilizer and about 25% increase in the yield. This will be an eco-friendly practice and sustainable ergonomically, economically and environmentally.","PeriodicalId":485309,"journal":{"name":"Pakistan journal of biotechnology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135251724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

ADVANCEMENT OF CROP PRODUCTIVITY VIA CRISPR-NANOPARTICLE INTERFACE 利用crispr -纳米颗粒界面提高作物生产力

Pakistan journal of biotechnology Pub Date : 2023-09-20 DOI: 10.34016/pjbt.2023.20.02.826

Amir Afzal, Sairah Syed, Mishal Khizar, Javed Iqbal, Sharmin Ashraf, Aneesa Altaf, Basharat Mehmood, Muhammad Rashid Khan

{"title":"ADVANCEMENT OF CROP PRODUCTIVITY VIA CRISPR-NANOPARTICLE INTERFACE","authors":"Amir Afzal, Sairah Syed, Mishal Khizar, Javed Iqbal, Sharmin Ashraf, Aneesa Altaf, Basharat Mehmood, Muhammad Rashid Khan","doi":"10.34016/pjbt.2023.20.02.826","DOIUrl":"https://doi.org/10.34016/pjbt.2023.20.02.826","url":null,"abstract":"Plant improvement strategies involve diverse techniques, ranging from traditional to marker-assisted methods, as well as chemical and radiation treatments. However, these methods can introduce imprecise changes in plant DNA. Accelerating plant enhancement is crucial to meet global food demand, but current methods are time-consuming. Scientists are revolutionizing plant breeding by employing various techniques to develop crops with specific attributes, such as increased yield and pest resistance, aligning with environmental and societal needs. While these methods offer substantial advantages, they often face challenges and can be less precise than desired. Innovative methods, such as gene editing using CRISPR, offer enhanced precision. CRISPR technology enables precise modifications to a plant's DNA, allowing for targeted improvements without unintended consequences. While CRISPR shows great potential, ensuring its safe and accurate implementation is a priority. Scientists are exploring diverse methods, both viral and non-viral, to effectively deliver CRISPR components into plant cells, with non-viral approaches gaining traction due to their safety and versatility. Nanoparticles play a pivotal role in these advancements by serving as delivery vehicles for CRISPR tools. These particles safeguard and transport the necessary components to specific locations within plants, bolstering growth, yield, and disease resistance. Despite challenges, the synergy of nanotechnology and CRISPR holds promise for revolutionizing plant improvement while safeguarding the environment. This integrated approach offers the potential to enhance crop growth and quality while upholding ecological balance.","PeriodicalId":485309,"journal":{"name":"Pakistan journal of biotechnology","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136308910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STUDIES ON THE MORPHOLOGY AND MOLECULAR CHARACTERIZATION OF STORED GRAIN WEEVILS SITOPHILUS (CURCULIONIDAE: COLEOPTERA) FROM KHAIRPUR, SINDH-PAKISTAN 巴基斯坦信德省海普尔储粮象鼻虫形态与分子特征的研究

Pakistan journal of biotechnology Pub Date : 2023-09-19 DOI: 10.34016/pjbt.2023.20.02.819

Shahnoor Suhriani, Waheed Ali Panhwar, Abdul Manan Shaikh

引用次数: 0

GENETIC ANALYSIS IN VARIOUS GENOTYPES OF BREAD WHEAT UNDER NORMAL AND HEAT-STRESS ENVIRONMENTS 面包小麦不同基因型在正常和热胁迫环境下的遗传分析

Pakistan journal of biotechnology Pub Date : 2023-09-14 DOI: 10.34016/pjbt.2023.20.02.817

