Journal of Acupuncture and Meridian Studies最新文献

{"title":"Effect of Acupressure at PC6 on Nausea and Vomiting During Pregnancy: a Randomized Controlled Trial.","authors":"Melike Pündük Yılmaz,&nbsp;Saadet Yazıcı,&nbsp;İsmail Yılmaz","doi":"10.51507/j.jams.2023.16.3.89","DOIUrl":"https://doi.org/10.51507/j.jams.2023.16.3.89","url":null,"abstract":"<p><strong>Background: </strong>Nausea and vomiting are among the most common medical problems during pregnancy, affecting 80% of mothers.</p><p><strong>Objectives: </strong>This study was conducted as a randomized controlled experimental study to determine the effect of acupressure applied by a wristband to the pericardium 6 (PC6) point on nausea and vomiting during pregnancy.</p><p><strong>Methods: </strong>The study population consisted of 74 pregnant women between 6-14 weeks of gestation who were experiencing nausea and vomiting. The study data was collected with personal information from the Pregnancy-Unique Quantification of Emesis Scale (PUQE). Experimental and control groups were selected by a simple random method. The experimental group wore acupressure wristbands for one week, while the control group did not use any method to alleviate nausea and vomiting. One week later, the PUQE scale was applied to both groups.</p><p><strong>Results: </strong>The acupressure wristbands decreased the nausea and vomiting scores of the pregnant women in the experimental group without statistical significance but did not change the nausea and vomiting scores of the pregnant women in the control group.</p><p><strong>Conclusion: </strong>Acupressure wristbands can be used to prevent nausea and vomiting during pregnancy.</p>","PeriodicalId":46854,"journal":{"name":"Journal of Acupuncture and Meridian Studies","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9696353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Biophysical Properties of Meridians in the Visualization of Pericardium Meridian.","authors":"Feng Xiong,&nbsp;Ruimin Xu,&nbsp;Tongju Li,&nbsp;Jinyu Wang,&nbsp;Qingchuan Hu,&nbsp;Xiaojing Song,&nbsp;Guangjun Wang,&nbsp;Huanhuan Su,&nbsp;Shuyong Jia,&nbsp;Shuyou Wang,&nbsp;Zongxiang Zhu,&nbsp;Weibo Zhang","doi":"10.51507/j.jams.2023.16.3.101","DOIUrl":"https://doi.org/10.51507/j.jams.2023.16.3.101","url":null,"abstract":"<p><strong>Background: </strong>The biophysical properties of the meridian system, an important concept of traditional Chinese medicine, include low impedance, resounding voice, and high acoustic conductance, all of which are helpful for elucidating the essence of meridians.</p><p><strong>Objectives: </strong>To visualize the human pericardium meridian (PC) based on the resounding voice property of meridians.</p><p><strong>Methods: </strong>Visualization of the PC was performed by injection of fluorescein sodium at the PC6 acupoint (Neiguan) on the PC. Before injection, percussion active points (PAPs) were identified by the virtue of their resounding voice properties. After injection, the trajectories of fluorescein migration throughout the body surface were recorded and analyzed. The distribution of fluorescein in the tissue was further studied using cross-sections of hind limbs of mini-pigs, in which fluorescein was injected into low impedance points.</p><p><strong>Results: </strong>The identified PAP lines were colocalized with PC. Following intradermal fluorescein injection, 1-3 fluorescent lines, which were unrelated to the arm veins, were observed in 7 of 10 participants; 85.4% of fluorescent signals were coincident with PAPs and their intensity had a negative correlation with the body mass index (r = -0.56, <i>p</i> = 0.045). Cross-sections showed a Y-shaped fluorescence pattern where the two migration lines on the surface were the two vertices of the \"Y.\"</p><p><strong>Conclusion: </strong>The trajectories of fluorescein in the body are suggestive of the anatomical structure of meridians. The PC is related to the deep horizontal interstitial channels that connect to the body surface through vertical interstitial spaces. These biophysical properties and techniques for meridian visualization are valuable for revealing the anatomical structure of meridians.