M Brejawi, A Venkiteswaran, S M O Ergieg, B Md Sabri
{"title":"The need for a comprehensive case-severity-grading tool for molar-incisor hypomineralisation (MIH).","authors":"M Brejawi, A Venkiteswaran, S M O Ergieg, B Md Sabri","doi":"10.1007/s40368-024-00903-9","DOIUrl":"10.1007/s40368-024-00903-9","url":null,"abstract":"","PeriodicalId":47603,"journal":{"name":"European Archives of Paediatric Dentistry","volume":" ","pages":"607-608"},"PeriodicalIF":2.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y Y L Lai, J Downs, S Leishman, H M Leonard, L J Walsh, S Zafar
{"title":"qPCR assay optimisation for a clinical study comparing oral health risk in Rett syndrome.","authors":"Y Y L Lai, J Downs, S Leishman, H M Leonard, L J Walsh, S Zafar","doi":"10.1007/s40368-024-00912-8","DOIUrl":"10.1007/s40368-024-00912-8","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to validate qPCR assays for specific microbiota, for use on dental plaque samples stored on Whatman FTA cards to compare relative oral health risk in Rett syndrome.</p><p><strong>Methods: </strong>Supragingival dental plaque samples were collected, using a sterile swab, (COPAN FLOQswab™) swabbed onto Whatman FTA™ cards. DNA extraction was performed using a modified Powersoil™ protocol. Where published assays were unsuitable, species-specific qPCR assays for caries-associated, gingivitis-associated and oral-health-associated bacteria were designed using multiple sequence alignment, Primer3Plus and PrimerQuest. Assays were run using absolute quantification. Limit of detection (LOD) and limit of quantification (LOQ) were calculated, and PCR products verified by Sanger sequencing.</p><p><strong>Results: </strong>Most assays allowed detection using real-time qPCR with high specificity on samples collected on FTA cards. Several assays showed low or even single gene copy numbers on the test samples.</p><p><strong>Conclusion: </strong>Assays were optimised for detection and evaluation of oral health risk in dental plaque samples stored on FTA cards when cold storage is not feasible, except for F. nucleatum. Several assays showed gene copy numbers less than the LOQ or outside the range of the standard curve, so there is merit in optimising these assays using digital droplet PCR.</p>","PeriodicalId":47603,"journal":{"name":"European Archives of Paediatric Dentistry","volume":" ","pages":"547-560"},"PeriodicalIF":2.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11341660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P Pengpue, V Sirimaharaj, P Chaijareenont, W Chinadet
{"title":"Tensile bond strength between paediatric prefabricated zirconia crowns and primary maxillary incisors when using various types of luting cements: an in vitro study.","authors":"P Pengpue, V Sirimaharaj, P Chaijareenont, W Chinadet","doi":"10.1007/s40368-024-00890-x","DOIUrl":"10.1007/s40368-024-00890-x","url":null,"abstract":"<p><strong>Purpose: </strong>There is limited evidence regarding the most appropriate type of luting cement for paediatric prefabricated zirconia crowns (PZCs) in primary maxillary incisors. The retention of PZCs is dependent on the bond strength of luting cement between PZCs and primary maxillary incisors. The aim of this study was to evaluate the tensile bond strengths between PZCs and primary maxillary incisors with different types of luting cements.</p><p><strong>Methods: </strong>Thirty freshly extracted human primary maxillary incisors were prepared and randomly divided into three groups corresponding to three luting cements: bioactive cement, resin cement, and resin-modified glass ionomer cement (RMGIC), and then restored with PZCs. Tensile bond strengths were evaluated by a universal testing machine. The results were analysed using one-way ANOVA with Tukey's post-hoc test (p < 0.05).</p><p><strong>Results: </strong>The means of the tensile bond strengths were 1.43 ± 0.85 MPa, 0.91 ± 0.63 MPa, and 0.56 ± 0.39 MPa for the bioactive cement, resin cement, and RMGIC groups, respectively. A significant difference in tensile bond strength was observed between the bioactive cement and the RMGIC group (p < 0.05) but there was no significant difference in tensile bond strength between the resin cement group and the others.</p><p><strong>Conclusion: </strong>Types of luting cement influenced the tensile bond strength between PZCs and primary maxillary incisors. The bioactive cement showed higher tensile bond strength than the resin cement and RMGIC.</p>","PeriodicalId":47603,"journal":{"name":"European Archives of Paediatric Dentistry","volume":" ","pages":"471-479"},"PeriodicalIF":2.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140868575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M S Fernandez, L A Pauli, A B G Araújo, G T Demarco, M S Azevedo, V P P da Costa, M L Goettems