Raza Ali Rind, Shabana Memon, Wajid Ali Jatoi, Aijaz Ahmed Soomro

{"title":"GENETIC ANALYSIS IN VARIOUS GENOTYPES OF BREAD WHEAT UNDER NORMAL AND HEAT-STRESS ENVIRONMENTS","authors":"Raza Ali Rind, Shabana Memon, Wajid Ali Jatoi, Aijaz Ahmed Soomro","doi":"10.34016/pjbt.2023.20.02.817","DOIUrl":"https://doi.org/10.34016/pjbt.2023.20.02.817","url":null,"abstract":"Abiotic stresses have brought the crops to a destructive position towards yield production of crops, especially wheat. The present study was investigated to compare the relationship between normal and heat stress conditions under two different sowing dates viz. normal and late sowing dates (25th Nov and 25th Dec). The correlation coefficients varied with both sowing dates (normal and late planting). In normal planting number of grains showed a significant positive correlation with grain weight spike-1 (r = 0.618**), grain yield plant-1 (r = 0.591**), seed index (r =0 .456**), biological yield plant-1 (r = 0.540**) and harvest index (r =0 .667**). Grains spike-1 contributed significant positive correlation with grains spike-1 (r=0.094**), grain yield plant-1 (r=0.844**), biological yield plant-1 (r=0.936**), harvest index (r=0.556**), leaf area (r=0.791**), relative water content (r=0.763**), chlorophyll content (r=0.853**), cell membrane stability (r=0.828**) and stomatal conductance (r=0.292**). Grain yield plant-1 exhibited a significant positive correlation under normal planting with the number of tillers plant-1, number of spikelets spike-1, grains spike-1, and grain weight spike-1 (r=0.695**,0.207*,0.591**and 0.950**), respectively. Whereas, late planting declared grains spike-1 revelaed signficant positive correlation with grains spike-1 (r=0.094**), grain yield plant-1 (r=0.844**), biological yield plant-1 (r=0.936**), harvest index (r=0.556**), leaf area (r=0.791**),relative water content (r=0.763**), chlorophyll content (r=0.853**), cell membrane stability (r=0.828**) and stolatal conductance (r=0.292**). Under late planting, Yield showed a significant positive correlation with spike length, grains spike-1, and grain weight spike-1 (r=0.343**,0.844**, and 0.964**), respectively","PeriodicalId":485309,"journal":{"name":"Pakistan journal of biotechnology","volume":"22-23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134971458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IDENTIFICATION OF HETERORRHINA SAEEDI SP.NOV. (SCARABAEIDAE: COLEOPTERA) FROM SINDH REGION, PAKISTAN 异花蛇属植物的鉴定。(金龟科:鞘翅目)产于巴基斯坦信德地区

Pakistan journal of biotechnology Pub Date : 2023-08-31 DOI: 10.34016/pjbt.2023.20.02.818

Mehtab Ali Mahar, Waheed Ali Panhwar, Abdul Manan Shaikh

引用次数: 0

PHYTOMORPHOLOGY OF WHEAT AND ITS GRAIN QUALITY RESPONSE TO DIVERSE APPROACHES OF IRON APPLICATION 小麦植物形态及其品质对不同施铁方式的响应

Pakistan journal of biotechnology Pub Date : 2023-06-11 DOI: 10.34016/pjbt.2023.20.02.801

Muhammad Yousif Shaikh, Aijaz Ahmed Soomro, Muhammad Nawaz Kandhro, Inayatullah Rajpar

{"title":"PHYTOMORPHOLOGY OF WHEAT AND ITS GRAIN QUALITY RESPONSE TO DIVERSE APPROACHES OF IRON APPLICATION","authors":"Muhammad Yousif Shaikh, Aijaz Ahmed Soomro, Muhammad Nawaz Kandhro, Inayatullah Rajpar","doi":"10.34016/pjbt.2023.20.02.801","DOIUrl":"https://doi.org/10.34016/pjbt.2023.20.02.801","url":null,"abstract":"Agriculture soils of Pakistan are already deficient of macronutrients, and now inadequacy for micronutrients has also been reported causing adverse effects on crop productivity. The study was carried out to investigate the effect of soil and foliar applied iron (Fe) on wheat. The treatments included: T1=Control (without Fe), T2=Soil applied Fe 3 kg ha‑1, T3=Soil applied Fe 6 kg ha‑1, T4=Foliar applied Fe 0.2%, T5=Foliar applied Fe 0.4%, T6=Soil applied Fe 1.5 kg ha-1+foliar applied Fe 0.1%, T7=Soil applied Fe 1.5 kg ha-1+foliar applied Fe 0.2%, T8=Soil applied Fe 3 kg ha-1+foliar applied Fe 0.1% and T9=Soil applied Fe 3 kg ha-1+foliar applied Fe 0.2%. All the treatments as alone or in combination, affected growth, yield and quality of wheat significantly (P<0.05). The results showed that T9 and T3 were most promising for almost all studied traits. Under T9 and T3, variety “TD-1” produced plants of 59.44 and 59.09 cm, while “Sindhu” showed 92.9 and 75.98 cm height, respectively. Higher grain yield (4799.7 and 4760.5 kg ha-1), grain protein (17.22 and 17.13%) were also recorded in T9 and T3, respectively; while T1 remained the least. In varieties, “TD-1” produced higher grain yield (4788.9 kg) than “Sindhu” (4341.8 kg ha-1). Inversely, grain protein was higher in variety “Sindhu” (17.10%) than “ TD-1” (16.72%). It was concluded that soil applied Fe 3 kg ha-1+foliar Fe 0.2% concentration or in absence of foliar application, soil applied Fe at 6 kg ha-1 remained most appropriate dose for wheat growers in addition to recommended NPK","PeriodicalId":485309,"journal":{"name":"Pakistan journal of biotechnology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135004270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CLINICAL MANIFESTATION OF HEPATITIS C AMONG THE POPULATION OF HYDERABAD, PAKISTAN 巴基斯坦海得拉巴人口中丙型肝炎的临床表现

Pakistan journal of biotechnology Pub Date : 2023-06-11 DOI: 10.34016/pjbt.2023.20.02.785

None Mairaj Bibi, None Asghar Ali, Uroosa Sahar, Abdul Sajid, Santosh Kumar, Zameer Ali Palh, None Syed Habib Ahmed Naqvi, Shahla Karim Baloch

引用次数: 0