Inhibition Rates of Disinfectants on Bacterial Isolated from Student Dormitory

O. Radhi
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Abstract

Background: Although most of human life is spent inside, where bacteria are always present on things, little is known about the microbial variety of indoor habitats. Objectives: The major objective of this study was to determine the rate and types of bacterial contaminants in female and male student dormitory of Al-Kufa university campus, and to explore the inhibitory effect of certain disinfectants. This study was conducted for the period from October 2022 to Jun 2023. A total of 400 bacteriological swabs were collected aseptically from different sites of male and female student dormitory of Al-Kufa university. Swabs were streaked as soon as possible on blood agar and MaConkey agar plates and incubated overnight. Identification of bacterial species was based on standard bacteriological criteria. The inhibitory effect of 40% and 60% concentrations of three commercially available disinfectants; dettol (chloroxylenol), Al-Emlaq (Alkyl benzene sulfonate), and the house bleach (sodium hypochlorite) were explored by agar diffusion method. Data were statistically analyzed. Results: The results revealed that the overall bacterial contamination rate in student’s dormitories of Al-Kufa university campus was 78%. Male dormitory unit had significantly higher contamination rate compared to female dormitory unit (57.5% vs 42.5%). Bacillus spp. Was the most predominant contaminant in both male and female units. Al-Emlaq super gel (Alkyl benzene sulfonate) was found as the most effective disinfectant against the majority of bacterial isolates. Conclusion: Authorized and public awareness toward bacterial contamination in student's dormitories is recommended. The selection of an effective disinfectant should be based on its active ingredient and concentration.
消毒剂对学生宿舍分离细菌的抑制率
背景:虽然人类生活的大部分时间都是在室内度过的,室内的各种物品上总是存在细菌,但人们对室内栖息地的微生物种类却知之甚少。研究目的本研究的主要目的是确定库法大学校园男女学生宿舍的细菌污染率和种类,并探讨某些消毒剂的抑制效果。研究时间为 2022 年 10 月至 2023 年 6 月。研究人员从库法大学男女学生宿舍的不同地点无菌采集了 400 份细菌拭子。拭子尽快在血琼脂平板和马康凯琼脂平板上作条痕处理并培养过夜。根据标准细菌学标准鉴定细菌种类。采用琼脂扩散法检测了三种市售消毒剂,即 Dettol(氯甲酚)、Al-Emlaq(烷基苯磺酸盐)和家用漂白剂(次氯酸钠)40% 和 60% 浓度的抑菌效果。对数据进行了统计分析。结果结果显示,库法大学校园学生宿舍的总体细菌污染率为 78%。男生宿舍的细菌污染率明显高于女生宿舍(57.5% 对 42.5%)。芽孢杆菌是男女宿舍中最主要的污染物。研究发现,Al-Emlaq 超级凝胶(烷基苯磺酸盐)是对大多数细菌分离物最有效的消毒剂。结论建议对学生宿舍的细菌污染问题进行授权并提高公众意识。有效消毒剂的选择应以其有效成分和浓度为基础。
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