</p>","PeriodicalId":46854,"journal":{"name":"Journal of Acupuncture and Meridian Studies","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9696351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acupuncture in the Anesthetic Recovery of Bitches Submitted to Ovariohysterectomy.","authors":"Amanda Caroline Gomes Graboschii,&nbsp;Carolina Carvalho Dos Santos Lira,&nbsp;Jackellyne Laís Ferreira Lins,&nbsp;Marcia Kikuyo Notomi,&nbsp;Pierre Barnabé Escodro","doi":"10.51507/j.jams.2023.16.3.95","DOIUrl":"https://doi.org/10.51507/j.jams.2023.16.3.95","url":null,"abstract":"<p><strong>Background: </strong>Cardiorespiratory depression caused by anesthesia decreases the quality and increases the time of postoperative recovery. The acupoint Governor Vessel 26 (GV26) is a resuscitation point that can reverse this depression and can be safely used without side effects.</p><p><strong>Objectives: </strong>The objective of this study was to evaluate the stimulation and anesthetic recovery time of GV26 in bitches submitted to ovariohysterectomy (OH) under dissociative anesthesia.</p><p><strong>Methods: </strong>As pre-anesthetic protocol, acepromazine 0.2% (0.1 mg/kg) and tramadol hydrochloride (2 mg/kg) was used, and induction was performed using midazolam (0.5 mg/kg) and ketamine (10 mg/kg). For the control group, standard procedure was performed for OH, with anesthetic recovery and post-surgical procedures. For the acupuncture group (AP), the stimulation of acupoint GV26 was performed 20 minutes after the anesthetic induction and maintained for 5 minutes. Respiratory rate, amplitude (superficial, normal or deep), type of respiratory movement (abdominal, abdominocostal or thoracoabdominal), heart rate, capillary filling time, temperature, presence or absence of laryngotracheal reflex, presence or absence of interdigital reflexes were assessed immediately before PAM application, and 2 (T1), 5 (T2), 10 (T3), 15 (T4), 20 (T5), 25 (T6) and 30 (T7) minutes after treatment. The results were tabulated and statistically analyzed.</p><p><strong>Results: </strong>When comparing the AP group with the control group, an improvement in amplitude of the chest cage was observed at all times, where the animals remained in normal or deep respiratory amplitude. The heart rate was significantly higher for the AP group (155.5 ± 34.4 bpm) than the control group at T1 (105.1 ± 15.4 bpm), while recovery time was lower for the AP group (54.1 ± 14.9 min) when compared to control group (79.9 ± 17.9 min).</p><p><strong>Conclusion: </strong>The present paper demonstrated the efficacy of GV26 in maintaining adequate respiratory amplitude and decreasing the anesthetic recovery time.</p>","PeriodicalId":46854,"journal":{"name":"Journal of Acupuncture and Meridian Studies","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9696352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ziwuliuzhu Acupuncture Modulates Clock mRNA, Bmal1 mRNA and Melatonin in Insomnia Rats.","authors":"Ao Huang,&nbsp;Gefang Xiao,&nbsp;Yiliu Chen,&nbsp;Zuying Hu,&nbsp;Pin-Hsuan Lee,&nbsp;Yusen Huang,&nbsp;Zifeng Zhuang,&nbsp;Yuling Zhang,&nbsp;Peng Qing,&nbsp;Canghuan Zhao","doi":"10.51507/j.jams.2023.16.3.109","DOIUrl":"https://doi.org/10.51507/j.jams.2023.16.3.109","url":null,"abstract":"<p><strong>Background: </strong>In clinics, Ziwuliuzhu acupuncture is widely considered an effective method of treating insomnia; however, there is currently limited information available regarding its possible mechanisms. Although the method of Ziwuliuzhu acupuncture possesses a unique rhythmic pattern.</p><p><strong>Objectives: </strong>In this study, we have creatively combined the traditional Chinese medicine of Ziwuliuzhu with a modern biological rhythm to investigate the internal mechanism of insomnia.</p><p><strong>Methods: </strong>Pathological tissue from the hypothalamus was analyzed using hematoxylin-eosin staining. The level of TNF (tumor necrosis factor)-α in the SCN (suprachiasmatic nucleus) area of the hypothalamus was detected in situ using the TUNEL fluorescence staining assay. The concentration of hypothalamic melatonin was detected using the enzyme-linked immunosorbent assay (ELISA). The mRNA expression of Clock and Bmal1 was measured using RT-qPCR.</p><p><strong>Results: </strong>In the Ziwuliuzhu acupuncture groups, the structural damage in the hypothalamic neurons was alleviated compared to the model group and the expression of inflammatory factors was reduced. The mRNA expression levels of Clock and Bmal1 were significantly increased (<i>p</i> < 0.05). The concentration of melatonin was significantly increased (<i>p</i> < 0.001). Although there were no significant differences between the treatment groups (diazepam group, Nazi group, Najia group, and routine group) (<i>p</i> > 0.05).