Current Biomedicine最新文献

Optimization of DNA amplification temperature in quantitative polymerase chain reaction for Identification of isoniazid-resistant Mycobacterium tuberculosis 定量聚合酶链反应中 DNA 扩增温度的优化，用于鉴定耐异烟肼结核分枝杆菌

Current Biomedicine Pub Date : 2024-06-15 DOI: 10.29244/currbiomed.2.2.61-70

Dwi Veni Endarwati, Asep Iin Nur Indra, Acep Tantan Hardiana, Yogi Khoirul Abror, Betty Nurhayati, Fusvita Merdekawati

{"title":"Optimization of DNA amplification temperature in quantitative polymerase chain reaction for Identification of isoniazid-resistant Mycobacterium tuberculosis","authors":"Dwi Veni Endarwati, Asep Iin Nur Indra, Acep Tantan Hardiana, Yogi Khoirul Abror, Betty Nurhayati, Fusvita Merdekawati","doi":"10.29244/currbiomed.2.2.61-70","DOIUrl":"https://doi.org/10.29244/currbiomed.2.2.61-70","url":null,"abstract":"Background: Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis and is a serious threat to global health. The methods can be used to detect and identify the bacteria is quantitative polymerase chain reaction (qPCR). In this method, denaturation and extension temperatures are determining factors of success that needs to be optimized. Objective: This study aims to optimize denaturation and extension temperatures in M. tuberculosis DNA amplification. Methods: The research used quasi-experimental design. The denaturation temperature optimized were 93, 94, 95, 96, and 97°C, and the extension temperature optimized were 58, 59, 60, 61, and 62°C. The test sample was a 1 ml sputum sample isolated from a patient with isoniazid-resistant M. tuberculosis. Optimization was performed using seven test primers, namely S315T, S315N, S315I, S315R, S315G, S315L, and R463B with the katG gene target and data analysis using Ms Excel. Data optimization results were processed with Excel by taking the lowest Ct value. Results: The results showed that the optimization temperatures for denaturation were different for each primer used. Primers S315T, S315R, and S315G, optimal with denaturation temperature of 96°C, primer S315N optimal with 94°C, primers S315I and R463B optimal with 93°C, and for primer S315L optimal with 95°C, with the most widely used temperature is 96°C. The optimal extension temperature was 58°C for primers S315T, S315N, S315I, and R463B, at 60°C for primers S315R and S315G, and at 61°C for primer S315L. Conclusion: The optimal denaturation temperature in this study was 96°C and the optimal extension temperature was 58°C.","PeriodicalId":470424,"journal":{"name":"Current Biomedicine","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141337413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Front Matter Vol 2 No 2 正文第 2 卷第 2 期

Current Biomedicine Pub Date : 2024-06-15 DOI: 10.29244/currbiomed.2.2.i-iv

Current Biomedicine

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Back Matter Vol 2 No 2 后记》第 2 卷第 2 期

Current Biomedicine Pub Date : 2024-06-15 DOI: 10.29244/currbiomed.2.2.v-xi

Current Biomedicine

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Hipertrofi sel goblet pada usus halus ayam kampung di pasar tradisional Jakarta yang terinfeksi cacing cestoda 雅加达传统市场中感染绦虫的土鸡小肠内腺泡细胞肥大

