Saudi journal of pathology and microbiology最新文献

{"title":"Inhibition Rates of Disinfectants on Bacterial Isolated from Student Dormitory","authors":"O. Radhi","doi":"10.36348/sjpm.2024.v09i07.002","DOIUrl":"https://doi.org/10.36348/sjpm.2024.v09i07.002","url":null,"abstract":"Background: Although most of human life is spent inside, where bacteria are always present on things, little is known about the microbial variety of indoor habitats. Objectives: The major objective of this study was to determine the rate and types of bacterial contaminants in female and male student dormitory of Al-Kufa university campus, and to explore the inhibitory effect of certain disinfectants. This study was conducted for the period from October 2022 to Jun 2023. A total of 400 bacteriological swabs were collected aseptically from different sites of male and female student dormitory of Al-Kufa university. Swabs were streaked as soon as possible on blood agar and MaConkey agar plates and incubated overnight. Identification of bacterial species was based on standard bacteriological criteria. The inhibitory effect of 40% and 60% concentrations of three commercially available disinfectants; dettol (chloroxylenol), Al-Emlaq (Alkyl benzene sulfonate), and the house bleach (sodium hypochlorite) were explored by agar diffusion method. Data were statistically analyzed. Results: The results revealed that the overall bacterial contamination rate in student’s dormitories of Al-Kufa university campus was 78%. Male dormitory unit had significantly higher contamination rate compared to female dormitory unit (57.5% vs 42.5%). Bacillus spp. Was the most predominant contaminant in both male and female units. Al-Emlaq super gel (Alkyl benzene sulfonate) was found as the most effective disinfectant against the majority of bacterial isolates. Conclusion: Authorized and public awareness toward bacterial contamination in student's dormitories is recommended. The selection of an effective disinfectant should be based on its active ingredient and concentration.","PeriodicalId":471257,"journal":{"name":"Saudi journal of pathology and microbiology","volume":" 70","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141833840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of Microsatellite Instability in Colorectal Carcinoma- A Complete Review","authors":"Muthu Venkat T, Vijayalakshmi Vijayalakshmi, Pramila Pramila","doi":"10.36348/sjpm.2024.v09i03.003","DOIUrl":"https://doi.org/10.36348/sjpm.2024.v09i03.003","url":null,"abstract":"The microsatellite instability (MSI-H) or mismatch repair deficient (dMMR) colorectal tumors recently have been reported that can benefit from immunotherapy, and MSI can be used as a genetic instability of a tumor detection index. Many studies have shown that there are many heterogeneous phenomena in patients with MSI tumors in terms of immunotherapy, prognosis and chemotherapy sensitivity. Here we mainly review the research results of MSI detection methods, its mechanisms, occurrence and its relationship with related tumors, aiming in such a way for brief analysis of the micro satellite instability. Microsatellites (MS) are the repeated sequences of DNA that play an important role in maintaining the tissue morphology. Any mutation of the DNA or chromosomes, lead to the instability of the microsatellites, thereby causing the microsatellite instability. There are three types of microsatellite instability (MSI). High microsatellite instability (MSI-H), low microsatellite instability (MSI-L) and microsatellite stability (MSS). Recent clinical research tends to classify MSS-L and MSS as similar. Microstaellite instability plays an important role in colorectal carcinoma. Based on different molecular mechanisms, MSI in colorectal cancer can be divided into colorectal cancer (CRC) with no obvious family genetic history and Lynch syndrome with non-polyposis with family genetic history. Lynch syndrome is an autosomal dominant disorder and syndrome caused by mutations in MMR strains, and it can also cause tumors in other parts of the colon and rectum. With the recent development of MSI detection technology and immunosuppressant in tumor therapy, researchers found that MSI-H tumors respond well to immunotherapy. There are several methods to detect the microsatellite instability. 1. Next Generation sequencing (NGS), 2. Fluoresence multiplex PCR and capillary electrophoresis. 