</p><p><strong>Conclusion: </strong>Ziwuliuzhu acupuncture alleviated neuronal damage and modulated the inflammatory reaction in the hypothalamus of rats with insomnia. Moreover, Ziwuliuzhu acupuncture increased the expression levels of Clock and Bmal1 mRNA, and MT content. This study has potentially highlighted one of the mechanisms through which Ziwuliuzhu acupuncture can be used to treat insomnia.</p>","PeriodicalId":46854,"journal":{"name":"Journal of Acupuncture and Meridian Studies","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10119115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ACURATE: a Guide for Reporting Sham Controls in Trials Using Acupuncture.","authors":"Ye-Seul Lee,&nbsp;Song-Yi Kim,&nbsp;Hyangsook Lee,&nbsp;Younbyoung Chae,&nbsp;Myeong Soo Lee","doi":"10.51507/j.jams.2023.16.3.119","DOIUrl":"https://doi.org/10.51507/j.jams.2023.16.3.119","url":null,"abstract":"<p><p>This paper presents the Acupuncture Controls gUideline for Reporting humAn Trials and Experiments (ACURATE) checklist, an extension of The Consolidated Standards for Reporting of Trials (CONSORT), which is to be used with STandards for Reporting Interventions in Clinical Trials of Acupuncture (STRICTA) when real and sham acupuncture needles are used in a study. This checklist focuses on a clear depiction of sham needling procedures to enhance replicability and enable a precise appraisal. We encourage researchers to use ACURATE in trials and reviews involving sham acupuncture to assist in the reporting of sham acupuncture procedures and related components.</p>","PeriodicalId":46854,"journal":{"name":"Journal of Acupuncture and Meridian Studies","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9701669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abdominal Acupuncture for Non-Responding TMD Patients: a Retrospective Observational Study in General Practice.","authors":"Emanuela Serritella,&nbsp;Gabriella Galluccio,&nbsp;Carlo Di Paolo","doi":"10.51507/j.jams.2023.16.2.56","DOIUrl":"https://doi.org/10.51507/j.jams.2023.16.2.56","url":null,"abstract":"<p><strong>Background: </strong>Pain related to Temporomandibular Disorders (TMD) is severe, negatively affecting patients' quality of life, and often resistant to conventional treatments. Abdominal Acupuncture (AA) is known to be particularly effective for pain, especially chronic and musculoskeletal pain, but it is still poorly studied and never investigated in TMD patients.</p><p><strong>Objectives: </strong>To analyze the efficacy of AA for the treatment of patients with subacute and chronic pain related to TMD and non-responding to previous conventional therapies (occlusal splint, medications, physical therapy).</p><p><strong>Methods: </strong>Twenty-eight patients, 24 F and four M (mean age 49.36 years), were recruited from January 2019-February 2021. All patients underwent AA treatment: two sessions per week for four weeks, for a total of eight sessions. At the beginning of therapy (T0) and at the end of the cycle (T1) the following data were evaluated: maximum mouth opening (MMO); cranio-facial pain related to TMD (verbal numeric scale, VNS); pain interference with normal activities and quality of life of patients (Brief Pain Inventory, BPI); oral functioning (Oral Behavior Checklist, OBC); impression of treatment effectiveness (Patients' Global Impression of Improvement, PGI-I Scale). Statistical comparison of data before and after the AA treatment was performed by Wilcoxon's signed-rank test (significance level <i>p</i> < 0.05).</p><p><strong>Results: </strong>The MMO values were significantly improved after one cycle of AA (<i>p</i> = 0.0002). In addition, TMD-related pain had a statistically significant decline following AA treatment (all <i>p</i> < 0.001). Patients' general activity and quality of life (BPI) were described as improved following a course of AA, with statistically significant values for all aspects considered (all <i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>Abdominal acupuncture resulted in effective treatment of subacute/chronic-resistant pain related to TMD, capable of improving mandibular function and facial pain, and reduced the interference of pain affecting patients' quality of life.