{"title":"Dental pain in Brazilian preschool children: association with the severity of dental caries and impact on oral health-related quality of life.","authors":"M S Fernandez, L A Pauli, A B G Araújo, G T Demarco, M S Azevedo, V P P da Costa, M L Goettems","doi":"10.1007/s40368-024-00906-6","DOIUrl":"10.1007/s40368-024-00906-6","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to verify the association between dental pain and severity of dental caries (caries morbidity stages) and the impact on oral health-related quality of life (OHRQoL) in preschool children.</p><p><strong>Methods: </strong>A cross-sectional study with 199 children (2-5 years old) enrolled at preschools in Capão do Leão-RS, Brazil. The self-report of mothers of children with a history of dental pain in the last 6 months and perception of their child's OHRQoL (ECOHIS) were obtained through a structured questionnaire. This questionnaire also collected independent variables. Children's oral examination was performed using the CAST instrument to determine caries morbidity stage. Crude and adjusted Poisson regression analysis was performed.</p><p><strong>Results: </strong>The prevalence of dental pain was 14.57%. The chance of the occurrence of dental pain was higher among children diagnosed in morbidity [Prevalence ratio-PR: 5.29 (95% confidence interval-95% CI 1.91-14.61); p = 0.001] and severe morbidity [RP = 6.12 (95 CI% 2.25-16.64); p < 0.001] stages. Children with dental pain presented higher scores in the total ECOHIS [rate ratio = 7.11 (95% CI 4.55-11.09); p < 0.001] and in all of the domains of this instrument. Furthermore, children with a history of dental trauma [PR = 2.41 (95% CI 1.15-5.04); p < 0.001] and those whose reason for last visit to the dental office was for restorative/endodontic/extraction treatment [PR = 1.29 (95% CI 1.01-6.19); p = 0.049] had a higher prevalence of dental pain.</p><p><strong>Conclusion: </strong>A substantial prevalence of dental pain in the last 6 months and negative impact on children's OHRQoL was identified in this sample. Children diagnosed with carious dentin lesions and abscess and fistula were more likely to have dental pain.</p>","PeriodicalId":47603,"journal":{"name":"European Archives of Paediatric Dentistry","volume":" ","pages":"481-490"},"PeriodicalIF":2.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140960264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hypomineralised second primary molars: the Würzburg concept.","authors":"K Bekes, R Steffen, N Krämer","doi":"10.1007/s40368-024-00913-7","DOIUrl":"10.1007/s40368-024-00913-7","url":null,"abstract":"<p><strong>Purpose: </strong>In addition to molar incisor hypomineralisation, the occurrence of enamel hypomineralisation in the primary dentition has become increasingly important in recent years. Hypomineralised second primary molar (HSPM) is defined as hypomineralisation of systemic origin affecting from one to all four second primary molars. Some years ago, the \"Würzburg concept\" was introduced, which proposed a grading of MIH findings (MIH treatment need index) in combination with an appropriate treatment plan depending on the severity of the affected tooth. Recently, this concept was updated and new treatment approaches have been added. However, currently, the concept solely addresses the treatment plan for permanent teeth. As there is a need to expand its scope to encompass primary teeth and, consequently, HSPM, this paper seeks to develop the second component of the Würzburg concept, the treatment plan, for the primary dentition in response to the increased focus on the disease in recent years. Although the evidence base for the different treatment options is still weak, there is a need for guidance for clinicians in their day-to-day practice.</p><p><strong>Methods: </strong>The authors conducted a comprehensive review of the literature, encompassing clinical and laboratory studies along with published guidelines.</p><p><strong>Results: </strong>The treatment plan of the HSPM Würzburg concept contains prophylactic and regenerative aspects, non-invasive interventions, temporary and permanent restorative techniques, and extraction.</p><p><strong>Conclusions: </strong>The intention is to provide practical guidance to practitioners, acknowledging the necessity for further validation through clinical trials.</p>","PeriodicalId":47603,"journal":{"name":"European Archives of Paediatric Dentistry","volume":" ","pages":"597-602"},"PeriodicalIF":2.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11341634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141158584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M T Campos, L J do Nascimento, A K Barreira, V Colares