Current Biomedicine Pub Date : 2024-06-15 DOI: 10.29244/currbiomed.2.2.93-100

Monica Silva Jerica, R. Tiuria, Nilla Mayasari, A. Nugraha, Mawar Subangkit

{"title":"Hipertrofi sel goblet pada usus halus ayam kampung di pasar tradisional Jakarta yang terinfeksi cacing cestoda","authors":"Monica Silva Jerica, R. Tiuria, Nilla Mayasari, A. Nugraha, Mawar Subangkit","doi":"10.29244/currbiomed.2.2.93-100","DOIUrl":"https://doi.org/10.29244/currbiomed.2.2.93-100","url":null,"abstract":"Latar Belakang: Ayam kampung merupakan salah satu kebutuhan protein hewani yang sering dicari masyarakat. Pemeliharaan ayam kampung dengan sistem sangkar bebas menjadi predisposisi terinfeksi oleh parasit gastrointestinal. Tujuan: Penelitian ini bertujuan mengetahui perubahan sel epitel dan hipertrofi sel goblet saluran pencernaan pada ayam kampung yang terinfeksi cacing cestoda Railletina spp secara alami. Metode: Penelitian ini menggunakan tujuh preparat arsip histopatologi usus halus ayam kampung yang terinfeksi cacing cestoda. Sampel usus halus ayam kampung diambil dari dua pasar yang berbeda yaitu pasar Pluit, Jakarta Utara dan pasar Kebayoran lama, Jakarta Selatan. Hasil: Hasil pengamatan secara histopatologi pada preparat usus ditemukan adanya deskuamasi epitel vili dan proliferasi sel-sel kripta yang diakibatkan oleh infeksi cacing cestoda. Jumlah sel goblet yang mengalami hipertrofi paling banyak ditemukan pada sampel preparat yang diambil dari pasar Kebayoran Lama, Jakarta Selatan walaupun tidak berpengaruh secara signifikan (P > 0,05) terhadap infeksi cestoda. Simpulan: Berdasarkan hasil penelitian ini dapat disimpulkan bahwa deskuamasi vili dan peningkatan jumlah hipertrofi sel goblet terjadi akibat infeksi cacing cestoda pada mukosa usus halus ayam kampung.","PeriodicalId":470424,"journal":{"name":"Current Biomedicine","volume":"3 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141336446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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In vitro and in vivo effects of curcumin on oral cancer: a systematic review 姜黄素对口腔癌的体外和体内影响：系统综述

Current Biomedicine Pub Date : 2024-06-15 DOI: 10.29244/currbiomed.2.2.101-115

Fiki Muhammad Ridho, Andika Julyanto Syachputra, Panggih Fahrudin, Andang Nurhuda, N. Nurliana, Nadhia S. Latuamury

{"title":"In vitro and in vivo effects of curcumin on oral cancer: a systematic review","authors":"Fiki Muhammad Ridho, Andika Julyanto Syachputra, Panggih Fahrudin, Andang Nurhuda, N. Nurliana, Nadhia S. Latuamury","doi":"10.29244/currbiomed.2.2.101-115","DOIUrl":"https://doi.org/10.29244/currbiomed.2.2.101-115","url":null,"abstract":"Background: Current therapy for oral cancer (OC) patients, including surgery, radiotherapy, and chemotherapy, still have many shortcomings. Therefore, the discovery of natural products to prevent and treat cancer is receiving increasing attention, one of which is curcumin. Curcumin (diferuloylmethane) is a polyphenolic compound found in turmeric (Curcuma longa) and has been widely used as a herbal medicine because of its effects on health, one of which is as an anticancer agent. Objective: This study aimed to systematically and comprehensively review and summarize the anticancer effects and mechanisms of action involved of curcumin on OC cells. Methods: A systematic review methodology was employed adhering to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines to review and summarize previous studies published in databases, including PubMed, ScienceDirect, and Google Scholar. The final results included 14 articles, both in vitro and in vivo studies. Results: Based on several preclinical studies regarding the effects of curcumin on OC cells, we highlight that curcumin has a strong potential in inhibiting OC cells through exerted effects such as immunomodulatory and anti-inflammatory effects, through inhibition of cell proliferation, invasion and migration, and angiogenesis, as well as through the induction of apoptosis and autophagy. Conclusion: The systematic review presented in this paper concludes that curcumin possesses the potential to inhibit the development of OC cells through several mechanisms of action related to immunomodulatory effects, anti-inflammatory effects, cell proliferation, invasion and migration, angiogenesis, apoptosis, and autophagy.","PeriodicalId":470424,"journal":{"name":"Current Biomedicine","volume":"4 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141337300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Acute toxicity test of avocado (Persea americana) oil in mice 鳄梨（Persea americana）油对小鼠的急性毒性试验

Current Biomedicine Pub Date : 2024-06-15 DOI: 10.29244/currbiomed.2.2.55-60

Aisya Salsa Bhila, Andriyanto Andriyanto, Bayu Febram Prasetyo

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The effect of reusing formaldehyde fixative solution on the quality of histopathological slides and the amount of waste produced 重复使用甲醛固定液对组织病理切片质量和废物产生量的影响