3. Immunohistochemistry. 4. Single molecule- molecular inversion probes (SmMIP). The main mechanism of MSI includes, Slipped strand mispairing, MMR deficient.","PeriodicalId":471257,"journal":{"name":"Saudi journal of pathology and microbiology","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140374736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Some Selected Medical Equipment in Olabisi Onabanjo University Teaching Hospital for Staphylococcus aureus of Nosocomial Threat","authors":"O. Lionel, Ade-Adekunle Olusegun Ayo, Kotun Bunmi Comfort, O. T. Oyedemi, Oyinloye O. Elijah, Caroline Olufunke Babalola, Kolade Titilayo Teniola, O. Adeleye","doi":"10.36348/sjpm.2024.v09i03.001","DOIUrl":"https://doi.org/10.36348/sjpm.2024.v09i03.001","url":null,"abstract":"Hospital is an establishment where patients that are diseased, infected, ill or injured receive medical care. A medical staff and equipment are required for the task of medical care delivery. The equipment that are employed in hospital could be classified as critical, semi-critical and non-critical depending on their design and tasks. A total of Eighty-five (85) samples of hospital equipment swab were obtained from Olabisi Onabanjo Teaching Hospital of which 40 samples of Staphylococcus aureus were isolated. The isolates were Gram stained followed by conventional biochemical test for the identification of Staphylococcus aureus. Antibiogram of the isolates was determined. The isolates exhibited resistance to ampiclox (87.5%), zinnacef (92.5%), amoxicillin (92.5%), rocephin (80%), and septrin (55%), while susceptible to pefloxacin (77.5%), gentamicin (80%), streptomycin (72.5%), and ciprofloxacin (52.5%). Some of the resistant isolates were exposed to plasmid DNA analysis and were found to be plasmid borne of varied molecular weight, which could be responsible for resistance to the antibiotics observed. There is therefore a need for regular disinfection, properly sterilization and preservation of medical equipment before and after use, which could curtailed or reduce the spread of equipment borne nosocomial infection.","PeriodicalId":471257,"journal":{"name":"Saudi journal of pathology and microbiology","volume":"58 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140080571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cupriavidus Pauculus as A Cause of Late Onset Post-Operative Sinusitis","authors":"K. Kosaraju, Mohammed Abdul Rahman Al Hashim, Ali Alabandi, Ruba Sarhan, Ruba Al Johani","doi":"10.36348/sjpm.2024.v09i02.006","DOIUrl":"https://doi.org/10.36348/sjpm.2024.v09i02.006","url":null,"abstract":"Cupriavidus pauculus is an environmental gram-negative bacilli of low virulence, being increasingly reported to be associated with invasive infections in immunocompromised patients and device related infections. Often undetected or misidentified in most microbiology laboratories, identification of this organism requires increased suspicion and automated identification or molecular methods. We hereby describe a curious case of post-operative acute sinusitis caused by this rare yet emerging human pathogen.","PeriodicalId":471257,"journal":{"name":"Saudi journal of pathology and microbiology","volume":"8 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140434779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of Pre-Analytical Errors in Laboratory & Steps to Improve","authors":"Reeta Grover, B. Gadhavi","doi":"10.36348/sjpm.2024.v09i01.005","DOIUrl":"https://doi.org/10.36348/sjpm.2024.v09i01.005","url":null,"abstract":"Objectives: Pre-analytical errors decisively influence the total laboratory errors and consequently the diagnostic accuracy. The following were the objectives of the study. 1) To detect the percentage of pre-analytical errors in venous specimens in Laboratory. 2) To categorize these pre-analytical errors. 