{"title":"Parenting styles and dental caries in Brazilian children and adolescents in foster care.","authors":"M T Campos, L J do Nascimento, A K Barreira, V Colares","doi":"10.1007/s40368-024-00909-3","DOIUrl":"10.1007/s40368-024-00909-3","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to analyse parenting styles as a possible factor associated with caries in foster children and adolescents who had been victims of domestic violence.</p><p><strong>Methods: </strong>The study was carried out in nine foster shelters in Recife, Northeast Brazil, with a sample of 88 participants aged 8 to 17 years old. Data were collected through intraoral examinations to verify deft/DMFT indices, applying the Demandingness and Responsiveness Scales to determine parenting styles. In turn, sociodemographic data were retrieved from records filed at the institutions. Descriptive analysis was performed, with the Mann-Whitney and Kruskal-Wallis tests being used to compare the categories of numerical variables.</p><p><strong>Results: </strong>The mean deft/DMFT was of 3.78 in children (8 and 9 years old) and 1.93 in adolescents (10 years old and older). Overall, the index was of 2.31. Neglect was the main reason for the children's admission to foster care, while neglectful was the most prevalent parenting style.</p><p><strong>Conclusion: </strong>Children of indulgent parents had higher caries indices in both deciduous and permanent teeth, while children of authoritative (permanent teeth) and authoritarian parents (deciduous teeth) had the lowest caries indices. However, no significant associations were found between caries and parenting styles.</p>","PeriodicalId":47603,"journal":{"name":"European Archives of Paediatric Dentistry","volume":" ","pages":"513-521"},"PeriodicalIF":2.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140910748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A da Silva Arduim, B W Stefanello, L I L Bonzanini, T L Lenzi, J B Hilgert, F N Hugo, L Casagrande
{"title":"Traumatic dental injuries and molar incisor hypomineralization: a cross-sectional study of schoolchildren from southern Brazil.","authors":"A da Silva Arduim, B W Stefanello, L I L Bonzanini, T L Lenzi, J B Hilgert, F N Hugo, L Casagrande","doi":"10.1007/s40368-024-00918-2","DOIUrl":"10.1007/s40368-024-00918-2","url":null,"abstract":"<p><strong>Purpose: </strong>To describe the prevalence and the variables associated with TDIs in schoolchildren aged 8 to 14 years in Estância Velha city in southern Brazil, placing emphasis on molar incisor hypomineralization.</p><p><strong>Methods: </strong>Students enrolled in public schools of a medium-sized city in the southern Brazil were selected using cluster random sampling. Clinical examinations assessed molar incisor hypomineralization-MIH (European Academy of Pediatric Dentistry criteria), dental caries (DMFT index), and TDIs (O'Brien's criteria). Socioeconomic and demographic variables were assessed using a standardized questionnaire. Prevalence ratios were estimated using Poisson regression (p < 0.05).</p><p><strong>Results: </strong>513 students (54.8% female), with a mean age of 11.6 (± 1.9) years, participated in the study. The prevalence of TDIs was 11.3%, with enamel fracture representing 90.4%. The high prevalence of traumatic dental injuries was associated with MIH (PR: 2.22 CI: 1.27; 3.87; p < 0.01) and overjet > 3 mm (PR: 2.03 CI 1.19; 3.45; p < 0.01).</p><p><strong>Conclusion: </strong>The sample of schoolchildren from southern Brazil had a low prevalence of traumatic dental injuries. Molar incisor hypomineralization and increased overjet were associated with the higher prevalence of traumatic dental injuries.</p>","PeriodicalId":47603,"journal":{"name":"European Archives of Paediatric Dentistry","volume":" ","pages":"569-575"},"PeriodicalIF":2.