</p>","PeriodicalId":46854,"journal":{"name":"Journal of Acupuncture and Meridian Studies","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9772772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Pharmacopuncture Therapy in Adhesive Capsulitis: a Study Protocol for a Pragmatic Randomized Controlled Trial.","authors":"Hui Yan Zhao, Chang-Hyun Han, Changsop Yang, Yoon Jae Lee, In-Hyuk Ha, Kyoung Sun Park","doi":"10.51507/j.jams.2023.16.2.70","DOIUrl":"10.51507/j.jams.2023.16.2.70","url":null,"abstract":"<p><strong>Background: </strong>Adhesive capsulitis is a progressive, idiopathic disorder that significantly impacts individuals̓ daily lives and increases their medical burden. Pharmacopuncture therapy, which combines acupuncture techniques with herbal medicine, involves injecting herbal extracts into specific acupoints. This study aims to determine the effectiveness and safety of pharmacopuncture therapy in comparison to physiotherapy (PT) for treating adhesive capsulitis.</p><p><strong>Methods: </strong>This research protocol outlines a two-arm, parallel, multi-center, pragmatic randomized controlled trial. Fifty participants will be randomly allocated to either the pharmacopuncture therapy or PT group, and they will receive 12 sessions of their respective therapies over a 6-week period. The primary outcome measure is the numeric rating scale for shoulder pain. Secondary outcomes include the visual analog scale score for shoulder pain, Shoulder Pain and Disability Index, Patients̓ Global Impression of Change score, Short Form-12 Health Survey Version 2 score, and EuroQol-5 Dimension. Statistical analysis will be conducted based on the intention-to-treat principle.</p><p><strong>Discussion: </strong>This trial may offer high-quality and reliable clinical evidence for evaluating the effectiveness and safety of pharmacopuncture therapy compared to PT in the treatment of adhesive capsulitis. Furthermore, this study will serve as a valuable guideline for practitioners when making clinical decisions and managing adhesive capsulitis.</p>","PeriodicalId":46854,"journal":{"name":"Journal of Acupuncture and Meridian Studies","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9789547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative Strategy with Ayurveda and Electro-Acupuncture in Hemifacial Spasm: a Case Report.","authors":"Akshatha K Bhat,&nbsp;Venugopalan Krishna Kumar,&nbsp;Jim Daniel Johnson","doi":"10.51507/j.jams.2023.16.2.65","DOIUrl":"https://doi.org/10.51507/j.jams.2023.16.2.65","url":null,"abstract":"<p><p>We report a primary hemifacial spasm that started four years ago with sudden twitching of the face towards the right side. It was diagnosed as a hemifacial spasm by a neurologist and prescribed with Zeptol 100 mg 0.5 tablet BID for two weeks, followed by two sittings of Botox injection in a gap of 1 year. A year later, it reappeared more severely, driving her towards an integrative treatment modality. Ayurveda treatments including <i>Nasya</i>, <i>Ksheera dhooma</i>, internal medications, and <i>Rasona navaneetha prayoga</i> were administered. GV20, GB14, EX-HN5, ST3, ST4, ST6, TE17, LI4, and GB34 were selected for electro-acupuncture. The scores of hemifacial spasm grading and quality of life scale were 9 and 20 (before), 6 and 16 (after treatment), and 4 and 10 (follow-up after six months), respectively. This integrative approach was safe and has shown an improvement in hemifacial spasm.</p>","PeriodicalId":46854,"journal":{"name":"Journal of Acupuncture and Meridian Studies","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9789545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness and Safety of Adjunctive Pharmacopuncture to Acupuncture Treatment for Rotator Cuff Diseases: a Protocol for an Assessor-Blinded, Pragmatic Randomized Controlled, Pilot Trial.","authors":"Hyun Ji Cha,&nbsp;Chang-Hyun Han,&nbsp;Ju Hyun Jeon,&nbsp;Jeong Kyo Jeong,&nbsp;Hong Kyoung Kim,&nbsp;Changsop Yang,&nbsp;Byoung-Kab Kang,&nbsp;Min Ji Kim,&nbsp;Young Eun Choi,&nbsp;In-Hyuk Ha,&nbsp;Young Il Kim","doi":"10.51507/j.jams.2023.16.2.79","DOIUrl":"https://doi.org/10.51507/j.jams.2023.16.2.79","url":null,"abstract":"<p><strong>Background: </strong>Shoulder pain is a common musculoskeletal disorder. Treatment can be surgical or non-surgical. Korean Medicine, including acupuncture and pharmacopuncture, is a part of conservative treatment. Pharmacopuncture, combining acupuncture with herbal medicine, has been used for musculoskeletal disorders since the 1960s, but clinical evidence on its effectiveness is lacking.