Current Biomedicine Pub Date : 2024-06-15 DOI: 10.29244/currbiomed.2.2.71-83

Zon Hardi, Wiwin Wiryanti, Adang Durachim, Mamat Rahmat

{"title":"The effect of reusing formaldehyde fixative solution on the quality of histopathological slides and the amount of waste produced","authors":"Zon Hardi, Wiwin Wiryanti, Adang Durachim, Mamat Rahmat","doi":"10.29244/currbiomed.2.2.71-83","DOIUrl":"https://doi.org/10.29244/currbiomed.2.2.71-83","url":null,"abstract":"Background: Neutral buffered formalin (NBF) 10% fixative solution is widely used in histopathological slides. The fixation process generates liquid waste of NBF 10% and solid waste of tissue remnants. Objective: The research aimed to assess the reuse of NBF 10% fixative solution on the quality of histopathological slides and calculate the amount of waste produced. Methods: Treatments included single-use of fixative solution (control), reuse for 1, 2, and 3 times. Ten sample slides were prepared for each treatment, consisting of intestinal tissue, uterine fibroids, prostate, uterus, ovarian cyst, portio vaginalis cervicis, thyroid, rectum, breast fibroadenoma, and gallbladder tissues. Tissues were fixed with NBF 10% and processed histologically with hematoxylin-eosin staining. Liquid waste of NBF 10% and solid waste of tissue remnants were quantified. Histopathological slide quality was measured under a microscope for nuclear and cytoplasmic clarity, staining intensity, and color uniformity. Results: Control slides exhibited good quality with clearly blue-stained nuclei, pink cytoplasm, no color accumulation, and uniform staining across fields of view. Reused NBF 10% slides experienced a decrease in quality compared to the control but were still usable for diagnosis. Slides reused 2 and 3 times showed poor quality, making diagnosis difficult. Fixation resulted in 299.0 liters of liquid waste of NBF 10% and 64.9 kilograms of solid tissue remnants. Conclusion: Reusing NBF 10% decreases histological slide quality, though reuse once still allows for diagnosis. Reusing 10% NBF for tissue fixation can reduce the liquid waste of fixative solution and solid tissue waste.","PeriodicalId":470424,"journal":{"name":"Current Biomedicine","volume":"5 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141336019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Concentration and purity of DNA extraction with sonication and spin column methods from the sputum sample of tuberculosis patient 从肺结核患者痰液样本中用超声法和旋柱法提取 DNA 的浓度和纯度

Current Biomedicine Pub Date : 2024-06-15 DOI: 10.29244/currbiomed.2.2.84-92

Fitrianingsih Saputra, Asep Iin Nur Indra, Ai Djuminar, Fusvita Merdekawati, Betty Nurhayati

{"title":"Concentration and purity of DNA extraction with sonication and spin column methods from the sputum sample of tuberculosis patient","authors":"Fitrianingsih Saputra, Asep Iin Nur Indra, Ai Djuminar, Fusvita Merdekawati, Betty Nurhayati","doi":"10.29244/currbiomed.2.2.84-92","DOIUrl":"https://doi.org/10.29244/currbiomed.2.2.84-92","url":null,"abstract":"Background: The Polymerase Chain Reaction (PCR) method can identify Mycobacterium tuberculosis in a sputum sample of a patient with TB (TB). One crucial step to ensure accurate PCR results is the DNA extraction process. Objective: The research aims to compare the concentration and purity of DNA from the sputum of TB patients using ultrasound and spin column extraction techniques. Methods: The research uses descriptive study designs with post-only design strategies. The primary data was derived from 18 sputum specimens from TB patients. Concentration measurement and DNA purity testing using a nanodrop spectroscopic photometer. Results: DNA extraction by ultrasound method has an average concentration of 18.9 ± 8.5 ng/L, with a peak of 37.6 ng/ L. The spin column method produces an average of 55.5 ± 27.9 ng/μL; the peak is 105.0 ng/ μL. The purity value of the DNA extract is in the range of 1.8 ± 2.0 with the ultrasound method of 61% and the spin column of 78%. Conclusion: The sonication method has a lower average concentration and a higher percentage of purity than the spin column method, and there are differences in concentrations and purity values between the two methods.","PeriodicalId":470424,"journal":{"name":"Current Biomedicine","volume":"8 47","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141337702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Front Matter Vol 2 No 1 正文第 2 卷第 1 期

Current Biomedicine Pub Date : 2024-02-23 DOI: 10.29244/currbiomed.2.1.i-iii

Admin Currbiomed

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Front Matter 前页

Current Biomedicine Pub Date : 2023-10-05 DOI: 10.29244/currbiomed.1.2.i-iv

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