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141285013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Oral health and related outcomes in children and adolescents with cystic fibrosis: a scoping review.","authors":"D Chin, L Ramalingam, J Harrison, M Silva","doi":"10.1007/s40368-024-00885-8","DOIUrl":"10.1007/s40368-024-00885-8","url":null,"abstract":"<p><strong>Purpose: </strong>Good oral health is important for children and adolescents with cystic fibrosis (CF). The purpose of this scoping review is to describe the existing evidence base regarding oral health in children and adolescents with CF and provide recommendations for future research.</p><p><strong>Methods: </strong>Using a scoping review framework, a comprehensive search was undertaken using medline, embase, and PubMed. The search strategy included broad terms relating to CF, oral health, and children and adolescents and included only papers written in English.</p><p><strong>Results: </strong>61 articles were included. Topics investigated included dental caries, enamel defects, periodontal health, dental staining, oral health related quality of life, dental management, and dental development of children and adolescents with CF.</p><p><strong>Conclusion: </strong>Dental outcomes of children and adolescents with CF differ from the healthy population. The current literature describing dental health in children and adolescents with CF includes predominately descriptive analyses. A shift to hypothesis-based studies to explore causal relationships that explain the differences in dental outcomes seen in the CF population offers an opportunity to better understand the problems faced by children and adolescents with CF. Research that actively engages stakeholders, including children and adolescents with CF and their families will enable evidence-based recommendations to improve their oral health.</p>","PeriodicalId":47603,"journal":{"name":"European Archives of Paediatric Dentistry","volume":" ","pages":"455-469"},"PeriodicalIF":2.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11341622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative evaluation of shear bond strength and penetration of self-etch sealant with and without enamel deproteinization: an in vitro study.","authors":"M Agarwal, K D Gupta, R Yeluri","doi":"10.1007/s40368-024-00907-5","DOIUrl":"10.1007/s40368-024-00907-5","url":null,"abstract":"<p><strong>Aim: </strong>To compare the shear bond strength and penetration ability of self-etch sealant with and without enamel deproteinization.</p><p><strong>Materials and methods: </strong>A total of 37 sound extracted teeth were selected and 31 teeth were used for testing shear bond strength of self-etch sealant. For this, crowns portions of selected teeth were cut longitudinally with a diamond disc into a mesial and distal half, thereby yielding 62 samples. They were further divided into two groups; in Group 1, samples were deproteinised with 5.25% sodium hypochlorite and a sealant cylinder was built on the proximal surface. In Group 2, sealant cylinders were built without deproteinising the surface. Shear bond strength was tested using Universal Testing Machine. The remaining six teeth were used for penetration evaluation of self-etch sealant. In Group 1, the occlusal surface was deproteinised with 5.25% sodium hypochlorite and self-etch sealant was directly applied. In Group 2, sealant was applied without deproteinisation. The samples obtained after sectioning were observed under scanning electron microscope for sealant penetration. Mann-Whitney test and Chi-square analysis were utilised for statistical analysis.</p><p><strong>Results: </strong>The results showed that the shear bond strength of the two groups was statistically not significant (p = 0.23). However, the comparison of % penetration revealed a statistically significant difference between the groups (p = 0.016).</p><p><strong>Conclusion: </strong>Deproteinising the enamel surface prior to application of self-etch sealant enhanced the penetration ability of the sealant but did not show any effect on shear bond strength.</p>","PeriodicalId":47603,"journal":{"name":"European Archives of Paediatric Dentistry","volume":" ","pages":"491-500"},"PeriodicalIF":2.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140960307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Artificial tongue: a promising solution for early detection and treatment of oral bacterial infections.","authors":"E Veseli, E Behluli, E Breznica, A Veseli","doi":"10.1007/s40368-024-00899-2","DOIUrl":"10.1007/s40368-024-00899-2","url":null,"abstract":"","PeriodicalId":47603,"journal":{"name":"European Archives of Paediatric Dentistry","volume":" ","pages":"603-604"},"PeriodicalIF":2.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140869965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}