3) To formulate steps of corrective measures to avoid such type of errors. Study Design and Result: Type of Study - Retrospective study. Study period - 12 months (June 2015 to May 2016), documenting the frequency and type of pre-analytical errors. Results: Total number of pre-analytical errors detected in the period of 12 months’ study were 180. Improper timing of specimens, hemolyzed & clotted specimens and improper requests were the major concerns followed by delay in specimen transport. Conclusion: Pre-analytical phase is an important component of Total Laboratory Quality. Pre-analytical errors are not inevitable and can be avoided or minimized with diligent application of quality control, continuing education, effective protocols, and standardized procedures for effective blood collection systems to ensure total Quality patient care.","PeriodicalId":471257,"journal":{"name":"Saudi journal of pathology and microbiology","volume":"137 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139604850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Prevalence of Hepatitis B Virus among Full-Term Mothers and their Infants at El-Obeid Teaching Hospital, Sudan","authors":"Eldaw Breima Suliman, Aaza Algyli Alloka, Haitham Abdalla Ali Ismail, Samira Mekki Ahmed Ali, Tamani Ahmed Bhar Ali, Nawal Ali Ahmed Mohammed, Hussain Gadelkarim Ahmed","doi":"10.36348/sjpm.2024.v09i01.003","DOIUrl":"https://doi.org/10.36348/sjpm.2024.v09i01.003","url":null,"abstract":"Background: The Hepatitis B virus causes dangerous liver illness. Infected pregnant women can infect their newborns and develop fulminant HBV. The study aimed to determine the prevalence of Hepatitis B virus among pregnant women and their newborns. Methodology: The study involved 226 randomly selected women from January to March 2023. Results: The results showed that 4.9% of pregnant women had HBV infection, with Caesarean Section being a significant risk factor. Around 27.3% of infected mothers transmitted HBV to their newborns. The age group 26-30 years had the highest prevalence rate of HBsAg, with urban residents having the highest prevalence. Conclusion: Caesarean section and urban resident were the most risk factors for infection with HBV. The study recommends laboratory-based screening and prophylactic vaccines to eliminate HBV from the general population.","PeriodicalId":471257,"journal":{"name":"Saudi journal of pathology and microbiology","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139611621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Appendicular Mucocele Complicated by Peritoneal Pseudomyxoma","authors":"Z. Amzal, M. Kadiri, M. Borahma, F. Chabib, N. Lagdali, C. Berhili, I. Benelbarhdadi, F. Ajana","doi":"10.36348/sjpm.2024.v09i01.004","DOIUrl":"https://doi.org/10.36348/sjpm.2024.v09i01.004","url":null,"abstract":"Appendiceal mucocele (AM) or Appendiceal mucinous neoplasms is a rare but potentially dangerous condition. In the latter, a spontaneous or iatrogenic rupture of the mucocele can lead to mucinous intraperitoneal ascites, a syndrome known as pseudomyxoma peritonei. We present the case of a 76-year-old female patient who reported right iliac fossa pain with RIF tenderness on clinical examination, and underwent an abdominal CT scan showing an appendiceal mucocele associated with a small peritoneal effusion suspecting a ruptured mucocele, related to peritoneal pseudomyxoma. The patient underwent appendectomy with peritoneal lavage and aspiration of all mucin found. Anatomopathological analysis confirmed the diagnosis of appendiceal mucocele (a low-grade appendiceal mucinous neoplasm).","PeriodicalId":471257,"journal":{"name":"Saudi journal of pathology and microbiology","volume":"38 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139610060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Odontogenic Keratocyst in Maxillary Sinus- A Rare Case Report","authors":"Sabu Paul, L. Cherian, Hafeefa, M, Vally, M, Aishwarya Menon, P, Rasla, P. C","doi":"10.36348/sjpm.2024.v09i01.002","DOIUrl":"https://doi.org/10.36348/sjpm.2024.v09i01.002","url":null,"abstract":"Odontogenic keratocyst is a common and aggressive cystic lesion derived from tooth remnants in the jaw. It is frequently found in the posterior mandible and less common in the maxilla. This report presents a rare case of an odontogenic keratocyst in maxillary sinus which associated with ectopic third molar. Due to its symptomatic resemblance to other maxillary sinus lesions such as sinusitis or antral polyp, pathologists often face challenges in accurately and promptly diagnosing odontogenic keratocyst.","PeriodicalId":471257,"journal":{"name":"Saudi journal of pathology and microbiology","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139623588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Retrospective Study on Mucormycosis and Other Fungal Infections Associated With COVID-19","authors":"Nimmy Paul, Sreekumary