The effect of reusing formaldehyde fixative solution on the quality of histopathological slides and the amount of waste produced

Current Biomedicine Pub Date : 2024-06-15 DOI:10.29244/currbiomed.2.2.71-83

Zon Hardi, Wiwin Wiryanti, Adang Durachim, Mamat Rahmat

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Abstract

Background: Neutral buffered formalin (NBF) 10% fixative solution is widely used in histopathological slides. The fixation process generates liquid waste of NBF 10% and solid waste of tissue remnants. Objective: The research aimed to assess the reuse of NBF 10% fixative solution on the quality of histopathological slides and calculate the amount of waste produced. Methods: Treatments included single-use of fixative solution (control), reuse for 1, 2, and 3 times. Ten sample slides were prepared for each treatment, consisting of intestinal tissue, uterine fibroids, prostate, uterus, ovarian cyst, portio vaginalis cervicis, thyroid, rectum, breast fibroadenoma, and gallbladder tissues. Tissues were fixed with NBF 10% and processed histologically with hematoxylin-eosin staining. Liquid waste of NBF 10% and solid waste of tissue remnants were quantified. Histopathological slide quality was measured under a microscope for nuclear and cytoplasmic clarity, staining intensity, and color uniformity. Results: Control slides exhibited good quality with clearly blue-stained nuclei, pink cytoplasm, no color accumulation, and uniform staining across fields of view. Reused NBF 10% slides experienced a decrease in quality compared to the control but were still usable for diagnosis. Slides reused 2 and 3 times showed poor quality, making diagnosis difficult. Fixation resulted in 299.0 liters of liquid waste of NBF 10% and 64.9 kilograms of solid tissue remnants. Conclusion: Reusing NBF 10% decreases histological slide quality, though reuse once still allows for diagnosis. Reusing 10% NBF for tissue fixation can reduce the liquid waste of fixative solution and solid tissue waste.

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重复使用甲醛固定液对组织病理切片质量和废物产生量的影响

背景：中性缓冲福尔马林（NBF）10% 固定液广泛用于组织病理切片。固定过程中会产生 10% NBF 的液体废物和组织残余的固体废物。研究目的研究旨在评估 10%NBF 固定液的再利用对组织病理切片质量的影响，并计算产生的废物量。研究方法处理方法包括一次性使用固定液（对照组）、重复使用 1 次、2 次和 3 次。每次处理准备 10 张样本切片，包括肠组织、子宫肌瘤、前列腺、子宫、卵巢囊肿、宫颈阴道口、甲状腺、直肠、乳腺纤维腺瘤和胆囊组织。组织用 10%的 NBF 固定，并用苏木精-伊红染色进行组织学处理。10% NBF 的液体废物和组织残余的固体废物均被量化。在显微镜下测量组织病理切片的质量，包括细胞核和细胞质的清晰度、染色强度和颜色均匀性。结果对照组载玻片显示出良好的质量，细胞核蓝色染色清晰，细胞质呈粉红色，无颜色积累，各视野染色均匀。与对照组相比，重复使用的 10%NBF玻片质量有所下降，但仍可用于诊断。重复使用 2 次和 3 次的玻片质量较差，难以诊断。固定过程中产生了 299.0 升的 10% NBF 废液和 64.9 千克的固体组织残留物。结论重复使用 10% NBF 会降低组织切片的质量，但重复使用一次仍可用于诊断。重复使用 10% NBF 进行组织固定可减少固定液的液体浪费和固体组织的浪费。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Current Biomedicine

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