</p><p><strong>Objectives: </strong>This study aims to assess the effectiveness and safety of pharmacopuncture for rotator cuff disease.</p><p><strong>Methods: </strong>A two-group, parallel, single-center, pragmatic, randomized, controlled, assessor-blinded trial will be conducted. A total of 40 patients will be recruited, starting in July 2022. All patients will be received acupuncture treatment, and pharmacopuncture will be applied to intervention group additionally. After eight treatments are delivered over four weeks, follow-up assessments will be performed.</p><p><strong>Results: </strong>Assessments will evaluate the effectiveness and safety of these treatments at baseline and at weeks 2 (2 W), 4 (4 W), and 8 (8 W). The primary outcome will be a visual analog scale (VAS) evaluation of shoulder pain levels. Assessments will include shoulder pain and disability index (SPADI), shoulder range of motion (ROM), EuroQol 5-Dimension 5-Level (EQ-5D-5L), patient global impression of change (PGIC), 'no worse than mild pain', and drug consumption rates.</p><p><strong>Conclusion: </strong>This study may offer a rationale for a future full-scale trial on the effectiveness and safety of pharmacopuncture treatment for rotator cuff disease and provide data on non-surgical treatment for the disease.</p>","PeriodicalId":46854,"journal":{"name":"Journal of Acupuncture and Meridian Studies","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9789543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Manual Acupuncture on Mitochondrial Fusion and Fission Gene Expression in Rat Spleen.","authors":"Yu-Mi Lee,&nbsp;Dong-Hee Choi,&nbsp;Jeong-Hye Park,&nbsp;Min-Woo Cheon,&nbsp;Jae Gwan Kim,&nbsp;Jeong-Sang Kim,&nbsp;Taejin Choi,&nbsp;Hye-Ran Kim,&nbsp;Daehwan Youn","doi":"10.51507/j.jams.2023.16.2.49","DOIUrl":"https://doi.org/10.51507/j.jams.2023.16.2.49","url":null,"abstract":"<p><strong>Background: </strong>A significant amount of research has been conducted to establish the validity of acupuncture, and it has been demonstrated through animal disease model studies that acupuncture influences mitochondrial changes. However, to more accurately examine the mechanisms of acupuncture treatment effectiveness in pathological models, it is crucial to investigate changes in disease-free animals. Among various hypotheses regarding the effects of acupuncture on the body, we focused on the result that acupuncture stimulation is related to mitochondria.</p><p><strong>Objectives: </strong>We examined the effects of acupuncture mitochondrial fission and fusionrelated mediators in disease-free Sprague Dawley (SD) rats' spleen meridian acupoints.</p><p><strong>Methods: </strong>SD rats were divided into control, SP1, SP2, SP3, SP5, and SP9 acupuncture groups. Acupuncture was performed at each point for 10 minutes daily for four days. Peroxisome proliferator-activated receptor-gamma coactivator 1-α (<i>PGC-1α</i>) and fission protein 1 (<i>Fis1</i>) levels were evaluated using quantitative real-time polymerase chain reaction (<i>qRT-PCR</i>), while dynamin-related protein 1 (<i>DRP1</i>), optic atrophy-1 (<i>OPA1</i>), mitofusin-1 (<i>MFN1</i>), and mitofusin-2 (<i>MFN2</i>) levels were assessed via western blotting. Mitochondria protein concentrations and NADH dehydrogenase activity in spleen tissues were measured using enzyme-linked immunosorbent assay (<i>ELISA</i>).</p><p><strong>Results: </strong>PGC-1α expression decreased in the SP1 (<i>p</i> < 0.01), SP5 (<i>p</i> < 0.05), and SP9 (<i>p</i> < 0.05) groups, while <i>Fis1</i> expression increased in the SP1 (<i>p</i> < 0.01), SP5 (<i>p</i> < 0.01), and SP9 (<i>p</i> < 0.05) groups. <i>DRP1</i>, <i>OPA1</i>, <i>MFN1</i>, and <i>MFN2</i> levels exhibited no significant changes. Mitochondrial protein concentrations decreased in the SP2 (<i>p</i> < 0.01), SP3 (<i>p</i> < 0.01), SP5 (<i>p</i> < 0.01), and SP9 (<i>p</i> < 0.01) groups, while NADH dehydrogenase activity decreased in the SP2 (<i>p</i> < 0.05) and SP9 (<i>p</i> < 0.05) groups.</p><p><strong>Conclusion: </strong>Acupuncture at the SP9 acupoint influenced the mitochondrial fission pathway by modulating <i>PGC-1α</i> and <i>Fis1</i> mediators in the rat spleen under non-disease conditions.</p>","PeriodicalId":46854,"journal":{"name":"Journal of Acupuncture and Meridian Studies","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9772771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}