Pk, Chithra Chandramohini Nath, Aleena Cyriac, Geethanjali Mohandas, Sajith Kumar, Shibu George","doi":"10.36348/sjpm.2023.v08i12.005","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i12.005","url":null,"abstract":"A drastic increase in mucormycosis and other opportunistic infections was observed during the Covid-19 pandemic owing to multisystem involvement and related Immunosuppression. This retrospective study was carried out among SARS-COV-2 infected patients having invasive fungal infections [IFI] admitted to a tertiary care hospital in the southern part of India, for one year from August 2020. Among a total of 115 suspected cases, 54 patients were diagnosed to have IFI, during or immediately after Covid 19 disease. The most common fungal pathogens isolated were Mucorales (57.4%) followed by Aspergillus spp. (20.3%), Candida spp. (9.2%) and mixed infections (13.1%). Important predisposing factors identified were diabetes mellitus (70.7%), prolonged use of corticosteroids (85%), and administration of broad-spectrum antibiotics (90%). The predominant species identified among the diagnosed mucormycosis cases were Rhizopus spp. (54%) and Mucor spp. (25%); the site of involvement was paranasal sinus (70.1%), and rhino-orbital in 48.7% of the cases. Increased incidence of invasive Aspergillus infection in COVID-19 was seen mainly among ICU patients on ventilators, with Covid-19 induced lung damage. Common presentations were Chronic Pulmonary Aspergillosis, Rhino sinusitis, and Asthma. Of the total IFI, 9.2 % were caused by Candida spp. Of which 75% were due to Non- albicans candida species. Candidemia was the major presentation observed (90%). Of the patients with IFI, 90 % of them recovered, and the rest of them (7.4%) succumbed to infection. Of the patients with rhino-orbital mucormycosis who survived, 10 (39 %) had facial disfigurement and 11 (45 %) had a loss of vision.","PeriodicalId":471257,"journal":{"name":"Saudi journal of pathology and microbiology","volume":"589 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138983097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriological Profile and Antibiogram of Isolates Causing Bloodstream Infection in Children","authors":"Linu Rajeev, Beena V G","doi":"10.36348/sjpm.2023.v08i12.003","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i12.003","url":null,"abstract":"Objective: (1) To identify and characterize bacterial isolates causing blood stream infection in children. (2) To determine the antibiotic sensitivity pattern of isolates obtained. Materials And Methods: Study design: Descriptive study. Study population: Blood samples collected from pediatric patients aged upto 12 years who are admitted in Government Medical College Kottayam, with clinical features of blood stream infection. Sampling methodology: The sample size of suspected blood stream infection is 345. All pediatric patients with clinical features suggestive of blood stream infection in the study period satisfying inclusion and exclusion criteria will be included in the study. The identification of bacterial pathogens and antimicrobial susceptibility tests were done by conventional and automated methods like VITEK system. Results: Out of 345 study sample population, 46 were culture positive, of which 21(45.7%) were Gram negative isolates and 20(43.4%) were Gram positive. Major isolates obtained were Klebsiella spp (15%), Methicillin Resistant Coagulase negative Staphylococci (15%), followed by Staphylococcus aureus (13%), Coagulase negative Staphylococci (8%) and Acinetobacter baumannii (8%). Klebsiella pneumoniae was resistant to most of the antibiotics tested except meropenem. All isolates of Coagulase negative Staphylococci and Staphylococcus aureus were susceptible to vancomycin and linezolid. Conclusion: Gram negative organisms were predominant pathogens in blood stream infections. Klebsiella pneumoniae, Coagulase negative Staphylococci, Staphylococcus aureus were the most commonly isolated pathogens. Amikacin along with the third generation cephalosporins should be used for empirical treatment of Gram negative sepsis. Vancomycin and linezolid can be used for Gram positive pathogens.","PeriodicalId":471257,"journal":{"name":"Saudi journal of pathology and microbiology","volume":"159 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139010695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}